International Journal of Biological Macromolecules, Год журнала: 2025, Номер 297, С. 139840 - 139840
Опубликована: Янв. 13, 2025
Язык: Английский
Carbohydrate Polymer Technologies and Applications, Год журнала: 2024, Номер 8, С. 100536 - 100536
Опубликована: Июль 24, 2024
Язык: Английский
Процитировано
14
Carbohydrate Research, Год журнала: 2024, Номер 538, С. 109071 - 109071
Опубликована: Март 4, 2024
Язык: Английский
Процитировано
13
Biomacromolecules, Год журнала: 2024, Номер 25(2), С. 700 - 714
Опубликована: Янв. 31, 2024
Every 1.2 s, a diabetic foot ulcer is developed, and every 20 one amputation carried out in patients. Monitoring controlling protease activity have been considered as strategy for more efficient management of other chronic wounds. This study aimed to develop casein-based dressing that, by its disappearance, provides information about the proteases simultaneously harnesses proteolytic activity. Casein films were fabricated using an aqueous solution, heat treatment was successfully deployed green clean approach confer hydrolytic stability. Our results showed that films' mechanical characteristics, water absorption, stability could be controlled length treatment, which proved useful tool. An increase duration from 30 min 3 h led toleration 2.4 times higher stress, 2 lower uptake, 3.4 at examined conditions. Selected structures responded Bacillus sp. bacteria's (BSP) human neutrophil elastase (HNE) representatives bacterial nonbacterial found wounds 10 200 ng mL–1 levels, respectively. The hydrolysis accompanied 36% reduction measured universal assay. released casein fragments scavenge 90% radicals. In-vitro cell culture studies hydrolysates not cytotoxic, film had favorable interaction with fibroblast cells, indicating potential scaffold case would extent causes rapid disintegration. In general, these findings hold promise applying developed structure detecting without need any equipment, kits, or expertise and, importantly, highly economical manner. severe, it also serve substrate adhesion growth; this aid healing process.
Язык: Английский
Процитировано
12
View, Год журнала: 2024, Номер 5(4)
Опубликована: Июль 18, 2024
Abstract With the aging and obesity era, increasing incidence of diabetes diabetic complications, especially non‐healing wounds, imposes a serious economic burden on both patients society. The complex microenvironments, including hyperglycemia, bacterial infection, ischemia, nerve damage, lead to prolonged inflammation proliferation phase wounds. Mesenchymal stem cell‐derived small extracellular vesicles (MSC‐sEVs), which contain rich variety therapeutic molecules, have been chased for decades because their potential roles in cellular communication, tissue regeneration, drug delivery. As powerful tools controlled‐sustained release sEVs, biocompatible hydrogels applied wide range biomedical applications. Herein, we first summarize pathological features such as angiopathy, neuropathy, immune cell dysfunction. Then, discuss biological properties, performance, stability pure MSC‐sEVs. After that, components, application patterns, responsiveness hydrogels. Next, loading avenues MSC‐sEVs into hydrogel, behaviors sEVs from hydrogels, influence crosslinking method hydrogel‐sEV composites. Finally, provide an overview current applications loaded with novel cell‐free engineering system managing wounds propose critical unsolved issues. This review is expected meaningful guidance developing wound management.
Язык: Английский
Процитировано
11
Journal of Biomaterials Science Polymer Edition, Год журнала: 2024, Номер 35(5), С. 717 - 755
Опубликована: Янв. 12, 2024
Corneal diseases are a major cause of vision loss worldwide. Traditional methods like corneal transplants from donors effective but face challenges limited donor availability and the risk graft rejection. Therefore, new treatment essential. This review examines growing field bioprinting biofabrication in tissue engineering. We begin by discussing various such as stereolithography, inkjet, extrusion printing, highlighting their strengths weaknesses for eye-related uses. also explore how biological tissues made suitable through process called decellularization, which can be achieved using chemical, physical, or methods. The then looks at natural materials, known bioinks, used bioprinting. focus on materials gelatin, collagen, fibrin, chitin, chitosan, silk fibroin, alginate, examining mechanical properties. importance hydrogel scaffolds, particularly those based collagen other is discussed context repairing tissue. Another key area we cover use stem cells regeneration. pay special attention to limbal epithelial mesenchymal stromal cells, roles this process. concludes with an overview latest advancements bioprinting, early techniques advanced delivering bioengineered materials. In summary, presents current state future potential creating functional tissues, developments ongoing view towards restoring vision.
Язык: Английский
Процитировано
10
European Journal of Pharmaceutical Sciences, Год журнала: 2024, Номер 196, С. 106761 - 106761
Опубликована: Апрель 3, 2024
Inspired by nature, tissue engineering aims to employ intricate mechanisms for advanced clinical interventions, unlocking inherent biological potential and propelling medical breakthroughs. Therefore, medical, pharmaceutical fields are growing interest in organ replacement, repair, regeneration this technology. Three primary currently used engineering: transplantation of cells (I), injection growth factors (II) cellular seeding scaffolds (III). However, develop presenting highest potential, reinforcement with polymeric materials is interest. For instance, natural synthetic polymers can be used. Regardless, chitosan keratin two biopolymers great biocompatibility, biodegradability non-antigenic properties purposes offering restoration revitalization. combination has been studied results exhibit highly porous providing optimal environment cultivation. This review give an historical as well current overview engineering, involved the field.
Язык: Английский
Процитировано
10
Materials, Год журнала: 2024, Номер 17(19), С. 4792 - 4792
Опубликована: Сен. 29, 2024
Hydrogels, known for their unique ability to retain large amounts of water, have emerged as pivotal materials in both tissue engineering and biosensing applications. This review provides an updated comprehensive examination cutting-edge hydrogel technologies multifaceted roles these fields. Initially, the chemical composition intrinsic properties natural synthetic hydrogels are discussed, highlighting biocompatibility biodegradability. The manuscript then probes into innovative scaffold designs fabrication techniques such 3D printing, electrospinning, self-assembly methods, emphasizing applications regenerating bone, cartilage, skin, neural tissues. In realm biosensing, hydrogels’ responsive nature is explored through integration optical, electrochemical, piezoelectric sensors. These sensors instrumental medical diagnostics glucose monitoring, pathogen detection, biomarker identification, well environmental industrial like pollution food quality monitoring. Furthermore, explores cross-disciplinary innovations, including use wearable devices, hybrid systems, potential personalized medicine. By addressing current challenges future directions, this aims underscore transformative impact advancing healthcare practices, thereby providing a vital resource researchers practitioners field.
Язык: Английский
Процитировано
10
Bioprinting, Год журнала: 2025, Номер 45, С. e00386 - e00386
Опубликована: Янв. 5, 2025
Язык: Английский
Процитировано
2
Carbohydrate Polymers, Год журнала: 2025, Номер unknown, С. 123563 - 123563
Опубликована: Март 1, 2025
Язык: Английский
Процитировано
2
Frontiers in Bioengineering and Biotechnology, Год журнала: 2025, Номер 13
Опубликована: Фев. 12, 2025
Significant progress has been made in regenerative medicine for skin repair and rejuvenation. This review examines core technologies including stem cell therapy, bioengineered substitutes, platelet-rich plasma (PRP), exosome-based therapies, gene editing techniques like CRISPR. These methods hold promise treating a range of conditions, from chronic wounds burns to age-related changes genetic disorders. Challenges remain optimizing these therapies broader accessibility ensuring long-term safety efficacy.
Язык: Английский
Процитировано
1