Frontiers in Plant Science,
Год журнала:
2022,
Номер
13
Опубликована: Март 28, 2022
Plants
have
amazing
regenerative
properties
with
single
somatic
cells,
or
groups
of
cells
able
to
give
rise
fully
formed
plants.
One
means
regeneration
is
embryogenesis,
by
which
an
embryonic
structure
that
"converts"
into
a
plantlet.
Somatic
embryogenesis
has
been
used
as
model
for
zygotic
processes
are
buried
within
layers
maternal
tissues.
Understanding
mechanisms
embryo
induction
and
development
important
more
accessible
seed
development.
We
rely
on
not
only
most
our
caloric
intake,
but
also
delivery
system
engineered
crops
meet
agricultural
challenges.
Regeneration
transformed
needed
this
applied
work
well
basic
research
understand
gene
function.
Here
we
focus
MADS-domain
transcription
factor,
AGAMOUS-Like15
(AGL15)
shows
positive
correlation
between
accumulation
levels
capacity
embryogenesis.
relate
AGL15
function
other
factors,
hormones,
epigenetic
modifiers
involved
in
Frontiers in Plant Science,
Год журнала:
2022,
Номер
13
Опубликована: Июнь 30, 2022
Plant
regeneration
occurs
when
plants
repair
or
replace
damaged
structures
based
on
the
totipotency
and
pluripotency
of
their
cells.
Tissue
culture
is
one
most
widely
used
regenerative
technologies.
Recently,
a
series
breakthroughs
were
made
in
study
plant
regeneration.
This
review
summarizes
two
pathways
tissue
culture:
somatic
embryogenesis
de
novo
organogenesis.
Furthermore,
we
environmental
factors
influencing
from
explant
sources,
basal
medium,
growth
regulators,
light/dark
treatment.
Additionally,
analyse
molecular
mechanisms
underlying
pathways.
knowledge
will
promote
an
understanding
fundamental
principles
precursor
cells
lay
solid
foundation
for
applying
micropropagation
genetic
modification.
Horticulturae,
Год журнала:
2022,
Номер
8(8), С. 677 - 677
Опубликована: Июль 25, 2022
The
production
of
micropropagated
plants
in
plant-tissue-culture
laboratories
and
nurseries
is
the
most
important
method
for
propagation
many
economic
plants.
Micropropagation
based
on
tissue-culture
technology
involves
large-scale
propagation,
as
it
allows
multiplication
a
huge
number
true-to-type
propagules
very
short
time
limited
space,
well
all
year
round,
regardless
climate.
However,
applying
techniques
commercial
may
face
lot
obstacles
or
troubles
that
could
result
from
technical,
biological,
physiological,
and/or
genetical
reasons,
due
to
overproduction
lack
facilities
professional
technicians,
shown
current
study.
Moreover,
several
disorders
abnormalities
are
discussed
present
review.
This
study
aims
show
serious
problems
plant
micropropagation,
their
solutions
both
scientific
technical
sides.
review,
first
report,
includes
different
challenges
micropropagation
(i.e.,
contamination,
delay
subculture,
burned
plantlets,
browning,
vitro
rooting
difficulty,
somaclonal
variations,
hyperhydricity,
shoot
tip
necrosis,
albino
recalcitrance,
abnormalities,
habituation)
one
paper.
Most
these
related
they
be
avoided
by
following
protocol
suitable
each
species.
others
dominant
laboratories,
which
often
incomplete,
poor
infrastructure
scarce
funds.
Plants,
Год журнала:
2021,
Номер
10(1), С. 185 - 185
Опубликована: Янв. 19, 2021
The
recent
legalization
of
Cannabis
sativa
L.
in
many
regions
has
revealed
a
need
for
effective
propagation
and
biotechnologies
the
species.
Micropropagation
affords
researchers
producers
methods
to
rapidly
propagate
insect-/disease-/virus-free
clonal
plants
store
germplasm
forms
basis
other
biotechnologies.
Despite
this
need,
research
area
is
limited
due
long
history
prohibitions
restrictions.
Existing
literature
multiple
limitations:
publications
use
hemp
as
proxy
drug-type
when
it
well
established
that
there
significant
genotype
specificity;
studies
using
cultivars
are
predominantly
optimized
single
cultivar;
most
protocols
have
not
been
replicated
by
independent
groups,
some
attempts
demonstrate
lack
reproducibility
across
genotypes.
Due
culture
decline
problems,
multiplication
phase
micropropagation
(Stage
2)
fully
developed
reports.
This
review
will
provide
brief
background
on
botany
comprehensive
critical
summary
tissue
culture.
Special
attention
be
paid
current
challenges
faced
researchers,
limitations
existing
studies,
developments
future
directions
technologies.
Frontiers in Microbiology,
Год журнала:
2021,
Номер
12
Опубликована: Март 29, 2021
Plant
growth
promoting
microbes
(PGPMs)
play
major
roles
in
diverse
ecosystems,
including
atmospheric
nitrogen
fixation,
water
uptake,
solubilization,
and
transport
of
minerals
from
the
soil
to
plant.
Different
PGPMs
are
proposed
as
biofertilizers,
biostimulants,
and/or
biocontrol
agents
improve
plant
productivity
thereby
contribute
agricultural
sustainability
food
security.
However,
little
information
exists
regarding
use
micropropagation
such
vitro
tissue
culture.
This
review
presents
an
overview
importance
their
potential
application
micropropagation.
Our
analysis,
based
on
published
articles,
reveals
that
process
classical
culture
techniques,
under
strictly
aseptic
conditions,
deserves
be
reviewed
allow
vitroplants
benefit
positive
effect
PGPMs.
Furthermore,
exploiting
benefits
will
lead
lessen
cost
production
during
make
technique
more
efficient.
The
last
part
indicate
where
research
is
needed
future.
International Journal of Molecular Sciences,
Год журнала:
2021,
Номер
22(11), С. 5671 - 5671
Опубликована: Май 26, 2021
For
a
long
time,
Cannabis
sativa
has
been
used
for
therapeutic
and
industrial
purposes.
Due
to
its
increasing
demand
in
medicine,
recreation,
industry,
there
is
dire
need
apply
new
biotechnological
tools
introduce
genotypes
with
desirable
traits
enhanced
secondary
metabolite
production.
Micropropagation,
conservation,
cell
suspension
culture,
hairy
root
polyploidy
manipulation,
Agrobacterium-mediated
gene
transformation
have
studied
cannabis.
However,
some
obstacles
such
as
the
low
rate
of
transgenic
plant
regeneration
efficiency
production
culture
restricted
application
these
approaches
In
current
review,
vitro
genetic
engineering
methods
cannabis
along
other
promising
techniques
morphogenic
genes,
computational
approaches,
clustered
regularly
interspaced
short
palindromic
repeats
(CRISPR),
CRISPR/Cas9-equipped
genome
editing,
that
can
help
improve
regeneration,
well
enhance
production,
highlighted
discussed.
Plants,
Год журнала:
2020,
Номер
9(10), С. 1261 - 1261
Опубликована: Сен. 24, 2020
Plant
regeneration
is
essential
for
survival
upon
wounding
and
is,
hence,
considered
to
be
a
strong
natural
selective
trait.
The
capacity
of
plant
tissues
regenerate
in
vitro,
however,
varies
substantially
between
within
species
depends
on
the
applied
incubation
conditions.
Insight
into
genetic
factors
underlying
this
variation
may
help
improve
numerous
biotechnological
applications
that
exploit
vitro
regeneration.
Here,
we
review
state
art
molecular
framework
de
novo
shoot
organogenesis
from
root
explants
Arabidopsis,
which
complex
process
controlled
by
multiple
quantitative
trait
loci
various
effect
sizes.
Two
types
are
distinguished
contribute
regenerative
variation:
master
regulators
conserved
all
experimental
systems
(e.g.,
WUSCHEL
related
homeobox
genes)
conditional
whose
relative
role
explant
settings.
We
further
elaborate
epigenetic
protocol
variables
likely
differential
responsivity
conclude
occurs
at
intersection
(epi)
genetics,
endogenous
hormone
levels,
environmental
influences.
International Journal of Molecular Sciences,
Год журнала:
2021,
Номер
22(11), С. 6157 - 6157
Опубликована: Июнь 7, 2021
Numerous
environmental
and
endogenous
factors
affect
the
level
of
genetic
diversity
in
natural
populations.
Genetic
variability
is
cornerstone
evolution
adaptation
species.
However,
currently,
more
plant
species
local
varieties
(landraces)
are
on
brink
extinction
due
to
anthropopression
climate
change.
Their
preservation
imperative
for
sake
future
breeding
programs.
Gene
banks
have
been
created
worldwide
conserve
different
cultural
economic
importance.
Many
them
apply
cryopreservation,
a
conservation
method
which
ultra-low
temperatures
(-135
°C
-196
°C)
used
long-term
storage
tissue
samples,
with
little
risk
variation
occurrence.
Cells
can
be
successfully
cryopreserved
liquid
nitrogen
(LN)
when
adverse
effect
ice
crystal
formation
growth
mitigated
by
removal
water
so-called
biological
glass
(vitrification).
This
state
achieved
several
ways.
The
involvement
key
cold-regulated
genes
proteins
acquisition
cold
tolerance
tissues
may
additionally
improve
survival
LN-stored
explants.
present
review
explains
importance
cryostorage
agronomy
presents
an
overview
recent
works
accomplished
this
strategy.
most
widely
cryopreservation
techniques,
classic
modern
cryoprotective
agents,
some
protocols
applied
crops
considered
understand
parameters
provide
establishment
high
quality
broadly
applicable
cryopreservation.
Attention
also
focused
issues
integrity
functional
genomics
cryobiology.
