Integrating Microfluidics, Hydrogels, and 3D Bioprinting for Personalized Vessel-on-a-Chip Platforms

Biomaterials Science, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Advancement of vascular models from simple 2D culture to complex vessel-on-a-chip platforms through integration microfluidics, biomimetic hydrogels, and 3D bioprinting, enabling controlled investigation thrombosis mechanisms.

Язык: Английский

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Characterization of Sublingual Microvascular Tortuosity in Steady-State Physiology and Septic Shock

Biomedicines, Год журнала: 2025, Номер 13(3), С. 691 - 691

Опубликована: Март 11, 2025

Background: The characteristics of hemodynamic coherence in healthy states and disease remain unknown. Capillary tortuosity is a morphologic variant microcirculatory vessels, but its effects have generally not been considered the assessment tissue perfusion oxygenation. We investigated role sublingual capillary anesthetized adult individuals with steady-state physiology (ASA 1) patients septic shock requiring emergency abdominal surgery 4E 5E). Methods: Sublingual macro variables, oxygen transport, metabolic parameters, score (CTS) were assessed. Results: Mean (SD) CTS was 0.55 (0.76) 3.31 (0.86) group, respectively (p < 0.001). In shock, significantly associated alveolar-to-arterial gradient (r = 0.658, p 0.015) debt −0.769, 0.002). Significant differences also observed Consensus Proportion Perfused Vessels (PPV; 0.001), PPV (small) Microvascular Flow Index vessel diameter 0.001) length wall shear stress lactate extraction ratio arterial content venous delivery consumption 0.002) between two groups. Conclusions: essentially absent physiology. contrast, it increased Alveolar-to-arterial critically ill shock.

Язык: Английский

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Effect of Centhaquine on the Coagulation Cascade in Normal State and Uncontrolled Hemorrhage: A Multiphase Study Combining Ex Vivo and In Vivo Experiments in Different Species

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(6), С. 3494 - 3494

Опубликована: Март 20, 2024

Centhaquine is a novel vasopressor acting on α2A- and α2B-adrenoreceptors, increasing venous return improving tissue perfusion. We investigated the effects of centhaquine blood coagulation in normal state uncontrolled hemorrhage using ex vivo experiments different species. Thromboelastography (TEG) parameters included clotting time (R), clot kinetics [K angle (α)], strength (MA), percent lysis 30 min post-MA (LY30). In rat blood, did not alter R, K, α, MA, or LY30 values vehicle group antithrombotic aspirin heparin. Subsequently, New Zealand white rabbits with were assigned to three resuscitation groups: Sal-MAP 45 (normal saline maintain mean arterial pressure, MAP, mmHg), Centh-MAP (0.05 mg kg−1 plus MAP 60 mmHg). The was characterized by no change reduced K increased α. group, TEG showed α compared saline; however, MA significantly (p = 0.018). an increase < 0.001), decrease 0.01), 0.029) group. conclusion, does impair facilitates hemostatic resuscitation.

Язык: Английский

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Intestinal oxygen utilisation and cellular adaptation during intestinal ischaemia–reperfusion injury

Clinical and Translational Medicine, Год журнала: 2024, Номер 15(1)

Опубликована: Дек. 26, 2024

Язык: Английский

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Protease-Activated Receptors (PARs): Biology and Therapeutic Potential in Perioperative Stroke

Translational Stroke Research, Год журнала: 2024, Номер unknown

Опубликована: Фев. 7, 2024

Язык: Английский

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Centhaquine Increases Stroke Volume and Cardiac Output in Patients with Hypovolemic Shock

Journal of Clinical Medicine, Год журнала: 2024, Номер 13(13), С. 3765 - 3765

Опубликована: Июнь 27, 2024

Introduction: Centhaquine is a resuscitative agent that acts on α2B adrenergic receptors. Its effect cardiac output in hypovolemic shock patients has not been reported. Methods: This pilot study was conducted 12 treated with centhaquine who participated an open-label phase IV (NCT05956418). Echocardiography utilized to measure stroke volume (SV), (CO), left ventricular outflow tract velocity time integral (LVOT-VTI) and diameter (LVOTd), heart rate (HR), ejection fraction (LVEF) fractional shortening (LVFS), inferior vena cava (IVC) before (0 min) 60, 120, 300 min after (0.01 mg/kg) iv infusion for 60 min. Results: SV significantly increased CO 120 despite decrease HR. IVC LVOT-VTI at these points increased, indicating the venous return. LVEF LVFS did change, while mean arterial pressure (MAP, mmHg) Positive correlations between (R2 = 0.9556) MAP 0.8928) were observed, which indicated effects of increase return SV, CO, MAP. Conclusions: Centhaquine-mediated critical enhancing shock; changes could be pivotal reducing shock-mediated circulatory failure, promoting tissue perfusion, improving patient outcomes. Trial Registration: CTRI/2021/01/030263 NCT05956418.

Язык: Английский

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Trauma promotes heparan sulfate modifications and cleavage that disrupt homeostatic gene expression in microvascular endothelial cells

Frontiers in Cell and Developmental Biology, Год журнала: 2024, Номер 12

Опубликована: Июль 24, 2024

Heparan sulfate (HS) in the vascular endothelial glycocalyx (eGC) is a critical regulator of blood vessel homeostasis. Trauma results HS shedding from eGC, but impact trauma on structural modifications that could influence mechanisms injury and repair has not been evaluated. Moreover, effect eGC cell (EC) homeostasis fully elucidated. The objectives this work were to characterize sulfation determine transcriptional landscape ECs.

Язык: Английский

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Transitions from Aerobic to Anaerobic Metabolism and Oxygen Debt during Elective Major and Emergency Non-Cardiac Surgery

Biomedicines, Год журнала: 2024, Номер 12(8), С. 1754 - 1754

Опубликована: Авг. 5, 2024

Introduction: Intraoperative hemodynamic and metabolic optimization of both the high-risk surgical patients critically ill remains challenging. Reductions in oxygen delivery or increases consumption can initiate complex cellular processes precipitating debt (OXD). Methods: This study tested hypothesis that intraoperative changes sublingual microcirculatory flow reflect clinically relevant transitions from aerobic to anaerobic metabolism (TRANAM). We included undergoing elective major emergency non-cardiac surgery. Macro- variables, extraction, were assessed cohorts. Results: In group, OXD was progressively increased over time, with an estimated 2.24 unit increase every 30 min (adjusted p < 0.001). Also, negatively correlated central venous pressure (ρ = −0.247, adjusted 0.006) positively stroke volume variation 0.185, 0.041). However, it not significantly microcirculation variables. surgery during first two hours then gradually decreased until end cohort, diastolic arterial 0.338, adjpatients challengingsted 0.015). cardiac index −0.352, 0.003), Consensus Proportion Perfused Vessels (PPV) −0.438, 0.001), PPV (small) −0.434, Conclusions: TRANAM evident cohorts independent underlying alterations microcirculation.

Язык: Английский

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Endothelial glycocalyx degradation in critically ill foals

Journal of Veterinary Internal Medicine, Год журнала: 2024, Номер unknown

Опубликована: Сен. 14, 2024

Abstract Background Endothelial glycocalyx (EG) degradation occurs in septic humans and EG products can be used as biomarkers of endothelial injury. Information about their association with disease severity is lacking hospitalized foals. Objectives Measure serum syndecan‐1 (SDC‐1), heparan sulfate (HS), angiopoietin‐2 (ANG‐2), aldosterone (ALD), plasma atrial natriuretic peptide (ANP) concentrations to determine death Animals Ninety foals ≤3 days old. Methods Prospective, multicenter, longitudinal study. Foals were categorized into (n = 74; 55 septic; 19 sick nonseptic) 16 healthy Serum ([SDC‐1], [HS], [ANG‐2], [ALD]) measured over 72 hours using immunoassays. Results significantly higher than ( P < .05). (ANG‐2) nonsurvivors survivors On admission, (HS) > 58.7 ng/mL had odds nonsurvival (odds ratio [OR] 6.1; 95% confidence interval [CI] 1.02‐36.7). Plasma >11.5 pg/mL was associated the likelihood (OR 7.2; CI 1.4‐37.4; Septic >1018 on admission 6.5; =1.2‐36.6; Conclusion Clinical Importance Critical illness newborn injury, these are related outcome

Язык: Английский

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Centhaquine Increases Stroke Volume and Cardiac Output in Patients with Hypovolemic Shock

medRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Март 28, 2024

Abstract Background Centhaquine is a resuscitative agent that acts on α2B adrenergic receptors to enhance venous return in hypovolemic shock. The effect of centhaquine cardiac output patients with shock has not been reported. Methods Trans-thoracic echocardiography was utilized measure stroke volume (SV), (CO), left ventricular outflow tract-velocity time integral (LVOT-VTI), tract diameter (LVOTd), heart rate (HR), ejection fraction (LVEF), fractional shortening (FS) and inferior vena cava (IVC) before (0 min) after (0.01 mg/kg, iv infusion over 60 treatment (60 min, 120 300 12 randomly selected enrolled prospective, multicenter, open-label phase IV clinical study ( NCT05956418 ) Results A significant increase SV (mL) observed 60, 120, minutes treatment. CO (mL/min) increased significantly at min despite decrease HR these times. IVC LVOT-VTI points observed, which indicated return. LVEF FS did change, while the mean arterial pressure (MAP, mmHg) Positive correlations between (R 2 = 0.9556) MAP 0.8928) were mediated SV, CO, MAP. Conclusions appears play critical role enhancing shock; changes could be pivotal for reducing shock-mediated circulatory failure, promoting tissue perfusion, improving patient outcomes. Trial registration trial reported this Clinical Trials Registry, India; ctri.icmr.org.in, CTRI/2021/01/030263; clinicaltrials.gov , .

Язык: Английский

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