Killing softly: a roadmap of Botrytis cinerea pathogenicity

Trends in Plant Science, Год журнала: 2022, Номер 28(2), С. 211 - 222

Опубликована: Сен. 30, 2022

Botrytis cinerea, a widespread plant pathogen with necrotrophic lifestyle, causes gray mold disease in many crops. Massive secretion of enzymes and toxins was long considered to be the main driver infection, but recent studies have uncovered rich toolbox for B. cinerea pathogenicity. The emerging picture is multilayered infection process governed by exchange factors that collectively contribute development. No shows complete resistance against pattern-triggered immune responses potential significantly reduce progression, opening new possibilities producing cinerea-tolerant plants. We examine current models, highlight knowledge gaps, suggest directions future studies.

Язык: Английский

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Modified chitosan: Insight on biomedical and industrial applications

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 275, С. 133526 - 133526

Опубликована: Июль 2, 2024

Язык: Английский

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Antifungal Activity of Novel Indole Derivatives Containing 1,3,4-Thiadiazole

Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер 72(18), С. 10227 - 10235

Опубликована: Апрель 26, 2024

In this study, 24 indole derivatives containing 1,3,4-thiadiazole were discovered and synthesized. The target compounds' antifungal efficacy against 14 plant pathogenic fungal pathogens was then determined in vitro. With an EC50 value of 2.7 μg/mL, Z2 demonstrated the highest level bioactivity among them Botrytis cinerea (B.c.), exceeding concentrations control prescription drugs azoxystrobin (Az) (EC50 = 14.5 μg/mL) fluopyram (Fl) 10.1 μg/mL). underwent vivo testing on blueberry leaves order to evaluate its usefulness real-world settings. A reasonable protective effect obtained with a effectiveness 93.0% at 200 which superior those Az (83.0%) Fl (52.0%). At chemical had 84.0% terms curative efficacy. These figures outperformed (69.0%) (48.0%). Scanning electron microscopy (SEM) experiments light showed that altered integrity cell wall membrane fungus B.c., led increase content malondialdehyde (MDA), cellular leakage, permeability. Enzyme activity assays molecular docking studies indicated could act as potential succinate dehydrogenase inhibitor (SDHI). It hypothesized cause disruption mycelial membranes, turn leads death. According research, expected evolve into new fungicides due their significant effects fungi.

Язык: Английский

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Eco-friendly management of postharvest fungal decays in kiwifruit

Critical Reviews in Food Science and Nutrition, Год журнала: 2021, Номер 62(30), С. 8307 - 8318

Опубликована: Май 17, 2021

Kiwifruit is purchased by consumers worldwide and increasing in demand. Unfortunately, kiwifruit susceptible to postharvest decay caused a variety of fungal pathogens, including Botrytis cinerea, Penicillium expansum, Alternaria alternata, Botryosphaeria dothidea, Diaporthe spp. Among these B. cinerea the most prevalent devastating. Infections pathogens result deterioration fruit quality reduction marketable yield. Eco-friendly methods control have been explored as alternatives use synthetic fungicides. In this review, we provide an overview discuss virulence pathogenesis fungi that are causal agents decay, especially recent molecular genomic studies. Advances pre- measures for management, biological control, physical applications, natural compounds plant hormones, combined methods, also reviewed. critical component integrated management approach sustainable production kiwifruit. The need further research on microbial consortia diseases discussed.

Язык: Английский

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A novel approach to control Botrytis cinerea fungal infections: uptake and biological activity of antifungals encapsulated in nanoparticle based vectors

Scientific Reports, Год журнала: 2022, Номер 12(1)

Опубликована: Май 14, 2022

Botrytis cinerea, responsible for grey mold diseases, is a pathogen with broad host range, affecting many important agricultural crops, in pre and post harvesting of fruits vegetables. Commercial fungicides used to control this are often subjected photolysis, volatilization, degradation, leaching, runoff during application. In context, the use delivery system, based on poly (lactic-co-glycolic acid) nanoparticles (PLGA NPs) represents an innovative approach develop new pesticide formulations successfully fight B. cinerea infections. order study NPs uptake, conidia mycelium were treated PLGA loaded high fluorescent probe coumarin 6 (Cu6-PLGA analyzed under ApoTome fluorescence microscopy. The observations revealed that 50 nm Cu6-PLGA penetrated into hyphae, as early 10 min after administration. Pterostilbene, natural compound, fluopyram, synthetic antifungal, entrapped NPs, added mycelium, their antifungal activity was tested. results compounds exhibited higher against cinerea. These lay foundations strategy plant pest management.

Язык: Английский

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SlVQ15 interacts with jasmonate-ZIM domain proteins and SlWRKY31 to regulate defense response in tomato

PLANT PHYSIOLOGY, Год журнала: 2022, Номер 190(1), С. 828 - 842

Опубликована: Июнь 11, 2022

Abstract Botrytis cinerea is one of the most widely distributed and harmful pathogens worldwide. Both phytohormone jasmonate (JA) VQ motif-containing proteins play crucial roles in plant resistance to B. cinerea. However, their crosstalk unclear, especially tomato (Solanum lycopersicum). In this study, we found that VQ15 was highly induced upon infection localized nucleus. Silencing SlVQ15 using virus-induced gene silencing reduced Overexpression enhanced cinerea, while disruption clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein9 (Cas9) technology increased susceptibility Furthermore, formed homodimers. Additionally, interacted with JA-ZIM domain proteins, repressors JA signaling pathway, SlWRKY31. SlJAZ11 interfered interaction between SlWRKY31 repressed SlVQ15-increased transcriptional activation activity synergistically regulated as sensitivity slwrky31 Taken together, our findings showed SlJAZ-interacting protein physically interacts cooperatively control JA-mediated defense against

Язык: Английский

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From Genes to Molecules, Secondary Metabolism in Botrytis cinerea: New Insights into Anamorphic and Teleomorphic Stages

Plants, Год журнала: 2023, Номер 12(3), С. 553 - 553

Опубликована: Янв. 26, 2023

The ascomycete Botrytis cinerea Pers. Fr., classified within the family Sclerotiniaceae, is agent that causes grey mould disease which infects at least 1400 plant species, including crops of economic importance such as grapes and strawberries. life cycle B. consists two phases: asexual (anamorph, Fr.) sexual (teleomorph, Botryotinia fuckeliana (de Bary) Wetzel). During XVI International Symposium dedicated to fungus, was held in Bari June 2013, scientific community unanimously decided assign most widely used name form, Botrytis, this genus fungi. However, literature, we continue find articles referring both morphic stages. In review, take stock genes metabolites reported for forms between January 2015 October 2022.

Язык: Английский

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Genetic and molecular landscapes of the generalist phytopathogen Botrytis cinerea

Molecular Plant Pathology, Год журнала: 2023, Номер 25(1)

Опубликована: Дек. 1, 2023

Abstract Botrytis cinerea Pers. Fr. (teleomorph: Botryotinia fuckeliana ) is a necrotrophic fungal pathogen that attacks wide range of plants. This updated profile explores the extensive genetic diversity B. , highlights progress in genome sequencing, and provides current knowledge molecular mechanisms employed by fungus to attack its hosts. In addition, we also discuss recent innovative strategies combat . Taxonomy Kingdom: Fungi, phylum: Ascomycota, subphylum: Pezizomycotina, class: Leotiomycetes, order: Helotiales, family: Sclerotiniaceae, genus: species: Host infects almost all plant groups (angiosperms, gymnosperms, pteridophytes, bryophytes). To date, 1606 species have been identified as hosts Genetic polyphagous necrotroph has at population levels shaped climate, geography, host variation. Pathogenicity architecture virulence specificity polygenic using multiple weapons target hosts, including secretory proteins, complex signal transduction pathways, metabolites, mobile small RNA. Disease control Efforts being high‐diversity generalist pathogen, are complicated. However, integrated disease management combine cultural practices, chemical biological controls, use appropriate crop varieties will lessen yield losses. Recently, studies conducted worldwide explored potential RNA an efficient environmentally friendly approach for combating grey mould. additional research necessary, especially on risk assessment regulatory frameworks, fully harness this technology.

Язык: Английский

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Challenges and Opportunities Arising from Host–Botrytis cinerea Interactions to Outline Novel and Sustainable Control Strategies: The Key Role of RNA Interference

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(12), С. 6798 - 6798

Опубликована: Июнь 20, 2024

The necrotrophic plant pathogenic fungus Botrytis cinerea (Pers., 1794), the causative agent of gray mold disease, causes significant losses in agricultural production. Control this fungal pathogen is quite difficult due to its wide host range and environmental persistence. Currently, management disease still mainly based on chemicals, which can have harmful effects not only environment human health but also because they favor development strains resistant fungicides. flexibility plasticity B. challenging defense mechanisms ability evolve strategies escape chemicals require new control for successful management. In review, some aspects host-pathogen interactions from novel sustainable could be developed (e.g., signaling pathways, molecules involved immune mechanisms, hormones, post-transcriptional gene silencing) were analyzed. New biotechnological tools use RNA interference (RNAi) are emerging crop protection scenario as versatile, sustainable, effective, environmentally friendly alternatives chemicals. RNAi-based fungicides expected approved soon, although will face several challenges before reaching market.

Язык: Английский

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Total Synthesis of Fusaramin, Enabling Stereochemical Elucidation, Structure–Activity Relationship, and Uncovering the Hidden Antimicrobial Activity against Plant Pathogenic Fungi

Organic Letters, Год журнала: 2024, Номер 26(3), С. 597 - 601

Опубликована: Янв. 10, 2024

Fusaramin (1) was isolated as a mitochondrial inhibitor. However, the fungal producer stops producing 1, which necessitates us to supply 1 by total synthesis. We proposed complete stereochemical structure based on biosynthetic pathway of sambutoxin. have established concise and robust synthesis enabling determine elucidate structure–activity relationship, uncover hidden antiplant pathogenic activity.

Язык: Английский

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