Molecular and Cellular Biochemistry, Год журнала: 2022, Номер 478(4), С. 721 - 727
Опубликована: Сен. 2, 2022
Язык: Английский
Pharmaceuticals, Год журнала: 2023, Номер 16(3), С. 474 - 474
Опубликована: Март 22, 2023
Sufficient ocular bioavailability is often considered a challenge by the researchers, due to complex structure of eye and its protective physiological mechanisms. In addition, low viscosity drops resulting short residence time further contribute observed drug concentration at target site. Therefore, various delivery platforms are being developed enhance bioavailability, provide controlled sustained release, reduce number applications, maximize therapy outcomes. Solid lipid nanoparticles (SLNs) nanostructured carriers (NLCs) exhibit all these benefits, in addition biocompatible, biodegradable, susceptible sterilization scale-up. Furthermore, their successive surface modification contributes prolonged (by adding cationic compounds), enhanced penetration, improved performance. The review highlights salient characteristics SLNs NLCs concerning delivery, updates research progress this area.
Язык: Английский
Процитировано
29
Smart Molecules, Год журнала: 2024, Номер 2(1)
Опубликована: Март 1, 2024
Abstract This comprehensive review delves into a unique intersection of hydrogels as smart molecules and their transformative applications in ophthalmology. Beginning with the foundational definition, properties, classification hydrogels, explores synthesis responsive capabilities. Specific examined encompass topical drug delivery, contact lenses, intravitreal ocular adhesives, vitreous substitutes, cell‐based therapy. A methodical analysis, including an overview relevant structures comparative evaluation hydrogel‐based solutions against traditional treatments, is conducted. Additionally, potential constraints, translation challenges, knowledge gaps, research areas are identified. Our approach, guided by extensive literature from 2017 to 2023, illuminates unprecedented opportunities offered along pinpointing for further inquiry facilitate transition clinical practice.
Язык: Английский
Процитировано
10
International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(10), С. 5479 - 5479
Опубликована: Май 17, 2024
Corneal neovascularization can impair vision and result in a poor quality of life. The pathogenesis involves complex interplay angiogenic factors, notably vascular endothelial growth factor (VEGF). This review provides comprehensive overview potential therapies for corneal neovascularization, covering tissue inhibitors metalloproteinases (TIMPs), transforming beta (TGF-β) inhibitors, interleukin-1L receptor antagonist (IL-1 Ra), nitric oxide synthase (NOS) isoforms, galectin-3 retinal pigment epithelium-derived (PEDF), platelet-derived (PDGF) surgical treatments. Conventional treatments include anti-VEGF therapy laser interventions, while emerging such as immunosuppressive drugs (cyclosporine rapamycin) have been explored. Losartan decorin are antifibrotic agents that mitigate TGF-β-induced fibrosis. Ocular nanosystems innovative drug-delivery platforms facilitate the targeted release therapeutic agents. Gene therapies, small interfering RNA antisense oligonucleotides, promising approaches selectively inhibiting angiogenesis-related gene expression. Aganirsen is efficacious reducing area without significant adverse effects. These multifaceted underscore management complexity highlight ideas enhancing outcomes. Furthermore, importance combination need further research to develop specific considering their efficacy effects discussed.
Язык: Английский
Процитировано
8
Progress in Retinal and Eye Research, Год журнала: 2024, Номер 102, С. 101275 - 101275
Опубликована: Май 24, 2024
Язык: Английский
Процитировано
7
Stem Cell Research & Therapy, Год журнала: 2024, Номер 15(1)
Опубликована: Фев. 14, 2024
Corneal alkali burns can lead to ulceration, perforation, and even corneal blindness due epithelial defects extensive cell necrosis, resulting in poor healing outcomes. Previous studies have found that chitosan-based situ hydrogel loaded with limbal epithelium stem cells (LESCs) has a certain reparative effect on burns. However, the inconsistent pore sizes of carriers low loading rates resulted suboptimal repair In this study, 4D bioprinting technology was used prepare thermosensitive gel carrier (4D-CTH) uniform size adjustable shape improve transfer capacity LESCs. Prepare solutions chitosan acetate, carboxymethyl chitosan, β-glycerophosphate sodium at specific concentrations, mix them proportions create pore-size scaffold using technology. Extract culture rat LESCs (rLESCs) vitro, perform immunofluorescence experiments observe positivity rate deltaNp63 for identification. Conduct series validate compatibility 4D-CTH, including CCK-8 assay assess toxicity, scratch evaluate 4D-CTH rLESCs migration, Calcein-AM/PI staining experiment examine impact proliferation morphology. Establish severe burn model corneas, transplant onto injured cornea periodically opacity neovascularization slit lamp, by fluorescein staining. Assess therapeutic 4D-CTH-loaded through histological evaluation tissue paraffin sections stained hematoxylin eosin, as well frozen sections. Using were transferred wounds rats. Compared traditional treatment group (chitosan encapsulating rLESCs), 4D-CTH-rLESC had significantly higher efficiency injury, such lower score (1.2 ± 0.4472 vs 0.4 0.5477, p < 0.05) (5.5 1.118 2.6 0.9618, 0.01), wound (72.09 3.568% 86.60 5.004%, 0.01). summary, corneas similar normal complete structure. These findings suggested encapsulated accelerated after be considered rapid effective method treating defects.
Язык: Английский
Процитировано
6
Biomedicines, Год журнала: 2024, Номер 12(6), С. 1303 - 1303
Опубликована: Июнь 12, 2024
Diabetes mellitus (DM) is a chronic metabolic disorder marked by hyperglycemia due to defects in insulin secretion, action, or both, with global prevalence that has tripled recent decades. This condition poses significant public health challenges, affecting individuals, healthcare systems, and economies worldwide. Among its numerous complications, ocular surface disease (OSD) concern, yet understanding pathophysiology, diagnosis, management remains challenging. review aims explore the epidemiology, clinical manifestations, diagnostic approaches, strategies of diabetes-related OSD. The surface, including cornea, conjunctiva, associated structures, vital for maintaining eye health, lacrimal functional unit (LFU) playing crucial role tear film regulation. In DM, changes glycosaminoglycan metabolism, collagen synthesis, oxygen consumption, LFU dysfunction contribute complications. Persistent leads expression cytokines, chemokines, cell adhesion molecules, resulting neuropathy, abnormalities, epithelial lesions. Recent advances molecular research therapeutic modalities, such as gene stem therapies, show promise managing diabetic Future should focus on pathogenetically oriented therapies neuropathy keratopathy, transitioning from animal models trials improve patient outcomes.
Язык: Английский
Процитировано
6
International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(14), С. 7967 - 7967
Опубликована: Июль 19, 2022
A transparent cornea is paramount for vision. Corneal opacity one of the leading causes blindness. Although conventional corneal transplantation has been successful in recovering patients’ vision, outcomes are challenged by a global lack donor tissue availability. Bioengineered tissues gaining momentum as new source wound healing and scar management. Extracellular matrix (ECM)-scaffold-based engineering offers perspective on regenerative medicine. Ultrathin stromal laminar obtained from lenticule-based refractive correction procedures, such SMall Incision Lenticule Extraction (SMILE), an accessible novel collagen-rich ECM scaffolds with high mechanical strength, biocompatibility, transparency. After customization (including decellularization), these lenticules can serve acellular scaffold niche to repopulate cells, including keratocytes stem functional phenotypes. The intrastromal cell/tissue composites regenerate native-like restore This review highlights current status ECM-scaffold-based along development drug growth factor delivery systems, elucidates potential uses lenticule therapeutics.
Язык: Английский
Процитировано
25
Heliyon, Год журнала: 2024, Номер 10(10), С. e30970 - e30970
Опубликована: Май 1, 2024
Ultrathin electrospun poly (l-lactide-
Процитировано
4
Ophthalmic and Physiological Optics, Год журнала: 2024, Номер 45(1), С. 315 - 332
Опубликована: Ноя. 4, 2024
Abstract The cornea is one of the tissues responsible for covering and protecting inner structures eye. Central corneal thickness (CCT) defined as distance between anterior epithelial surface posterior endothelial layer. This parameter plays a very important role regarding intraocular pressure (IOP) measurement, evaluation uniformity, selection suitable technique refractive surgery planning surgical procedures to overcome disease. comprehensive review elucidates multifaceted factors influencing central thickness. Recognising impact these not only enhances our understanding dynamics but also contributes significantly refinement diagnostic therapeutic strategies in ophthalmology.
Язык: Английский
Процитировано
4
Investigative Ophthalmology & Visual Science, Год журнала: 2025, Номер 66(2), С. 35 - 35
Опубликована: Фев. 12, 2025
Neuropathic corneal pain (NCP) is a debilitating condition affecting millions of people worldwide. Despite their critical importance, currently available animal models for NCP research are limited by complex surgeries with high-risk strategy. To advance fundamental understanding NCP, we developed novel rodent model that explores both structural and functional mechanisms the disease, offering comprehensive approach. By uplifting (2-3 mm transversely) long ciliary nerve (LCN) gentle force (0.09 ± 0.02 newton [N]) pressure (0.18 0.05 MPa), our pulled mimics human conditions was investigated alongside normal control, sham full transection groups. Specifically, quantified status establishing relationship between perception chemical sensitivity, using Stevens' Power Law concept. Following surgery, temporal patterns heightened showed consistent trends across different stimulus methods, suggesting von Frey tests could effectively evaluate progression. The discernable differences in Alpha values (exponent) pain-perception curves nerve, groups (0.175 0.035, 0.235 0.015, 0.275 0.005, respectively) demonstrate model's sensitivity to changes status. Histological analysis revealed LCN elongation, thickening, alterations models, reduced satellite glial cells (SGCs) trigeminal ganglion compared control models. Krt16 gene expression significantly upregulated following surgery. Our not only delineates pathological landscape but also promises accelerate development targeted therapies.
Язык: Английский
Процитировано
0