International Journal of Food Microbiology,
Год журнала:
2024,
Номер
425, С. 110872 - 110872
Опубликована: Авг. 15, 2024
This
study
aimed
to
determine
the
compatibility
of
pre-fermented
sugar
beet
pulp
support
growth
Pleurotus
ostreatus
mycelium
in
submerged
fermentation.
The
goal
was
create
a
meat
alternative
based
on
mycelial-fermented
pulp.
It
further
explored
whether
pre-fermentation
with
lactic
acid
bacteria
(LAB)
increased
meat-like
properties,
such
as
aroma,
springiness,
and
hardness,
final
product.
Three
strains
were
selected
from
high
throughput
screening
105
plant-derived
LAB
their
acidification
metabolite
production
Two
homofermentative
(Lactococcus
lactis)
one
heterofermentative
strain
(Levilactobacillus
brevis)
low
ethanol
production,
overall
Mycelium
P.
grown
fermentations
pulp,
biomass
removed
by
centrifugation.
fungal
consumed
all
available
sugars
acids
released
arabinose
media.
Volatiles
detected
using
GC–MS,
large
increase
concentrations
hexanal,
1-octen-3-ol,
2-octenal
measured.
Concentration
1-octen-3-ol
lower
samples
vs.
non-pre-fermented.
LC-MS
amino
analysis
showed
presence
essential
day
0
7
highest
concentration
for
glutamic
acid/glutamine
aspartic
acid/asparagine.
A
decrease
after
days
fermentation
measured
fermentations.
more
significant
samples.
also
confirmed
through
total
protein
determination,
except
Lactococcus
lactis
NFICC142
which
content
digestibility
fermentation,
seen
non-pre-fermented
springiness
fermented
product
indicated
similarities
alternatives,
while
hardness
much
than
other
alternatives.
results
indicate
that
dried
can
be
used
cultivation
ostreatus,
but
does
not
improve
physical
or
nutritional
properties
end
significantly,
an
is
first
known
attempt
use
mixed
produce
mycelium,
well
at
SBP
liquid
mycelial
ostreatus.
Foods,
Год журнала:
2023,
Номер
12(12), С. 2295 - 2295
Опубликована: Июнь 7, 2023
The
demand
for
cheap,
healthy,
and
sustainable
alternative
protein
sources
has
turned
research
interest
into
microbial
proteins.
Mycoproteins
prevail
due
to
their
quite
balanced
amino
acid
profile,
low
carbon
footprint
high
sustainability
potential.
goal
of
this
was
investigate
the
capability
Pleurotus
ostreatus
metabolize
main
sugars
agro-industrial
side
streams,
such
as
aspen
wood
chips
hydrolysate,
produce
high-value
with
cost.
Our
results
indicate
that
P.
LGAM
1123
could
be
cultivated
both
in
a
C-6
(glucose)-
C-5(xylose)-sugar-containing
medium
mycoprotein
production.
A
mixture
glucose
xylose
found
ideal
biomass
production
content
rich
profile.
cultivation
4
L
stirred-tank
bioreactor
using
hydrolysate
achieved
25.0
±
3.4
g
L−1
production,
1.8
0.4
d−1
specific
growth
rate
yield
54.5
0.5%
(g/100
sugars).
PCA
analysis
acids
revealed
strong
correlation
between
composition
produced
ratios
culture
medium.
high-nutrient
by
submerged
fermentation
edible
fungus
hydrolysates
is
promising
bioprocess
food
feed
industry.
Sustainable Food Technology,
Год журнала:
2023,
Номер
2(1), С. 81 - 91
Опубликована: Ноя. 17, 2023
Mycoprotein,
a
meat
alternative,
can
be
produced
using
submerged
fermentation,
solid-state
or
surface
culture
method.
It
is
an
eco-friendly
alternative
with
impressive
health
benefits.
Abstract
Background
The
production
of
Pleurotus
ostreatus
mycelium
as
a
promising
object
for
use
in
food
and
other
industries
is
hampered
by
lack
information
about
the
strain-specificity
this
fungus
growth
its
acquisition
various
biological
activities.
Therefore,
research
aimed
to
investigate
mycelial
different
P.
strains
on
varies
solid
liquid
media
well
evaluate
antagonistic,
antibacterial,
antiradical
scavenging
activities,
total
phenolic
content.
Results
Potato
Dextrose
Agar
medium
was
suitable
all
except
strain
2460.
best
rate
2462
culture
15.0
±
0.8
mm/day,
mycelia
media—36.5
0.2
g/l.
551
1685
were
more
susceptible
positive
effect
plant
regulators
Ivin,
Methyur
Kamethur.
Using
nutrient
based
combination
natural
waste
(amaranth
flour
cake
wheat
germ,
bran,
broken
vermicelli
crumbs)
has
been
increased
yield
2.2–2.9
times
compared
control.
All
used
displayed
strong
antagonistic
activity
co-cultivation
with
Aspergillus
niger
,
Candida
albicans
Issatchenkia
orientalis
Fusarium
poae
Microdochium
nivale
dual-culture
assay.
EtOAc
extract
good
inhibited
Escherichia
coli
(17.0
0.9
mm)
while
2460
suppressed
Staphylococcus
aureus
(21.5
0.5
agar
diffusion
method.
highest
radical
both
extracts
(EtOH
EtOAc)
(61
56%)
DPPH
assay
high
content
(7.17
6.73
mg
GAE/g)
Folin-Ciocalteu’s
maximal
phenol
(7.52
demonstrated
2461
EtOH
extract.
Conclusions
It
found
that
growth,
dependent
studied
contrast
activity.
proposed
mediums
could
be
an
alternative
commercial
biomass
strains.
Journal of Fungi,
Год журнала:
2024,
Номер
10(3), С. 183 - 183
Опубликована: Фев. 28, 2024
Modern
efforts
to
influence
materials
science
with
principles
of
biology
have
allowed
fungal
mycelial
take
a
foothold
and
develop
novel
solutions
for
the
circular
bioeconomy
tomorrow.
However,
recent
studies
shown
that
value
tomorrow’s
green
is
not
determined
simply
by
their
environmental
viability,
but
rather
ability
make
polluting
today
obsolete.
With
an
inherently
strong
structure
chitin
β-glucan,
ever-adaptable
mycelia
fungi
can
compete
at
highest
levels
litany
from
leather
polyurethane
foam
paper
wood.
There
are
significant
optimize
pure
(PMMs)
through
entire
process
species
strain
selection,
growth,
fabrication.
Indeed,
promising
investigations
demonstrate
how
diversity
be
leveraged
create
uniquely
specialized
materials.
This
review
aims
highlight
PMMs’
current
trajectory,
evaluate
successes
in
technology,
explore
these
new
help
shape
better
Molecules,
Год журнала:
2025,
Номер
30(1), С. 165 - 165
Опубликована: Янв. 3, 2025
Seaweed
biomass
is
globally
underutilized
as
a
source
of
proteins
despite
its
nutritional
potential,
with
much
use
focused
on
hydrocolloid
extraction.
This
study
evaluated
the
quality
and
digestibility
protein
amino
acids
from
two
brown
seaweeds
(Durvillaea
spp.
Macrocystis
pyrifera),
one
green
seaweed
(Ulva
spp.),
novel
mycoprotein
derived
Durvillaea
through
fungal
fermentation.
Using
an
in
vitro
gastrointestinal
digestion
Megazyme
assay
kit,
digestibility-corrected
acid
scores
(PDCAASs)
digestible
indispensable
(DIASSs)
were
determined.
Compared
seaweeds,
seaweed-derived
presented
significantly
greater
contents
(~33%)
profiles
(2.2
times
than
those
M.
(~100%)
(<60%).
The
PDCAAS
values
0.37,
0.41,
0.53,
0.89
for
Ulva
spp.,
pyrifera,
mycoproteins,
respectively.
DIASSs
highlighted
superior
mycoprotein,
particularly
lysine
(0.59)
histidine
(0.67).
SDS-PAGE
revealed
soluble
peptides
(<25
kDa)
whereas
exhibited
limited
solubility
due
to
structural
aggregation.
These
findings
highlight
need
characterize
properties
edible
Chile
further
emphasize
importance
optimized
processing
techniques,
such
fermentation
or
bioconversion,
improve
potential
develop
high-quality
food
ingredients
diverse
applications.
Applied Microbiology and Biotechnology,
Год журнала:
2025,
Номер
109(1)
Опубликована: Фев. 10, 2025
Abstract
Filamentous
fungi
or
mycelia
are
a
valuable
bioresource
to
produce
several
biomolecules
and
enzymes,
especially
because
of
their
biodegradation
potential
for
key
role
enablers
circular
bioeconomy.
can
be
grown
in
submerged
cultivation
maximise
the
volumetric
productivity
bioprocess,
instead
using
more
established
time-consuming
solid-state
cultivation.
Multicellular
sensitive
shear
stresses
induced
by
mechanical
agitation,
this
aspect
greatly
affects
morphology
(pelletisation)
connected
productivity.
An
efficient
compromise
is
growth
filamentous
airlift
bioreactors
(ALR)
where
oxygen
transfer
(K
L
a)
optimal,
but
stress
reduced.
In
review,
we
critically
analysed
advantages
disadvantages
ALR-based
fungi,
comparing
these
also
with
stirred
tank
reactors
bubble
column
reactors;
focused
on
scientific
literature
that
highlights
findings
both
production
enzymes
myco-biomass
ALR;
included
studies
control
pelletisation
fungal
biomass
batch
semi-continuous
cultivation,
highlighting
interlinked
hydrodynamics;
finally,
regarding
modifications
ALR
order
enhance
production.
Key
points
•
prolonged
continuous
fungi.
show
optimal
gas
hold-up
K
an
airflow
has
high
superficial
velocity
critical
diameter
(1–6
mm).
Suspended
aggregates
(pellet)
maintain
fluidised
motion
if
size/density
controlled.
