Biology,
Год журнала:
2024,
Номер
13(7), С. 463 - 463
Опубликована: Июнь 21, 2024
Breast
cancer
is
the
most
prevalent
among
women
worldwide.
Therapeutic
strategies
to
control
tumors
and
metastasis
are
still
challenging.
Three-dimensional
(3D)
spheroid-type
systems
more
accurately
replicate
features
of
in
vivo,
working
as
a
better
platform
for
performing
therapeutic
response
analysis.
This
work
aimed
characterize
epithelial-mesenchymal
transition
doxorubicin
(dox)
mammary
tumor
spheroid
(MTS)
model.
We
evaluated
treatment
effect
on
MCF-7
diameter,
cell
viability,
death,
migration
proteins
involved
(EMT)
process.
Spheroids
were
also
produced
from
formed
4T1
67NR
lines.
MTSs
mimicked
avascular
characteristics,
exhibited
adherens
junction
independently
their
own
extracellular
matrix.
Our
model
supports
3D
culturing
cells
isolated
mice
tumors.
Through
assay,
we
verified
reduction
E-cadherin
expression
an
increase
vimentin
became
distant
spheroids.
Dox
promoted
cytotoxicity
inhibited
EMT
These
results
suggest,
first
time,
that
this
reproduces
aspects
process
describes
potential
dox
inhibiting
metastatic
process,
which
can
be
further
explored.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(5), С. 2512 - 2512
Опубликована: Фев. 21, 2024
Bone
differentiation
is
crucial
for
skeletal
development
and
maintenance.
Its
dysfunction
can
cause
various
pathological
conditions
such
as
rickets,
osteoporosis,
osteogenesis
imperfecta,
or
Paget's
disease.
Although
traditional
two-dimensional
cell
culture
systems
have
contributed
significantly
to
our
understanding
of
bone
biology,
they
fail
replicate
the
intricate
biotic
environment
tissue.
Three-dimensional
(3D)
spheroid
cultures
gained
widespread
popularity
addressing
defects.
This
review
highlights
advantages
employing
3D
investigate
differentiation.
It
their
capacity
mimic
complex
in
vivo
cellular
interactions
pivotal
homeostasis.
The
exploration
models
research
offers
enhanced
physiological
relevance,
improved
predictive
capabilities,
reduced
reliance
on
animal
models,
which
advancement
safer
more
effective
strategies
drug
development.
Studies
highlighted
transformative
potential
expanding
biology
developing
targeted
therapeutic
interventions
bone-related
disorders.
explores
how
demonstrated
promise
unraveling
mechanisms
governing
homeostasis
responses
pharmacological
agents.
Abstract
Cancer
neuroscience
is
an
emerging
field
that
investigates
the
intricate
relationship
between
nervous
system
and
cancer,
gaining
increasing
recognition
for
its
importance.
The
central
governs
development
of
directly
affects
brain
tumors,
peripheral
(PNS)
shapes
tumor
microenvironment
(TME)
tumors.
Both
systems
are
crucial
in
cancer
initiation
progression,
with
recent
studies
revealing
a
more
role
PNS
within
TME.
Tumors
not
only
invade
nerves
but
also
persuade
them
through
remodeling
to
further
promote
malignancy,
creating
bidirectional
interaction
cancers.
Notably,
immune
cells
contribute
this
communication,
forming
triangular
influences
protumor
inflammation
effectiveness
immunotherapy.
This
review
delves
into
mechanisms
connecting
focusing
on
how
various
cell
types
influence
nerve‒tumor
interactions,
emphasizing
clinical
relevance
nerve‒immune
dynamics.
By
deepening
our
understanding
interplay
nerves,
cells,
has
potential
reshape
biology
insights,
inspire
innovative
therapies,
improve
outcomes
patients.
Biosensors,
Год журнала:
2024,
Номер
14(6), С. 301 - 301
Опубликована: Июнь 8, 2024
Three-dimensional
(3D)
printing
presents
a
compelling
alternative
for
fabricating
microfluidic
devices,
circumventing
certain
limitations
associated
with
traditional
soft
lithography
methods.
Microfluidics
play
crucial
role
in
the
biomedical
sciences,
particularly
creation
of
tissue
spheroids
and
pharmaceutical
research.
Among
various
3D
techniques,
light-driven
methods
such
as
stereolithography
(SLA),
digital
light
processing
(DLP),
photopolymer
inkjet
have
gained
prominence
microfluidics
due
to
their
rapid
prototyping
capabilities,
high-resolution
printing,
low
temperatures.
This
review
offers
comprehensive
overview
techniques
used
fabrication
advanced
devices.
It
explores
applications
3D-printed
provides
insights
into
potential
impact
functionality
within
field.
We
further
summarize
three
strategies
producing
systems:
direct
construction
devices
cell
culture,
PDMS-based
engineering,
modular
SLA-printed
chip
co-culture
monitor
cells.
Wound Repair and Regeneration,
Год журнала:
2025,
Номер
33(1)
Опубликована: Янв. 1, 2025
Abstract
As
the
number
of
patients
requiring
organ
transplants
continues
to
rise
exponentially,
there
is
a
dire
need
for
therapeutics,
with
repair
and
regenerative
properties,
assist
in
alleviating
this
medical
crisis.
Over
past
decade,
has
been
shift
from
conventional
stem
cell
treatments
towards
use
secretome,
protein
factor
secretions
cells.
These
components
may
possess
novel
druggable
targets
hold
key
profoundly
altering
field
medicine.
Despite
progress
field,
clinical
translation
secretome‐containing
products
limited
by
several
challenges
including
but
not
ensuring
batch‐to‐batch
consistency,
prevention
further
heterogeneity,
production
sufficient
secretome
quantities,
product
registration,
good
manufacturing
practice
protocols
pharmacokinetic/pharmacodynamic
profiles
all
components.
this,
unlocking
blockage
scientists
have
encountered
years.
This
review
critically
analyses
derived
different
sources
used
tissues
tissue
regeneration.
Furthermore,
it
provides
an
overview
current
delivery
strategies
future
perspectives
as
potential
therapeutic.
The
success
possible
shortcomings
are
evaluated.
Pharmaceuticals,
Год журнала:
2025,
Номер
18(2), С. 195 - 195
Опубликована: Янв. 31, 2025
Drugs
administered
by
means
of
extravascular
routes
drug
administration
must
be
absorbed
into
the
systemic
circulation,
which
involves
movement
molecules
across
biological
barriers
such
as
epithelial
cells
that
cover
mucosal
surfaces
or
stratum
corneum
covers
skin.
Some
drugs
exhibit
poor
permeation
membranes
may
experience
excessive
degradation
during
first-pass
metabolism,
tends
to
limit
their
bioavailability.
Various
strategies
have
been
used
improve
Absorption
enhancement
include
co-administration
chemical
enhancers,
enzymes,
and/or
efflux
transporter
inhibitors,
changes,
and
specialized
dosage
form
designs.
Models
with
physiological
relevance
are
needed
evaluate
efficacy
absorption
techniques.
in
vitro
cell
culture
models
ex
vivo
tissue
explored
quantify
effectiveness
strategies.
This
review
deliberates
on
use
for
evaluation
selected
including
nasal,
oromucosal,
pulmonary,
oral,
rectal,
transdermal
administration.
Journal of Tissue Engineering,
Год журнала:
2025,
Номер
16
Опубликована: Янв. 1, 2025
The
benefit
of
complex
3D
models
to
facilitate
the
robust
testing
new
drugs
and
drug
delivery
systems
during
developmental
stages
pharmaceutical
manufacturing
has
recently
become
distinguished
within
field.
Recognition
this
need
by
industry
provided
a
motivation
for
research
into
development
reliable
use
in
delivery,
biomaterials,
tissue
engineering.
Both
vitro
ex
vivo
can
enhance
drug-testing
discovery
prospects
over
more
traditionally
used
2D,
monolayer
culture
animal
models.
Despite
widespread
acceptance
that
modelling
is
advantageous
field,
there
remains
lack
standardisation
throughout
literature.
This
article
provides
an
extensive
review
current
literature
on
vitro,
oral
mucosa
applications;
advantages,
limitations,
recommendations
future
improved
application.
In
vitro
cytotoxicity
assays
are
critical
tools
for
assessing
the
potential
toxicity
of
compounds
in
early-stage
drug
discovery,
toxicology,
and
biomedical
research.
These
help
evaluate
effects
chemical,
pharmaceutical,
biological
agents
on
cellular
health
viability.
However,
reliability
data
is
often
compromised
by
a
variety
experimental
pitfalls.
This
chapter
discusses
key
factors
that
can
lead
to
inaccurate
or
misleading
results
provides
guidelines
avoiding
them.
Common
issues
such
as
inappropriate
assay
selection,
interference
from
serum
components
like
fetal
bovine
(FBS),
cell
density
variations,
incorrect
incubation
times
highlighted.
The
importance
using
appropriate
controls,
solvent-induced
toxicity,
preventing
compound
aggregation
also
emphasized.
By
optimizing
conditions
understanding
limitations
each
type,
researchers
enhance
precision
their
tests.
aims
provide
comprehensive
guide
improving
design,
ensuring
reproducibility,
generating
reliable
be
confidently
applied
development
toxicological
evaluations.
