Identification of novel F2-isoprostane metabolites by specific UDP-glucuronosyltransferases

Redox Biology, Год журнала: 2024, Номер 70, С. 103020 - 103020

Опубликована: Янв. 4, 2024

UDP-glucuronosyltransferases (UGTs) catalyze the conjugation of glucuronic acid with endogenous and exogenous lipophilic small molecules to facilitate their inactivation excretion from body. This represents approximately 35 % all phase II metabolic transformations. Fatty acids oxidized eicosanoid derivatives can be metabolized by UGTs. F

Язык: Английский

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Comparison of the Regenerative Metabolic Efficiency of Lipid Extracts from Microalgae Nannochloropsis oceanica and Chlorococcum amblystomatis on Fibroblasts

Antioxidants, Год журнала: 2024, Номер 13(3), С. 276 - 276

Опубликована: Фев. 24, 2024

UVA radiation leads to oxidative stress and inflammation in skin cells. Therefore, the aim of this study was compare effect lipid extracts from microalgae Nannochloropsis oceanica (N.o.) (marine) Chlorococcum amblystomatis (C.a.) (freshwater) on redox balance PUFA metabolism human fibroblasts modified by UVA. Lipid both types introduced into fibroblast medium after irradiation significantly reduced level ROS enhanced expression Nrf2, which increased activity/level antioxidants (SOD1/2, CAT, GSH, Trx). The reduction accompanied a decrease 4-HNE, its protein adducts carbonyl groups. Microalgae also activity COX1/2, FAAH MAGL UVA, as consequence, mediators (especially N.o.) decreased, group endocannabinoids (AEA, 2-AG, PEA) eicosanoids (PGE2, 15d-PGJ2, TXB2, 15-HETE), acting mainly through receptors related G protein, increases This further contributed pro-inflammatory signaling at NF-κB TNFα levels. it is suggested that N.o. C.a. can be used regenerate disturbed radiation.

Язык: Английский

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Overview of the Lipid Peroxidation Measurements in Patients by the Enzyme-Linked Immunosorbent Assay Specific for the 4-Hydroxynonenal-Protein Adducts (4-HNE-ELISA)

Frontiers in Bioscience-Landmark, Год журнала: 2024, Номер 29(4), С. 153 - 153

Опубликована: Апрель 18, 2024

Oxidative stress often affects the structure and metabolism of lipids, which in case polyunsaturated free fatty acids (PUFAs) leads to a self-catalysed chain reaction lipid peroxidation (LPO). The LPO PUFAs formation various aldehydes, such as malondialdehyde, 4-hydroxynonenal (4-HNE), 4-hydroxyhexenal, 4-oxo-2-nonenal. Among reactive 4-HNE is major bioactive product LPO, has high affinity for binding proteins. This review briefly discusses available information on applicability assessment options its protein adducts determined by immunosorbent assay (the 4-HNE-ELISA) patients with diseases known be associated oxidative stress, 4-HNE. Despite differences protocols applied antibodies used, all studies confirmed usefulness 4-HNE-ELISA research purposes. Since different used could give values when same samples, should combined other complementary analytical methods allow comparisons between obtained healthy individuals. large variations, reviewed this paper have mostly shown significantly increased levels 4-HNE-protein samples from compared As any biomarker studied patients, it preferred perform not only single-time analysis but measurements at multiple time points monitor dynamics occurrence systemic response disease causing it. especially important acute diseases, individual blood can fluctuate more than threefold within few days depending state health, was COVID-19 patients.

Язык: Английский

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Do the oxidative stress biomarkers predict COVID-19 outcome? An in-hospital cohort study

Free Radical Biology and Medicine, Год журнала: 2023, Номер 207, С. 194 - 199

Опубликована: Июль 15, 2023

Язык: Английский

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Plasma IL-17A is increased in patients with critical MIS-C and associated to in-hospital mortality

Frontiers in Immunology, Год журнала: 2025, Номер 15

Опубликована: Янв. 27, 2025

Background Multisystem inflammatory syndrome in children (MIS-C) is a rare and severe post-COVID-19 complication with multiple phenotypes. Objectives The aim of this study to biomarkers (cytokines oxidative stress) critical MIS-C patients observe if there association between these mortality. Methods A single-center prospective enrolled (with positive molecular test), aged 1 month 18 years age. Data was collected from 20 pediatric intensive care unit (PICU)’s bed. Inflammatory stress markers) were performed on day 3 after hospitalization. Survival rate calculated, Kaplan-Meier curves plotted. Univariate multivariate Cox regression analyses conducted. ROC (Receiver Operating Characteristic) curve analysis performed. Results conclusions total 41 out 109 admitted at PICU suspected during the period included, which 33 (80.5%) male, 9 (22%) under one year old, 30 (73.2%) presented comorbidities. Among them, 16 (39%) did not survive. mean survival time shorter higher levels IL-17A (≥ 19.71 pg/mL) (115 vs 323 days, p = 0.004). Higher associated mortality both crude model (HR 1.03, CI95% 1.004-1.057, 0.022) adjusted 1.043, 1.013-1.075, 0.012). revealed cut-off value for 14.32 pg/ml. other immunological markers demonstrate an (p>0.05). Our findings suggest that high are greater risk death.

Язык: Английский

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Involvement of oxidative stress in post-acute sequelae of COVID-19: clinical implications

Redox Report, Год журнала: 2025, Номер 30(1)

Опубликована: Март 3, 2025

Oxidative stress (OS) plays a key role in the pathophysiology of COVID-19 and may be associated with sequelae after severe SARS-CoV-2 infection. This study evaluated OS inflammation biomarkers blood from individuals post-acute (PASC). 64 male female participants were distributed into three groups: healthy (n = 20), acute patients (symptoms for <3 weeks, n 15), PASC >12 29). Analyses included inflammatory cytokines, myeloperoxidase (MPO) activity, markers, such as superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), gamma-glutamyl transferase (GGT), reduced (GSH), uric acid (UA), thiobarbituric reactive substances (TBARS), protein carbonyls (PC). Individuals showed increased IL-6 IL-8. Both groups exhibited decreased SOD CAT. GST only group. Elevated GGT GSH found High UA levels observed individuals. There no changes TBARS values ⁣⁣in However, PC concentrations elevated this Correlations identified between markers parameters. These findings suggest that pronounced OS, which potentially exacerbates disease complications. Monitoring could aid patient prognosis management.

Язык: Английский

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Delayed viral clearance and altered inflammatory responses affect severity of SARS-CoV-2 infection in aged mice

Immunity & Ageing, Год журнала: 2025, Номер 22(1)

Опубликована: Март 12, 2025

Epidemiological investigations consistently demonstrate an overrepresentation of the elderly in COVID-19 hospitalizations and fatalities, making advanced age as a major predictor disease severity. Despite this, comprehensive understanding cellular molecular mechanisms explaining how old represents risk factor remain elusive. To investigate we compared SARS-CoV-2 infection outcomes young adults (2 months) geriatric (15–22 mice. Both groups K18-ACE2 mice were intranasally infected with 500 TCID50 Delta variant analyses performed on days 3, 5, 7 post-infection (DPI). Analyses included pulmonary cytokines, lung RNA-seq, viral loads, lipidomic profiles, histological assessments, concurrent evaluation percentage reaching humane endpoints. The findings unveiled notable differences, aged exhibiting impaired clearance, reduced survival, failure to recover weight loss due infection. RNA-seq data suggested greater damage respiratory function Additionally, elderly-infected exhibited deficient antiviral response characterized by Th1-associated mediators (IFNγ, CCL2, CCL3, CXCL9) diminished number macrophages, NK cells, T cells. Furthermore, mass-spectrometry analysis lipidome indicated altered expression several lipids immunomodulatory pro-resolution effects such Resolvin, HOTrEs, NeuroP, but also DiHOMEs-related ARDS. These indicate that aging affects immunity, leading prolonged infection, damage, poorer clinical outcomes. This underscores potential efficacy treatments for subjects experiencing symptoms severe COVID-19.

Язык: Английский

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Redox Biomarkers – An Effective Tool for Diagnosing COVID-19 Patients and Convalescents

Journal of Inflammation Research, Год журнала: 2024, Номер Volume 17, С. 2589 - 2607

Опубликована: Апрель 1, 2024

Aim: COVID-19 triggers the overproduction of reactive oxygen species (ROS) which, in combination with a weakened antioxidant barrier, can lead to protein oxidation and lipid peroxidation. The aim this study was evaluate enzymatic non-enzymatic antioxidants, overall redox potential, peroxidation products patients, convalescents, healthy subjects, determine diagnostic applicability these parameters patients. Materials Methods: involved 218 patients COVID-19, 69 48 subjects who were selected for research based on age sex. conducted between 20 February 2021 November Białystok, Poland. status, oxidative damage assessed serum/plasma samples use colorimetric spectrophotometric assays. Results: Glutathione reductase (GR) activity higher, whereas total capacity (TAC) lower than convalescents (p< 0.0001) control group 0.0001). concentrations advanced glycation end (AGEs), (AOPP), 4-hydroxynonenal (4-HNE), malondialdehyde (MDA) higher group. AGEs most effective biomarker differentiating from (AUC=0.9971) (AUC=1.000). Conclusion: An infection SARS-CoV-2 disrupts balance increases fulfill criteria potential convalescents. Keywords: stress, biomarkers, SARS CoV-2 virus

Язык: Английский

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Role of different types of RNA molecules in the severity prediction of SARS-CoV-2 patients

Pathology - Research and Practice, Год журнала: 2023, Номер 242, С. 154311 - 154311

Опубликована: Янв. 15, 2023

Язык: Английский

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An Update on Glutathione's Biosynthesis, Metabolism, Functions, and Medicinal Purposes

Current Medicinal Chemistry, Год журнала: 2023, Номер 31(29), С. 4579 - 4601

Опубликована: Ноя. 6, 2023

Glutathione (GSH) has been the focus of increased scientific interest in last decades. It plays a crucial role all major physiological processes by supplying antioxidant defenses through participating cellular redox reactions human body and other living organisms. GSH also participates detoxifying xenobiotics, protecting protein thiols from crosslinking oxidation, regulating cell cycle, storing cysteine, etc. The significant most important highlighted, such as maintaining balance reducing oxidative stress due to its ability inactivate reactive oxygen, nitrogen, sulfur species. can enhance metabolic detoxification regulate function immune system. All these characteristics make it universal biomarker since proper is essential for improving health treating some age-related disorders. This review presents current concept synthesis metabolism GSH; main functions organism, precursor cofactor; data on use medicinal purposes prevention treatment diseases, well nutritional strategy maintain normal pool body. were gathered searching relevant information multiple databases, PubMed, Scopus, ScienceDirect, Google Scholar.

Язык: Английский

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