Extracellular Vesicles Modulate Liver Cells Viability and Reactive Oxygen Species in Patients Following a Very Low-Calorie Ketogenic Diet

Nutrients, Год журнала: 2024, Номер 16(15), С. 2386 - 2386

Опубликована: Июль 23, 2024

The VLCKD is a diet recognized to promote rapid fat mobilization and reduce inflammation, hepatic steatosis, liver fibrosis. Extracellular vesicles (EVs) mediate cell-to-cell communication. aim of the study investigate role circulating EVs in cell proliferation, ketone bodies, ROS production patients on an 8-week regimen. Participants were classified as responders (R) or non-responders (NR) treatment based their fibroscan results. In vitro experiments with lines HEPA-RG (normal hepatocytes) LX-2 (stellate cells) conducted effects viability, production, body presence. findings reveal notable reduction viability both when treated exosomes (EXOs). contrast, microvesicles (MVs) did not appear affect which remained unchanged. Additionally, levels bodies measured urine consistently correlated fibrosis (R). Similarly, increase was observed (NR), also aligned expected This inconsistency stands stark contrast Reactive Oxygen Species (ROS), exhibited clear consistent pattern accordance dietary intervention. Finally, this preliminary study, has been identified potential adherence marker for patients; reliably follow progression response, providing more accurate reflection therapeutic effects.

Язык: Английский

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Inflammaging: The Next Challenge—Exploring the Role of Gut Microbiota, Environmental Factors, and Sex Differences

Biomedicines, Год журнала: 2024, Номер 12(8), С. 1716 - 1716

Опубликована: Авг. 1, 2024

The term 'inflammaging' has been coined to describe the chronic state of inflammation derived from ongoing cycles tissue damage and subsequent immune responses. This inflammatory status contributes decline organs physiological functions, accelerates aging process, increases risk age-related illnesses death. During aging, gut microbiota (GM) undergoes significant changes, including a decreased diversity species, in beneficial bacteria, rise proinflammatory ones, resulting persistent low-grade inflammation. Moreover, environmental factors, such as diet medications, contribute changes GM function, preventing or promoting inflammaging. narrative review aims clarify underlying mechanisms inflammaging specifically investigate influence several factors on these mechanisms, while also exploring potential differences related sex. lifestyle pharmacological interventions will be suggested promote healthy aging.

Язык: Английский

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Use of a Micronutrient Cocktail to Improve Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD) in Adults with Obesity: A Randomized, Double-Blinded Pilot Clinical Trial

Medicina, Год журнала: 2024, Номер 60(8), С. 1366 - 1366

Опубликована: Авг. 21, 2024

Background and Objectives: The goal of this study was to assess the impact supplementation with a combination nutrients on metabolic-dysfunction-associated steatotic liver disease (MASLD)-related parameters, other parameters related metabolic syndrome in adults obesity. These measurements included anthropometric lipid profiling, FibroScan technology (controlled attenuation parameter (CAP) transient elastography (TE) values). Materials Methods: A double-blind, placebo-controlled pilot clinical trial conducted over three-month treatment period. Adults obesity were allocated receive either cocktail defined daily dosages (5-MTHF, betaine, alpha-linolenic acid, eicosapentaenoic choline bitartrate, docosahexaenoic vitamin B12) or placebo. participants evaluated at start end Results: total 155 entered study, comprising 84 group 71 placebo group. administration nutritional supplement resulted notable reduction both CAP TE scores when compared exhibited mean 4% (p < 0.05) 7.8% 0.05), indicative decline fat content fibrosis. Conclusions: period three months led significant amelioration fibrosis steatosis findings suggest that regimen could be beneficial adjunct therapy for improving health obesity-induced MASLD.

Язык: Английский

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Preventive effect of low-carbohydrate high-fat dietary pattern on liver disease caused by alcohol consumption via a 6pgd-involved mechanism in mice

Food & Function, Год журнала: 2023, Номер 15(2), С. 732 - 746

Опубликована: Дек. 7, 2023

A low-carbohydrate high-fat (LCHF) dietary pattern has been reported to improve chronic metabolic diseases. However, whether and how the LCHF diet affects pathological progression in patients with alcohol-related liver diseases (ALD) is largely unknown. This study was conducted evaluate effect of on ALD clarify its potential mechanism(s). The model established by feeding C57BL/6N mice a Lieber-DeCarli liquid alcohol modified carbohydrate/fat ratio under an isoenergetic pattern. After eight-week intervention, we observed that significantly reduced alcohol-induced hepatic steatosis injury, along improved lipid metabolic-related gene disorders redox imbalance. alcohol-stimulated increase pro-inflammatory cytokine cytokines expression, including TNF-α, IL-1β, IL-6, markedly reversed diet. Liver transcriptome sequencing qPCR validation showed twenty-four alcohol-disturbed genes were LCHF-diet intervention. top differentially expressed selected for further investigation. Among them, 6-phosphogluconate dehydrogenase (6PGD) up-regulated treatment both cultured hepatocytes. Spearman correlation analysis revealed 6PGD positively associated steatosis, oxidative stress indexes. In vitro, knockdown ameliorated hepatotoxicity intracellular accumulation, as well disorders, implying involvement LCHF-protected ALD. conclusion, intervention alleviated consumption-induced dysfunction mice. novel target prevention contributes LCHF-improved might be promising choice management.

Язык: Английский

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Take the bull by the horns and tackle the potential downsides of the ketogenic diet

Nutrition, Год журнала: 2024, Номер 125, С. 112480 - 112480

Опубликована: Май 2, 2024

Язык: Английский

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