Management of biofilm by an innovative layer-structured membrane for membrane biofilm reactor (MBfR) to efficient methane oxidation coupled to denitrification (AME-D)

Water Research, Journal Year: 2024, Volume and Issue: 251, P. 121107 - 121107

Published: Jan. 4, 2024

Language: Английский

---

Polyhydroxyalkanoate Production by Methanotrophs: Recent Updates and Perspectives

Polymers, Journal Year: 2024, Volume and Issue: 16(18), P. 2570 - 2570

Published: Sept. 11, 2024

Methanotrophs are bacteria that consume methane (CH

Language: Английский

---

Fluorescent protein-based anaerobic reporter for construction of promoter libraries in Clostridium autoethanogenum

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 143155 - 143155

Published: April 1, 2025

Language: Английский

---

Using RNA-Stable isotope probing to investigate methane oxidation metabolites and active microbial communities in methane oxidation coupled to denitrification

Chemosphere, Journal Year: 2024, Volume and Issue: 357, P. 142067 - 142067

Published: April 20, 2024

The active denitrifying communities performing methane oxidation coupled to denitrification (MOD) were investigated using samples from an aerobic reactor (∼ 20% O2 and 2% CH4) a microaerobic (2% O2, undertaking denitrification. metabolites excreted in the reactors acetate, methanol, formate acetaldehyde. Using anaerobic batch experiments supplemented with exogenously supplied 13C-labelled metabolites, bacteria identified 16S rRNA amplicon sequencing RNA-stable isotope probing (RNA-SIP). With (AR) samples, maximum NO3- removal rates 0.43 mmol g-1 d-1, 0.40 0.33 d-1 0.10 for formate, acetaldehyde methanol treatments respectively, while (MR) 0.41 0.38 0.14 respectively. RNA-SIP Methyloversatilis, Hyphomicrobium as methane-driven AR Pseudoxanthomonas, Hydrogenophaga MOD MR samples. Collectively, all data indicate that is key cross-feeding metabolite by methanotrophs consumed denitrifiers MOD.

Language: Английский

---

Implementation of an Upflow Fixed Bed Bioreactor for Denitrification Coupled to Methane Oxidation: Performance and Biomass Development Under Anoxic Conditions

Water Air & Soil Pollution, Journal Year: 2024, Volume and Issue: 235(12)

Published: Oct. 24, 2024

Abstract Denitrification coupled to methane oxidation (DOM) has been shown be an appropriate process for wastewater treatment applications, since it can reduce greenhouse gas emissions and nitrogen discharges, making plants more environmentally sustainable. Study of DOM focused on laboratory-scale application using membrane biological reactors (MBR) or sequency batch (SBR), which have able retain biomass therefore use with this process. However, is necessary expand knowledge the behavior other configurations, a view scaling up. Therefore, in study, upflow fixed bed bioreactor (UFBR) was implemented plastic carriers such as bioballs Biochips® carry out under anoxic conditions. The reactor reached stable removal conditions after approximately 400 days continuous operation, forming composed denitrifying methane-oxidizing microorganisms where genus Anaerolinea Methylocystis predominated. Once formed stabilized, maximum nitrite nitrate rates 17.6 mgN-NO 2 − /L-d 8.9 3 /L-d, respectively, efficiency up 77% were obtained. This demonstrates feasibility bioreactors, promising further from varied configuration easily scaled-up.

Language: Английский

---

Using Rna-Stable Isotope Probing to Investigate Methane Oxidation Metabolites and Active Microbial Communities in Methane Oxidation Coupled to Denitrification

Published: Jan. 1, 2024

The active denitrifying communities performing methane oxidation coupled to denitrification (MOD) were investigated using samples from an aerobic reactor (2% CH4, 98% air) and a microaerobic O2, 2% CH4 96% N2) undertaking denitrification. metabolites excreted in the reactors acetate, methanol, formate acetaldehyde. Using anaerobic batch experiments supplemented with exogenously supplied 13C-labelled metabolites, bacteria identified 16S rRNA amplicon sequencing RNA-stable isotope probing (RNA-SIP). With (AR) samples, maximum NO3- removal rates 0.43 mmol g-1 d‑1, 0.40 d-1, 0.33 d-1 0.10 for formate, acetaldehyde methanol treatments respectively, while (MR) 0.41 38 0.14 respectively. RNA-SIP Methyloversatilis, Hyphomicrobium as methane-driven AR Pseudoxanthomonas, Hydrogenophaga MOD MR samples. Collectively, all data indicate that is key cross-feeding metabolite by methanotrophs consumed denitrifiers MOD.

Language: Английский

---

A Study of the Community Relationships Between Methanotrophs and Their Satellites Using Constraint-Based Modeling Approach

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(22), P. 12469 - 12469

Published: Nov. 20, 2024

Biotechnology continues to drive innovation in the production of pharmaceuticals, biofuels, and other valuable compounds, leveraging power microbial systems for enhanced yield sustainability. Genome-scale metabolic (GSM) modeling has become an essential approach this field, which enables a guide targeting genetic modifications optimization pathways various industrial applications. While single-species GSM models have traditionally been employed optimize strains like

Language: Английский

---

Production of Bacillus subtillis protein mass on the microbial mass of Methylococcus capsulatus

Scientific Horizons, Journal Year: 2023, Volume and Issue: 26(10), P. 127 - 137

Published: Aug. 26, 2023

The growing demand for food of animal origin has contributed to the annual increase in agricultural population productive animals world, which turn led a significant shortage feeds and an their cost. Therefore, purpose this research was explore qualitative composition feed products obtained by culturing methanotrophic microorganisms Methylococcus capsulatus with probiotic bacteria Bacillus subtillis. Arbitrage methods determining quality feeds, are regulated regulatory documents (ISO), were used research. results demonstrate that protein product culture high content “crude” at level 60.9%, corresponds high-protein its technical characteristics. addition subtillis medium cultivation significantly improved parameters synthesised increasing 1.5% amount essential amino acids, 3.8% fatty acids concentration calcium phosphorus. lysine, isoleucine, valine asparagic acid, due cocultivation two allowed nutritional value cattle since these most demanded ruminant diets when using corn silage other plant feeds. reducing fibre improving biological characteristics changing microbiological product. In future, biotechnological method obtaining will reduce dependence breeding on fodder crops weather conditions

Language: Английский

---