medRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Май 26, 2023
Abstract
Dry
eye
disease
(DED)
affects
up
to
half
of
the
world’s
population
and
causes
blur,
pain,
decreased
quality
life.
Unfortunately,
many
people
do
not
receive
proper
treatment
due
current
challenges
associated
with
DED
diagnoses.
To
address
this
issue,
point-of-care
(PoC)
clinical
tear
film
diagnostics
are
beginning
be
used
for
rapid
in-office
testing.
However,
these
also
have
significant
obstacles,
including
sampling.
We
previously
demonstrated
how
soft
contact
lenses
(SCLs)
can
capture
concentrate
proteins
on
eye.
Given
low
volume
subjects,
SCL
sampling
holds
great
potential
improve
PoC
facilitate
prompt
patients.
Here
we
show
detection
new
established
protein
biomarkers
using
our
method.
Our
analysis
revealed
associations
between
immune
system,
ferroptosis,
salivary
secretion,
calcium
binding
proteins.
identified
correlations
testing
proteomics,
emphasizing
power
importance
translational
medicine
research.
These
experiments
lay
foundation
both
sampled
biomarker
discovery
as
well
improved
diagnostic
patient
care.
Figure
British Journal of Pharmacology,
Год журнала:
2024,
Номер
181(21), С. 4245 - 4261
Опубликована: Июль 8, 2024
This
study
investigated
the
involvement
of
discoidin
domain
receptor
(DDR)
in
dry
eye
and
assessed
potential
specific
DDR
inhibitors
as
a
therapeutic
strategy
for
by
exploring
underlying
mechanism.
Bioengineering,
Год журнала:
2024,
Номер
11(10), С. 1010 - 1010
Опубликована: Окт. 11, 2024
Ocular
surface
disease
(OSD)
is
a
frequent
disabling
challenge
among
patients
with
glaucoma
who
use
benzalkonium
chloride
(BAK)-containing
topical
medications
for
prolonged
periods.
In
this
comprehensive
review,
we
evaluated
the
prevalence
of
OSD
and
its
management,
focusing
on
both
current
future
alternatives.
Preferred
Reporting
Items
Systematic
Reviews
Meta-Analyses
(PRISMA)
criteria
were
used
to
assess
a)
impact
active
ingredients
preservatives
ocular
b)
efficacy
preservative-free
(PF)
alternatives
adjunctive
therapies.
BAK-containing
found
significantly
contribute
by
increasing
corneal
staining,
reducing
tear
film
stability,
elevating
index
(OSDI)
scores.
Transitioning
PF
formulations
or
those
less
cytotoxic
preservatives,
such
as
Polyquad
Journal of Inflammation Research,
Год журнала:
2024,
Номер
Volume 17, С. 10769 - 10784
Опубликована: Дек. 1, 2024
Run-Mu-Ling
granules
(RMLG),
a
traditional
Chinese
medicinal
formula,
are
used
to
treat
dry
eye
(DE);
however,
the
underlying
mechanism
remains
poorly
understood.
This
study
aimed
elucidate
potential
molecular
mechanisms
by
which
RMLG
alleviates
ocular
surface
inflammation
in
DE.
Frontiers in Pharmacology,
Год журнала:
2024,
Номер
15
Опубликована: Сен. 11, 2024
The
prevalence
of
dry
eye
disease
(DED),
a
multifactorial
ocular
surface
characterized
by
tear
film
instability,
is
increasing
yearly.
Qingxuan
Run
Mu
Yin
(QXRMY)
traditional
Chinese
medicine
(TCM)
consisting
Scientific Reports,
Год журнала:
2023,
Номер
13(1)
Опубликована: Июль 27, 2023
Abstract
Dry
eye
disease
(DED)
is
a
challenge
in
ophthalmology.
Rat
models
represent
valuable
tools
to
study
the
pathophysiology
and
develop
novel
treatments.
A
major
DED
research
detecting
multiple
biomarkers
low
tear
volume
sample.
Multiplex
immunoassays
for
rat
are
missing.
We
have
developed
multiplex
electrochemiluminescence
immunoassay
(ECLIA)
detect
three
DED:
MMP-9,
IL-17
ICAM-1.
Tears,
used
as
matrix,
were
collected
from
six
healthy
Wistar
rats.
Assays
run
based
on
U-Plex
Meso
Scale
Diagnostics
(MSD)
platform,
by
two
independent
operators
according
EMA
guideline
bioanalytical
method
validation.
Linear
mixed,
regression
fit
perform
statistical
analysis
range
of
concentrations
chosen
analytes.
During
optimization,
it
has
observed
that
incubation
time,
temperature
agitation
affected
robustness
protocol.
ECLIA
optimum
conditions
include
use
antibodies
at
0.5
µg/ml
concentration
1
h
room
with
shaking.
Precision
met
acceptance
criteria
range:
1062–133
pg/ml
ICAM-1,
275–34.4
IL-17,
1750–219
MMP-9.
Accuracy
linearity
acceptable
broader
range.
This
first
report
validated
allows
measurements
relevant
fluids.
Nutrients,
Год журнала:
2023,
Номер
15(24), С. 5063 - 5063
Опубликована: Дек. 11, 2023
Dry
eye
disease
(DED)
occurs
when
there
are
not
enough
tears,
and
the
associated
symptoms-burns,
itching,
a
gritty
feeling
in
eye-can
cause
great
discomfort.
The
purpose
of
this
study
was
to
evaluate
therapeutic
effect
purple
corn
extract
(PCE)
on
DED.
Pretreatment
with
PCE
prevented
desiccation-stress-induced
cell
damage
human
retinal
pigment
epithelial
cells
primary
corneal
cells.
Furthermore,
reduced
mRNA
expression
inflammatory
mediators
induction
desiccation
stress.
effects
DED
were
evaluated
an
animal
model
induced
unilateral
excision
exorbital
lacrimal
gland.
administration
effective
at
recovering
tear
production,
surface
irregularity,
conjunctival
goblet
density,
as
well
reducing
apoptotic
death
outer
layer
epithelium.
Collectively,
improved
dry
symptoms,
and,
therefore,
it
could
be
potential
agent
ameliorate
and/or
treat
medRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Май 26, 2023
Abstract
Dry
eye
disease
(DED)
affects
up
to
half
of
the
world’s
population
and
causes
blur,
pain,
decreased
quality
life.
Unfortunately,
many
people
do
not
receive
proper
treatment
due
current
challenges
associated
with
DED
diagnoses.
To
address
this
issue,
point-of-care
(PoC)
clinical
tear
film
diagnostics
are
beginning
be
used
for
rapid
in-office
testing.
However,
these
also
have
significant
obstacles,
including
sampling.
We
previously
demonstrated
how
soft
contact
lenses
(SCLs)
can
capture
concentrate
proteins
on
eye.
Given
low
volume
subjects,
SCL
sampling
holds
great
potential
improve
PoC
facilitate
prompt
patients.
Here
we
show
detection
new
established
protein
biomarkers
using
our
method.
Our
analysis
revealed
associations
between
immune
system,
ferroptosis,
salivary
secretion,
calcium
binding
proteins.
identified
correlations
testing
proteomics,
emphasizing
power
importance
translational
medicine
research.
These
experiments
lay
foundation
both
sampled
biomarker
discovery
as
well
improved
diagnostic
patient
care.
Figure
