Persistence of microbiological hazards in food and feed production and processing environments

EFSA Journal, Год журнала: 2024, Номер 22(1)

Опубликована: Янв. 1, 2024

Abstract Listeria monocytogenes (in the meat, fish and seafood, dairy fruit vegetable sectors), Salmonella enterica feed, egg low moisture food sectors) Cronobacter sakazakii sector) were identified as bacterial safety hazards most relevant to public health that are associated with persistence in feed processing environment (FFPE). There is a wide range of subtypes these involved FFPE. While some specific more commonly reported persistent, it currently not possible identify universal markers (i.e. genetic determinants) for this trait. Common risk factors FFPE inadequate zoning hygiene barriers; lack hygienic design equipment machines; cleaning disinfection. A well-designed environmental sampling testing programme effective strategy contamination sources detect potentially persistent hazards. The establishment barriers measures within management system, during implementation hazard analysis critical control points, key prevent and/or Once suspected plant, 'seek-and-destroy' approach frequently recommended, including intensified monitoring, introduction continuation monitoring. Successful actions triggered by L. described, well interventions direct bactericidal activity. These could be efficient if properly validated, correctly applied verified under industrial conditions. Perspectives provided performing assessment combinations sector assess relative can persistence, based on bottom-up top-down approaches. Knowledge gaps related priorities future research provided.

Язык: Английский

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Anti-biofilm mechanisms of action of essential oils by targeting genes involved in quorum sensing, motility, adhesion, and virulence: A review

International Journal of Food Microbiology, Год журнала: 2024, Номер 426, С. 110874 - 110874

Опубликована: Авг. 22, 2024

Biofilms are a critical factor for food safety, causing important economic losses. Among the novel strategies controlling biofilms, essential oils (EOs) can represent an environmentally friendly approach, able to act both on early and mature stages of biofilm formation. This review reports anti-biofilm mechanisms action EOs against five pathogenic bacterial species known their biofilm-forming ability. These include disturbing expression genes related quorum sensing (QS), motility, adhesion, virulence. QS interconnected processes, interfere with communication system (e.g. regulating agrBDCA, luxR, luxS, pqsA genes), thus influencing In addition, is mechanism that regulates gene survival, virulence, pathogenicity. Similarly, also influence many virulence genes. Moreover, exert effects modulating associated adhesion example those involved in curli (csg), fimbriae (fim, lpf), flagella (fla, fli, flh, mot) production, as well ica responsible synthetizing polysaccharide intercellular adhesin. provides comprehensive framework topic better understanding action.

Язык: Английский

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Spent coffee ground disrupts Listeria monocytogenes biofilm formation through inhibition of motility and adhesion via quorum sensing regulation

International Journal of Food Microbiology, Год журнала: 2025, Номер 430, С. 111066 - 111066

Опубликована: Янв. 14, 2025

Язык: Английский

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Outbreak of listeriosis associated with consumption of deli meats in a hospital, Germany, February to March 2023

Eurosurveillance, Год журнала: 2025, Номер 30(7)

Опубликована: Фев. 20, 2025

Listeria monocytogenes can cause severe illness in individuals with weakened immune systems. In March 2023, L. was isolated from blood (n = 2) or pleural fluid 1) of three febrile patients receiving synthetic glucocorticoids a tertiary hospital Germany. Food supply records suggested sliced parboiled sausage as the likely source, and four samples sealed packaged sausages ham one manufacturer. The patient food isolates clustered within 0–4 allelic differences. Counts all were < 100 colony-forming units (CFU)/g, threshold European Union legislation for ready-to-eat products specific conditions. Our findings, aligned previous evidence, highlight that persons systems should not be exposed to food. We advocate clear communication deli meats high-risk foods, so adjust their diet reduce risk invasive listeriosis. recommend an update dietary hygiene guidelines care settings private homes where is prepared, handled stored

Язык: Английский

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Listeria monocytogenes and Listeriosis: The Global Enigma

Foods, Год журнала: 2025, Номер 14(7), С. 1266 - 1266

Опубликована: Апрель 3, 2025

Listeria monocytogenes is an intracellular, Gram-positive, non-spore-forming, non-encapsulated, facultative anaerobic, rod-shaped, and psychrotrophic food-borne pathogen that causes the infection, listeriosis, thus it attracts great attention following listeriosis outbreaks, which are often associated with high mortality rates. The prevalence of quite low globally; however, most recent deadliest outbreak occurred in South Africa, during 216 persons lost their lives. L. endowed potential to multiply through a wide range harsh environmental conditions, forming biofilms on varying surfaces food industry, as well having persistent antibiotic-resistant cells, pose major threat burden ready-to-eat industry. A more frustrating characteristic this bacterium its strain divergence, alongside increased level antibiotic resistance registered among strains recovered from food, humans, sources, especially those antibiotics involved treatment human listeriosis. Antibiotic exerted by pathogenic microbes ongoing public health menace continues be issue. Against background, thorough search into different databases using various engines was performed, led gathering salient information organised, chronologically, based Altogether, findings elaborated study present up-to date knowledge aspects will improve our understanding mystery ways prevent control dissemination foods. In addition, constant monitoring profiles sources detected changes, giving update trend resistance. Overall, bacterial contamination serves key aspect safety output

Язык: Английский

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Exploring the occurrence of Listeria in biofilms and deciphering the bacterial community in a frozen vegetable producing environment

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Июль 10, 2024

The establishment of Listeria (L.) monocytogenes within food processing environments constitutes a significant public health concern. This versatile bacterium demonstrates an exceptional capacity to endure challenging environmental conditions in the environment, where contamination products regularly occurs. diverse repertoire stress resistance genes, potential colonize biofilms, and support co-existing microbiota have been proposed as root causes for survival L. environments. In this study, 71 sites were sampled after cleaning disinfection European frozen vegetable facility, in-house clones persisted years. innocua detected by culture-dependent method at 14 sampling sites, primarily on conveyor belts associated parts. presence determined quantification bacterial load analysis extracellular matrix components (carbohydrates, proteins, DNA) was confirmed nine (12.7%). two cases, biofilm. Furthermore, we explored resident microbial community environment biofilm-positive well co-occurrence taxa with 16S rRNA gene sequencing. Pseudomonas , Acinetobacter Exiguobacterium dominated environment. Using differential abundance analysis, amplicon sequence variants (ASVs) assigned Enterobacterales ( Enterobacter Serratia unclassified Enterobacteriaceae ) Carnobacterium found be significantly higher abundant -positive samples. Several ASVs less compared -negative Acinetobacter, Janthinobacterium Brevundimonas key players more relatively biofilms. Further, composition varied between different areas surface materials.

Язык: Английский

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Airborne signals of Pseudomonas fluorescens modulate swimming motility and biofilm formation of Listeria monocytogenes in a contactless coculture system

Food Microbiology, Год журнала: 2024, Номер 120, С. 104494 - 104494

Опубликована: Фев. 15, 2024

Язык: Английский

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Antimicrobial resistance and prevalence of Listeria species from raw milk and dairy products in Burdur, Turkey

Veterinary Medicine and Science, Год журнала: 2024, Номер 10(5)

Опубликована: Июль 25, 2024

Worldwide, but especially in emerging nations, concerns about food safety pose a serious obstacle to societal and economic progress. This research aimed examine the prevalence of Listeria spp. raw milk dairy products Burdur, as well presence genes associated with biofilm formation antibiotic resistance isolates.

Язык: Английский

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Prevalence of Campylobacter spp., Salmonella spp., and Listeria monocytogenes, and Population Levels of Food Safety Indicator Microorganisms in Retail Raw Chicken Meat and Ready-To-Eat Fresh Leafy Greens Salads Sold in Greece

Foods, Год журнала: 2023, Номер 12(24), С. 4502 - 4502

Опубликована: Дек. 16, 2023

The presence of microbial pathogens in foods compromises their safety resulting foodborne illnesses, public health disorders, product recalls, and economic losses. In this work, 60 samples chilled raw chicken meat 40 packaged ready-to-eat (RTE) fresh leafy greens salads, sold Greek retail stores (butchers supermarkets), were analyzed for the three important pathogenic bacteria, i.e., Campylobacter spp., Salmonella Listeria monocytogenes, following detection protocols International Organization Standardization (ISO). parallel, total aerobic plate count (APC), Enterobacteriaceae, coliforms, Escherichia coli, staphylococci also enumerated as hygiene (safety) indicator organisms. When present, representative typical colonies each pathogen biochemically verified, ISO guidelines. At same time, all isolates from (n = 120) identified to species level further phylogenetically discriminated through multiplex repetitive sequence-based (rep) polymerase chain reaction (PCR) methods, respectively. Concerning chicken, spp. recovered 54 (90.0%) 9 (15.0%), while L. monocytogenes was present 35 (58.3%). No only one sample (2.5%), salads found be contaminated with (7.5%). 65% belonged C. jejuni, whereas rest, 35%, coli. Alarmingly, APC equal or above 106 CFU/g 53.3% 95.0% salad samples, respectively, populations some other indicators cases high. sum, study unravels high occurrence percentages food microorganisms RTE retail, highlighting need more extensive microbiological control throughout production (from farm/field market).

Язык: Английский

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The Relationship between Biofilm Phenotypes and Biofilm-Associated Genes in Food-Related Listeria monocytogenes Strains

Microorganisms, Год журнала: 2024, Номер 12(7), С. 1297 - 1297

Опубликована: Июнь 26, 2024

is an important pathogen responsible for listeriosis, a serious foodborne illness associated with high mortality rates. Therefore,

Язык: Английский

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