Plasma-Activated Water Against Carbapenem-Resistant Klebsiella pneumoniae and Vancomycin-Resistant Enterococcus faecalis DOI Creative Commons
Dragana Vuković, Maja Miletić, Boško Toljić

et al.

Pathogens, Journal Year: 2025, Volume and Issue: 14(5), P. 410 - 410

Published: April 24, 2025

The scope of the antibacterial effects plasma-activated water (PAW) is not yet fully comprehended. We investigated activity PAW produced by in-house 3-pin atmospheric pressure plasma jet against carbapenem-resistant Klebsiella pneumoniae and vancomycin-resistant Enterococcus faecalis, with a focus on PAW’s potential to promote susceptibility conventional antibiotics in these bacteria. Bacterial inactivation was determined colony count after 15 60 min treatments. Minimum inhibitory concentrations (MICs) measured following repeated exposures across multiple generations bacteria enabled assessment changes antibiotics. efficacy also analyzed through detection intracellular reactive oxygen nitrogen species treated Time-dependent significant efficiency K. observed (log reduction 6.92 ± 0.24 exposure), while E. faecalis were limited. demonstrated decrease MICs crucial Namely, 50 62.5% colistin 25% vancomycin enterococci recorded. found increase superoxide anion concentration cells This study indicates that inactivating coupled capacity for potentiation antibiotic promising combination multidrug-resistant

Language: Английский

Integrating Machine Learning with MALDI-TOF Mass Spectrometry for Rapid and Accurate Antimicrobial Resistance Detection in Clinical Pathogens DOI Open Access
Xaviera A. López-Cortés, José M. Manríquez-Troncoso,

Alejandra Sepúlveda

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(3), P. 1140 - 1140

Published: Jan. 28, 2025

Antimicrobial resistance (AMR) is one of the most pressing public health challenges 21st century. This study aims to evaluate efficacy mass spectral data generated by VITEK® MS instruments for predicting antibiotic in Staphylococcus aureus, Escherichia coli, and Klebsiella pneumoniae using machine learning algorithms. Additionally, potential pre-trained models was assessed through transfer analysis. A dataset comprising 2229 spectra collected, classification algorithms, including Support Vector Machines, Random Forest, Logistic Regression, CatBoost, were applied predict resistance. CatBoost demonstrated a clear advantage over other models, effectively handling complex non-linear relationships within achieving an AUROC 0.91 F1 score 0.78 E. coli. In contrast, yielded suboptimal results. These findings highlight gradient-boosting techniques enhance prediction, particularly with from less conventional platforms like MS. Furthermore, identification specific biomarkers SHAP values indicates promising clinical applications early diagnosis. Future efforts focused on standardizing refining algorithms could expand utility these approaches across diverse environments, supporting global fight against AMR.

Language: Английский

Citations

1
Advancements in Antibacterial Therapy: Feature Papers DOI Creative Commons
Giancarlo Angeles Flores,

Gaia Cusumano,

Roberto Venanzoni

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(3), P. 557 - 557

Published: March 1, 2025

Antimicrobial resistance (AMR) is a growing global health crisis that threatens the efficacy of antibiotics and modern medical interventions. The emergence multidrug-resistant (MDR) pathogens, exacerbated by misuse in healthcare agriculture, underscores urgent need for innovative solutions. (1) Background: AMR arises from complex interactions between human, animal, environmental health, further aggravated overuse inadequate regulation antibiotics. Conventional treatments are increasingly ineffective, necessitating alternative strategies. Emerging approaches, including bacteriophage therapy, antimicrobial peptides (AMPs), nanotechnology, microbial extracellular vesicles (EVs), CRISPR-based antimicrobials, provide novel mechanisms complement traditional combating resistant pathogens. (2) Methods: This review critically analyzes advanced antibacterial strategies conjunction with systemic reforms such as stewardship programs, One Health framework, surveillance tools. These methods can enhance detection, guide interventions, promote sustainable practices. Additionally, economic, logistical, regulatory challenges impeding their implementation evaluated. (3) Results: technologies, CRISPR exhibit promising potential targeting mechanisms. However, disparities resource distribution barriers hinder widespread adoption. Public–private partnerships agriculture practices critical to overcoming these obstacles. (4) Conclusions: A holistic integrated approach essential mitigating impact AMR. By aligning therapeutic policies, fostering interdisciplinary collaboration, ensuring equitable distribution, we develop response this 21st-century challenge.

Language: Английский

Citations

1
Antiviral polymers: Broad spectrum antiviral activity, antiviral mechanisms, and optimization for the treatment of various infections DOI
Imran Hasan, Fahad A. Alharthi, Ashok Kumar Bishoyi

et al.

Journal of Industrial and Engineering Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

Citations

1
The Burden of Antimicrobial Resistance in Zambia, a Sub-Saharan African Country: A One Health Review of the Current Situation, Risk Factors, and Solutions DOI Open Access
Steward Mudenda, Webrod Mufwambi,

Shafiq Mohamed

et al.

Pharmacology &amp Pharmacy, Journal Year: 2024, Volume and Issue: 15(12), P. 403 - 465

Published: Jan. 1, 2024

Language: Английский

Citations

6
Antibacterial, antibiofilm and anti-quorum sensing activities of 1,2,3,5-tetrazine derivatives linked to a benzothiazole moiety DOI Creative Commons
Jean Paul Dzoyem, Joseph Tsemeugne, Boniface Pone Kamdem

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 15, 2025

Abstract A series of known tetrazine derivatives, containing benzothiazole scaffold, were prepared during the coupling reactions selected diazotized 2-aminobenzo[d]thiazole derivatives with p-acetaminophen. The as-prepared compounds characterized based on NMR and MS spectrometry. antibacterial anti-biofilm activities synthesized evaluated by microdilution method, whereas anti-quorum sensing effect was carried out using assay for inhibition violacein formation. As a result, 4a, 4b 4c revealed minimum inhibitory concentrations bactericidal ranging from 8 to 128 µg/mL 32 256 µg/mL, respectively. Compounds 4a (52-86.5%), (57.7-79.4%) (59.9-80.3%) prevented biofilm formation in all four bacteria tested percentages more than 50%. that inhibited 50% production found be 62.71, 28.56 107.45 respectively, thus attesting these possess activity. Noteworthy, our previous investigation attested are non-cytotoxic human mammalian cells Vero. This novel contribution demonstrates antibacterial, antibiofilm tetrazine-based benzothiazoles, which might prospected as scaffolds discovery efficient antibiotics decreased risk microbial drug resistance.

Language: Английский

Citations

0
The Leaves and Seeds of Vigna subterranean (Bambara Nut) Inhibit the Growth of Selected Bacterial and Fungal Organisms DOI Open Access

Kelechi Henry Silas,

Chizaram Winners Ndubueze,

Stanley Chukwuchebem Anorue

et al.

Asian Plant Research Journal, Journal Year: 2025, Volume and Issue: 13(1), P. 37 - 45

Published: Jan. 16, 2025

With increasing resistance of bacterial organisms to conventional antibiotics, it is crucial that alternative therapies are produced combat this menace. One plant has gathered the attention researchers in recent time as a potential medicinal Bambara nut (Vigna subterranean). Hence, study was aimed at investigating antimicrobial properties Vigna subterranea, commonly known nut, synthetic antibiotics amid rising levels. The employed an vitro experimental design, utilizing agar well diffusion method for qualitative assessment and broth microdilution determine minimum inhibitory concentration (MIC) extracts from seeds leaves plant. findings reveal both leaf seed possess significant activities against various pathogens, including Gram-positive Gram-negative bacteria fungi. comparison results showed extract higher antibacterial effect than extract. However, antifungal activity, whereas activity seen based on zones inhibition, MIC result better. Chemical analysis identified phytochemicals such flavonoids, tannins, alkaloids, which contribute observed activity. This shows subterranea potential, with potency extracts. means components could be developed natural alternatives AMR. In conclusion, these underscore importance further research into plant-based antimicrobials, may provide sustainable solutions growing challenge drug clinical settings.

Language: Английский

Citations

0
Antibiotic Resistance Gene Pollution in Poultry Farming Environments and Approaches for Mitigation: a system review DOI Creative Commons
Yunmin Chen, Yi Liu,

Zhao Cuiyan

et al.

Poultry Science, Journal Year: 2025, Volume and Issue: unknown, P. 104858 - 104858

Published: Jan. 1, 2025

Language: Английский

Citations

0
Green synthesis of silver nanoparticles using Viola odorata and Onosma hispidum: Evaluation of their antimicrobial, antioxidant, and hemolytic properties against multidrug resistant microbes DOI Creative Commons

Aneeta Andleeb,

Aneeza Khalid,

Sadia Khalil

et al.

Electronic Journal of Biotechnology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

Citations

0
Frontiers in superbug management: innovating approaches to combat antimicrobial resistance DOI
Priyanka Chambial, Neelam Thakur, Prudhvi Lal Bhukya

et al.

Archives of Microbiology, Journal Year: 2025, Volume and Issue: 207(3)

Published: Feb. 14, 2025

Language: Английский

Citations

0
Point Prevalence Survey of Antibiotic Use in Level 1 hospitals in Zambia: Future Prospects for Antimicrobial Stewardship Programs DOI Creative Commons
Steward Mudenda, Adriano Focus Lubanga, Shazia Jamshed

et al.

Infection and Drug Resistance, Journal Year: 2025, Volume and Issue: Volume 18, P. 887 - 902

Published: Feb. 1, 2025

The inappropriate prescribing and use of antibiotics have contributed to the emergence spread antimicrobial resistance (AMR). In Zambia, there is a paucity information on patterns among hospitalized patients in level 1 hospitals. This study investigated antibiotic five hospitals Lusaka, Zambia. cross-sectional utilized World Health Organization (WHO) Point Prevalence Survey (PPS) methodology in-patients admitted before 08:00 a.m. survey day August 2024. Data were analysed using IBM SPSS version 23.0. prevalence inpatients was 59.0%, with ceftriaxone being most prescribed. Antibiotics prescribed mainly for paediatrics male inpatients. found that 53.0% from Access group while 38.2% Watch Access, Watch, Reserve (AWaRe) classification. Adherence national treatment guidelines 36.0%, empirically without evidence culture sensitivity tests. high low adherence findings this demonstrate need establish strengthen stewardship programs laboratory capacity aid clinicians diagnosing, treating, managing across

Language: Английский

Citations

0