Cited by T cell responses to SARS-COV-2

Dual‐Antigen‐Displaying Nanovaccines Elicit Synergistic Immunoactivation for Treating Cancer and Preventing Infectious Complications DOI

Fangjie Chen, Mengmeng Zhang, Fengmin Yang

et al.

Small, Journal Year: 2023, Volume and Issue: 20(17)

Published: Dec. 1, 2023

Abstract As one of the most common complications, infection causes majority mortality in cancer patients. However, therapeutic strategies that can simultaneously suppress tumors and protect patients from have been rarely reported. Here, use dual‐antigen‐displaying nanovaccines (DADNs) is described to elicit synergistic immunoactivation for treating preventing infectious complications. DADNs are prepared by wrapping immunoadjuvant‐loaded nanoparticles with a hybrid coating, which fused cell membranes separately genetically engineered express tumor pathogenic antigens. Due presence dual‐antigen combination, able promote maturation dendritic cells more importantly trigger cross‐presentation both combined During vivo investigations, we find reverse immunosuppression stimulating tumor‐associated antigen‐specific T‐cell responses, resulting significantly delayed growth mice. These also effective protective immunity against challenges induce robust production immunoglobulin G antibody prophylactic study. This work offers unique approach develop dual‐mode vaccines, promising synchronously infection.

Language: Английский

Citations

Improved understanding of biorisk for research involving microbial modification using annotated sequences of concern DOI

Gene D. Godbold, F. Curtis Hewitt, Anthony D. Kappell

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2023, Volume and Issue: 11

Published: April 25, 2023

Regulation of research on microbes that cause disease in humans has historically been focused taxonomic lists ‘bad bugs’. However, given our increased knowledge these pathogens through inexpensive genome sequencing, 5 decades microbial pathogenesis, and the burgeoning capacity synthetic biologists, limitations this approach are apparent. With heightened scientific public attention biosafety biosecurity, an ongoing review by US authorities dual-use oversight, article proposes incorporation sequences concern (SoCs) into biorisk management regime governing genetic engineering pathogens. SoCs enable pathogenesis all infecting hosts ‘of concern’ to human civilization. Here we functions (FunSoCs) discuss how they might bring clarity potentially problematic outcomes involving infectious agents. We believe annotation with FunSoCs potential improve likelihood dual use is recognized both scientists regulators before it occurs.

Language: Английский

Citations

Mechanism of COVID-19-Related Proteins in Spinal Tuberculosis: Immune Dysregulation DOI

Liyi Chen, Chong Liu, Tuo Liang

et al.

Frontiers in Immunology, Journal Year: 2022, Volume and Issue: 13

Published: June 2, 2022

Purpose The purpose of this article was to investigate the mechanism immune dysregulation COVID-19-related proteins in spinal tuberculosis (STB). Methods Clinical data were collected construct a nomogram model. C-index, calibration curve, ROC and DCA curve used assess predictive ability accuracy Additionally, 10 intervertebral disc samples for protein identification. Bioinformatics analyze differentially expressed (DEPs), including cells analysis, Gene Ontology (GO) KEGG pathway enrichment protein-protein interaction networks (PPI). Results predicted risk STB ranging from 0.01 0.994. C-index AUC training set 0.872 0.862, respectively. results external validation consistent with set. Immune scores indicated that B naive tissues significantly lower than non-TB tissues. Hub calculated by Degree, Closeness, MCC methods. main included Coronavirus disease-COVID-19. There 9 key intersection hub proteins. negative correlation between RPL19. associated genes. Conclusion Lymphocytes factors diagnosis STB. showed low expression Nine involved mechanisms. These nine may suppress regulating

Language: Английский

Citations

Jacareubin inhibits TLR4-induced lung inflammatory response caused by the RBD domain of SARS-CoV-2 Spike protein DOI

Deisy Segura-Villalobos, Daniela Roa-Velázquez,

Dan I Zavala-Vargas

et al.

Pharmacological Reports, Journal Year: 2022, Volume and Issue: 74(6), P. 1315 - 1325

Published: Aug. 5, 2022

Language: Английский

Citations

SARS-CoV-2 spike receptor-binding domain is internalized and promotes protein ISGylation in human induced pluripotent stem cell-derived cardiomyocytes DOI

Shota Okuno, Shuichiro Higo, Takumi Kondo

et al.

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: Dec. 4, 2023

Although an increased risk of myocarditis has been observed after vaccination with mRNA encoding severe acute respiratory syndrome coronavirus 2 spike protein, its underlying mechanism not elucidated. This study investigated the direct effects receptor-binding domain (S-RBD) on human cardiomyocytes differentiated from induced pluripotent stem cells (iPSC-CMs). Immunostaining experiments using ACE2 wild-type (WT) and knockout (KO) iPSC-CMs treated purified S-RBD demonstrated that was bound to internalized into subcellular space in iPSC-CMs, depending ACE2. combined live cell imaging a recombinant fused superfolder GFP (S-RBD-sfGFP) membrane, co-localized RAB5A, then delivered endosomes lysosomes iPSC-CMs. Quantitative PCR array analysis followed by single RNA sequence clarified S-RBD-sfGFP treatment significantly upregulated NF-kβ pathway-related gene (CXCL1) non-cardiomyocytes, while interferon (IFN)-responsive genes (IFI6, ISG15, IFITM3) matured cardiomyocytes. promoted protein ISGylation, ISG15-mediated post-translational modification ACE2-WT-iPSC-CMs, which suppressed ACE2-KO-iPSC-CMs. Our experimental demonstrates is through endolysosomal pathway, upregulates IFN-responsive promotes ISGylation

Language: Английский

Citations

Clinical Utility of Circulating Pneumoproteins as Diagnostic and Prognostic Biomarkers in COVID-19: A Systematic Review and Meta-analysis DOI

Yani Ke,

Yuqing Zhu,

Shuaihang Chen

et al.

Infectious Diseases and Therapy, Journal Year: 2022, Volume and Issue: 11(5), P. 1981 - 1998

Published: Aug. 25, 2022

This study explored circulating pneumoproteins in the diagnosis, severity, and prognosis of COVID-19 by meta-analysis. We searched five databases other sources until December 16, 2021. Standardized mean difference (SMD) 95% confidence interval (CI) were overall outcomes. RevMan 5.3, Stata Meta-DiSc 1.4 utilized for pooled analysis. A total 2432 subjects from 26 studies included. Patients with had higher KL-6, SP-D, SP-A levels (SMD 1.34, CI [0.60, 2.08]; SMD 1.74, [0.64, 2.84]; 3.42, [1.31, 5.53], respectively) than healthy individuals. Circulating SP-D not significantly different survivors non-survivors − 0.19, [− 0.78, 0.40]). RAGE patients mild to moderate lower 0.93, 1.22, 0.65]; 1.32, 2.34, 0.29]; 1.17, 2.06, 0.28], severe COVID-19. Subgroup analysis suggested that country number may be related heterogeneity when analyzing vs. The meta-analysis diagnostic accuracy including KL-6 mortality, severity demonstrated they all limited value. Therefore, (KL-6, RAGEs) reflect COVID-19, follow-up are still needed.

Language: Английский

Citations

Coronavirus spike protein fragment-containing chimeric virus-like particles stimulate human dendritic cell maturation DOI

V.Yu. Talayev, Д. В. Новиков, И Е Заиченко

et al.

Russian Journal of Infection and Immunity, Journal Year: 2024, Volume and Issue: 14(2), P. 227 - 237

Published: Aug. 5, 2024

Introduction. Viral capsid proteins can assemble into virus-like particles lacking infectivity and bearing parental virus antigens or artificially introduced from other pathogens. At least some of such are highly immunogenic could serve as a platform for promising vaccines. In this work, we assessed an effect decorated with SARS-CoV-2 spike protein fragment on human dendritic cell phenotype functional properties. Materials methods. The were assembled using chimeric molecules obtained by fusing genetic sequences encoding norovirus major VP1 coronavirus fragment, including the receptor-binding domain. Dendritic cells monocytes in vitro. Results. Incubation immature induced their phenotypic maturation. former was revealed significantly increased expression HLA-DR, CD80, CD86 CD83. after incubation at maximum concentration 10 μg/ml did not differ that mature positive control. Along maturation, caused manifold increase production pro-inflammatory tumor necrosis factor-α, anti-inflammatory interleukin-10, well interleukin-6, which stimulate both antibody synthesis cellular reactions. pronounced stimulation coated evidence about successful particle recognition. Finally, discuss plausible mechanisms recognition receptors. Conclusion. It has been shown is manifested markedly elevated functionally important membrane molecules, rise cytokines wide range. our opinion, data indicate promise based to display antigens.

Language: Английский

Citations

Coronavirus spike protein-based vaccines. Vaccine delivery systems DOI

Akmal M. Asrorov, Mirzakamol S. Ayubov, Bin Tu

et al.

Medicine in Drug Discovery, Journal Year: 2024, Volume and Issue: unknown, P. 100198 - 100198

Published: Sept. 1, 2024

Language: Английский

Citations

T cell responses to SARS-COV-2 DOI

Laiqha Khadri,

Mohammad Hossein Ziraksaz,

Ahmad Bashir Barekzai

et al.

Progress in molecular biology and translational science, Journal Year: 2023, Volume and Issue: unknown

Published: Jan. 1, 2023

Language: Английский

Citations