Progress in molecular biology and translational science, Год журнала: 2023, Номер unknown
Опубликована: Янв. 1, 2023
Язык: Английский
Small, Год журнала: 2023, Номер 20(17)
Опубликована: Дек. 1, 2023
Abstract As one of the most common complications, infection causes majority mortality in cancer patients. However, therapeutic strategies that can simultaneously suppress tumors and protect patients from have been rarely reported. Here, use dual‐antigen‐displaying nanovaccines (DADNs) is described to elicit synergistic immunoactivation for treating preventing infectious complications. DADNs are prepared by wrapping immunoadjuvant‐loaded nanoparticles with a hybrid coating, which fused cell membranes separately genetically engineered express tumor pathogenic antigens. Due presence dual‐antigen combination, able promote maturation dendritic cells more importantly trigger cross‐presentation both combined During vivo investigations, we find reverse immunosuppression stimulating tumor‐associated antigen‐specific T‐cell responses, resulting significantly delayed growth mice. These also effective protective immunity against challenges induce robust production immunoglobulin G antibody prophylactic study. This work offers unique approach develop dual‐mode vaccines, promising synchronously infection.
Язык: Английский
Процитировано
5
Frontiers in Bioengineering and Biotechnology, Год журнала: 2023, Номер 11
Опубликована: Апрель 25, 2023
Regulation of research on microbes that cause disease in humans has historically been focused taxonomic lists ‘bad bugs’. However, given our increased knowledge these pathogens through inexpensive genome sequencing, 5 decades microbial pathogenesis, and the burgeoning capacity synthetic biologists, limitations this approach are apparent. With heightened scientific public attention biosafety biosecurity, an ongoing review by US authorities dual-use oversight, article proposes incorporation sequences concern (SoCs) into biorisk management regime governing genetic engineering pathogens. SoCs enable pathogenesis all infecting hosts ‘of concern’ to human civilization. Here we functions (FunSoCs) discuss how they might bring clarity potentially problematic outcomes involving infectious agents. We believe annotation with FunSoCs potential improve likelihood dual use is recognized both scientists regulators before it occurs.
Язык: Английский
Процитировано
4
Frontiers in Immunology, Год журнала: 2022, Номер 13
Опубликована: Июнь 2, 2022
Purpose The purpose of this article was to investigate the mechanism immune dysregulation COVID-19-related proteins in spinal tuberculosis (STB). Methods Clinical data were collected construct a nomogram model. C-index, calibration curve, ROC and DCA curve used assess predictive ability accuracy Additionally, 10 intervertebral disc samples for protein identification. Bioinformatics analyze differentially expressed (DEPs), including cells analysis, Gene Ontology (GO) KEGG pathway enrichment protein-protein interaction networks (PPI). Results predicted risk STB ranging from 0.01 0.994. C-index AUC training set 0.872 0.862, respectively. results external validation consistent with set. Immune scores indicated that B naive tissues significantly lower than non-TB tissues. Hub calculated by Degree, Closeness, MCC methods. main included Coronavirus disease-COVID-19. There 9 key intersection hub proteins. negative correlation between RPL19. associated genes. Conclusion Lymphocytes factors diagnosis STB. showed low expression Nine involved mechanisms. These nine may suppress regulating
Язык: Английский
Процитировано
6
Pharmacological Reports, Год журнала: 2022, Номер 74(6), С. 1315 - 1325
Опубликована: Авг. 5, 2022
Язык: Английский
Процитировано
5
Scientific Reports, Год журнала: 2023, Номер 13(1)
Опубликована: Дек. 4, 2023
Although an increased risk of myocarditis has been observed after vaccination with mRNA encoding severe acute respiratory syndrome coronavirus 2 spike protein, its underlying mechanism not elucidated. This study investigated the direct effects receptor-binding domain (S-RBD) on human cardiomyocytes differentiated from induced pluripotent stem cells (iPSC-CMs). Immunostaining experiments using ACE2 wild-type (WT) and knockout (KO) iPSC-CMs treated purified S-RBD demonstrated that was bound to internalized into subcellular space in iPSC-CMs, depending ACE2. combined live cell imaging a recombinant fused superfolder GFP (S-RBD-sfGFP) membrane, co-localized RAB5A, then delivered endosomes lysosomes iPSC-CMs. Quantitative PCR array analysis followed by single RNA sequence clarified S-RBD-sfGFP treatment significantly upregulated NF-kβ pathway-related gene (CXCL1) non-cardiomyocytes, while interferon (IFN)-responsive genes (IFI6, ISG15, IFITM3) matured cardiomyocytes. promoted protein ISGylation, ISG15-mediated post-translational modification ACE2-WT-iPSC-CMs, which suppressed ACE2-KO-iPSC-CMs. Our experimental demonstrates is through endolysosomal pathway, upregulates IFN-responsive promotes ISGylation
Язык: Английский
Процитировано
2
Infectious Diseases and Therapy, Год журнала: 2022, Номер 11(5), С. 1981 - 1998
Опубликована: Авг. 25, 2022
This study explored circulating pneumoproteins in the diagnosis, severity, and prognosis of COVID-19 by meta-analysis. We searched five databases other sources until December 16, 2021. Standardized mean difference (SMD) 95% confidence interval (CI) were overall outcomes. RevMan 5.3, Stata Meta-DiSc 1.4 utilized for pooled analysis. A total 2432 subjects from 26 studies included. Patients with had higher KL-6, SP-D, SP-A levels (SMD 1.34, CI [0.60, 2.08]; SMD 1.74, [0.64, 2.84]; 3.42, [1.31, 5.53], respectively) than healthy individuals. Circulating SP-D not significantly different survivors non-survivors − 0.19, [− 0.78, 0.40]). RAGE patients mild to moderate lower 0.93, 1.22, 0.65]; 1.32, 2.34, 0.29]; 1.17, 2.06, 0.28], severe COVID-19. Subgroup analysis suggested that country number may be related heterogeneity when analyzing vs. The meta-analysis diagnostic accuracy including KL-6 mortality, severity demonstrated they all limited value. Therefore, (KL-6, RAGEs) reflect COVID-19, follow-up are still needed.
Язык: Английский
Процитировано
3
Russian Journal of Infection and Immunity, Год журнала: 2024, Номер 14(2), С. 227 - 237
Опубликована: Авг. 5, 2024
Introduction. Viral capsid proteins can assemble into virus-like particles lacking infectivity and bearing parental virus antigens or artificially introduced from other pathogens. At least some of such are highly immunogenic could serve as a platform for promising vaccines. In this work, we assessed an effect decorated with SARS-CoV-2 spike protein fragment on human dendritic cell phenotype functional properties. Materials methods. The were assembled using chimeric molecules obtained by fusing genetic sequences encoding norovirus major VP1 coronavirus fragment, including the receptor-binding domain. Dendritic cells monocytes in vitro. Results. Incubation immature induced their phenotypic maturation. former was revealed significantly increased expression HLA-DR, CD80, CD86 CD83. after incubation at maximum concentration 10 μg/ml did not differ that mature positive control. Along maturation, caused manifold increase production pro-inflammatory tumor necrosis factor-α, anti-inflammatory interleukin-10, well interleukin-6, which stimulate both antibody synthesis cellular reactions. pronounced stimulation coated evidence about successful particle recognition. Finally, discuss plausible mechanisms recognition receptors. Conclusion. It has been shown is manifested markedly elevated functionally important membrane molecules, rise cytokines wide range. our opinion, data indicate promise based to display antigens.
Язык: Английский
Процитировано
0
Medicine in Drug Discovery, Год журнала: 2024, Номер unknown, С. 100198 - 100198
Опубликована: Сен. 1, 2024
Язык: Английский
Процитировано
0
Progress in molecular biology and translational science, Год журнала: 2023, Номер unknown
Опубликована: Янв. 1, 2023
Язык: Английский
Процитировано
0