Management of biofilm by an innovative layer-structured membrane for membrane biofilm reactor (MBfR) to efficient methane oxidation coupled to denitrification (AME-D)
Water Research,
Год журнала:
2024,
Номер
251, С. 121107 - 121107
Опубликована: Янв. 4, 2024
Язык: Английский
Polyhydroxyalkanoate Production by Methanotrophs: Recent Updates and Perspectives
Polymers,
Год журнала:
2024,
Номер
16(18), С. 2570 - 2570
Опубликована: Сен. 11, 2024
Methanotrophs
are
bacteria
that
consume
methane
(CH
Язык: Английский
Using RNA-Stable isotope probing to investigate methane oxidation metabolites and active microbial communities in methane oxidation coupled to denitrification
Chemosphere,
Год журнала:
2024,
Номер
357, С. 142067 - 142067
Опубликована: Апрель 20, 2024
The
active
denitrifying
communities
performing
methane
oxidation
coupled
to
denitrification
(MOD)
were
investigated
using
samples
from
an
aerobic
reactor
(∼
20%
O2
and
2%
CH4)
a
microaerobic
(2%
O2,
undertaking
denitrification.
metabolites
excreted
in
the
reactors
acetate,
methanol,
formate
acetaldehyde.
Using
anaerobic
batch
experiments
supplemented
with
exogenously
supplied
13C-labelled
metabolites,
bacteria
identified
16S
rRNA
amplicon
sequencing
RNA-stable
isotope
probing
(RNA-SIP).
With
(AR)
samples,
maximum
NO3-
removal
rates
0.43
mmol
g-1
d-1,
0.40
0.33
d-1
0.10
for
formate,
acetaldehyde
methanol
treatments
respectively,
while
(MR)
0.41
0.38
0.14
respectively.
RNA-SIP
Methyloversatilis,
Hyphomicrobium
as
methane-driven
AR
Pseudoxanthomonas,
Hydrogenophaga
MOD
MR
samples.
Collectively,
all
data
indicate
that
is
key
cross-feeding
metabolite
by
methanotrophs
consumed
denitrifiers
MOD.
Язык: Английский
Fluorescent protein-based anaerobic reporter for construction of promoter libraries in Clostridium autoethanogenum
International Journal of Biological Macromolecules,
Год журнала:
2025,
Номер
unknown, С. 143155 - 143155
Опубликована: Апрель 1, 2025
Язык: Английский
Implementation of an Upflow Fixed Bed Bioreactor for Denitrification Coupled to Methane Oxidation: Performance and Biomass Development Under Anoxic Conditions
Water Air & Soil Pollution,
Год журнала:
2024,
Номер
235(12)
Опубликована: Окт. 24, 2024
Abstract
Denitrification
coupled
to
methane
oxidation
(DOM)
has
been
shown
be
an
appropriate
process
for
wastewater
treatment
applications,
since
it
can
reduce
greenhouse
gas
emissions
and
nitrogen
discharges,
making
plants
more
environmentally
sustainable.
Study
of
DOM
focused
on
laboratory-scale
application
using
membrane
biological
reactors
(MBR)
or
sequency
batch
(SBR),
which
have
able
retain
biomass
therefore
use
with
this
process.
However,
is
necessary
expand
knowledge
the
behavior
other
configurations,
a
view
scaling
up.
Therefore,
in
study,
upflow
fixed
bed
bioreactor
(UFBR)
was
implemented
plastic
carriers
such
as
bioballs
Biochips®
carry
out
under
anoxic
conditions.
The
reactor
reached
stable
removal
conditions
after
approximately
400
days
continuous
operation,
forming
composed
denitrifying
methane-oxidizing
microorganisms
where
genus
Anaerolinea
Methylocystis
predominated.
Once
formed
stabilized,
maximum
nitrite
nitrate
rates
17.6
mgN-NO
2
−
/L-d
8.9
3
/L-d,
respectively,
efficiency
up
77%
were
obtained.
This
demonstrates
feasibility
bioreactors,
promising
further
from
varied
configuration
easily
scaled-up.
Язык: Английский
Using Rna-Stable Isotope Probing to Investigate Methane Oxidation Metabolites and Active Microbial Communities in Methane Oxidation Coupled to Denitrification
Опубликована: Янв. 1, 2024
The
active
denitrifying
communities
performing
methane
oxidation
coupled
to
denitrification
(MOD)
were
investigated
using
samples
from
an
aerobic
reactor
(2%
CH4,
98%
air)
and
a
microaerobic
O2,
2%
CH4
96%
N2)
undertaking
denitrification.
metabolites
excreted
in
the
reactors
acetate,
methanol,
formate
acetaldehyde.
Using
anaerobic
batch
experiments
supplemented
with
exogenously
supplied
13C-labelled
metabolites,
bacteria
identified
16S
rRNA
amplicon
sequencing
RNA-stable
isotope
probing
(RNA-SIP).
With
(AR)
samples,
maximum
NO3-
removal
rates
0.43
mmol
g-1
d‑1,
0.40
d-1,
0.33
d-1
0.10
for
formate,
acetaldehyde
methanol
treatments
respectively,
while
(MR)
0.41
38
0.14
respectively.
RNA-SIP
Methyloversatilis,
Hyphomicrobium
as
methane-driven
AR
Pseudoxanthomonas,
Hydrogenophaga
MOD
MR
samples.
Collectively,
all
data
indicate
that
is
key
cross-feeding
metabolite
by
methanotrophs
consumed
denitrifiers
MOD.
Язык: Английский
A Study of the Community Relationships Between Methanotrophs and Their Satellites Using Constraint-Based Modeling Approach
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(22), С. 12469 - 12469
Опубликована: Ноя. 20, 2024
Biotechnology
continues
to
drive
innovation
in
the
production
of
pharmaceuticals,
biofuels,
and
other
valuable
compounds,
leveraging
power
microbial
systems
for
enhanced
yield
sustainability.
Genome-scale
metabolic
(GSM)
modeling
has
become
an
essential
approach
this
field,
which
enables
a
guide
targeting
genetic
modifications
optimization
pathways
various
industrial
applications.
While
single-species
GSM
models
have
traditionally
been
employed
optimize
strains
like
Язык: Английский
Production of Bacillus subtillis protein mass on the microbial mass of Methylococcus capsulatus
Scientific Horizons,
Год журнала:
2023,
Номер
26(10), С. 127 - 137
Опубликована: Авг. 26, 2023
The
growing
demand
for
food
of
animal
origin
has
contributed
to
the
annual
increase
in
agricultural
population
productive
animals
world,
which
turn
led
a
significant
shortage
feeds
and
an
their
cost.
Therefore,
purpose
this
research
was
explore
qualitative
composition
feed
products
obtained
by
culturing
methanotrophic
microorganisms
Methylococcus
capsulatus
with
probiotic
bacteria
Bacillus
subtillis.
Arbitrage
methods
determining
quality
feeds,
are
regulated
regulatory
documents
(ISO),
were
used
research.
results
demonstrate
that
protein
product
culture
high
content
“crude”
at
level
60.9%,
corresponds
high-protein
its
technical
characteristics.
addition
subtillis
medium
cultivation
significantly
improved
parameters
synthesised
increasing
1.5%
amount
essential
amino
acids,
3.8%
fatty
acids
concentration
calcium
phosphorus.
lysine,
isoleucine,
valine
asparagic
acid,
due
cocultivation
two
allowed
nutritional
value
cattle
since
these
most
demanded
ruminant
diets
when
using
corn
silage
other
plant
feeds.
reducing
fibre
improving
biological
characteristics
changing
microbiological
product.
In
future,
biotechnological
method
obtaining
will
reduce
dependence
breeding
on
fodder
crops
weather
conditions
Язык: Английский