Functional Peptides for Plant Disease Control

Annual Review of Phytopathology, Год журнала: 2023, Номер 61(1), С. 301 - 324

Опубликована: Июнь 2, 2023

Plant disease control requires novel approaches to mitigate the spread of and losses caused by current, emerging, re-emerging diseases adapt plant protection global climate change restrictions on use conventional pesticides. Currently, management relies mainly biopesticides, which are required for sustainable plant-protection products. Functional peptides candidate biopesticides because they originate from living organisms or synthetic analogs provide mechanisms action against pathogens. Hundreds compounds exist that cover an extensive range activities viruses, bacteria phytoplasmas, fungi oomycetes, nematodes. Natural sources, chemical synthesis, biotechnological platforms may at large scale industry growers. The main challenges their in (a) requirement stability environment counteracting resistance pathogen populations, (b) need develop suitable formulations increase shelf life methods application, (c) selection with acceptable toxicological profiles, (d) high cost production agricultural purposes. In near future, it is expected several functional will be commercially available control, but more effort needed validate efficacy field level fulfill requirements regulatory framework.

Язык: Английский

---

Cyclotides: The next generation in biopesticide development for eco‐friendly agriculture

Journal of Peptide Science, Год журнала: 2024, Номер 30(6)

Опубликована: Фев. 5, 2024

Chemical pesticides remain the predominant method for pest management in numerous countries. Given current landscape of agriculture, development biopesticides has become increasingly crucial. The strategy empowers farmers to efficiently manage pests and diseases, while prioritizing minimal adverse effects on environment human health, hence fostering sustainable management. In recent years, there been a growing interest optimism surrounding utilization peptide crop protection. These environmentally friendly substances have recognized as viable alternatives synthetic due their outstanding environmental compatibility efficacy. Numerous studies conducted synthesize identify peptides that exhibit activity against significant plant pathogens. One classes is cyclotides, which are cyclic cysteine-rich renowned wide range sequences functions. this review, we comprehensive analysis focusing structural attributes, developmental history, biological functions protection, techniques identification investigation, application biotechnology enhance cyclotide synthesis. objective emphasize considerable potential cyclotides next generation protection agents global scale.

Язык: Английский

---

Plant Elicitor Peptide (Pep) Signaling and Pathogen Defense in Tomato

Plants, Год журнала: 2023, Номер 12(15), С. 2856 - 2856

Опубликована: Авг. 3, 2023

Endogenous signaling compounds are intermediaries in pathways that plants use to respond the perception of harmful and beneficial organisms. The plant elicitor peptides (Peps) important endogenous molecules induce elements defense responses such as hormone production, increased expression defensive genes, activation phosphorelays, induction cell secondary messenger synthesis. processes by which Peps confer resistance pathogenic microorganisms have been extensively studied Arabidopsis but less known crop plants. Tomato many other solanaceous an polypeptide, systemin, is involved against herbivorous insects necrotrophic pathogens. This paper explores similarity effects chemical properties Pep systemin tomato. Additionally, relationship receptor receptors explored, identification a second tomato literature called into question. We suggest future directions for research on crops during interactions with microbes.

Язык: Английский

---

In vitro high-throughput screening of the antimicrobial activity of different compounds against Xylella fastidiosa subsp. pauca

Chemical and Biological Technologies in Agriculture, Год журнала: 2025, Номер 12(1)

Опубликована: Янв. 29, 2025

Язык: Английский

---

Structure-activity relationship of peptide conjugates derived from BP100 and insights into their interactions with lipid membranes by NMR and MD simulations

Journal of Biomolecular Structure and Dynamics, Год журнала: 2025, Номер unknown, С. 1 - 18

Опубликована: Янв. 31, 2025

Antimicrobial and plant defence elicitor peptides have received attention on last decades as novel tools to combat bacterial diseases. We previously reported a library of peptide conjugates resulting from the combination an antimicrobial (BP16, BP143, BP387 or BP475) sequence (flg15, BP13, Pep13 PIP1). From this library, we selected set 14 including both highly poorly active sequences performed structure-activity relationship study by NMR MD simulations. Analysis their structure in 30% TFE-d3 zwitterionic DPC-d38 anionic SDS-d25 micelles showed that presence α-helix fragment together with flexible random coil can be related high antibacterial activity low hemolysis. In contrast, rigid were hemolytic. PRE-NMR experiments MnCl2 16-DSA revealed flg15-BP475 BP100-Pep13 interacted stronger than BP13-BP16. two former interaction took place through region. GaMD simulations conducted membranes composed DPPG lipids, after its electrostatic interaction, flipped hydrophobic residues faced membrane triggering insertion also causing thinning. Thus, flexibility moderate cationicity seem crucial for biological activity. These findings help establish guidelines future rational design BP100 derivatives.

Язык: Английский

---

Isolation and Optimization of a Broad-Spectrum Synthetic Antimicrobial Peptide, Ap920-WI, from Arthrobacter sp. H5 for the Biological Control of Plant Diseases

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(13), С. 10598 - 10598

Опубликована: Июнь 25, 2023

Antimicrobial peptides (AMPs) are naturally occurring molecules found in various organisms that can help to defend against invading microorganisms and reduce the likelihood of drug resistance development. This study focused on isolation new AMPs from genome library a Gram-positive bacterium called

Язык: Английский

---

Synthetic peptides bioactive against phytopathogens have lower impact on some beneficial bacteria: An assessment of peptides biosafety in agriculture

Journal of Environmental Management, Год журнала: 2025, Номер 374, С. 123942 - 123942

Опубликована: Янв. 8, 2025

Язык: Английский

---

Host Defense Peptide-Mimicking Poly(2-oxazoline)s Displaying Potent Activities toward Phytopathogens to Alleviate Antimicrobial Resistance in Agriculture

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Март 26, 2025

Given the limited types of agricultural bactericides and rapid emergence antimicrobial resistance, bacterial plant diseases pose a serious threat to production, which calls for effective agents with low propensity resistance. Host defense peptides (HDPs) have drawn significant attention their broad-spectrum activity. In this study, we found that HPD-mimicking poly(2-oxazoline) Gly-POX20 exhibits potent activity against phytopathogens, superior antibacterial selectivity proteolytic stability compared natural HDP melittin. Compared commonly used bactericides, displays more efficient antibiofilm lower resistance than does antibiotic streptomycin, likely due its mechanism, involves DNA interaction generating lethal doses ROS. vivo studies reveal is in preventing treating phytopathogens without observable damage tissues, suggesting could be promising bactericide applications.

Язык: Английский

---

Preparation, characterization, antioxidant and antifungal activities of benzoic acid compounds grafted onto chitosan

International Journal of Biological Macromolecules, Год журнала: 2023, Номер 259, С. 129096 - 129096

Опубликована: Дек. 28, 2023

Язык: Английский

---

Nicotiana benthamiana as a model plant host for Xylella fastidiosa: Control of infections by transient expression and endotherapy with a bifunctional peptide

Frontiers in Plant Science, Год журнала: 2022, Номер 13

Опубликована: Дек. 1, 2022

Transient expression of genes encoding peptides BP134 and BP178 by means a Potato virus X (PVX) based-vector system, treatment with synthetic endotherapy, were evaluated in the control Xylella fastidiosa infections, model plant Nicotiana benthamiana. production significantly decreased disease severity compared to PVX non-treated (NTC) plants, without adverse effects. Plants treated showed consistently lower levels than NTC plants. However, coinfection PVX-BP134 X. caused detrimental effects resulting death. The three zones sampled, upwards downwards inoculation/treatment point, after BP178, but not when was produced transiently. effect transient induction defense-related also studied. Synthetic applied endotherapy induced ERF1, PR1a, PAL, PALII WRKY25, while overexpressed Cath, Cyc, PR4a, 9-LOX Endochitinase B genes. Both treatments upregulated PR1, PR3, PR4 CycT9299 or It concluded that either expression, on infections N. benthamiana, due part defense system addition its bactericidal activity reported previous studies. protection observed transiently seems mainly mediated defense, because affected.

Язык: Английский

---