Isoquinoline‐Fused Benzimidazoles Based Highly Selective Fluorogenic Receptors for Detection of Cu²⁺, Fe³⁺, and Cl⁻ Ions: Cytotoxicity and HepG2 Cancer Cell Imaging

Luminescence, Год журнала: 2025, Номер 40(3)

Опубликована: Март 1, 2025

An efficient isoquinoline-fused benzimidazole-based "turn-on" fluorescence receptor 9,10-bis(2-phenylhydrazineyl)-7H-benzo[de]imidazo[2,1-a]isoquinolin-7-one OXPH(ANQ) and "turn-off" 9,10-bis((3-(1H-imidazol-1-yl)propyl)amino)-7H-benzo[de]imidazo[2,1-a]isoquinolin-7-one OXPID(ANQ) were prepared characterized by various spectral techniques. The sensing behavior of receptors was demonstrated UV-vis experiments, naked-eye detection exhibited prominent visual emission color change toward Cu2+/Cl- Fe3+ over other testing cations/anions in DMSO:water (9:1, ν/v) solution. 1:1 binding stoichiometry confirmed Job's plot, FT-IR, mass titration, also DFT studies with target ions as evidence for the nature OXPH(ANQ)/OXPID(ANQ) ions, respectively. Limits 4.50 4.91 μM, 5.06 μM. Interestingly, chemosensors applied to intracellular image living cells HepG2 (Liver cancer cells) via confocal microscopy technique at physiological environmental utilized antimicrobial activity.

Язык: Английский

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Effect of Pesticide Exposure over DNA Damage in Farmers from Los Reyes, Michoacan in Mexico

Toxics, Год журнала: 2023, Номер 11(2), С. 122 - 122

Опубликована: Янв. 26, 2023

In the municipality of Los Reyes, Michoacán, in Mexico, several economic activities coexist; however, most relevant is agriculture. It stands out as an agro-industrial center and commercial enclave region, suitable for cultivation sugar cane; currently fruit growing takes first place with blackberry, raspberry blueberry, followed by avocado, peach, strawberry other crops. A large quantity variety pesticides are applied to crops, consequently population at constant risk. This study aimed evaluate whether a factor genetic damage agricultural workers from using alkaline comet assay. Fifty-nine residents participated (41 18 controls). Results included confounding factors (alcohol consumption, smoking habit, gender, age, BMI, etc.) indicated non-significant statistical difference between two groups, higher DNA values that was than expected normal healthy unexposed population. seems control measures, safe handling quality standards, required producers so their products can be exported, have resulted less damage, despite workers’ activity, but reference still requires regular surveillance those exposed. The use protective equipment or measures reduce risk it also necessary promote service comply labor regulations workers.

Язык: Английский

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Trypsin-Based Chemoenzymatic Assay for Detection of Pollutants and Safety Assessment of Food Additives

Chemosensors, Год журнала: 2023, Номер 11(4), С. 237 - 237

Опубликована: Апрель 10, 2023

Chemoenzymatic assay systems are widely used to detect toxicants in various samples, including food and environment specimens. These methods based on the ability of types toxicant specifically inhibit/activate functions individual enzymes or enzyme systems. The present study examines possibility using proteolytic trypsin as a specific marker protease inhibitors different samples. shows that activity is not affected by heavy metals, pesticides, quinones at levels considerably greater than their maximum allowable concentrations (MACs) water bodies. At same time, IC50 value for preservative potassium sorbate (E202) 15 mg/L, which substantially lower its acceptable daily intake (ADI). quenching fluorescence presence suggests inhibition could occur due binding region adjacent active center. was immobilized starch gel ensure stability assay. Single-use reagents were prepared dry disks be stored over long periods. Their sensitivity copper (II) chloride, sorbate, chromium (III) chloride similar free trypsin.

Язык: Английский

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Enzyme Inhibition-Based Assay to Estimate the Contribution of Formulants to the Effect of Commercial Pesticide Formulations

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(3), С. 2268 - 2268

Опубликована: Янв. 23, 2023

Pesticides can affect the health of individual organisms and function entire ecosystem. Therefore, thorough assessment risks associated with use pesticides is a high-priority task. An enzyme inhibition-based assay used in this study as convenient quick tool to effects at molecular level. The contribution formulants toxicological properties pesticide formulations has been studied by analyzing 7 active ingredients (AIas) 10 commercial based on them (AIfs) wide range systems differing complexity (single-, coupled, three-enzyme systems). Results have compared AIas AIfs bioluminescence luminous bacterium Photobacterium phosphoreum. Mostly, produce considerably stronger inhibitory effect activity than AIas, which confirms formulation. current demonstrate that "inert" are not ecotoxicologically safe augment formulations; therefore, their should be controlled more strictly. Circular dichroism fluorescence spectra enzymes for assays do show any changes protein structure presence during procedure. This finding suggests through other mechanisms.

Язык: Английский

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Formulation and Characterization of Matrine Oil Dispersion to Improve Droplet Wetting and Deposition

Molecules, Год журнала: 2023, Номер 28(19), С. 6896 - 6896

Опубликована: Сен. 30, 2023

The unreasonable use of chemical pesticides has caused serious damage to crops and the ecological environment. botanical pesticide matrine attracted attention as an environmentally friendly pesticide. Compared with traditional spraying methods, unmanned aerial vehicle (UAV) advantages safety, rapidity, uniform droplets, low dosages, no terrain or crop restrictions. In this study, OD was prepared according application requirements flight prevention preparations using three different emulsifiers. stability, wettability, particle size distribution, performance were studied. results indicated that when amount emulsifier 8%, types had good stability. spray suspension VO/03 better than other two Therefore, 8% could be used for ultra-low-volume sprays applications. we provide a theoretical basis technical guidance develop formulations

Язык: Английский

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Assessing the Dual Toxicity of Hfo2 Nanoparticles and Quinalphoson Pila Virens

Опубликована: Янв. 1, 2024

Download This Paper Open PDF in Browser Add to My Library Share: Permalink Using these links will ensure access this page indefinitely Copy URL DOI

Язык: Английский

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Cytotoxicity induced by three commercial neonicotinoid insecticide formulations in differentiated human neuroblastoma SH-SY5Y cells

Toxicology Research, Год журнала: 2024, Номер 13(5)

Опубликована: Сен. 2, 2024

Neonicotinoid insecticides are used worldwide for crop protection. They act as agonists at postsynaptic nicotinic acetylcholine receptors (nAChRs), disrupting normal neurotransmission in target insects. Human exposure is high due to the widespread use of neonicotinoids and their residues food. This study aimed evaluate vitro neurotoxicity three neonicotinoid commercial formulations Much 600 FS® (imidacloprid g L

Язык: Английский

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Assessing the dual toxicity of HfO2 nanoparticles and quinalphos on Pila virens

The Science of The Total Environment, Год журнала: 2024, Номер 957, С. 177582 - 177582

Опубликована: Ноя. 27, 2024

Язык: Английский

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