Sugar Beet (Beta vulgaris) Guard Cells Responses to Salinity Stress: A Proteomic Analysis

International Journal of Molecular Sciences, Год журнала: 2020, Номер 21(7), С. 2331 - 2331

Опубликована: Март 27, 2020

Soil salinity is a major environmental constraint affecting crop growth and threatening global food security. Plants adapt to by optimizing the performance of stomata. Stomata are formed two guard cells (GCs) that morphologically functionally distinct from other leaf cells. These microscopic sphincters inserted into wax-covered epidermis shoot balance CO2 intake for photosynthetic carbon gain concomitant water loss. In order better understand molecular mechanisms underlying stomatal function under saline conditions, we used proteomics approach study isolated GCs salt-tolerant sugar beet species. Of 2088 proteins identified in GCs, 82 were differentially regulated salt treatment. According bioinformatics analysis (GO enrichment protein classification), these involved lipid metabolism, cell wall modification, ATP biosynthesis, signaling. Among significant abundant proteins, several classified as “stress proteins” upregulated, including non-specific transfer protein, chaperone heat shock inorganic pyrophosphatase 2, responsible energized vacuole membrane ion transportation. Moreover, antioxidant enzymes (peroxide, superoxidase dismutase) highly upregulated. Furthermore, detected provided some evidence GC walls more flexible response stress. Proteins such L-ascorbate oxidase constitutively high both control conditions may contribute ability mitigating salinity-induced oxidative

Язык: Английский

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Synthetic Oligopeptides Mimicking γ-Core Regions of Cysteine-Rich Peptides of Solanum lycopersicum Possess Antimicrobial Activity against Human and Plant Pathogens

Current Issues in Molecular Biology, Год журнала: 2021, Номер 43(3), С. 1226 - 1242

Опубликована: Сен. 24, 2021

Plant cysteine-rich peptides (CRPs) represent a diverse group of molecules involved in different aspects plant physiology. Antimicrobial peptides, which directly suppress the growth pathogens, are regarded as promising templates for development next-generation pharmaceuticals and ecologically friendly disease control agents. Their oligopeptide fragments even more because their low production costs. The goal this work was to explore antimicrobial activity nine short derived from γ-core-containing regions tomato CRPs against important human pathogens. We discovered defensin-like snakins, MEG, demonstrates direct involvement these defense reactions tomato. CRP-derived appeared particularly active gram-positive bacterium Clavibacter michiganensis, causes bacterial wilt—opening up new possibilities use agriculture dangerous disease. Furthermore, high inhibitory potency oligopeptides demonstrated yeast Cryptococcus neoformans, serious diseases humans, making peptide candidates pharmaceuticals. Studies mode action two most indicate fungal membrane permeabilization mechanism action.

Язык: Английский

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The γ-Core Motif Peptides of AMPs from Grasses Display Inhibitory Activity against Human and Plant Pathogens

International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(15), С. 8383 - 8383

Опубликована: Июль 29, 2022

Antimicrobial peptides (AMPs) constitute an essential part of the plant immune system. They are regarded as alternatives to conventional antibiotics and pesticides. In this study, we have identified γ-core motifs, which associated with antimicrobial activity, in 18 AMPs from grasses assayed their properties against nine pathogens, including yeasts affecting humans, well pathogenic bacteria fungi. All tested displayed properties. We discovered a number short AMP-derived high activity both human pathogens. For first time, was revealed designed 4-Cys-containing defensin-like peptides, whose role immunity has remained unknown, knottin-like peptide C-terminal prodomain thionin, points direct involvement these defense mechanisms. Studies mode action eight most active motif on yeast cells using staining propidium iodide showed that all them induced membrane permeabilization leading cell lysis. addition identification determinants AMPs, work provides candidate molecules for development novel drugs effective opportunistic fungal infections biopesticides control

Язык: Английский

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Non-specific Lipid Transfer Proteins in Legumes and Their Participation During Root-Nodule Symbiosis

Frontiers in Agronomy, Год журнала: 2021, Номер 3

Опубликована: Апрель 22, 2021

Non-specific lipid transfer proteins (LTPs) constitute a large protein family in plants characterized by having tunnel-like hydrophobic cavity, which allows them to different molecules. LTPs have been studied various model including those of agronomic interest. Recent studies demonstrated that play key functions both biotic and abiotic stress. In plants-pathogen interaction, they act as either positive or negative regulators defense responses. However, little is known about the roles symbiotic interactions, especially root nodule symbiosis. Here, we performed broad genome analysis LTP members legumes other important plants, focusing on their possible legume-rhizobium silico showed contain families, with at least 70 clustered into four clades. Although structures genes are conserved among species, differences were observed between clades from species. widely expressed plant tissues. general, LTP1 LTP2 classes highly shoot reproductive tissues all analyzed Furthermore, also roots inoculated rhizobia nodules legumes. RT-qPCR expression profile seven PvLTP common bean ( Phaseolus vulgaris ) revealed these differentially during early late stages nodulation genetically regulated PvRbohA . These findings provide insight putative symbiosis interactions RBOH-dependent ROS production.

Язык: Английский

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Characterization of non-specific lipid transfer protein (nsLtp) gene families in the Brassica napus pangenome reveals abundance variation

BMC Plant Biology, Год журнала: 2022, Номер 22(1)

Опубликована: Янв. 7, 2022

Brassica napus is an important agricultural species, improving stress resistance was one of the main breeding goals at present. Non-specific lipid transfer proteins (nsLTPs) are small, basic which involved in some biotic or abiotic responses. B. susceptible to a variety fungal diseases, so identify BnLTPs and their expression disease responses very important. The common reference genome does not contain all genes because gene presence/absence variations between individuals. Therefore, it necessary search for candidate BnLTP pangenome.In present study, were identified throughout pangenome, different presented among varieties. Totally, 246 could be divided into five types (1, 2, C, D, G). classification, phylogenetic reconstruction, chromosome distribution, functional annotation, analyzed. We also potential cis-elements that respond stresses 2 kb upstream regions genes. RNA sequencing analysis showed response Sclerotinia sclerotiorum infection. 32 linked blackleg quantitative trait locus (QTL).The identification LTP pangenome help elucidate function family members provide new information future molecular napus.

Язык: Английский

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Inhibition of Serine Protease, α-Amylase and Growth of Phytopathogenic Fungi by Antimicrobial Peptides from Capsicum chinense Fruits

Probiotics and Antimicrobial Proteins, Год журнала: 2021, Номер 15(3), С. 502 - 515

Опубликована: Окт. 20, 2021

Язык: Английский

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The Influence of Biomolecule Composition on Colloidal Beer Structure

Biomolecules, Год журнала: 2021, Номер 12(1), С. 24 - 24

Опубликована: Дек. 24, 2021

Recent studies have revealed an interest in the composition of beer biomolecules as a colloidal system and their influence on formation taste. The purpose this research was to establish biochemical interactions between plant-based raw materials order understand overall structure complex bound biomolecules. Generally accepted methods analytical field brewing, biochemistry proteomics were used solve objectives. allowed us relationship grain used, well processing technologies biomolecular profiles beer. qualitative profile distribution protein compounds framework for role carbohydrate dextrins phenol are given. This article provides information about presence biogenic that positively affect functioning body. A critical assessment some parameters completeness taste by is Conclusion: conducted confirm hypothesis nitrogen other with substances, identify main factors affecting fractions.

Язык: Английский

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Structural and Functional Characterization of New Lipid Transfer Proteins with Chitin-Binding Properties: Insights from Protein Structure Prediction, Molecular Docking, and Antifungal Activity

Biochemistry, Год журнала: 2024, Номер 63(14), С. 1824 - 1836

Опубликована: Июль 5, 2024

Faced with the emergence of multiresistant microorganisms that affect human health, microbial agents have become a serious global threat, affecting health and plant crops. Antimicrobial peptides attracted significant attention in research for development new control agents. This work's goal was structural characterization analysis antifungal activity chitin-binding from

Язык: Английский

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Complex of Defense Polypeptides of Wheatgrass (Elytrigia elongata) Associated with Plant Immunity to Biotic and Abiotic Stress Factors

Plants, Год журнала: 2024, Номер 13(17), С. 2459 - 2459

Опубликована: Сен. 3, 2024

Plant defense polypeptides play a crucial role in providing plants with constitutive immunity against various biotic and abiotic stressors. In this study, we explored complex of proteins from wheatgrass (Elytrigia elongata) spikelets to estimate their the plant’s tolerance environmental factors. The current research shows that vitro protein extracts E. elongata possess antifungal activity certain Fusarium species, which are specific cereal pathogens, at concentrations 1–2 mg/mL. reproduced these activities using 4 mg/mL extract artificial fungal infection experiments on wheat grain (Triticum aestivum) under controlled laboratory conditions. Furthermore, tested demonstrated protective effect Saccharomyces cerevisiae exposed hyper-salinity stress concentration 2 A combined scheme fractionation structural identification was applied for estimation diversity polypeptides. Defensins, lipid-transfer proteins, hydrolase inhibitors (cereal bifunctional trypsin/alpha-amylase Bowman–Birk trypsin inhibitor), high-molecular-weight disease resistance were isolated extract. Thus, appear be reservoir Our findings contribute deeper understanding plant peptides involvement adaptation factors, they reveal regulatory ecosystem level.

Язык: Английский

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Cysteine-Rich Peptide Genes of the Wheatgrass Thinopyrum elongatum

Russian Journal of Genetics, Год журнала: 2024, Номер 60(10), С. 1341 - 1354

Опубликована: Окт. 1, 2024

Язык: Английский

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