Natural aporphine alkaloids: A comprehensive review of phytochemistry, pharmacokinetics, anticancer activities, and clinical application
Jing Sun,
Xingtian Zhan,
Weimin Wang
и другие.
Journal of Advanced Research,
Год журнала:
2023,
Номер
63, С. 231 - 253
Опубликована: Ноя. 5, 2023
Cancer
is
the
most
common
cause
of
death
and
still
a
serious
public
health
problem.
Alkaloids,
class
bioactive
compounds
widely
diffused
in
plants,
especially
Chinese
herbs,
are
used
as
functional
ingredients,
precursors,
lead
food
clinical
applications.
Among
them,
aporphine
alkaloids
(AAs),
an
important
isoquinoline
alkaloids,
exert
strong
anticancer
effect
on
multiple
cancer
types.
This
review
aims
to
comprehensively
summarize
phytochemistry,
pharmacokinetics,
bioavailability
seven
subtypes
AAs
their
derivatives
from
various
plants
highlight
bioactivities
mechanisms
action.
Key
Scientific
Concepts
Review.
The
chemical
structures
botanical
diversity
elucidated,
promising
results
highlighted
regarding
potent
activities
derivatives,
contributing
pharmacological
benefits.
work
provides
better
understanding
combinational
therapies
involving
thereby
improving
development
containing
plant-derived
AA
application
AAs.
Язык: Английский
Magnoflorine alleviates dextran sulfate sodium‐induced ulcerative colitis via inhibiting JAK2/STAT3 signaling pathway
Phytotherapy Research,
Год журнала:
2024,
Номер
38(9), С. 4592 - 4613
Опубликована: Июль 30, 2024
Abstract
Magnoflorine
(Mag),
a
natural
alkaloid
component
originating
from
the
Ranunculaceae
Juss
.
Family,
has
various
of
pharmacological
activities.
This
study
aimed
to
investigate
therapeutic
effects
and
potential
mechanism
Mag
on
dextran
sulfate
sodium
(DSS)‐induced
ulcerative
colitis
(UC)
based
comprehensive
approaches.
Therapeutic
3%
DSS‐induced
UC
mice
were
analyzed.
UHPLC‐Q‐TOF/MS
was
performed
metabolites
signaling
pathway
UC.
Furthermore,
predicted
mRNA
protein
levels
JAK2/STAT3
in
colon
tissue
verified
assessed
by
qRT‐PCR
Western
Blotting,
respectively.
presented
down‐regulation
serum
biochemical
indices,
alleviating
histological
damage
tissue.
Serum
untargeted
metabolomics
analysis
showed
that
is
mainly
associated
with
regulation
six
biomarkers
11
pathways,
which
may
be
responsible
for
efficacy
The
“component–metabolites–targets”
interactive
network
indicated
exerts
its
anti‐UC
effect
regulating
PTGS1
PTGS2,
thereby
arachidonic
acid.
Moreover,
results
could
substantially
decrease
relative
expression
level
Hub
genes.
In
addition,
it
found
inhibit
pathway.
present
highlighted
role
ameliorated
injury
inhibiting
These
suggest
an
effective
agent
treatment
Язык: Английский
A Combination of Magnoflorine and Spinosin Improves the Antidepressant effects on CUMS Mouse Model
Current Drug Metabolism,
Год журнала:
2024,
Номер
25(1), С. 71 - 80
Опубликована: Янв. 1, 2024
Background:
Depression
is
a
common
neuropsychiatric
disease.
As
famous
traditional
Chinese
medicine
with
significant
anti-depressive
and
sleep-promoting
effects,
Ziziphi
Spinosae
Semen
(ZSS)
has
attracted
the
attention
of
many
researchers.
Although
it
well
known
that
Magnoflorine
(MAG)
Spinosin
(SPI)
were
main
active
components
isolated
from
ZSS,
there
lack
research
on
combined
treatment
depression
these
two
ingredients.
Methods:
The
shaking
bottle
method
was
used
to
simulate
human
environment
for
detecting
changes
in
oil-water
partition
coefficient
before
after
drug
combination.
Cell
viability
evaluated
by
MTT
assay.
To
establish
mouse
model
insomnia
CUMS
method,
then
explore
effect
administration
MAG
SPI
observing
behavior
analyzing
pharmacokinetics.
Results:
change
LogP
values
affected
lipid
solubility
increased
water
SPI,
allowing
them
penetrate
more
easily
through
blood-brain
barrier
into
brain.
Compared
group,
MAG-SPI
concentration
60
μM
significantly
cell
survival
rate.
In
both
TST
FST
experiments,
mice
showed
decrease
immobilization
time.
Pharmacokinetic
results
pharmacokinetic
parameters,
Cmax
AUC
case
combination,
which
resulted
enhancement
their
relative
bioavailability
improvement
vivo
effects.
Conclusions:
present
study
demonstrated
combination
had
synergistic
antidepressant
model.
Язык: Английский
Optimized Centrifugal Partition Chromatography (CPC) Protocol for Isolation of Urease Inhibitors: Magnoflorine and Berberine from Berberis vulgaris Extracts
Separations,
Год журнала:
2024,
Номер
11(4), С. 94 - 94
Опубликована: Март 25, 2024
In
recent
years,
an
increasing
interest
in
phytotherapy
has
been
observed.
Parallel
to
the
research
on
total
extracts
of
plant
material,
numerous
studies
activity
single
molecules
derived
from
plants
are
being
conducted
address
their
mechanisms
action
and
determine
active
doses
eventual
interactions.
Despite
this
phenomenon,
isolation
individual
compounds
is
a
bottleneck
due
its
difficulty
cost.
This
work
presents
results
careful
optimization
magnoflorine
berberine
(isoquinoline
alkaloids)
recovery
commonly
distributed
shrub,
Berberis
vulgaris,
growing
Poland
Georgia,
using
CPC.
Both
known
for
medicinal
properties,
which
makes
methodology
important
area
research.
Additionally,
CPC
ability
isolate
high-quality
large
quantities,
it
effective
easy-to-commercialize
method.
For
successful
separation,
biphasic
solvent
system
composed
hexane,
butanol,
ethanol,
water
ratio
(3:12:4:16
v/v/v/v)
was
used
ascending
mode,
together
with
flow
rate
8
mL/min
rotation
speed
1600
rpm.
The
method
selective
both
compounds,
delivered
good
root
stem
plant.
qualitative
composition
alkaloids
studied
determined
by
HPLC-ESI-QTOF-MS/MS
confirmed
presence
berberine,
magnoflorine,
jatrorhizine,
palmatine
group
isoquinolines.
isolates,
were
subjected
Helicobacter
pylori
growth
inhibition
assay
urease
test
assess
whether,
next
previously
proved
anticancer
these
characterized
H.
inhibition.
MGN
found
exhibit
inhibitory
potential
against
(IC50
=
25
mg/L).
Язык: Английский
Structural characterization and biological activity evaluation of Magnoflorine alkaloid, a potential anticonvulsant agent.
Journal of Molecular Structure,
Год журнала:
2024,
Номер
1317, С. 139036 - 139036
Опубликована: Июнь 14, 2024
Язык: Английский
Heterogeneous Cellular Response of Primary and Metastatic Human Gastric Adenocarcinoma Cell Lines to Magnoflorine and Its Additive Interaction with Docetaxel
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(21), С. 15511 - 15511
Опубликована: Окт. 24, 2023
Gastric
cancer
is
the
most
common
and
remains
leading
cause
of
death
worldwide.
In
this
study,
anticancer
action
magnoflorine
isolated
via
counter-current
chromatography
from
methanolic
extract
Berberis
vulgaris
root
against
gastric
in
models
primary
ACC-201
AGS
metastatic
MKN-74
NCI-N87
cell
lines
was
analyzed.
Cell
viability
proliferation
were
tested
through
use
MTT
BrdU
tests,
respectively.
cycle
progression
apoptosis
evaluated
using
flow
cytometry.
The
interaction
docetaxel
has
been
examined
isobolographic
analysis.
Moreover,
potential
toxicity
verified
zebrafish
an
vivo
model.
revealed
different
responses
to
treatment
with
regard
viability/proliferation,
induction
inhibition
without
any
undesirable
changes
development
larval
at
concentrations.
What
more,
combination
produced
additive
pharmacological
all
studied
lines,
which
may
suggest
a
complementary
mechanism
both
compounds.
Taken
together,
these
findings
provide
foundation
for
possibility
as
therapeutic
approach
merits
further
investigation,
pave
way
clinical
uses
magnoflorine.
Язык: Английский
A comprehensive assessment of palmatine as anticonvulsant agent – In vivo and in silico studies
Biomedicine & Pharmacotherapy,
Год журнала:
2024,
Номер
172, С. 116234 - 116234
Опубликована: Фев. 6, 2024
Previously,
we
demonstrated
that
palmatine
(PALM)
-
an
isoquinoline
alkaloid
from
Berberis
sibrica
radix,
exerted
antiseizure
activity
in
the
pentylenetetrazole
(PTZ)-induced
seizure
assay
larval
zebrafish.
The
aim
of
present
study
was
to
more
precisely
characterize
PALM
as
a
potential
anticonvulsant
drug
candidate.
A
range
zebrafish
and
mouse
seizure/epilepsy
models
were
applied
investigation.
Immunostaining
analysis
conducted
assess
changes
brains,
while
silico
molecular
modelling
performed
determine
targets
for
PALM.
Accordingly,
had
effect
ethyl
2-ketopent-4-enoate
(EKP)-induced
larvae
well
6
Hz-induced
psychomotor
threshold
timed
infusion
PTZ
tests
mice.
protective
EKP-induced
confirmed
local
field
recordings.
did
not
affect
seizures
gabra1a
knockout
line
larvae.
In
scn1Lab
Язык: Английский
Can Magnoflorine Improve Memory? Immunohistochemical Studies on Parvalbumin Immunoreactive Neurons and Fibers of Mice Hippocampus
Nutrients,
Год журнала:
2024,
Номер
17(1), С. 137 - 137
Опубликована: Дек. 31, 2024
Background/Objectives:
We
assessed
the
influence
of
long-term
injection
magnoflorine
(MAG)
on
memory
acquisition
in
mice
for
first
time.
Methods:
This
isoquinoline
alkaloid
that
belongs
to
aporphines
was
isolated
from
roots
Berberis
vulgaris
by
centrifugal
partition
chromatography
(CPC)
using
a
biphasic
solvent
system
composed
chloroform:
methanol:
water
ratio
4:3:3
(v/v/v)
with
20
mM
hydrochloric
acid
and
triethylamine,
within
64
min.
Results:
Our
results
indicated
MAG
mg/kg
dose
improve
were
evaluated
passive
avoidance
(PA)
test
no
toxicity
records.
The
analysis
brain
lysates
animal
plasma
HPLC-ESI-QTOF-MS/MS
showed
ability
compound
cross
blood–brain
barrier,
an
elevated
level
phosphatidylcholine
PC
(14:1(9Z)/14:1(9Z))
molecular
formula
C36H69NO8P
observed
both
treated
groups
10
comparison
control
group.
Conclusions:
phenomenon
may
explain
MAG’s
cognition-enhancing
properties
as
induce
synthesis
strengthening
neuronal
cells.
Also,
7-day-long
administration
at
increased
mean
number
parvalbumin
(PV)-IR
neurons
hippocampus.
Statistically,
largest
PV-IR
dose,
which
indicate
potential
effect
Ca2+
metabolism.
However,
statistical
differences
nerve
fibers
doses
MAG,
regardless
hippocampal
fields.
positive
provides
further
support
neuroprotective
this
alkaloid.
Язык: Английский