Spheroid Model of Mammary Tumor Cells: Epithelial–Mesenchymal Transition and Doxorubicin Response DOI Creative Commons
Laura Lacerda Coelho, Matheus Menezes Vianna, Debora Moraes da Silva

et al.

Biology, Journal Year: 2024, Volume and Issue: 13(7), P. 463 - 463

Published: June 21, 2024

Breast cancer is the most prevalent among women worldwide. Therapeutic strategies to control tumors and metastasis are still challenging. Three-dimensional (3D) spheroid-type systems more accurately replicate features of in vivo, working as a better platform for performing therapeutic response analysis. This work aimed characterize epithelial-mesenchymal transition doxorubicin (dox) mammary tumor spheroid (MTS) model. We evaluated treatment effect on MCF-7 diameter, cell viability, death, migration proteins involved (EMT) process. Spheroids were also produced from formed 4T1 67NR lines. MTSs mimicked avascular characteristics, exhibited adherens junction independently their own extracellular matrix. Our model supports 3D culturing cells isolated mice tumors. Through assay, we verified reduction E-cadherin expression an increase vimentin became distant spheroids. Dox promoted cytotoxicity inhibited EMT These results suggest, first time, that this reproduces aspects process describes potential dox inhibiting metastatic process, which can be further explored.

Language: Английский

Alternatives of Animal Models for Biomedical Research: a Comprehensive Review of Modern Approaches DOI
Abhinav Vashishat, Preeti Patel, Ghanshyam Das Gupta

et al.

Stem Cell Reviews and Reports, Journal Year: 2024, Volume and Issue: 20(4), P. 881 - 899

Published: March 1, 2024

Language: Английский

Citations

22
Exploring the Anti-Colon Cancer Potential of Polygonum minus: Integrating In Vitro and In Silico Studies DOI
Zhongming Yang, Zhongguo Yang,

Md Zuki Abu Bakar

et al.

Food Bioscience, Journal Year: 2025, Volume and Issue: 64, P. 105853 - 105853

Published: Jan. 6, 2025

Language: Английский

Citations

3
Advantages of Using 3D Spheroid Culture Systems in Toxicological and Pharmacological Assessment for Osteogenesis Research DOI Open Access
Chawon Yun, Sou Hyun Kim,

Kyung Mok Kim

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(5), P. 2512 - 2512

Published: Feb. 21, 2024

Bone differentiation is crucial for skeletal development and maintenance. Its dysfunction can cause various pathological conditions such as rickets, osteoporosis, osteogenesis imperfecta, or Paget's disease. Although traditional two-dimensional cell culture systems have contributed significantly to our understanding of bone biology, they fail replicate the intricate biotic environment tissue. Three-dimensional (3D) spheroid cultures gained widespread popularity addressing defects. This review highlights advantages employing 3D investigate differentiation. It their capacity mimic complex in vivo cellular interactions pivotal homeostasis. The exploration models research offers enhanced physiological relevance, improved predictive capabilities, reduced reliance on animal models, which advancement safer more effective strategies drug development. Studies highlighted transformative potential expanding biology developing targeted therapeutic interventions bone-related disorders. explores how demonstrated promise unraveling mechanisms governing homeostasis responses pharmacological agents.

Language: Английский

Citations

7
Neuroscience in peripheral cancers: tumors hijacking nerves and neuroimmune crosstalk DOI Creative Commons

Hua‐yang Fan,

Xin‐hua Liang, Ya‐ling Tang

et al.

MedComm, Journal Year: 2024, Volume and Issue: 5(11)

Published: Oct. 31, 2024

Abstract Cancer neuroscience is an emerging field that investigates the intricate relationship between nervous system and cancer, gaining increasing recognition for its importance. The central governs development of directly affects brain tumors, peripheral (PNS) shapes tumor microenvironment (TME) tumors. Both systems are crucial in cancer initiation progression, with recent studies revealing a more role PNS within TME. Tumors not only invade nerves but also persuade them through remodeling to further promote malignancy, creating bidirectional interaction cancers. Notably, immune cells contribute this communication, forming triangular influences protumor inflammation effectiveness immunotherapy. This review delves into mechanisms connecting focusing on how various cell types influence nerve‒tumor interactions, emphasizing clinical relevance nerve‒immune dynamics. By deepening our understanding interplay nerves, cells, has potential reshape biology insights, inspire innovative therapies, improve outcomes patients.

Language: Английский

Citations

6
Advancing Tissue Culture with Light-Driven 3D-Printed Microfluidic Devices DOI Creative Commons
Xiangke Li, Meng Wang, Thomas P. Davis

et al.

Biosensors, Journal Year: 2024, Volume and Issue: 14(6), P. 301 - 301

Published: June 8, 2024

Three-dimensional (3D) printing presents a compelling alternative for fabricating microfluidic devices, circumventing certain limitations associated with traditional soft lithography methods. Microfluidics play crucial role in the biomedical sciences, particularly creation of tissue spheroids and pharmaceutical research. Among various 3D techniques, light-driven methods such as stereolithography (SLA), digital light processing (DLP), photopolymer inkjet have gained prominence microfluidics due to their rapid prototyping capabilities, high-resolution printing, low temperatures. This review offers comprehensive overview techniques used fabrication advanced devices. It explores applications 3D-printed provides insights into potential impact functionality within field. We further summarize three strategies producing systems: direct construction devices cell culture, PDMS-based engineering, modular SLA-printed chip co-culture monitor cells.

Language: Английский

Citations

4
Electrospinning of microspheres with ectodermal mesenchymal stem cells for vascular regeneration DOI

Jiangnan Yu,

Min Ni,

Kai Liu

et al.

European Polymer Journal, Journal Year: 2025, Volume and Issue: unknown, P. 113716 - 113716

Published: Jan. 1, 2025

Language: Английский

Citations

0
The paradigm of stem cell secretome in tissue repair and regeneration: Present and future perspectives DOI Creative Commons
Kate Da Silva, Pradeep Kumar, Yahya E. Choonara

et al.

Wound Repair and Regeneration, Journal Year: 2025, Volume and Issue: 33(1)

Published: Jan. 1, 2025

Abstract As the number of patients requiring organ transplants continues to rise exponentially, there is a dire need for therapeutics, with repair and regenerative properties, assist in alleviating this medical crisis. Over past decade, has been shift from conventional stem cell treatments towards use secretome, protein factor secretions cells. These components may possess novel druggable targets hold key profoundly altering field medicine. Despite progress field, clinical translation secretome‐containing products limited by several challenges including but not ensuring batch‐to‐batch consistency, prevention further heterogeneity, production sufficient secretome quantities, product registration, good manufacturing practice protocols pharmacokinetic/pharmacodynamic profiles all components. this, unlocking blockage scientists have encountered years. This review critically analyses derived different sources used tissues tissue regeneration. Furthermore, it provides an overview current delivery strategies future perspectives as potential therapeutic. The success possible shortcomings are evaluated.

Language: Английский

Citations

0
Evaluation of Drug Permeation Enhancement by Using In Vitro and Ex Vivo Models DOI Creative Commons
Dewald Steyn, Anja Haasbroek‐Pheiffer, Wihan Pheiffer

et al.

Pharmaceuticals, Journal Year: 2025, Volume and Issue: 18(2), P. 195 - 195

Published: Jan. 31, 2025

Drugs administered by means of extravascular routes drug administration must be absorbed into the systemic circulation, which involves movement molecules across biological barriers such as epithelial cells that cover mucosal surfaces or stratum corneum covers skin. Some drugs exhibit poor permeation membranes may experience excessive degradation during first-pass metabolism, tends to limit their bioavailability. Various strategies have been used improve Absorption enhancement include co-administration chemical enhancers, enzymes, and/or efflux transporter inhibitors, changes, and specialized dosage form designs. Models with physiological relevance are needed evaluate efficacy absorption techniques. in vitro cell culture models ex vivo tissue explored quantify effectiveness strategies. This review deliberates on use for evaluation selected including nasal, oromucosal, pulmonary, oral, rectal, transdermal administration.

Language: Английский

Citations

0
In vitro and ex vivo models of the oral mucosa as platforms for the validation of novel drug delivery systems DOI Creative Commons
Robyn A. Macartney,

A. K. Das,

Atina G Imaniyyah

et al.

Journal of Tissue Engineering, Journal Year: 2025, Volume and Issue: 16

Published: Jan. 1, 2025

The benefit of complex 3D models to facilitate the robust testing new drugs and drug delivery systems during developmental stages pharmaceutical manufacturing has recently become distinguished within field. Recognition this need by industry provided a motivation for research into development reliable use in delivery, biomaterials, tissue engineering. Both vitro ex vivo can enhance drug-testing discovery prospects over more traditionally used 2D, monolayer culture animal models. Despite widespread acceptance that modelling is advantageous field, there remains lack standardisation throughout literature. This article provides an extensive review current literature on vitro, oral mucosa applications; advantages, limitations, recommendations future improved application.

Language: Английский

Citations

0
In Vitro Cytotoxicity Determination: Avoiding Pitfalls DOI Creative Commons

Mashilo Mash Matotoka,

Peter Masoko

Biochemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 19, 2025

In vitro cytotoxicity assays are critical tools for assessing the potential toxicity of compounds in early-stage drug discovery, toxicology, and biomedical research. These help evaluate effects chemical, pharmaceutical, biological agents on cellular health viability. However, reliability data is often compromised by a variety experimental pitfalls. This chapter discusses key factors that can lead to inaccurate or misleading results provides guidelines avoiding them. Common issues such as inappropriate assay selection, interference from serum components like fetal bovine (FBS), cell density variations, incorrect incubation times highlighted. The importance using appropriate controls, solvent-induced toxicity, preventing compound aggregation also emphasized. By optimizing conditions understanding limitations each type, researchers enhance precision their tests. aims provide comprehensive guide improving design, ensuring reproducibility, generating reliable be confidently applied development toxicological evaluations.

Language: Английский

Citations

0