Thymol promotes cell proliferation and protects against LPS-induced intestinal inflammation via activation of the EGFR/PI3K pathway

Food Science and Biotechnology, Год журнала: 2025, Номер 34(10), С. 2281 - 2294

Опубликована: Фев. 24, 2025

Язык: Английский

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Comprehensive analysis of cellular metrics: From proliferation to mitochondrial membrane potential and cell death in a single sample

Cell Death Discovery, Год журнала: 2025, Номер 11(1)

Опубликована: Март 24, 2025

Abstract Changes in cell number during vitro experiments and pharmacological screenings primarily depend on two factors: death proliferation. The dynamics of these processes determine whether populations expand accumulate or, conversely, decrease over time. Understanding the biological mechanisms governing changes is crucial for deciphering mode action any or genetic treatment fundamental research pre-clinical trials. In this context, we introduce a robust efficient flow cytometry-based methodology that enables comprehensive analysis key cellular parameters indicate numbers. This approach encompasses assessment count along with critical maintenance including proliferation, cycle dynamics, apoptosis, permeability, mitochondrial depolarization. These are intricately linked, offering detailed view state. described versatile adaptable analyzing various types, at steady state response to treatments. To develop workflow, integrated optimised multiple stainings such as annexin V, propidium iodide, bromodeoxyuridine, CellTrace Violet, JC-1 into unified protocol. article offers detailed, step-by-step guide entire method, covering aspects timing, sample preparation techniques, reagents used. Additionally, it includes examples data can be obtained technique illustrates its multiparametric visualization. Collectively, facilitates rapid acquisition up eight different from single one experiment.

Язык: Английский

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Potential therapeutic target for polysaccharide inhibition of colon cancer progression

Frontiers in Medicine, Год журнала: 2024, Номер 10

Опубликована: Янв. 8, 2024

In recent years, colon cancer has become one of the most common malignant tumors worldwide, posing a great threat to human health. Studies have shown that natural polysaccharides rich biological activities and medicinal value, such as anti-inflammatory, anti-cancer, anti-oxidation, immune-enhancing effects, especially with potential anti-colon mechanisms. Natural can not only protect enhance homeostasis intestinal environment but also exert direct inhibition effect on cells, making it promising strategy for treating cancer. Preliminary clinical experiments demonstrated oral administration low high doses citrus pectin reduce tumor volume in mice by 38% (

Язык: Английский

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Immunomodulation of Cancer Cells Using Autologous Blood Concentrates as a Patient-Specific Cell Culture System: A Comparative Study on Osteosarcoma and Fibrosarcoma Cell Lines

Bioengineering, Год журнала: 2024, Номер 11(4), С. 303 - 303

Опубликована: Март 23, 2024

The understanding that tumor cells might evade immunity through various mutations and the potential of an augmented immune system to eliminate abnormal led idea utilizing platelet-rich fibrin (PRF), a blood concentrate containing body’s elements as adjunctive therapy for localized tumors. This study is first evaluated effect PRF generated with different relative centrifugal forces (RCFs) on osteoblastic fibroblastic cell lines MG63 HT1080 regard viability, cytokine growth factor release, gene expression factors related cycle apoptosis. Our findings could demonstrate decreased proliferation when treated indirectly compared cultures without PRF. was more distinct were low-RCF PRF, where higher concentrations cytokines reduced RCFs can be found. Similar patterns observed assessing regulation apoptosis in both Despite variations, there consistent trend up-regulation tumor-suppressive genes down-regulation anti-apoptotic types following treatment high- and, particularly, formulations.

Язык: Английский

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Anti-obesity effects of a standardized ethanol extract of Eisenia bicyclis by regulating the AMPK signaling pathway in 3T3-L1 cells and HFD-induced mice

Food & Function, Год журнала: 2024, Номер 15(12), С. 6424 - 6437

Опубликована: Янв. 1, 2024

Obesity requires treatment to mitigate the potential development of further metabolic disorders, including diabetes, hyperlipidemia, tumor growth, and non-alcoholic fatty liver disease. We investigated anti-obesity effect a 30% ethanol extract Eisenia bicyclis (Kjellman) Setchell (EEB) on 3T3-L1 preadipocytes high-fat diet (HFD)-induced obese C57BL/6 mice. Adipogenesis transcription factors peroxisome proliferator-activated receptor (PPAR)γ, CCAAT/enhancer-binding protein-alpha (C/EBPα), sterol regulatory element-binding protein-1 (SREBP-1) were ameliorated through AMP-activated protein kinase (AMPK) pathway by EEB in differentiated cells. attenuated mitotic clonal expansion upregulating cyclin-dependent inhibitors (CDKIs) while downregulating cyclins CDKs. In HFD-fed mice, significantly decreased total body weight, fat tissue tissue. The expression PPARγ, C/EBPα, SREBP-1 was increased subcutaneous tissues, levels these factors. also inhibited lipogenesis acetyl-CoA carboxylase (ACC) acid synthase (FAS) tissues. Moreover, phosphorylation AMPK ACC downregulated HFD-induced mouse group, whereas administration improved phosphorylation; thus, may be related pathway. Histological analysis showed that reduced adipocyte size accumulation respectively. promotes thermogenesis brown adipose improves insulin leptin blood lipid profiles. Our results suggest could used as agent prevent obesity.

Язык: Английский

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Recent advancements in selenium nanoconstructs as a potential carrier in cancer therapy

Nano-Structures & Nano-Objects, Год журнала: 2024, Номер 40, С. 101399 - 101399

Опубликована: Ноя. 4, 2024

Язык: Английский

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LncRNA-ANRIL regulates CDKN2A to promote malignant proliferation of Kasumi-1 cells

Cell Division, Год журнала: 2025, Номер 20(1)

Опубликована: Янв. 28, 2025

This study aimed to investigate the regulatory effects of long non-coding RNA-ANRIL on CDKN2A in cell cycle Kasumi-1 cells and elucidate underlying molecular mechanisms. ANRIL expression levels were quantified using RT-qPCR peripheral blood samples from acute myeloid leukemia (AML) patients. knockdown efficiency was validated via RT-qPCR, distribution analyzed flow cytometry. Cell proliferation assays conducted with CCK-8 following palbociclib treatment downregulation. RNA immunoprecipitation (RIP) identified potential ANRIL-associated targets, while western blotting assessed GSK3β/β-catenin/cyclin D1 signaling components related proteins. markedly overexpressed AML patient samples. Knockdown led G1 phase arrest accompanied by reduced CDK2/4/6 cyclin protein levels, upregulation induced opposite effect. Palbociclib for 24 h 48 elevated population suppressed expression, demonstrating its ability counteract ANRIL-driven progression. Downregulation also GSK3β/β-catenin pathway, reducing whereas reactivated this axis. Co-transfection experiments showed that simultaneous suppression overexpression attenuated ANRIL's stimulatory RIP analysis confirmed a physical interaction between CDKN2A. Furthermore, downregulation inhibited reversed pathway activation mediated upregulation. facilitates survival modulating activate pathway.

Язык: Английский

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Superparamagnetic Iron Oxide Nanoparticles Induce Apoptosis in HT-29 Cells by Stimulating Oxidative Stress and Damaging DNA

Biological Trace Element Research, Год журнала: 2022, Номер 201(3), С. 1163 - 1173

Опубликована: Апрель 22, 2022

Язык: Английский

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Photodynamic therapy induced cell cycle arrest and cancer cell synchronization: review

Frontiers in Oncology, Год журнала: 2023, Номер 13

Опубликована: Июль 12, 2023

Cell cycle arrest (CCA) is seen as a prime candidate for effective cancer therapy. This mechanism can help researchers to create new treatments target cells at particular stages of the cell (CC). The CCA characteristic various therapeutic modalities, including radiation (RT) and chemotherapy (CT), which synchronizes facilitates standardization radio-chemotherapy protocols. Although it was discovered that photodynamic treatment (PDT) had biological effect on in cells, remains unclear. Furthermore, besides conventional forms death such apoptosis, autophagy, necrosis, unconventional types pyroptosis, mitotic catastrophe, paraptosis, ferroptosis, necroptosis, parthanatos after PDT have been reported. Thus, variety elements, oxygen, tumor’s microenvironment, characteristics light, photosensitizer (PS), influence effectiveness treatment, not yet studied clearly. review focuses induced by PSs agents lines. be viewed remarkable instructive management protocol. Regarding relationship between quantity reactive oxygen species (ROS) its consequences, we proposed two mathematical models PDT. Finally, gathered recent vitro vivo studies about post-PDT made suggestions how standardize, potentiate, customize methodology.

Язык: Английский

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Cyclin-dependent kinases in cancer: Role, regulation, and therapeutic targeting

Advances in protein chemistry and structural biology, Год журнала: 2023, Номер unknown, С. 21 - 55

Опубликована: Янв. 1, 2023

Язык: Английский

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