Influence of extraction solvents on total phenolic content and antioxidant activity of Tunisian Thymbra capitata (L.) Cav. aerial parts extracts

Journal of Phytomolecules and Pharmacology, Год журнала: 2025, Номер 4(1), С. 07 - 12

Опубликована: Апрель 23, 2025

Язык: Английский

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Assessing the Chemical Composition, Antioxidant and enzyme Inhibitory Effects of Pentapleura subulifera and Cyclotrichium glabrescens Extracts

Chemistry & Biodiversity, Год журнала: 2023, Номер 21(2)

Опубликована: Ноя. 28, 2023

Abstract The Lamiaceae family, encompassing diverse plant species, holds significant value in food, medicine, and cosmetics. Within this Pentapleura subulifera Cyclotrichium glabrescens , relatively unexplored were investigated for their chemical composition, antioxidant capacity, enzyme‐inhibiting effects. composition of hexane, methanolic, aqueous extracts from P. C. analyzed using LC‐ESI‐MS/MS the non‐polar hexane fraction was via GC‐MS. potential determined through radical scavenging, reducing power metal chelating assays. Additionally, inhibitory activity against six enzymes – acetylcholinesterase (AChE), butyrylcholinesterase (BChE), tyrosinase, amylase, glucosidase examined. extract methanolic exhibited elevated phenolic content at 129.47 mg gallic acid equivalent (GAE)/g 55.97 GAE/g, respectively. Chemical profiling constituents two species resulted identification a total twenty compounds. majority which belonged to flavonoids quinic derivatives, primarily concentrated methanol extracts. Among all assays, demonstrated superior activity, with highest recorded 404.93 trolox (TE)/g cupric capacity (CUPRAC) test. Meanwhile, AChE 2.71 galanthamine (GALAE)/g, followed by 2.41 GALAE/g. These findings unequivocally establish notable enzyme extracts, underscoring as source valuable natural antioxidants.

Язык: Английский

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Effect of Thymbra capitata (L.) Cav. on Inflammation, Senescence and Cell Migration

Nutrients, Год журнала: 2023, Номер 15(8), С. 1930 - 1930

Опубликована: Апрель 17, 2023

Aromatic plants are reported to display pharmacological properties, including anti-aging. This work aims disclose the anti-aging effect of essential oil (EO) Thymbra capitata (L.) Cav., an aromatic and medicinal plant widely used as a spice, well hydrodistillation residual water (HRW), discarded by-product EO hydrodistillation. The phytochemical characterization HRW was assessed by GC-MS HPLC-PDA-ESI-MSn, respectively. DPPH, ABTS, FRAP assays were antioxidant properties. anti-inflammatory potential evaluated using lipopolysaccharide-stimulated macrophages assessing NO production, iNOS, pro-IL-1β protein levels. Cell migration scratch wound assay, etoposide-induced senescence assess modulation senescence. is mainly characterized carvacrol, while predominantly rosmarinic acid. exerts stronger in DPPH assays, whereas most active sample ABTS assay. Both extracts reduce NO, pro-IL-1β. has no on cell presents anti-senescence effects. In opposition, reduces induces cellular Overall, our study highlights interesting properties for both extracts, being interest ingredient relevant cancer therapy.

Язык: Английский

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Multidirectional research for the therapeutic potential of Phlomoides molucelloides (Bunge) Salmaki: LC-MS/MS, antioxidant, enzyme inhibition, and antiproliferative characteristics

Process Biochemistry, Год журнала: 2024, Номер 143, С. 302 - 314

Опубликована: Май 10, 2024

Язык: Английский

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Nutrient Deficiency-Induced Stress Improves Skincare Effects and Phytochemical Content of Green Extracts from Lamiaceae In Vitro Cultures

Horticulturae, Год журнала: 2024, Номер 10(9), С. 947 - 947

Опубликована: Сен. 5, 2024

The objective of this study was to investigate the impact nutrient starvation on growth, biochemical, metabolomic, and biological traits Lavandula viridis L’Hér Thymus lotocephalus G. López R. Morales in vitro cultures. In both species, a reduction shoot growth production chlorophyll carotenoids observed cultures grown under nutrient-deficient media (especially Fe N) compared those control conditions. highest levels hydrogen peroxide lipid peroxidation, two indicators oxidative stress, were L. N deficiency T. P limitation. results demonstrated that led 72% 62% increase quantified phenolic compounds lotocephalus, respectively. concentrations major compound species—rosmarinic acid—were Mg-deficient (60.7 ± 1.0 mg/gDW) Fe-deficient (50.0 0.4 conditions Furthermore, enhanced capacity green extracts inhibit three enzymes (tyrosinase, elastase, hyaluronidase) associated with anti-aging their antioxidant properties.

Язык: Английский

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Comprehensive metabolite and biological profile of “Sulmona Red Garlic” ecotype’s aerial bulbils

Food Research International, Год журнала: 2023, Номер 175, С. 113654 - 113654

Опубликована: Ноя. 2, 2023

"Sulmona Red Garlic" is a well-known Italian traditional product. Bulbs, used for culinary purposes, have been largely investigated their medicinal properties whereas aerial bulbils are usually removed as waste material. Here, the first time, chemical composition and biological of hydroalcoholic extract from were investigated. Complementary information on metabolite obtained using both NMR based untargeted HPLC-DAD targeted methodologies. The analysis revealed presence sugars, organic acids, amino organosulphur compounds (methiin, alliin, allicin cycloalliin), other secondary metabolites. In particular, methiin alliin identified time in spectra bulbil garlic extracts. Polyphenol content was determined by analysis: catechin, chlorogenic acid, gallic acid turned out to be most abundant phenolics. Hydroalcoholic blocked cell proliferation colon cancer line HCT116 with an IC50 352.07 µg/mL, while it non-toxic myoblast C2C12. addition, caused seedling germination reduction two edible herbaceous dicotyledon species, namely Cichorium intybus C. endivia. Moreover, same reduced gene expression TNF-α (tumor necrosis factor), HIF1-α (hypoxia-inducible VEGFA (vascular endothelial growth transient receptor potential (TRP) M8 (TRPM8) indicating ability contrast development through angiogenic pathway. Final, silico experiments also carried supporting effects compounds, particularly which may directly interact TRPM8. results here reported suggest use often considered product source phytotherapeutic remedies.

Язык: Английский

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Above the Invasive and Ornamental Attributes of the Traveler’s Palm: An In Vitro and In Silico Insight into the Anti-Oxidant, Anti-Enzymatic, Cytotoxic and Phytochemical Characterization of Ravenala madagascariensis

Antioxidants, Год журнала: 2023, Номер 12(1), С. 184 - 184

Опубликована: Янв. 12, 2023

Ravenala madagascariensis is a widely known ornamental and medicinal plant, but with dearth of scientific investigations regarding its phytochemical pharmacological properties. Hence, these properties were appraised in this study. The DPPH (154.08 ± 2.43 mgTE/g), FRAP (249.40 3.01 CUPRAC (384.57 1.99 metal chelating (29.68 0.74 mgEDTAE/g) phosphomolybdenum assay (2.38 0.07 mmolTE/g) results demonstrated that the aqueous extract had most prominent antioxidant activity, while methanolic displayed best potential ABTS (438.46 1.69 mgTE/g). HPLC-ESI-Q-TOF-MS-MS analysis allowed characterization 41 metabolites. was active against acetylcholinesterase. All extracts alpha-amylase alpha-glucosidase enzymes, ethyl acetate being enzyme, showed inhibition. A plethora metabolites bonded more energetically assayed enzymes sites based on silico studies. R. used study exhibited cytotoxicity HT29 cells. IC50 lower (506.99 ug/mL). Based heat map, whereby flavonoids found to be greater proportion extracts, it can concluded flavonoid portion contributed activity.

Язык: Английский

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Comparative Study on the Chemical Profile, Antioxidant Activity, and Enzyme Inhibition Capacity of Red and White Hibiscus sabdariffa Variety Calyces

ACS Omega, Год журнала: 2023, Номер 8(45), С. 42511 - 42521

Опубликована: Окт. 30, 2023

Hibiscus sabdariffa L. (Family: Malvaceae) is believed to be domesticated by the people of western Sudan sometime before 4000 BC for their nutritional and medicinal properties. This study aimed investigate chemical profile, antioxidant activity, enzyme inhibition property extracts from red roselle (RR) white (WR) varieties grown in Sudan. Three aqueous obtained maceration, infusion, decoction, addition methanolic one, were prepared two varieties. Results showed that highest total phenolic flavonoid contents RR infusion (28.40 mg GAE/g) decoction (7.94 RE/g) respectively, while those WR recorded extract (49.59 GAE/g 5.81 RE/g respectively). Extracts mainly characterized high accumulation chlorogenic acid (6502.34–9634.96 kg–1), neochlorogenic (937.57–8949.61 gallic (190–4573.55 kg–1). On other hand, (1777.05–6946.39 kg–1) rutin (439.29–2806.01 dominant compounds WR. All had significant (p < 0.05) higher activity than respective except metal chelating power, where latter (3.87 EDTAE/g). values (35.09, 52.17, 65.62, 44.92 TE/g) DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), CUPRAC (cupric ion reducing capacity), FRAP (ferric power) assays, respectively. exerted acetylcholinesterase (AChE) inhibitory (3.42–4.77 GALAE/g; GALAE = galantamine equivalents), only one extract, AChE (4.79 GALAE/g). relatively BChE (butyrylcholinesterase) (3.71–4.23 GALAE/g) ones. Methanolic displayed Tyr (tyrosinase) (RR 48.25 KAE/g; 42.71 KAE/g). The exhibited amylase (0.59 mmol ACAE/g) glucosidase (1.46 activity. Molecular docking analysis delphinidin 3,5-diglucoside, rutin, isoquercitrin, hyperoside, most promising effect. In conclusion, these findings indicated profiles biological varied according variety, extraction solvent, technique used. These can serve as a valuable source development multiple formulations food, pharmaceutical, cosmetic industries.

Язык: Английский

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An analytical framework combining online high‐performance liquid chromatography methodologies and biological properties of different extracts of Leonurus cardiaca

Journal of Separation Science, Год журнала: 2023, Номер 47(1)

Опубликована: Дек. 3, 2023

Little or no information is available concerning online high‐performance liquid chromatography (HPLC) antioxidants and the antibiofilm effect of Leonurus cardiaca . Five distinct extractions methanolic, ethyl acetate, dichloromethane, hexane, water were obtained from L. In online‐HPLC‐antioxidant analysis all examined samples, rosmarinic acid emerged as primary antioxidant, registering concentrations ranging 6 to 15 ppm at wavelengths 517 734 nm. Notably, extract exhibited robust antioxidant activity vitro. Regarding acetylcholinesterase butrylcholinesterase inhibition, n‐hexane superior inhibition with values 3.08 5.83 galanthamine equivalent, respectively. Except for extract, tested extracts (at a concentration 20 μg/mL) substantial inhibitory against biofilm formation, in many cases 80%, reached even 94.52% Escherichia coli Although less vigorous, also acted mature (inhibition up 76.50% Staphylococcus aureus ). They could work metabolism inside an immature biofilm, percentages 93.18% (vs. Pseudomonas aeruginosa ) Acinetobacter baumannii ), Considering its significant antioxidants, enzyme antimicrobial activity, emerges promising candidate therapeutic potential.

Язык: Английский

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Evaluating Phytochemical Profiles, Cytotoxicity, Antiviral Activity, Antioxidant Potential, and Enzyme Inhibition of Vepris boiviniana Extracts

Molecules, Год журнала: 2023, Номер 28(22), С. 7531 - 7531

Опубликована: Ноя. 10, 2023

In the present study, we performed comprehensive LC-MS chemical profiling and biological tests of Vepris boiviniana leaves stem bark extracts different polarities. total, 60 bioactive compounds were tentatively identified in all extracts. The 80% ethanolic extract exhibited highest activity ABTS assay, equal to 551.82 mg TE/g. infusion consistently demonstrated elevated antioxidant assays, with values ranging from 137.39 TE/g 218.46 Regarding enzyme inhibitory aqueous both substantial inhibition AChE, EC50 2.41 GALAE/g 2.25 GALAE/g, respectively. leaf lowest cytotoxicity VERO cells (CC50: 613.27 µg/mL) selective against cancer cells, particularly H1HeLa indicating potential therapeutic specificity. toxicity but had reduced anticancer selectivity. n-hexane extracts, notably leaves' extract, displayed towards non-cancerous selectivity RKO cells. viral load assessment, HHV-1 by 0.14-0.54 log HRV-14 0.13-0.72 log, limited antiviral activity. conclusion, our research underscores diverse properties exhibiting potent antioxidant, inhibitory,

Язык: Английский

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