Review on the potential roles of traditional Chinese medicines in the prevention, treatment, and postoperative recovery of age-related cataract

Journal of Ethnopharmacology, Год журнала: 2024, Номер 324, С. 117786 - 117786

Опубликована: Янв. 21, 2024

Язык: Английский

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Fluorescence Imaging of Diabetic Cataract-Associated Lipid Droplets in Living Cells and Patient-Derived Tissues

ACS Sensors, Год журнала: 2023, Номер 8(10), С. 3882 - 3891

Опубликована: Сен. 22, 2023

Diabetic cataract (DC) surgery carries risks such as slow wound healing, macular edema, and progression of retinopathy is faced with a deficiency effective drugs. In this context, we proposed protocol to evaluate the drug's efficacy using lipid droplets (LDs) marker. For purpose, fluorescent probe PTZ-LD for LDs detection developed based on phenothiazine unit. The displays polarity-dependent emission variations, i.e., lower polarity leading stronger intensity. Especially, exhibits photostability superior that Nile Red, commercial staining dye. Using probe, formation in DC-modeled human lens epithelial (HLE) cells validated, interplay LDs-LDs LDs-others are investigated. Unexpectedly, transfer between visualized. Moreover, therapeutic various drugs HLE assessed. Ultimately, more were found tissues from DC patients than first time. We anticipate work can attract attention important roles during progression.

Язык: Английский

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Fosfenopril Attenuates Inflammatory Response in Diabetic Dry Eye Models by Inhibiting the TLR4/NF-κB/NLRP3 Signaling Pathway

Investigative Ophthalmology & Visual Science, Год журнала: 2024, Номер 65(6), С. 2 - 2

Опубликована: Июнь 3, 2024

Purpose: The purpose of this study was to investigate the involvement TLR4/NF-κB/NLRP3 signaling pathway and its underlying mechanism in diabetic dry eye. Methods: Two models eye were established high glucose-induced human corneal epithelial (HCE-T) cells streptozotocin (STZ)-induced C57BL/6 mice, TLR4 inhibitor fosfenopril (FOS) utilized suppress pathway. expression changes TLR4, NF-κB, NLRP3, IL-1β, other factors detected by Western blot RT‒qPCR, wound healing rate evaluated cell scratch assay, symptoms mice sodium fluorescein staining tear secretion assay. Results: In model, transcript levels IL-1β raised, further application FOS, a inhibitor, downregulated these factors. addition, FOS found be effective increasing HCE-T cells, production, decreasing fluorescence scores as measured production. Conclusions: current that regulates an vitro vivo reduces signs providing new treatment option for

Язык: Английский

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Effect of metformin on the level of aqueous humor inflammatory cytokines in patients with cataract

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Янв. 29, 2025

This study investigated the content of inflammatory cytokines in aqueous humor (AH) cataract patients with type 2 diabetes (T2DM) and explored effect metformin on level cytokines. AH was collected from undergoing phacoemulsification intraocular lens implantation Peking University Third Hospital. Levels were measured by Cytometric Bead Assay (CBA) Flex Set. Differences compared between using non-metformin medicine as blood sugar control drug for T2DM age-related without T2DM. A total 67 included, including 19 healthy controls, 33 group, 15 group. The results showed that IL-6 levels significantly higher group than (p = 0.019 0.014, respectively). IFN-γ also 0.031 0.003, IL-10 those 0.008), whereas no significant difference controls. Metformin can reduce to a certain extent combined It is suggested may have preventive therapeutic effects development cataract.

Язык: Английский

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Effect of different incisions on dry eye symptoms after cataract surgery in diabetic patients

BMC Ophthalmology, Год журнала: 2025, Номер 25(1)

Опубликована: Фев. 13, 2025

Язык: Английский

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Diabetic Macular Edema in the Western Part of Romania: Screening to Improve Patient Outcomes

Journal of Personalized Medicine, Год журнала: 2025, Номер 15(3), С. 106 - 106

Опубликована: Март 9, 2025

Background: Diabetes mellitus (DM) is a global healthcare concern with rising prevalence. Patients DM have severely diminished quality of life due to the extensive range connected complications. One most impactful diabetes-associated pathologies diabetic macular edema (DME), as it major cause blindness globally. DME present many concomitant diseases that influence their prognosis. The research seeks describe frequent DME-related comorbidities. Method: This study enrolled 105 participants previously diagnosed type 1 (T1DM) or 2 (T2DM) (77 presenting DME), who were evaluated regarding other associated Results: in group presented median age 65, mean disease duration 15 years and inadequate glycemic control, reflected by HbA1c 7.5%. All patients at least one comorbidity, hypertension (100%) dyslipidemia (62.3%) being prevalent. Spearman analysis revealed statistically significant correlation between diabetes (p = 0.01), proliferative retinopathy 0.004), chronic kidney 0.034). Conclusions: often multiple comorbidities must be screened for addressed through multidisciplinary approach.

Язык: Английский

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Therapeutic Effects of Dendrobium Extract on Pathologic Lens Alterations in Diabetic Cataract Patients

Pharmacognosy Magazine, Год журнала: 2025, Номер unknown

Опубликована: Март 25, 2025

Background Diabetic cataract, an ocular complication arising from diabetes, can lead to damage tissues, particularly affecting the lens and causing symptoms such as blurred vision. Purpose This work aimed demonstrate therapeutic effects of Dendrobium extract (DE) on pathogenic alterations in diabetic cataract patients comprehensively assess its vitro clinical research outcomes. Methods Initially, a cell model was established using high glucose (HG)-induced human epithelial cells (HLEB3). The were categorized into three groups: untreated control (UC) group, HG treatment HG+DE group. Apoptosis status, reactive oxygen species (ROS), superoxide dismutase (SOD), well Bcl-2, Bax, caspase-3 within cells, comparatively analyzed evaluate impact DE cells. Subsequently, cohort 60 recruited randomly allocated Group C (control 30 cases), which received conventional ophthalmic medications blood control, E (experimental intervention addition regimen. A comparative assessment visual acuity, intraocular pressure (IOP), grading before after conducted between two groups efficacy patient lenses. Results In study, group exhibited drastically inferior apoptotic rate compared (.05). production ROS inferior, SOD levels markedly superior those ( p < .05). showed greater decrease Bcl-2 protein increase Bax versus UC Moreover, displayed notably elevated greatly reduced vivo study demonstrated that improved acuity recovery .05), along with more stable IOP. remarkably Conclusion may contribute ameliorating lesions patients, mitigating damage, could potentially emerge promising option.

Язык: Английский

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Oxidation-Induced Mixed Disulfide and Cataract Formation: A Review

Antioxidants, Год журнала: 2025, Номер 14(4), С. 425 - 425

Опубликована: Апрель 1, 2025

The major function of eye lens is to transmit light onto retina and form an image. This relies on the crystallin proteins, which are tightly packed achieve a high refractive index transparency. proteins protected maintained in reduced state with intrinsic antioxidants, such as glutathione (GSH), redox-regulating enzyme systems, thioltransferase maintain SH/-S-S-balance. When protective systems impaired or due aging, oxidative stress can lead SH/S-S imbalance, protein modification, protein–protein aggregation loss transparency (cataract). Oxidative considered culprit senile cataract formation since cataractous lenses typically low GSH content have elevated levels GS-protein mixed disulfide (PSSG). review will examine PSSG accumulation age cataracts explore possible role oxidants H2O2. It also discuss hypothesis that not simply consequence but trigger cascade events leading supported by findings more rapid increasing weaker TTase activity and, animal model when gene deleted.

Язык: Английский

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Emodin alleviates the damage to lens epithelial cells in diabetic cataract by repressing the p53-mediated ferroptosis pathway

International Ophthalmology, Год журнала: 2025, Номер 45(1)

Опубликована: Апрель 2, 2025

Язык: Английский

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Cholesterol Content Regulates the Interaction of αA-, αB-, and α-Crystallin with the Model of Human Lens-Lipid Membranes

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(3), С. 1923 - 1923

Опубликована: Фев. 5, 2024

α-Crystallin (αABc) is a major protein comprised of αA-crystallin (αAc) and αB-crystallin (αBc) that found in the human eye lens works as molecular chaperone by preventing aggregation proteins providing tolerance to stress. However, with age cataract formation, concentration αABc cytoplasm decreases, corresponding increase membrane-bound αABc. This study uses electron paramagnetic resonance (EPR) spin-labeling method investigate role cholesterol (Chol) Chol bilayer domains (CBDs) binding αAc, αBc, Chol/model lens-lipid (Chol/MHLL) membranes. The maximum percentage membrane surface occupied (MMSO) Chol/MHLL membranes at mixing ratio 0 followed trends: MMSO > ≈ (αABc), indicating higher amount αAc binds these compared αBc an membranes, decreases until it completely diminished 1.5. Ka trend: (αAc), but was close zero mobility near headgroup regions decreased binding, antagonized capacity decrease regions. No significant change order observed, concentrations. Our results show bind differently ratios 0.5, decreasing increasing hydrophobicity region, likely forming hydrophobic barrier for passage polar ionic molecules, including antioxidants (glutathione), creating oxidative environment inside lens, leading development cataracts. all 1.5, high CBDs inhibit formation barriers protecting against formation.

Язык: Английский

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