Biomass Conversion and Biorefinery,
Год журнала:
2022,
Номер
14(22), С. 28253 - 28270
Опубликована: Ноя. 11, 2022
Abstract
The
goal
of
the
current
work
was
to
investigate
antibacterial,
antibiofilm,
anticancer,
and
antioxidant
opportunities
silver
gold
nanoparticles
(AgNPs
AuNPs)
synthesized
utilizing
a
new
fungus
strain
called
Fusarium
pseudonygamai
TB-13c.
With
aid
UV,
HR-TEM,
FTIR,
SEM,
XRD,
NPs’
creation
examined.
For
AgNPs
AuNPs,
mycosynthesized
highest
peak
plasmon
band
seen
at
around
420
540
nm,
respectively.
ranged
in
size
from
5
20
whereas
AuNPs
8
60
nm.
were
spherical
form.
AgNPs,
angles
38.42°,
44.56°,
64.66°,
77.75°;
for
38.56°,
44.74°,
64.87°,
77.85°.
antibacterial
efficiency
evaluated
against
Klebsiella
pneumoniae
,
Pseudomonas
aeruginosa
methicillin-sensitive
Staphylococcus
aureus
(MSSA),
methicillin-resistant
(MRSA).
In
particular,
outperformed
their
ability
combat
pathogenic
microorganisms.
Furthermore,
antibiofilm
study
that
shown
had
activity
more
than
AgNPs.
Interestingly,
applying
DPPH
procedure
these
noble
metallic
NPs
activity,
which
IC
50
38.2
μg/
mL
180
AuNPs.
modification
cells
evident
cytotoxicity
evaluation
findings
as
change
usual
shape,
partially
or
completely
loss
monolayer,
granulation,
shrinkage,
cell
rounding
with
normal
vero
695.34
μg/mL
631.66
respectively,
whilst
cancer
(Mcf7)
204.07
206.95
combined
results
show
there
is
clear
flexible
biological
use
less
toxic
chemotherapeutic
agents.
Archives of Microbiology,
Год журнала:
2023,
Номер
205(4)
Опубликована: Март 22, 2023
The
utilization
of
living
organisms
for
the
creation
inorganic
nanoscale
particles
is
a
potential
new
development
in
realm
biotechnology.
An
essential
milestone
nanotechnology
process
creating
dependable
and
environmentally
acceptable
metallic
nanoparticles.
Due
to
its
increasing
popularity
ease,
use
ambient
biological
resources
quickly
becoming
more
significant
this
field
study.
phrase
"green
nanotechnology"
has
gained
lot
attention
refers
variety
procedures
that
eliminate
or
do
away
with
hazardous
compounds
repair
environment.
Green
nanomaterials
can
be
used
biotechnological
sectors
such
as
medicine
biology,
well
food
textile
industries,
wastewater
treatment
agriculture
field.
construction
an
updated
level
knowledge
study
systems
might
support
revolutionize
nanoparticles
(NPs)
are
presented
article.
Applied Biochemistry and Biotechnology,
Год журнала:
2022,
Номер
195(1), С. 467 - 485
Опубликована: Сен. 10, 2022
Abstract
Mycosynthesis
of
nanoparticle
(NP)
production
is
a
potential
ecofriendly
technology
for
large
scale
production.
In
the
present
study,
copper
oxide
nanoparticles
(CuONPs)
have
been
synthesized
from
live
cell
filtrate
fungus
Penicillium
chrysogenum
.
The
created
CuONPs
were
characterized
via
several
techniques,
namely
Fourier
transform
infrared
(FTIR)
spectroscopy,
X-ray
diffraction
(XRD),
transmission
electron
microscope
(TEM),
scanning
(SEM),
and
energy-dispersive
spectroscopy
(EDX).
Furthermore,
biosynthesized
performed
against
biofilm
forming
Klebsiella
oxytoca
ATCC
51,983,
Escherichia
coli
35,218,
Staphylococcus
aureus
25,923,
Bacillus
cereus
11,778.
anti-bacterial
activity
result
was
shown
with
zone
inhibition
determined
to
be
14
±
0.31
mm,
16
0.53
11
0.57
10
mm
respectively.
more
susceptible
minimal
inhibitory
concentration
(MIC)
values
6.25
3.12
µg/mL,
respectively,
while
,
MIC
value
12.5
25
μg/mL,
minimum
(MBIC)
evident,
that
excellent
anti-biofilm
at
sub-MIC
levels
reducing
formation
by
49%
59%
results
indicated
MBIC
on
higher
than
200
μg/mL
256
suggesting
these
could
not
inhibit
mature
formatted
in
vitro.
Overall,
all
clearly
confirmed
ability
prepared
offer
smart
approach
biomedical
therapy
resistant
microorganisms
because
its
promoted
antimicrobial
action,
but
only
specified
purposes.
Applied Biochemistry and Biotechnology,
Год журнала:
2022,
Номер
195(2), С. 1158 - 1183
Опубликована: Ноя. 7, 2022
The
World
Health
Organization
(WHO)
reports
that
the
emergence
of
multidrug-resistant
and
slow
advent
novel
more
potent
antitumor
antimicrobial
chemotherapeutics
continue
to
be
highest
concern
for
human
health.
Additionally,
stability,
low
solubility,
negative
effects
existing
drugs
make
them
ineffective.
Studies
into
alternative
tactics
tackle
such
tenacious
diseases
was
sparked
by
anticancer
antibacterial.
Silver
(Ag)
gold
(Au)
nanoparticles
(NPs)
were
created
from
Trichoderma
saturnisporum,
much
productive
fungal
strain.
Functional
extracellular
enzymes
proteins
carried
out
activities
synthesis
capping
generated
nano-metals.
Characterization
done
on
obtained
Ag-NPs
Au-NPs
through
UV-vis,
FTIR,
XRD,
TEM,
SEM.
versus
methicillin-sensitive
Staphylococcus
aureus
(MSSA)
methicillin-resistant
(MRSA),
Pseudomonas
aeruginosa,
Klebsiella
pneumoniae,
antibacterial
assessed.
In
particular,
effective
against
pathogenic
bacteria
than
Au-NPs.
Furthermore,
antibiofilm
study
shown
had
activity
Ag-NPs.
Interestingly,
applying
DPPH
procedure,
these
noble
metallic
NPs
antioxidant
activity,
in
which
IC50
73.5
μg/mL
190.0
μg/mL,
respectively.
According
cytotoxicity
evaluation
results,
alteration
cells
as
loss
their
typical
shape,
partial
or
complete
monolayer,
granulation,
shrinking,
cell
rounding
with
normal
Vero
693.68
661.24
Au-NPs,
While
cancer
(Mcf7)
370.56
394.79
produced
via
green
have
potential
employed
medical
industry
beneficial
nanocompounds.
Applied Biochemistry and Biotechnology,
Год журнала:
2023,
Номер
196(1), С. 85 - 98
Опубликована: Апрель 26, 2023
Abstract
New
and
creative
methodologies
for
the
fabrication
of
silver
nanoparticles
(Ag-NPs),
which
are
exploited
in
a
wide
range
consumer
items,
significant
interest.
Hence,
this
research
emphasizes
biological
approach
Ag-NPs
through
Egyptian
henna
leaves
(
Lawsonia
inermis
Linn.)
extracts
analysis
prepared
Ag-NPs.
Plant
extract
components
were
identified
by
gas
chromatography
mass
spectrometry
(GC-mass).
The
analyses
carried
out
UV–visible
(UV–Vis),
X-ray
diffraction
(XRD),
transmission
electron
microscope
(TEM),
scanning
(SEM),
Fourier
transform
infrared
(FTIR)
analysis.
UV–Vis
reveals
that
have
maximum
peak
at
460
nm
visible
light.
Structural
characterization
recorded
peaks
corresponded
to
Bragg’s
diffractions
nano-crystal,
with
average
crystallite
sizes
varying
from
28
60
nm.
Antibacterial
activities
examined,
it
is
observed
all
microorganisms
very
sensitive
biologically
synthesized
Journal of Functional Biomaterials,
Год журнала:
2022,
Номер
13(4), С. 242 - 242
Опубликована: Ноя. 15, 2022
Currently,
nanoparticles
and
nanomaterials
are
widely
used
for
biomedical
applications.
In
the
present
study,
silver
(AgNPs)
were
successfully
biosynthesized
using
a
cell-free
extract
(CFE)
of
Bacillus
thuringiensis
MAE
6
through
green
ecofriendly
method.
The
size
AgNPs
was
32.7
nm,
their
crystalline
nature
confirmed
by
XRD,
according
to
characterization
results.
A
surface
plasmon
resonance
spectrum
obtained
at
420
nm.
Nanoparticles
further
characterized
DLS
FTIR
analyses,
which
provided
information
on
size,
stability,
functional
groups.
revealed
less
cytotoxicity
against
normal
Vero
cell
line
[IC50
=
155
μg/mL].
Moreover,
exhibited
promising
antifungal
activity
four
most
common
Aspergillus,
including
Aspergillus
niger,
A.
terreus,
flavus,
fumigatus
concentrations
500
μg/mL
where
inhibition
zones
16,
20,
26,
19
mm,
respectively.
addition,
MICs
125,
62.5,
15.62,
62.5
μg/mL,
Furthermore,
ultrastructural
study
effect
AgNPs,
wall's
integrity
homogeneity
lost;
membrane
had
separated
from
wall
intruded
into
cytoplasm.
conclusion,
CFE
B.
can
be
as
agent
species
causing
Aspergillosis.
Bioengineering,
Год журнала:
2022,
Номер
9(9), С. 452 - 452
Опубликована: Сен. 8, 2022
In
the
study
reported
in
this
manuscript,
silver/iron
oxide
nanocomposites
(Ag/Fe2O3)
were
phytosynthesized
using
extract
of
Buddleja
lindleyana
via
a
green,
economical
and
eco-friendly
strategy.
The
biosynthesized
Ag/Fe2O3
characterized
UV-Vis
spectrophotometry,
FTIR,
XRD,
TEM,
DLS
SEM-EDX
analyses.
particulates
showed
triangular
spherical
morphology
having
sizes
between
25
174
nm.
FTIR
studies
on
nanoparticles
functional
groups
corresponding
to
organic
metabolites,
which
reduce
stabilize
nanocomposite.
antimicrobial
efficacy
against
bacterial
pathogens
was
assessed.
addition,
exhibited
broad
spectrum
activities
B.
subtilis,
S.
aureus,
E.
coli,
P.
aeruginosa
with
inhibition
zones
23.4
±
0.75,
22.3
0.57,
20.8
1.6,
19.5
0.5
mm,
respectively.
composites
obtained
promising
antibacterial
action
human
(S.
subtilis
aeruginosa),
making
them
candidates
for
medical
applications.
