Fertility-Sparing Strategies for Early-Stage Endometrial Cancer: Stepping towards Precision Medicine Based on the Molecular Fingerprint

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(1), С. 811 - 811

Опубликована: Янв. 3, 2023

Endometrial cancer represents the fifth most common in women, and gynecological malignancy developed countries [...].

Язык: Английский

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Stem Cells and Infertility: A Review of Clinical Applications and Legal Frameworks

Journal of Personalized Medicine, Год журнала: 2024, Номер 14(2), С. 135 - 135

Опубликована: Янв. 24, 2024

Infertility is a condition defined by the failure to establish clinical pregnancy after 12 months of regular, unprotected sexual intercourse or due an impairment person’s capacity reproduce either as individual with their partner. The authors have set out succinctly investigate, explore, and assess infertility treatments, harnessing potential stem cells effectively safely treat infertility; in addition, this paper will present legal regulatory complexities at heart cell research, overview legislative state affairs six major European countries. For couples who cannot benefit from assisted reproductive technologies (ART) infertility, stem-cells-based approaches been shown be highly promising approach. Nonetheless, lingering ethical immunological uncertainties require more conclusive findings data before such treatment avenues can become mainstream applied on large scale. isolation human embryonic (ESCs) ethically controversial, since collection involves destruction tissue. Overall, research has resulted important new breakthroughs infertility. effort untangle complex web issues associated therapeutic rely evidence-based, broadly shared standards, guidelines, best practices make sure that procreative rights patients reconciled core values medical ethics.

Язык: Английский

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Deep learning paradigms in lung cancer diagnosis: A methodological review, open challenges, and future directions

Physica Medica, Год журнала: 2025, Номер 131, С. 104914 - 104914

Опубликована: Фев. 11, 2025

Язык: Английский

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Synchronous Endometrial and Ovarian Adenocarcinomas in a 43-Year-Old Patient Following Infertility Treatment: A Case Report

Diagnostics, Год журнала: 2025, Номер 15(6), С. 670 - 670

Опубликована: Март 10, 2025

Background and Clinical Significance: This study presents a case of 43-year-old female with long history infertility, treated for uterine leiomyoma endometrial hyperplasia, over total observation period 42 months. Case Presentation: Levonorgestrel intrauterine device (LNG-IUD) therapy, as first subsequent line treatment, was introduced. The patient also received medroxyprogesterone acetate oral treatment. Finally, she underwent surgery an ovarian tumor that appeared to be adenocarcinoma concurrent cancer. After the removal reproductive organ, diagnosed synchronous low-grade endometrioid in endometrium grade 2 (G2) left ovary. Conclusions: prognosis further management largely depend on whether these are two individual neoplasms or one metastatic tumor. Considering young age patients, early disease stage, low both cancers, favorable prognosis, most cancers identified independent primary tumors. diagnosis multi-focal neoplasm is important, patients cancer metastasis, 5-year survival rate 30–40%, whereas neoplasms, it 75–80%.

Язык: Английский

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MTFR2 promotes endometrial carcinoma cell proliferation and growth via the miR-132-3p/PI3K/Akt signaling pathway

Frontiers in Medicine, Год журнала: 2025, Номер 11

Опубликована: Март 10, 2025

Objective Understanding the mechanisms underlying endometrial cancer progression is crucial for development of effective targeted therapies. In this study, we investigated role MTFR2 in cell. Methods The expression was analyzed using Cancer Genome Atlas (TCGA) dataset and detected tissues cells, respectively. Gain-of-function loss-of-function approaches were utilized to investigate impact on cell proliferation tumorigenesis both vitro vivo settings. Computational tools employed predict microRNAs (miRNAs) that potentially regulate MTFR2, these predictions experimentally validated. Results enhanced carcinoma, it positively correlated with poor prognosis patients. Functional studies show promoted proliferation, migration invasion cells. Bioinformatics analysis luciferase assays identified a potential target miR-132-3p, transfection miR-132-3p mimics attenuated MTFR2-induced activation PI3K/Akt pathway. Conclusion Our findings highlight critical promoting growth through miR-132-3p/PI3K/Akt signaling Targeting axis may offer therapeutic strategies treatment.

Язык: Английский

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Circulating miRNAs as a Tool for Early Diagnosis of Endometrial Cancer—Implications for the Fertility-Sparing Process: Clinical, Biological, and Legal Aspects

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(14), С. 11356 - 11356

Опубликована: Июль 12, 2023

This review article explores the possibility of developing an integrated approach to management different needs endometrial cancer (EC) patients seeking become pregnant. Life preservation woman, health baby, a precocious and—as much as possible—minimally invasive characterization and fertility parameters patient, together with concerns regarding obstetric, neonatal, adult risks children conceived via assisted reproductive techniques (ART) are all essential aspects problem be taken into consideration, yet harmonize such through concerted is still very challenging. aims illustrate main features EC how it affects normal physiology pre-menopausal women. We also focus on prospect miR-based, molecular evaluation patient status, including both early diagnosis staging and, similarly, receptivity discussing possible using single specific panel circulating miRs in thus allowing relatively fast, non-invasive testing significantly reduced margin error. Finally, ethical legal/regulatory innovative require not only risk-benefit analysis; respect for autonomy equitable care access allocation fundamental issues well.

Язык: Английский

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Navigating Fertility Preservation Options in Gynecological Cancers: A Comprehensive Review

Cancers, Год журнала: 2024, Номер 16(12), С. 2214 - 2214

Опубликована: Июнь 13, 2024

(1) Background: Currently, an increasing number of women postpone pregnancy beyond the age 35. Gynecological cancers affect a significant proportion reproductive age, necessitating development fertility preservation methods to fulfill family planning. Consequently, providing treatment options that preserve in diagnosed with gynecological has become crucial component care for survivors. (2) Methods: We conducted extensive search relevant scientific publications PubMed and Embase databases performed narrative review, including high-quality peer-reviewed research on after being treated gynecologic cancers, reporting rates, birth outcomes cancer survivors as well therapeutic which partially obtaining (3) Discussion: The medicine practiced today is focused both treating neoplasm preserving quality life patients, important element this quality. This leads improved life, allowing these mothers even seemingly adverse circumstances posed by such pathology. However, although there are guidelines female context neoplasms, analysis shows physicians do not routinely consider it discuss their patients. (4) Conclusions: Advancements have led better understanding management resulting increased survival rates. Once battle against neoplasms won, issue arises, aspect who yet fulfilled planning desires at time diagnosis. It patients be informed about available encouraged make decisions collaboration medical team. Standardized recommendations onco-fertility into should taken consideration future.

Язык: Английский

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Circulating miRNAs as a Tool for Early Diagnosis of Endometrial Cancer – Implications for the Fertility-sparing Process: Clinical, Biological and Legal Aspects

Опубликована: Июнь 6, 2023

This review article is aimed at exploring the possibility of developing an integrated approach in management different needs endometrial cancer (EC) patients seeking to become pregnant. Life preservation woman, health baby, a precocious and – as much possible minimally invasive characterization fertility parameters patient, together with concerns regarding obstetric, neonatal, adult risks children conceived via assisted reproduction techniques (ART) are all essential aspects problem be taken into consideration, yet harmonize such through concerted still highly challenging. aims illustrate main features EC how it affects normal physiology pre-menopausal women. Then, we focus on prospect for miR-based, molecular evaluation patient status, including both early diagnosis staging and, similarly, receptivity discussing using single specific panel circulating miR thus allowing relatively fast, non-invasive way perform testing significantly reduced margin error. Finally, ethical legal/regulatory complexities arising from innovative require not only risk-benefit analysis; respect autonomy equitable care access allocation fundamental issues well.

Язык: Английский

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Inflammatory Cytokine-Induced HIF-1 Activation Promotes Epithelial–Mesenchymal Transition in Endometrial Epithelial Cells

Biomedicines, Год журнала: 2023, Номер 11(1), С. 210 - 210

Опубликована: Янв. 14, 2023

The endometrium undergoes repeated proliferation and shedding during the menstrual cycle. Significant changes to this environment include fluctuations in partial pressure of oxygen, exposure a high-cytokine associated with intrauterine infection, inflammation. Chronic endometritis is condition wherein mild inflammation persists one causes implantation failure miscarriage early pregnancy. It thought that invasion embryos into requires epithelial-mesenchymal transition (EMT)-associated endometrial epithelium. However, effects on remain poorly understood. In study, we investigated oxygen environment, hypoxia-inducible factor (HIF), differentiation function epithelial cells. We elucidated ways which inflammatory cytokines affect HIF activity EMT an immortalized cell line (EM-E6/E7/TERT) derived from Pro-inflammatory caused significant accumulation HIF-1α protein, increased mRNA levels, enhanced hypoxia-induced protein. combined effect hypoxia expression EMT-inducing factors upregulated migration. Our findings indicate pro-inflammatory factors, including LPS, work synergistically activate HIF-1 promote

Язык: Английский

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Which endometrial preparation protocol provides better pregnancy and perinatal outcomes for endometriosis patients in frozen-thawed embryo transfer cycles? A retrospective study on 1413 patients

Journal of Ovarian Research, Год журнала: 2023, Номер 16(1)

Опубликована: Янв. 9, 2023

To determine the optimal endometrial preparation protocol for a frozen embryo transfer in patients with endometriosis. Retrospective cohort study. Tertiary care academic medical center. One thousand four hundred thirteen endometriosis who underwent oocyte aspiration from 2015 to 2020 and 2016 received natural cycle, hormone replacement treatment or without GnRHa pretreatment preparation. None. Clinical pregnancy rate, live birth miscarriage multiple biochemical rate ectopic rate. Singleton births were assessed perinatal outcomes obstetric complications. There no differences clinical prenatal among three commonly used protocols cycles Results remained after screening variables using univariate logistic regression into multivariate regression. No advantages disadvantages found Natural showed superiority inferiority

Язык: Английский

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