Current topics in membranes, Год журнала: 2024, Номер unknown, С. 85 - 106
Опубликована: Янв. 1, 2024
Язык: Английский
Cells, Год журнала: 2025, Номер 14(2), С. 115 - 115
Опубликована: Янв. 14, 2025
tRNA molecules are among the most fundamental and evolutionarily conserved RNA types, primarily facilitating translation of genetic information from mRNA into proteins. Beyond their canonical role as adaptor during protein synthesis, tRNAs have evolved to perform additional functions. One such non-canonical for is through generation tRNA-derived fragments via specific cleavage processes. These small RNAs (tsRNAs) present across all three domains life, including in protozoan parasites. They formed parent at different sites, resulting either halves or smaller fragments. The precise mechanisms underlying synthesis various fragments, RNases involved, well distinct functions roles parasite physiology, not yet fully understood remain an active area ongoing research. However, modulating gene expression, particularly stress responses, becoming increasingly evident. In this context, we discuss recent findings on Furthermore, investigate how these tsRNAs modulate expression within itself packaged extracellular vesicles alter host thereby promoting survival adaptation.
Язык: Английский
Процитировано
1
Proceedings of the National Academy of Sciences, Год журнала: 2024, Номер 121(25)
Опубликована: Июнь 12, 2024
Trichomonas vaginalis , a common sexually transmitted parasite that colonizes the human urogenital tract, secretes extracellular vesicles (TvEVs) are taken up by cells and speculated to be parasites as well. While crosstalk between TvEVs has led insight into host:parasite interactions, roles for in infection have largely been one-sided, with little known about effect of TvEV uptake T. . Approximately 11% infections found coinfections multiple strains. Clinical isolates often differ their adherence cytolysis host cells, underscoring importance understanding effects within population. To address this question, our lab tested ability less adherent strain G3, take fluorescently labeled derived from both itself (G3-EVs) more (B7RC2-EVs). Here, we showed generated internalized efficiently compared strain. Additionally, preincubation G3 B7RC2-EVs increases aggregation cells. Transcriptomics revealed up-regulate expression predicted membrane proteins identified an factor, heteropolysaccharide binding protein (HPB2). Finally, using comparative proteomics superresolution microscopy, demonstrated direct transfer cadherin-like protein, recipient parasite’s surface. This work identifies mediator parasite:parasite communication may impact pathogenesis during mixed infections.
Язык: Английский
Процитировано
5
Tropical Medicine and Infectious Disease, Год журнала: 2023, Номер 8(9), С. 448 - 448
Опубликована: Сен. 15, 2023
Extracellular vesicles (EVs) are lipid-membrane-bound structures produced naturally by all cells and have a variety of functions. EVs act as vehicles for transporting important molecular signals from one cell to another. Several parasites been shown secrete EVs, their biological functions extensively studied. facilitate communication with the host (such modulation host’s immune system or promoting attachment invasion into cells) between parasitic (e.g., transferring drug-resistance genes factors modulating stage conversion). It is clear that play an role in host–parasite interactions. In this review, we summarized latest research on secreted protozoan parasite–parasite communications.
Язык: Английский
Процитировано
12
Microbes and Infection, Год журнала: 2024, Номер 26(4), С. 105310 - 105310
Опубликована: Фев. 4, 2024
Язык: Английский
Процитировано
4
Parasites & Vectors, Год журнала: 2025, Номер 18(1)
Опубликована: Фев. 24, 2025
Abstract Background Giardia duodenalis is a prevalent intestinal pathogen causing giardiasis, condition characterized by diarrhea and frequently linked to malnutrition growth impairments in children. The virulence of Giardiavirus (GLV) may efficiently clear parasites from infected patients. However, we have limited understanding GLV transmission among species GLV-infected ’s impact on pathogenicity. Methods This study investigated extracellular vesicles (EVs) isolated via ultracentrifugation or exosome assay kit detect the presence EVs, results were detected using ultrastructure molecular methods, including electron microscopy, scanning quantitative polymerase chain reaction (qPCR), dot blot. Transwell migration assays confirmed spread GLV-enveloped EVs inhibitor experiments immunofluorescence. Mice gavaged with , without infection, assessed for disease progression, parameters (weight size gains), permeability, pathology. Results Parts exploit pathway reach environment, allowing these EVs. uptake GLV-containing rapid trophozoite wrapped also offers protection against external interference. Importantly, EV-coated leads divergent clinical symptoms mice, posing less risk mice reducing symptoms, such as emaciation, stunted growth, lesion damage, compared GLV-free -infected mice. Conclusions Our studies show that can species, infection alleviates lesions caused . These findings reveal could be target development novel intervention strategies Graphical abstract
Язык: Английский
Процитировано
0
Animals and zoonoses., Год журнала: 2025, Номер unknown
Опубликована: Март 1, 2025
Язык: Английский
Процитировано
0
Veterinary Sciences, Год журнала: 2024, Номер 11(7), С. 321 - 321
Опубликована: Июль 17, 2024
Three genera of viruses the family
Язык: Английский
Процитировано
3
Current topics in membranes, Год журнала: 2024, Номер unknown, С. 187 - 223
Опубликована: Янв. 1, 2024
Язык: Английский
Процитировано
3
Journal of Extracellular Biology, Год журнала: 2023, Номер 2(9)
Опубликована: Авг. 30, 2023
Abstract Extracellular vesicles (EVs) recently emerged as important players in the pathophysiology of parasitic infections. While protist parasite Giardia duodenalis can produce EVs, their role giardiasis remains obscure. disrupt gut microbiota biofilms and transform commensal bacteria into invasive pathobionts at sites devoid colonizing trophozoites via unknown mechanisms. We hypothesized that EVs could modify bacterial behaviour a novel mode trans‐kingdom communication. Our findings indicate exert bacteriostatic effects on Escherichia coli HB101 Enterobacter cloacae TW1, increasing swimming motility. also decreased biofilm‐forming ability E. but not by supporting hypothesis these are, least part, bacteria‐selective. TW1 exhibited increased adhesion/invasion onto small intestine epithelial cells when exposed to EVs. labelled with PKH67 revealed colocalization cells. Small RNA sequencing high abundance ribosomal (rRNA)‐ transfer (tRNA)‐derived RNAs, short‐interfering RNAs (siRNAs) micro‐RNAs (miRNAs) within Proteomic analysis uncovered presence chaperones heat shock proteins facilitate thermal stability its sRNA cargo, well protein‐modifying enzymes. In vitro, RNase heat‐treatment assays showed total proteins, are responsible for modulating motility biofilm formation. G. were adhesion intestinal induced upon exposure Together, contain heat‐stable, RNase‐sensitive cargo trigger development pathobiont characteristics Enterobacteria, depicting cross‐talk gut.
Язык: Английский
Процитировано
8
Experimental Parasitology, Год журнала: 2024, Номер 262, С. 108788 - 108788
Опубликована: Май 15, 2024
Язык: Английский
Процитировано
2