Ratiometric electrochemical biosensor based on Cu(II) modified covalent organic framework for the ultra-sensitive and specific detection of glutathione

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 492, P. 152271 - 152271

Published: May 15, 2024

Language: Английский

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Bioinspired Iron Porphyrin Covalent Organic Frameworks-Based Nanozymes Sensor Array: Machine Learning-Assisted Identification and Detection of Thiols

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(51), P. 71048 - 71059

Published: Dec. 12, 2024

Given the crucial role of thiols in maintaining normal physiological functions, it is essential to establish a high-throughput and sensitive analytical method identify quantify various accurately. Inspired by iron porphyrin active center natural horseradish peroxidase (HRP), we designed synthesized two covalent organic frameworks (Fe-COF-H Fe-COF-OH) with notable peroxidase-like (POD) activity, capable catalyzing 3,3′,5,5′-tetramethylbenzidine (TMB) into oxidized TMB three distinct absorption peaks. Based on these, six-channel nanozyme colorimetric sensor array was constructed, which could map specific fingerprints thiols. Subsequently, machine learning techniques, including supervised linear discriminant analysis (LDA), decision trees (DT) artificial neural networks (ANN), unsupervised hierarchical cluster (HCA), ensemble random forests (RF), were used for precise identification complex systems, detection limit as low 50 nM. Significantly, demonstrated strong potential practical applications, analyzing homocysteine (Hcy) human serum, mercaptoacetic acid (TGA) depilatory creams, glutathione (GSH) cell lysates, thereby showing promise use disease diagnosis.

Language: Английский

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Lipopolysaccharide affects metabolic processes and energy homeostasis in the corpus luteum

Frontiers in Molecular Biosciences, Journal Year: 2025, Volume and Issue: 11

Published: Jan. 7, 2025

Chronic inflammation caused by Escherichia coli infections has a significant negative impact on the reproductive system and impairs fertility. The corpus luteum (CL) plays central role not only in regulating ovary cycle, but also implantation of embryo maintenance early pregnancy through secretion progesterone. Understanding intricate interplay between inflammatory processes organ's function is crucial for development effective therapeutic strategies to alleviate disorders improve aim this study was determine vitro effects lipopolysaccharide (LPS) proteomic profile porcine CL mid-luteal phase estrous cycle using LC-MS/MS analysis. slices were incubated presence LPS 24 h. We identified 12 differentially regulated proteins after treatment with (7 them upregulated, while 5 downregulated). analysis showed that these are involved such as glucose metabolism, tricarboxylic acid (TCA), detoxification well steroid biosynthesis CL. Moreover, we demonstrated decreases levels increases progesterone These findings suggest modulates key metabolic pathways CL, potentially impacting its functional activity.

Language: Английский

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Establishing reference intervals for serum glutathione reductase in healthy adults

Archives of Medical Science, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 28, 2025

Introduction Glutathione reductase (GR) is a key enzyme that catalyzes the reduction of oxidized glutathione to its reduced form, playing vital role in protecting cells from oxidative damage caused by accumulation reactive oxygen species. This study employed ultraviolet enzymatic method measure serum GR activity, aiming investigate distribution among apparently healthy adults and preliminarily establish reference intervals for GR. Material methods A total 6,180 individuals participated this study. Serum activity was measured using AU5800 fully automated biochemical analyzer corresponding commercial kit. The Kolmogorov-Smirnov test used analyze activity. Following guidelines C28-A3 WS/T 402-2024, interval determined bilateral percentile method, covering range 2.5th 97.5th percentile. Results exhibited non-normal distribution. Significant gender-based differences were observed, while no significant variations found across different age groups. Based on these findings, gender-specific established: 26.6–51.8 U/l males 29.7–55.3 females. Conclusions first adults. These provide essential guidance health screening improve diagnosis management related clinical conditions.

Language: Английский

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A novel fluorescent sensing platform for glutathione based on Förster resonance energy transfer and aggregation-induced emission

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Journal Year: 2025, Volume and Issue: unknown, P. 126131 - 126131

Published: March 1, 2025

Language: Английский

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Enhancing glutathione delivery and efficacy: a novel microcapsule approach

Journal of Materials Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 15, 2025

Language: Английский

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Genome-Based in silico Evaluation of the Biosafety and Probiotic Traits of Limosilactobacillus fermentum J23 Isolated from Artisanal Mexican Cheese

Food Biotechnology, Journal Year: 2024, Volume and Issue: 38(1), P. 1 - 29

Published: Jan. 2, 2024

Comprehensive in silico analysis aimed to identify the genes involved biosafety and probiotic features of Limosilactobacillus fermentum J23 was performed. The strain had a single circular chromosome 2,004,882 base pairs (bp), with Guanine + Cytosine (G C) content 51.74%. contained 2,001 predicted as protein-coding sequences (CDS), 86 pseudogenes, 56 tRNAs, five rRNAs, three ncRNAs. demonstrated absence risk-related corresponding transferable/acquirable antibiotic resistance, virulence factors, toxic metabolites, detrimental metabolic/enzymatic activities. nonpathogenic humans presence variety potentially related its profile were revealed, including those carbohydrate metabolism transport, adhesion aggregation ability, stress/adaptive response, CRISPR/Cas adaptive immunity, immunomodulation. These findings highlight potential L. provide genomic clues advance future applications.

Language: Английский

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African swine fever virus enhances viral replication by increasing intracellular reduced glutathione levels, which suppresses stress granule formation

Veterinary Research, Journal Year: 2024, Volume and Issue: 55(1)

Published: Dec. 20, 2024

Abstract African swine fever virus (ASFV) is a DNA that has significantly impacted the global industry. Currently, there are no effective therapies or vaccines against ASFV. Stress granules (SGs), known for their antiviral properties, not induced during ASFV infection, even though reactive oxygen species (ROS) generated. The mechanism by which regulates SGs formation remains unclear. This study demonstrates antagonises and increases intracellular levels of reduced glutathione (GSH) levels. use GSH inhibitor BSO activator NAC confirmed ASFV-induced increase in helps to suppress influences viral replication. Additionally, this revealed enhances upregulating antioxidant transcription factor NRF2, as well factors involved synthesis regeneration, such GCLC, those related ferroptosis pathway, SLC7A11. Furthermore, uncovered manipulates activating mitochondrial protein AIFM1. regulatory inhibit SGs, thereby creating an optimal environment These findings provide new insights into molecular strategies employed

Language: Английский

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Poly(Vinyl Alcohol)/Bovine Serum Albumin Hybrid Hydrogels with Tunable Mechanical Properties

Polymers, Journal Year: 2023, Volume and Issue: 15(23), P. 4611 - 4611

Published: Dec. 4, 2023

In this study, a new strategy was adopted for obtaining polymer/protein hybrid hydrogels with shape stability and tunable mechanical or rheological characteristics by using non-toxic procedures. A chemical network created poly(vinyl alcohol)(PVA)/bovine serum albumin (BSA) mixture in aqueous solution the presence of genipin reduced glutathione (GSH). Then, second physical formed through PVA after applying freezing/thawing cycles. addition, protein macromolecules intermolecular disulfide bridges GSH. these conditions, multiple crosslinked networks were obtained, determining strengthening stiffening into relatively tough porous viscoelasticity self-healing ability. SEM analysis evidenced formation interconnected pores sizes between 20 μm 50 μm. The investigations showed that hydrogels’ strength response different conditions deformation influenced composition crosslinking procedure. Thus, dynamics can be adjusted to mimic viscoelastic properties native tissues. dynamic water vapor-sorption ability, swelling behavior an environment, bioadhesive also investigated are discussed paper. designed on request, they promising candidates tissue engineering, bioinks, wound dressing applications.

Language: Английский

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Network-based Multi-omics Disease–Drug Associations Reveal Drug Repurposing Candidates for Covid-19 Disease Phases

Drug repurposing, Journal Year: 2024, Volume and Issue: 1(1)

Published: July 3, 2024

Background The development and rollout of vaccines the use various drugs have contributed to controlling coronavirus disease 2019 (Covid-19) pandemic. Nevertheless, challenges such as inequitable distribution vaccines, influence emerging viral lineages immunoevasive variants on vaccine efficacy, inadequate immune defense in subgroups population continue motivate new combat disease. Aim In this study, we sought identify, prioritize, characterize drug repurposing candidates appropriate for treating mild, moderate, or severe Covid-19 using a network-based integrative approach that systematically integrates drug-related data multi-omics datasets. Methods We leveraged used random walk with restart algorithm explore an integrated knowledge graph comprising three subgraphs: (i) graph, (ii) (iii) state-specific omics graph. Results prioritized 20 US Food Drug Administration-approved agents potential candidate phases. Specifically, could stimulate cell recruitment activation including histamine, curcumin, paclitaxel utility mild states mitigate progression. Drugs like omacetaxine, crizotinib, vorinostat exhibit antiviral properties inhibit replication can be considered moderate states. Also, given association between antioxidant deficiency high inflammatory factors trigger cytokine storms, antioxidants glutathione potent anti-inflammatory effects (sarilumab tocilizumab), corticosteroids (dexamethasone hydrocortisone), immunosuppressives (sirolimus cyclosporine) are hyperinflammatory cascade Covid-19. Conclusion Our study demonstrates data-driven analysis within enables prioritizing phases, offering comprehensive basis therapeutic strategies brought market quickly their established safety profiles. Importantly, implemented here prioritize other diseases.

Language: Английский

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