Green biosynthesis of titanium dioxide quantum dots using watermelon peel waste: antimicrobial, antioxidant, and anticancer activities

Biomass Conversion and Biorefinery, Год журнала: 2022, Номер 14(5), С. 6987 - 6998

Опубликована: Май 16, 2022

Язык: Английский

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In vitro assessment of multipotential therapeutic importance of Hericium erinaceus mushroom extracts using different solvents

Bioresources and Bioprocessing, Год журнала: 2022, Номер 9(1)

Опубликована: Сен. 16, 2022

Abstract Infectious disease is one of the major threats to humans and it second leading cause death worldwide. Edible mushrooms have many nutritional medicinal values human health. The properties edible mushroom extract in inhibiting pathogenic microorganisms had advantages over use chemically synthetic antimicrobial compounds due less unwanted side effects can combat microbial resistance. This study hypothesized that polarity affects extraction quality Hericium erinaceus fruiting bodies which was prepared subsequently its activity as an against six tested microorganisms, including MRSA, Streptococcus mutans , Enterobacter cloaca Salmonella typhimurium Candida lipolytica ; antiviral Hepatitis A virus (HAV) virus; antioxidant using 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay; anti-inflammatory potential. So, quantitatively evaluated assess content flavonoids, alkaloids, tannins, saponins, carbohydrates, protein, nitrogen, oil. current research clarified here aquatic has a significant (IC 50 = 53.7 µg/mL) 24.97 µg/mL), while ethyl acetate showed reasonable rather than all microorganisms. Unfortunately, extracts under investigation possess low action according adopting protocol. superior results both water were later investigated by HPTLC GC–MS for preliminary prediction chemical constitution those extracts. H. succeeded establish promising antimicrobial, antiviral, activities activity. Both could identify constituents crude extract. Graphical

Язык: Английский

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Searching for new antifungals for the treatment of cryptococcosis

Revista da Sociedade Brasileira de Medicina Tropical, Год журнала: 2023, Номер 56

Опубликована: Янв. 1, 2023

There is a consensus that the antifungal repertoire for treatment of cryptococcal infections limited. Standard involves administration an drug derived from natural sources (i.e., amphotericin B) and two other drugs developed synthetically flucytosine fluconazole). Despite treatment, mortality rates associated with fungal cryptococcosis are high. Amphotericin B toxic, require intravenous administration, usually unavailable in low-income countries because their high cost. However, fluconazole cost-effective, widely available, harmless regard to its side effects. fungistatic agent has contributed considerably increase resistance frequent relapses patients meningitis. Therefore, there unquestionable need identify new alternatives or adjuvants conventional cryptococcosis. A potential should be able kill cryptococci "bypass" virulence mechanism yeast. Furthermore, it have fungicidal action, low toxicity, selectivity, easily penetrate central nervous system, available. In this review, we describe cryptococcosis, therapy, failures arising use traditionally considered reference standard. Additionally, present approaches used discovery counteract ranging screening products inclusion structural modifications optimize anticryptococcal activity, as well repositioning combined therapies.

Язык: Английский

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Promising sponge-derived marine fungi as antibacterial and biofilm inhibitors

Journal of Applied Pharmaceutical Science, Год журнала: 2024, Номер unknown

Опубликована: Янв. 1, 2024

Marine organisms, especially sponges, provide many sources of metabolites with various biological activities. Most sponges associate microbes such as fungi. To solve the problem sponge availability, it is necessary to isolate compounds from marine-derived fungi due their feasibility and advantages. This study, thus, highlights that most prominent genera produce active antibacterials antibiofilm were Aspergillus, Penicillium, Neosartorya, Trichoderma. The summarized data isolated related antibacterial activities until 2022 included anthraquinones, sterigmatocystin analogs, hydroxy pyrrolidine alkaloids, helvolic acid derivatives, lactones, prenylated phenylbutyrolactones, citrinin bisthiodiketopiperazine, cyclotetrapeptides, dihydrochromone dimers, amino lipopeptides, furan aspiron-derivatives, halogenated metabolites, alkaloids. Since biofilm mechanism very complex, some do not necessarily work antibiofilms. Nevertheless, can be concluded produced sponge-associated have potential developed new agents although still require further investigation actions compounds.

Язык: Английский

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Exploring the Metabolic Profiling and Anticancer Activities of Red Sea Sponges Echinodictyum asperum and Callyspongia siphonella against Human Breast Cancer Cells: A Comparative Study

ACS Omega, Год журнала: 2025, Номер 10(9), С. 9402 - 9425

Опубликована: Фев. 25, 2025

Marine sponges are noteworthy sources of bioactive secondary metabolites that demonstrate fundamental functions in defense mechanisms and ecological interactions. This study focused on the extraction characterization from two sponge species, Echinodictyum asperum Callyspongia siphonella, obtained Red Sea coast Saudi Arabia. Methanol generated EAE CSE extracts, which were subjected to metabolic profiling, illuminating 44 identified involving fatty acids, sterols, terpenoids, long-chain amides, alkaloids. The cytotoxic effects both assessed versus MDA-MB-231 MCF-7 breast cancer cell lines, utilizing sulforhodamine B (SRB) assay, with IC50 values about 2.7 μg/mL for 0.5 cells 8.7 45.3 cells. Flow cytometry analysis indicated extracts induced apoptosis necrosis across lines. Particularly, G2 phase arrest cells, while elevated S population As well, boosted autophagy especially Comparative analyses existing literature underscore therapeutic potential these sponge-derived medication. Molecular docking studies demonstrated robust binding affinities between selected key targets MCL-1, BCL-2, ERα. In conclusion, this research emphasizes significant features proposing their purposes treatment via distinct action.

Язык: Английский

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Whole genome sequencing of rice endophyte Bacillus paralicheniformis NB stem 4: A potential biocontrol agent for the suppression of pearl millet blast disease

Physiological and Molecular Plant Pathology, Год журнала: 2025, Номер unknown, С. 102663 - 102663

Опубликована: Март 1, 2025

Язык: Английский

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Antineoplastic with DNA fragmentation assay and anti-oxidant, anti-inflammatory with gene expression activity of Lactobacillus plantarum isolated from local Egyptian milk products

BMC Microbiology, Год журнала: 2024, Номер 24(1)

Опубликована: Окт. 29, 2024

Abstract Many lactic acid bacteria (LAB), known for their human health benefits, are derived from milk and utilized in biotherapeutic applications or producing valuable nutraceuticals. However, the specific role of milk-associated LAB biotherapeutics remains underexplored. To address this, eight product samples were randomly selected Egyptian market, diluted, then cultured anaerobically on MRS agar. Subsequently, 16 suspected isolates recovered underwent rapid preliminary identification. Among these isolates, Lactobacillus plantarum strain with accession number (OQ547261.1) was identified due to its strong antioxidant activity depending DPPH assay, L. displayed notable activities 71.8% 93.8% at concentrations 125–1000 µg/mL, respectively. While ascorbic showed lower 7.81, 3.9, 1.95 µg/mL which 45.1%, 34.2%, 27.2%, The anti-inflammatory efficacy evaluated based capability prevent hemolysis induced by hypotonic conditions. At a concentration 1000 could reduce 97.7%, nearly matching 99.5% inhibition rate achieved standard drug, indomethacin, an identical concentration. Moreover, exhibited high hemolytic 100 (14.3%), decreased 1.4% µg/mL. abundance phenolic acids flavonoids determined high-performance liquid chromatography (HPLC) . Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) demonstrated that increased gene expression inflammatory marker TLR2 133%, cellular oxidation markers SOD1 SOD2 65% 74.2%, respectively, while suppressing CRP 33.3%. These results underscore plantarum’s exceptional activities. Furthermore, induces cancer cell death through necrotic nuclear DNA fragmentation. findings suggest is not only suitable nutraceutical production but also holds potential as probiotic strain. Future research should focus enhancing capacity this across various industries fostering innovation multiple fields.

Язык: Английский

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Biosynthesized ZnO-NPs Using Sea Cucumber (Holothuria impatiens): Antimicrobial Potential, Insecticidal Activity and In Vivo Toxicity in Nile Tilapia Fish, Oreochromis niloticus

Separations, Год журнала: 2023, Номер 10(3), С. 173 - 173

Опубликована: Март 3, 2023

In this study, a sustainable and eco-friendly method was used to prepare zinc oxide nanoparticles (ZnO-NPs) using sea cucumber aqueous extract. Then, ZnO-NPs were characterized by instrumental analysis (UV-vis, HR-TEM, XRD, FT-IR, DLS) evaluated for their possible antibacterial, antifungal, insecticidal activities. Additionally, the toxicity of in vivo against Nile Tilapia (Oreochromis niloticus). The collected from Gulf Suez (Red Sea) at Al-Ain Al-Sokhna coast Egypt identified as Holothuria impatiens. prepared Hi-ZnO-NPs peaked 350 nm UV–Vis spectral analysis. They showed quasi-spherical shaped particles with sizes ranging 13 47 predominate size 26 indicated HR-TEM. XRD pattern revealed crystalline phase an average 17.2 calculated Debye–Scherrer equation. FTIR role H. impatiens biological molecules biosynthesis process ZnO-NPs. negative zeta potential −19.6 mV, demonstrating moderate stability. Biosynthesized broad antimicrobial activity Gram-positive bacteria (S. aureus ATCC 25923 E. feacalis), Gram-negative typhi, K. pneumonia coli), filamentous fungi (Aspergillus niger). demonstrated larvicidal mosquito, Culex pipiens (LC50 = 2.756 ppm LC90 9.294 ppm), adulticidal action housefly, Musca domestica (LD50 4.285 LD90 22.847 ppm). Interestingly, did not show mortality effects tilapia fish niloticus), highlighting safety highly exposed, non-target organisms. However, histopathological hematological investigations provided dose-dependent impacts tilapia. Overall, data provide approach synthesizing novel multiple biomedical properties potentially low fish.

Язык: Английский

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A multi-aspect analysis of two analogous aspergillus spp. belonging to section Flavi: aspergillus flavus and aspergillus oryzae

BMC Microbiology, Год журнала: 2023, Номер 23(1)

Опубликована: Март 15, 2023

Microfungal isolates were routinely identified depending on both macro and micro morphological characteristics, sometimes, some fungal appeared to be similar such cases caused severe confusion for mycologists during the preliminary identification. During our previous studies dealing with isolation of fungi biotechnological applications; two mystifying species Aspergillus flavus oryzae showed cultural macroscopic features. Therefore, current study aimed easily distinguish between these simple approaches which are followed by a large segment researchers. Investigation features was performed check growth four different media (PDA, MEA, YES, CYA) microscopic examination using an ordinary light microscope, scanning electron microscope SEM. Also, screening secondary metabolites strains preliminarily find out difference their metabolic profiles. Finally, ITS rDNA involved clarify molecular differences along partial sequence. Conclusively, BLAST strategy confirmed similarity segments that supported hypothesis. The color is very critical factor whereas it extensively influenced type cultivation media. Accordingly, YES medium inspiring tool assisting in prompt differentiation culture investigation step A. significant mustard yellow olive green respectively. examination, CYA also had robust effect formation conidial chain knit long observed while conidia scattered not case flavus. Likewise, possessed profiles where Afla toxin producer, unlike

Язык: Английский

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Potential carcinogenic and non-carcinogenic health risks of heavy metals ingestion from consumption of the crayfish, Procambarus clarkii in El-Rahawy Drain and El-Kanater in the River Nile, Egypt

Egyptian Journal of Aquatic Biology and Fisheries, Год журнала: 2022, Номер 26(3), С. 667 - 686

Опубликована: Май 1, 2022

Heavy metals can constitute a critical risk to freshwater fauna via exposure, bioaccumulation, and biomagnification processes. The results confirmed that heavy in the water, sediments, tissues of crayfish were considerably higher El-Rahawy sites comparison El-Kanater (P<0.05). Fe levels most abundant concentration compared remaining (Co, Zn, As, Cd, Pb) sediment, at all sites. Regarding Hms tissues, bioaccumulation sequence HMs was decreasing exoskeleton > gills hepatopancreas muscles for Co, Pb, Hepatopancreas Exoskeleton As. HI values muscles, people who eat normal amount have no non-carcinogenic risk. On other hand, calculated habitual eaters than one which indicates potential contract, As Cd pose carcinogenic consuming muscular should be considered hazard message based on data indices human health viewpoint. Accordingly, current study recommends government environmental management Egypt conduct routine HM monitoring drain order lessen risks.

Язык: Английский

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