Disrupting circadian control of autophagy induces podocyte injury and proteinuria

Kidney International, Год журнала: 2024, Номер 105(5), С. 1020 - 1034

Опубликована: Фев. 21, 2024

The circadian clock influences a wide range of biological process and controls numerous aspects physiology to adapt the daily environmental changes caused by Earth's rotation. kidney plays an important role in maintaining tubular function, but its effect on podocytes remains unclear. Here, we found that expressed CLOCK proteins, 2666 glomerular gene transcripts (13.4%), including autophagy related genes, had 24-hour rhythms. Deletion Clock resulted 1666 with loss rhythm genes. Podocyte-specific knockout mice at age three eight months showed deficient autophagy, increased albuminuria. Chromatin immunoprecipitation (ChIP) sequence analysis indicated genes were targets podocytes. ChIP-PCR further confirmed binding promoter regions Becn1 Atg12, two Furthermore, association regulated chronic sleep fragmentation diabetic disease was analyzed. Chronic rhythm, inhibition podocyte proteinuria. Rhythmic oscillations also disappeared high glucose treated glomeruli from mice. Finally, differences abolished aggravated injury proteinuria Thus, our findings demonstrate clock-dependent regulation may be essential for survival. Hence. controlled play

Язык: Английский

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Sleep and Oxidative Stress: Current Perspectives on the Role of NRF2

Cellular and Molecular Neurobiology, Год журнала: 2024, Номер 44(1)

Опубликована: Июнь 25, 2024

Abstract Sleep is a fundamental conserved physiological state across evolution, suggesting vital biological functions that are yet to be fully clarified. However, our understanding of the neural and molecular basis sleep regulation has increased rapidly in recent years. Among various processes implicated controlling homeostasis, bidirectional relationship between oxidative stress recently emerged. One proposed function may mitigation both brain peripheral tissues, contributing clearance reactive species accumulate during wakefulness. Conversely, species, such as oxygen (ROS) nitrogen (RNS), at levels, act signaling agents regulate redox-sensitive transcriptional factors, enzymes, other effectors involved sleep. As primary sensor intracellular oxidation, transcription factor NRF2 emerging an indispensable component maintain cellular redox homeostasis Indeed, number studies have revealed association dysfunction most common conditions, including loss, obstructive apnea, circadian disturbances. This review examines evidence intricate link context sleep, highlights potential modulators alleviate Graphical A been shown, indicating play protective role against accumulation wakefulness deprivation. might also serve molecules influence mechanisms. Notably, changes, key regulator homeostasis.

Язык: Английский

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Melatonin as a Mediator of the Gut Microbiota–Host Interaction: Implications for Health and Disease

Antioxidants, Год журнала: 2023, Номер 13(1), С. 34 - 34

Опубликована: Дек. 23, 2023

In recent years, the role played by melatonin on gut microbiota has gained increasingly greater attention. Additionally, been proposed as an alternative source of melatonin, suggesting that this antioxidant indoleamine could act a sort messenger between and host. This review analyses available scientific literature about possible mechanisms involved in mediating role, highlighting its effects influence interaction. addition, we also knowledge composition, well ability to alleviate dysbiosis related sleep deprivation or chronodisruptive conditions. The melatonin–gut relationship discussed terms development different disorders, from inflammatory metabolic disorders psychiatric neurological conditions, considering oxidative stress reactive oxygen species-scavenging properties main factors relationship.

Язык: Английский

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Improving Sleep Quality and Well-Being in Institutionalized Older Adults: The Potential of NESA Non-Invasive Neuromodulation Treatment

Geriatrics, Год журнала: 2025, Номер 10(1), С. 4 - 4

Опубликована: Янв. 3, 2025

Background/Objectives: Ageing is associated with several cognitive, physical, and emotional changes, including a decrease in sleep quality mental health issues. This study studies NESA (Spanish acronym for Neuromodulación Superficial Aplicada) non-invasive neuromodulation using microcurrents as something that may provide potential improvement the of general older adults residents healthcare institution. Methods: observational recruited 24 people who were at long-term care facility. Participants divided into two groups: one intervention group, underwent therapy twice week total 20 sessions, control did not receive this treatment. The outcomes measured include (Pittsburgh Sleep Quality Index), diary sleep, symptoms depressed mood (Yesavage Geriatric Depression Scale), life World Health Organization Life-Old (WHOQOL-OLD). Scores collected baseline, after 10 3 months finishing Results: group exhibited notable (p = 0.05). Additionally, there fewer nocturnal awakenings. also showed better scores, especially relation to social relations physical health, which matches slight scores clinical regarding depressive symptoms. In contrast, demonstrated no symptoms, some cases, was worsening Conclusions: Our findings indicate likely enhance health-related measures institutionalized adults. Despite limitations current study, results support well-being population.

Язык: Английский

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The Extract of Piper nigrum Improves the Cognitive Impairment and Mood in Sleep-Deprived Mice Through the JAK1/STAT3 Signalling Pathway

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(5), С. 1842 - 1842

Опубликована: Фев. 21, 2025

Piper nigrum L. (PN), which contains various bioactive compounds, is a plant with homologous medicine and food. Sleep deprivation (SD) profoundly impacts cognitive function emotional health. However, the mechanisms by PN improves depressive mood induced SD remain unclear. In our study, network pharmacology molecular docking techniques were used to predict potential regulates SD. this 220 compounds identified in PN, 10 core targets screened through pharmacology. Animal experiments showed that ameliorated deficits sleep-deprived mice, upregulated serum activities of superoxide dismutase (SOD), glutathione (GSH), catalase (CAT), downregulated malondialdehyde (MDA) levels. The ELISA assay significantly decreased tumour necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), interleukin-1 beta (IL-1β) Histopathological staining brain tissue demonstrated mitigates SD-induced hippocampal damage, enables hippocampus produce more neurotransmitters, including 5-hydroxytryptamine (5-HT), gamma-aminobutyric acid (GABA), dopamine (DA), reduces glutamate (Glu) RT-qPCR WB analyses further indicated could exert anti-SD effects inhibiting over-activation JAK1/STAT3 signalling pathway. PC12 cell model, reduce inflammation prevent apoptosis, exerting neuroprotective effects. summary, has positive on alleviating symptoms dysfunction

Язык: Английский

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Daily physical activity, coffee and energy drink consumption, and sleep patterns among Chinese elite professional esports athletes: a case study of Zhejiang Regans Gaming

Frontiers in Public Health, Год журнала: 2025, Номер 13

Опубликована: Фев. 26, 2025

Esports is increasingly recognized as both a legitimate sport and profession. However, evidence on the health behaviors of esports athletes remains limited. Due to nature their activity, these face occupational risks associated with prolonged sedentary behavior, sleep deprivation, excessive energy drink consumption. This study aimed document professional athletes. case sampled from Zhejiang Regans Gaming, who are full-time professionals competing at elite level. Data were collected over three training days, moderate-to-vigorous physical activity patterns monitored using Apple Watch Series 9. Athletes trained for an average 13 h daily in seated position, engaging only 35.6 ± 0.9 min activity. The extended hours led delayed schedules, typical bedtime 3:05 a.m. Sleep quality was poor, averaging 45.2 13.0 awake bed, 392.8 13.9 total sleep, efficiency 89.7 3.0%. Likely due consumed 2 cups coffee 2.5 0.4 bottles drinks daily. offers firsthand concerning among Chinese Physical levels notably while consumption prevalent. Team managers should monitor consumption, governing bodies must recognize long-term behavior esports.

Язык: Английский

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Lipidome Alterations of Prefrontal Cortex and Hippocampus of Mice‐Induced Sleep Deprivation

ChemistrySelect, Год журнала: 2025, Номер 10(8)

Опубликована: Фев. 1, 2025

Abstract Chronic sleep deprivation (SD) impairscognitive function and promotes neurodegenerative changes, particularly in thehippocampus prefrontal cortex. Study investigates the biochemical andimmunological effects of SD evaluates neuroprotective properties ofmelatonin using Fourier Transform Raman (FT‐Raman) spectroscopy. Hippocampaland cortical tissues from four groups micecontrol, melatonin‐treated, sleep‐deprived, sleep‐deprived with melatoninwere analyzed. FT‐Ramanspectra revealed significant changes C─H bending, C─C vibrations, amides, lipids between control groups. led to increased lipidperoxidation, especially hippocampus, while melatonin treatment reducedlipid peroxidation amide levels. In cortex, mitigatedbiochemical alterations associated SD, such as elevated amides lipids.Immunological assessments showed SD‐induced T helper (Th) cellsubpopulations, including an increase effector Th cells a decrease innaive cells. Melatonin normalized these changes. PrincipalComponent Analysis (PCA) indicated clear separation SDsamples, PC1 PC2 explaining 95.58% variance. Classificationusing GS‐kNN algorithm achieved cortex (accuracies: 79.167%) andin hippocampus 70.833%). These findings highlight SD‐inducedbiochemical immunological suggest melatonin'sneuroprotective role reducing oxidative stress modulating immunedysfunction.

Язык: Английский

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Altered brain-derived neurotrophic factor levels and oxidative stress in REM sleep deprivation: a rat model study

BMC Neurology, Год журнала: 2025, Номер 25(1)

Опубликована: Март 21, 2025

Brain-derived neurotrophic factor (BDNF) is among the modulators associated with cognition and sleep that play a role in disorders. This study aimed at investigating effects of chronic deprivation REM on BDNF levels oxidative stress markers. A total 24 healthy male Wistar albino rats were separated into 3 groups as group, control group group. To create models 21-day deprivation, we used platform technique. After 21 days blood BDNF, brain tissue malondialdehyde, glutathione, ascorbic acid, nitrite nitrate evaluated. Compared showed significant increase (p = 0.038), whereas decrease was observed glutathione (GSH) nitric oxide (NO) (p:0.036). No statistical difference between either (p: 0.795). Our results decreases GSH NO increases malondialdehyde models, reflecting brain. Additionally, model.

Язык: Английский

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Caffeine and Exercise: A Dual Approach to Combat Cognitive Decline Induced by REM Sleep Deprivation

Molecular Neurobiology, Год журнала: 2025, Номер unknown

Опубликована: Март 25, 2025

Язык: Английский

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The protective effect of Bromelain on Testicular Dysfunction and Oxidative Stress Induced by REM Sleep Deprivation in Adult Male Rats

Tissue and Cell, Год журнала: 2025, Номер unknown, С. 102927 - 102927

Опубликована: Апрель 1, 2025

Язык: Английский

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