STAR Protocols, Год журнала: 2024, Номер 5(4), С. 103444 - 103444
Опубликована: Ноя. 7, 2024
Язык: Английский
International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(2), С. 1208 - 1208
Опубликована: Янв. 19, 2024
The lacrimal gland is responsible for maintaining the health of ocular surface through production tears. However, our understanding immune system within currently limited. Therefore, in this study, we utilized single-cell RNA sequencing and bioinformatic analysis to identify analyze cells molecules present glands normal mice. A total 34,891 were obtained from mice classified into 18 distinct cell clusters using Seurat clustering. Within these populations, 26 different subpopulations identified, including T cells, innate lymphocytes, macrophages, mast dendritic B cells. Network revealed complex cell-cell interactions between with particularly significant observed among plasma Interestingly, found be main source ligands Thy1 signaling pathway, while M2 macrophages identified as primary target pathway. Moreover, some validated immunohistological techniques. Collectively, findings highlight abundance provide valuable insights complexity its relevance associated diseases.
Язык: Английский
Процитировано
6
Investigative Ophthalmology & Visual Science, Год журнала: 2024, Номер 65(4), С. 46 - 46
Опубликована: Апрель 30, 2024
The lacrimal gland (LG) is the main organ responsible for tear secretion and an important pathogenic site dry eye disease (DED). This study aimed to comprehensively characterize LG cellular heterogeneity under normal DED conditions using single-nucleus RNA sequencing (snRNA-seq).
Язык: Английский
Процитировано
3
The Ocular Surface, Год журнала: 2024, Номер 34, С. 60 - 95
Опубликована: Июнь 28, 2024
Язык: Английский
Процитировано
3
Proceedings of the National Academy of Sciences, Год журнала: 2023, Номер 120(42)
Опубликована: Окт. 9, 2023
The lacrimal gland is of central interest in ophthalmology both as the source aqueous component tear fluid and site autoimmune pathology context Sjogren's syndrome (SjS). To provide a foundational description mouse cell types their patterns gene expression, we have analyzed single-cell transcriptomes from wild-type (Balb/c) mice two genetically based SjS models,
Язык: Английский
Процитировано
7
The Ocular Surface, Год журнала: 2024, Номер 33, С. 50 - 63
Опубликована: Май 3, 2024
To investigate the global transcriptional landscape of lacrimal gland cell populations in GVHD mouse model. Single-cell RNA sequencing and further bioinformatic analysis dissociated (LG) cells from model were performed. Parts results confirmed by immunofluorescence staining. We identified 23 belonging to 11 types. In LG, proportion acinar cells, myoepithelial endothelial was remarkably decreased, while T macrophages significantly expanded. Gene expression indicated decreased secretion function, extracellular matrix (ECM) synthesis, increased chemokines cells. A newly described epithelial population named Lrg1high expressing distinct gene signatures, exclusively LG. The fibroblasts exhibited an inflammation pattern. pattern suggested ability recruit immune damaged cell-cell junctions. mainly comprised Th2 effective memory CD8+ a cell-linked This single-cell atlas uncovered alterations patterns constructed communication networks These data may provide some new insight into understanding development ocular GVHD.
Язык: Английский
Процитировано
2
The Ocular Surface, Год журнала: 2024, Номер 34, С. 283 - 295
Опубликована: Авг. 28, 2024
Язык: Английский
Процитировано
2
Materials, Год журнала: 2024, Номер 17(22), С. 5425 - 5425
Опубликована: Ноя. 6, 2024
The lacrimal gland (LG) is vital for ocular health, producing tears that lubricate and protect the eye. Dysfunction of LG leads to aqueous-deficient dry eye disease (DED), significantly impacting quality life. Current treatments mainly address symptoms rather than underlying dysfunction, highlighting need regenerative therapies. Tissue engineering offers a promising solution, with biomaterials playing crucial roles in scaffolding supporting cell growth regeneration. This review focuses on recent advances used tissue gland. We discuss both natural synthetic mimic extracellular matrix provide structural support proliferation differentiation. Natural biomaterials, such as Matrigel, decellularized matrices, chitosan, silk fibroin hydrogels, human amniotic membrane are evaluated their biocompatibility ability cells. Synthetic like polyethersulfone, polyesters, biodegradable polymers (PLLA PLGA), assessed mechanical properties potential create scaffolds replicate complex architecture LG. also explore integration factors stem cells these enhance Challenges achieving proper vascularization, innervation, long-term functionality engineered tissues discussed. Advances 3D bioprinting scaffold fabrication techniques highlighted avenues overcome current limitations.
Язык: Английский
Процитировано
2
The Ocular Surface, Год журнала: 2023, Номер 31, С. 63 - 80
Опубликована: Дек. 21, 2023
Язык: Английский
Процитировано
6
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Июль 1, 2024
Abstract Src family kinases (SFKs), including Src, Fyn and Yes, play important roles in development cancer. Despite being first discovered as the Y es- a ssociated p rotein, regulation of Yap by SFKs remains poorly understood. Here, through single-cell analysis genetic lineage tracing, we show that pan-epithelial ablation C-terminal kinase (Csk) lacrimal gland unleashes broad signaling but specifically causes extrusion apoptosis acinar progenitors at time when they are shielded myoepithelial cells from basement membrane. Csk mutants can be phenocopied constitutively active rescued deleting or Taz , indicating significant functional overlap between signaling. Although Src-induced tyrosine phosphorylation has long been believed to regulate activity, find mutating these residues both fails perturb mouse alleviate phenotype. In contrast, loses Hippo signaling-dependent serine translocates into nucleus mutants. Further chemical genetics studies demonstrate acute inhibition enhances Crk/CrkL Rac1 whereas removing Crk / CrkL Rap1 ameliorates mutant These results controls Hippo-Yap Crk/CrkL-Rac/Rap axis promote cell extrusion.
Язык: Английский
Процитировано
0
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Июль 22, 2024
Abstract Organ development relies on complex molecular mechanisms that guide initially homogeneous populations of stem cells to differentiate into specialized cell types within defined spatial patterns. While stable during homeostasis, the proper organization must be re-established in case tissue injury for successful regeneration organ shape and function. How commit a differentiation path is central question research; however, coordination between geometry fate specification remains enigmatic. To elucidate instructing self-organization symmetry breaking epithelial cells, we developed multi-faceted approach combining vitro organoids, ex vivo embryonic explants, single-cell quantitative imaging investigate dynamic acquisition four bi-layered epithelia, but also regeneration. Our findings indicate architecture primary determinant decisions these tissues. Upon initial internalization event, population break symmetry. Through genetic pharmacological perturbations, have demonstrated tightly coordinated interplay Hippo/YAP Notch signaling essential conveying information from functional potency restriction. Globally, this study uncovers inherent capacity self-organize multicellular structures, where precise position each differentiated critical instruct their choices
Язык: Английский
Процитировано
0