Effect of the probiotic strain, Lactiplantibacillus plantarum P9, on chronic constipation: A randomized, double-blind, placebo-controlled study

Pharmacological Research, Год журнала: 2023, Номер 191, С. 106755 - 106755

Опубликована: Апрель 3, 2023

Chronic constipation (CC) is a common gastrointestinal condition associated with intestinal inflammation, and the considerably impairs patients' quality of life. We conducted large-scale 42-day randomized, double-blind, placebo-controlled trial to investigate effect probiotics in alleviating CC. 163 patients diagnosed CC (following Rome IV criteria) were randomly divided into probiotic (n = 78; received Lactiplantibacillus plantarum P9 [P9]; 1 ×1011 CFU/day) placebo 85; material) groups. Ingesting significantly improved weekly mean frequency complete spontaneous bowel movements (CSBMs) (SBMs), while reducing level worries concerns (WO; P < 0.05). Comparing group, group was enriched potentially beneficial bacteria (Lactiplantibacillus Ruminococcus_B gnavus), depriving several bacterial phage taxa (Oscillospiraceae sp., Lachnospiraceae Herelleviridae; Interesting significant correlations also observed between some clinical parameters subjects' gut microbiome, including: negative correlation Oscillospiraceae sp. SBMs; positive WO Additionally, had (P 0.05) more predicted microbial bioactive potential involved metabolism amino acids (L-asparagine, L-pipecolinic acid), short-/medium-chain fatty (valeric acid caprylic acid). Furthermore, metabolites (p-cresol, methylamine, trimethylamine) related barrier transit decreased after administration In short, relief intervention accompanied by desirable changes fecal metagenome metabolome. Our findings support notion applying managing

Язык: Английский

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Modulating the Microbiome for Crohn’s Disease Treatment

Gastroenterology, Год журнала: 2023, Номер 164(5), С. 828 - 840

Опубликована: Янв. 24, 2023

Язык: Английский

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Microbiota perturbation by anti-microbiota vaccine reduces the colonization of Borrelia afzelii in Ixodes ricinus

Microbiome, Год журнала: 2023, Номер 11(1)

Опубликована: Июль 24, 2023

Abstract Background Ticks can transmit a broad variety of pathogens medical importance, including Borrelia afzelii , the causative agent Lyme borreliosis in Europe. Tick microbiota is an important factor modulating, not only vector physiology, but also competence. Anti-microbiota vaccines targeting keystone taxa tick alter feeding and modulate taxonomic functional profiles bacterial communities vector. However, impact anti-microbiota vaccine on tick-borne pathogen development within has been tested. Results Here, we characterized Ixodes ricinus modulation response to B. infection found that induces changes composition, its beta diversity structure community assembly. perturbation by antibodies or addition novel commensal bacteria into midguts causes departures from -induced which resulted lower load I. . Co-occurrence networks allowed identification emergent properties better defined infection-refractory states microbiota. Conclusions These findings suggest highly sensitive perturbations departure induced pose high cost spirochete. Network analysis emerges as suitable tool identify associated with states. be used for control vector-borne pathogens.

Язык: Английский

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Mechanism of Iron Ion Homeostasis in Intestinal Immunity and Gut Microbiota Remodeling

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(2), С. 727 - 727

Опубликована: Янв. 5, 2024

Iron is a vital trace element that plays an important role in humans and other organisms. It active the growth, development, reproduction of bacteria, such as Bifidobacteria. deficiency or excess can negatively affect bacterial hosts. Studies have reported major iron human intestine, which necessary for maintaining body homeostasis intestinal barrier function. Organisms maintain their normal activities regulate some cancer cells by regulating excretion iron-dependent ferroptosis. In addition, modify interaction between hosts microorganisms altering growth virulence affecting immune system host. Lactic acid bacteria Lactobacillus acidophilus (L. acidophilus), rhamnosus rhamnosus), casei casei) were to increase elements, protect host barrier, mitigate inflammation, This review article focuses on two aspects gut generally summarizes mechanistic ions immunity remodeling microbiota.

Язык: Английский

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Bacillus subtilis Simultaneously Detoxified Aflatoxin B1 and Zearalenone

Applied Sciences, Год журнала: 2024, Номер 14(4), С. 1589 - 1589

Опубликована: Фев. 16, 2024

The co-occurrence of aflatoxin B1 (AFB1) and zearalenone (ZEN) in grain-based food animal feed poses significant health risks to humans animals due their potent mutagenic, cytotoxic, carcinogenic properties. Conventional physical chemical methods are insufficient for effectively detoxifying multiple mycotoxins present feed. In this study, we evaluated the capability Bacillus subtilis ZJ-2019-1 (B. ZJ-2019-1) simultaneously degrade AFB1 ZEN while optimizing reaction enhance degradation efficiency. localization active ingredients from B. was determined using high-performance liquid chromatography (HPLC). Our findings demonstrated that eliminated 60.88% 33.18% within 72 h at a concentration 10 mg/L 37 °C (pH 7.0) exerted greater activity under alkaline conditions. autoclaved boiled supernatants exhibited enhancement ZEN, achieving rates 79.85% 100%, respectively, 1 48 °C. Moreover, crude enzymes showed maximum (100%) (94.29%) 70 Additionally, divalent cations (such as Co2+, Fe2+, Mn2+, Ni2+) significantly augmented towards mycotoxin degradation. Furthermore, when applied corn gluten meals, strain detoxify 66.08% 22.01% surpassing efficacy commercial detoxification agent on market (34.17% 2.28% ZEN). Collectively, these indicated is promising candidate simultaneous removal feed, addressing concerns associated with harmful mycotoxins.

Язык: Английский

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