Journal of Biophotonics,
Год журнала:
2023,
Номер
17(2)
Опубликована: Ноя. 17, 2023
We
investigated
the
effects
of
low-level
Er:YAG
laser
irradiation
on
proliferation
and
alternations
in
early
gene
expression
gingival
fibroblasts.
Mice
primary
fibroblasts
were
irradiated
with
an
(1.8,
3.9,
5.8
J/cm2).
Irradiation
at
3.9
J/cm2
promoted
cell
without
significant
changes
lactate
dehydrogenase
or
Hspa1a
expression.
Three
hours
after
J/cm2,
Fn1
level
was
significantly
increased.
RNA-seq
identified
15
differentially
expressed
genes
between
non-irradiated
cells,
some
which
belonged
to
immediate
(IEGs).
Kyoto
Encyclopedia
Genes
Genomes
pathway
analysis
indicated
MAPK
enhancement,
set
enrichment
showed
TGF-β
signaling
set.
Enhanced
via
disappeared
upon
inhibition
Dusp4,
Dusp5,
Tgfr1
Low-level
irradiation,
especially
a
major
temperature
elevation,
enhanced
fibroblast
proliferation,
following
IEG
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(5), С. 2763 - 2763
Опубликована: Фев. 27, 2024
The
extracellular
matrix
(ECM)
is
a
complex
non-cellular
three-dimensional
macromolecular
network
present
within
all
tissues
and
organs,
forming
the
foundation
on
which
cells
sit,
composed
of
proteins
(such
as
collagen),
glycosaminoglycans,
proteoglycans,
minerals,
water.
ECM
provides
fundamental
framework
for
cellular
constituents
tissue
biochemical
support
to
surrounding
cells.
highly
dynamic
structure
that
constantly
being
remodeled.
Matrix
metalloproteinases
(MMPs)
are
among
most
important
proteolytic
enzymes
capable
degrading
molecules.
MMPs
play
relevant
role
in
physiological
well
pathological
processes;
participate
embryogenesis,
morphogenesis,
wound
healing,
remodeling,
therefore,
their
impaired
activity
may
result
several
problems.
MMP
also
associated
with
chronic
inflammation,
breakdown,
fibrosis,
cancer
invasion
metastasis.
periodontium
unique
anatomical
site,
variety
connective
tissues,
created
by
ECM.
During
periodontitis,
inflammation
affecting
periodontium,
increased
presence
observed,
resulting
irreversible
losses
periodontal
tissues.
expression
be
controlled
various
ways,
one
inhibition
an
endogenous
group
inhibitors
(TIMPs),
reversion-inducing
cysteine-rich
protein
Kazal
motifs
(RECK).
National Science Review,
Год журнала:
2024,
Номер
11(8)
Опубликована: Июнь 26, 2024
ABSTRACT
Periodontitis
involves
hyperactivated
stromal
cells
that
recruit
immune
cells,
exacerbating
inflammation.
This
study
presents
an
ATP-responsive
metal–organic
framework
(Mg/Zn-MOF)
designed
for
periodontitis
treatment,
utilizing
ion
interference
to
modulate
responses
and
prevent
tissue
destruction.
Addressing
the
challenges
of
synergistic
effects
targeted
delivery
faced
by
traditional
immunomodulatory
nanomaterials,
Mg/Zn-MOF
system
is
activated
extracellular
ATP—a
pivotal
molecule
in
pathology—ensuring
release.
Magnesium
zinc
ions
released
from
synergistically
inhibit
membrane
pore
formation
attenuating
Gasdermin
D
(GSDMD)
expression
activation.
action
curtails
pyroptosis,
lactate
dehydrogenase
IL-1β
release,
thwarting
onset
inflammatory
cascades.
Mechanistically,
intervenes
both
NLRP3/Caspase-1/GSDMD
Caspase-11/GSDMD
pathways
mitigate
pyroptosis.
In
vivo
assessments
confirm
its
effectiveness
diminishing
cell
infiltration
preserving
collagen
integrity,
thereby
safeguarding
against
periodontal
damage
bone
loss.
investigation
highlights
promise
ion-interference
strategies
immunotherapy,
representing
a
significant
stride
developing
therapeutic
approaches.
Journal of Functional Biomaterials,
Год журнала:
2025,
Номер
16(1), С. 25 - 25
Опубликована: Янв. 14, 2025
Oral
diseases
such
as
dental
caries,
periodontitis,
and
oral
cancer
are
prevalent
present
significant
challenges
to
global
public
health.
Although
these
typically
treated
through
procedures
like
preparation
resin
filling,
scaling
root
planning,
or
surgical
excision,
interventions
often
not
entirely
effective,
postoperative
drug
therapy
is
usually
required.
Traditional
treatments,
however,
limited
by
factors
poor
penetration,
side
effects,
the
development
of
resistance.
As
a
result,
there
growing
need
for
novel
delivery
systems
that
can
enhance
therapeutic
efficacy,
reduce
improve
treatment
outcomes.
In
recent
years,
drug-loaded
vesicles,
liposomes,
polymersomes,
extracellular
vesicles
(EVs),
have
emerged
promising
platforms
due
their
high
encapsulation
efficiency,
controlled
release
properties,
excellent
biocompatibility.
This
review
provides
an
in-depth
examination
characteristics,
advantages,
limitations
in
context
disease
treatment.
It
further
explores
reasons
advantages
discusses
specific
applications,
prospects,
strategies
optimizing
vesicle-based
improved
clinical
Oral Diseases,
Год журнала:
2024,
Номер
30(7), С. 4767 - 4781
Опубликована: Фев. 26, 2024
This
study
aimed
to
clarify
the
difference
in
Fusobacterium
nucleatum
(F.
nucleatum)
induced
inflammatory
cytokines
and
nod-like
receptor
protein
3
(NLRP3)
inflammasomes
dysregulation
among
three
periodontal
cells.
Clinical Science,
Год журнала:
2024,
Номер
138(12), С. 725 - 739
Опубликована: Июнь 1, 2024
Abstract
Objectives:
Clinical
studies
have
confirmed
that
galectin-3
(Gal-3)
levels
are
significantly
elevated
in
periodontitis
patients.
The
present
study
aimed
to
explore
the
effects
of
Gal-3
inhibition
on
periodontal
inflammation
vitro
and
vivo.
Methods:
Human
gingival
fibroblasts
(HGFs)
with
or
without
knockdown
were
stimulated
by
lipopolysaccharide
(LPS),
a
ligation-induced
mouse
model
treated
inhibitor
was
established.
Hematoxylin–eosin
(H&E)
immunohistochemistry
(IHC)
staining
used
evaluate
tissues.
Quantitative
real-time
polymerase
chain
reaction
(qRT-PCR)
enzyme-linked
immunosorbent
assay
(ELISA)
detect
Gal-3,
interleukin
(IL)-6,
IL-8,
C-C
motif
ligand
2
(CCL2)
expression.
Immunofluorescence
western
blotting
NF-κB
ERK
signaling
pathway
activation.
Micro-computed
tomography
analyse
degree
bone
loss.
Results:
up-regulated
inflamed
tissues
LPS-induced
HGFs.
markedly
decreased
IL-6,
CCL2
expression
blocked
activation
In
model,
alleviated
IL-1β
IL-6
infiltration
tissue
mitigated
Conclusions:
notably
partly
through
blocking
Periodontitis
is
an
inflammatory
disease
triggered
by
microbial
biofilms
that
promote
immune
dysfunction
and
tissue
destruction
of
tooth-supporting
tissues.
The
search
for
soluble
mediators
amplify
responses
matrix
degradation
in
periodontal
tissues
has
implicated
extracellular
vimentin
(ECV)
as
a
signaling
ligand
damage-associated
molecular
pattern
the
pathogenesis
periodontitis.
Intracellular
filaments
are
essential
structural
integrity
cells
preservation
homeostasis.
These
important
determinants
health
periodontium
many
other
organs.
But
inflamed
tissues,
intracellular
disassembled.
Vimentin
subsequently
released
from
into
space
form
where
it
drives
destruction.
We
discuss
role
ECV
molecule
several
apply
these
data
to
understand
how
diseases
like
periodontitis,
amplifies
contribute
progression.
Arising
data,
we
consider
novel
therapeutic
opportunities
limiting
targeting
treatment
disorders
Current Issues in Molecular Biology,
Год журнала:
2025,
Номер
47(2), С. 72 - 72
Опубликована: Янв. 23, 2025
Periodontal
diseases,
including
gingivitis
and
periodontitis,
are
chronic
inflammatory
conditions
of
the
teeth’
supporting
structures
that
can
lead
to
progressive
tissue
destruction
loss
if
left
untreated.
Basic
treatments
like
scaling
root
planing,
alone
or
combined
with
antimicrobial
agents,
standard
care.
However,
increasing
prevalence
antibiotic
resistance
need
for
new
ideas
in
therapy,
adjunctive
ozone
therapy
have
gained
attention.
Ozone
(O3),
a
triatomic
oxygen
molecule,
is
used
because
its
strong
antimicrobial,
anti-inflammatory,
regenerative
activity
and,
hence,
as
potential
tool
periodontal
therapy.
This
review
use
disease
breaks
down
multifaceted
mechanism
which
includes
selective
action
against
biofilm-associated
pathogens,
immunomodulatory
effects
on
host
cells,
stimulation
repair.
O3
disrupts
microbial
biofilms,
enhances
immune
cell
function,
promotes
healing
by
activating
Nuclear
Factor
Erythroid
2-Related
2
(Nrf2)
Mitogen-Activated
Protein
Kinase
(MAPK)
signaling
pathways
regulate
oxidative
stress,
inflammation,
apoptosis.
Additional
findings
include
ability
upregulate
growth
factors
extracellular
matrix
proteins,
significant
regeneration.
also
discusses
application
lines,
emphasizing
impact
viability,
proliferation,
differentiation.
Advances
techniques,
properties
O3,
demonstrated
clinical
benefits.
Challenges,
standardized
dosages,
effective
delivery
systems,
long-term
studies,
addressed
ensure
safe
integration.
dual
capabilities,
offers
an
innovative
approach
treatment.
Future
research
focusing
optimized
protocols
evidence-based
guidelines
essential
fully
realize
enhancing
health
improving
patient
outcomes.
