Journal of Agricultural and Food Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 7, 2025
l-Lactic
acid
is
a
three-carbon
monocarboxylic
that
has
extensive
applications.
However,
the
bioproduction
of
l-lactic
requires
addition
neutralizers,
which
significantly
increases
production
costs
and
can
cause
environmental
pollution.
To
address
this,
Saccharomyces
cerevisiae
mutant,
TMG2,
tolerate
lactic
environment
(pH
2.60),
was
obtained
through
adaptive
laboratory
evolution.
Subsequently,
"push-pull-restrain"
strategy
used
to
improve
production,
resulting
in
46.8
g/L
acid.
Finally,
by
overexpressing
transport
protein
pPfFNT
improving
NADH
acetyl-CoA
supply,
titer
strain
TMG27
improved
33.8%
62.6
g/L.
Without
reached
76.2
(the
fermentation
pH
2.90)
with
productivity
2.1
g/(L
h)
5-L
bioreactor,
representing
highest
ever
reported.
Collectively,
these
results
lay
foundation
for
environmentally
friendly
Microorganisms,
Год журнала:
2023,
Номер
11(6), С. 1408 - 1408
Опубликована: Май 26, 2023
With
the
expansion
of
green
products
market
and
worldwide
policies
strategies
directed
toward
a
revolution
ecological
transition,
demand
for
innovative
approaches
is
always
on
rise.
Among
sustainable
agricultural
approaches,
microbial-based
are
emerging
over
time
as
effective
feasible
alternatives
to
agrochemicals.
However,
production,
formulation,
commercialization
some
can
be
challenging.
main
challenges
industrial
production
processes
that
ensure
quality
product
its
cost
market.
In
context
circular
economy,
solid-state
fermentation
(SSF)
might
represent
smart
approach
obtaining
valuable
from
waste
by-products.
SSF
enables
growth
various
microorganisms
solid
surfaces
in
absence
or
near
free-flowing
water.
It
practical
method
used
food,
pharmaceutical,
energy,
chemical
industries.
Nevertheless,
application
this
technology
formulations
useful
agriculture
still
limited.
This
review
summarizes
literature
dealing
with
applications
future
perspective
use
agriculture.
The
survey
showed
good
potential
produce
biostimulants
biopesticides
Fermentation,
Год журнала:
2023,
Номер
9(11), С. 990 - 990
Опубликована: Ноя. 20, 2023
Lignocellulose
consists
of
cellulose,
hemicellulose,
and
lignin
is
a
sustainable
feedstock
for
biorefinery
to
generate
marketable
biomaterials
like
biofuels
platform
chemicals.
Enormous
tons
lignocellulose
are
obtained
from
agricultural
waste,
but
few
utilized
due
lack
awareness
the
biotechnological
importance
lignocellulose.
Underutilizing
could
also
be
linked
incomplete
use
cellulose
hemicellulose
in
biotransformation
into
new
products.
Utilizing
producing
value-added
products
alleviates
waste
disposal
management
challenges.
It
reduces
emission
toxic
substances
environment,
which
promotes
development
goal
contributes
circular
economy
economic
growth.
This
review
broadly
focused
on
production
high-value
The
aspects
that
were
discussed
included:
(i)
sources
lignocellulosic
biomass;
(ii)
conversion
biomass
products;
(iii)
various
bio-based
Additionally,
several
challenges
upcycling
alleviation
strategies
discussed.
suggested
prospects
using
replace
polystyrene
packaging
with
lignin-based
products,
crafts
interior
decorations
lignin,
nanolignin
environmental
biosensors
biomimetic
sensors,
processing
addition
nutritional
supplements
meet
dietary
requirements
animal
feeding.
Metabolic Engineering,
Год журнала:
2024,
Номер
84, С. 23 - 33
Опубликована: Май 23, 2024
Metabolic
engineering
for
high
productivity
and
increased
robustness
is
needed
to
enable
sustainable
biomanufacturing
of
lactic
acid
from
lignocellulosic
biomass.
Lactic
an
important
commodity
chemical
used
instance
as
a
monomer
production
polylactic
acid,
biodegradable
polymer.
Here,
rational
model-based
optimization
was
engineer
diploid,
xylose
fermenting
Saccharomyces
cerevisiae
strain
produce
L-lactic
acid.
The
metabolic
flux
steered
towards
through
the
introduction
multiple
lactate
dehydrogenase
encoding
genes
while
deleting
ERF2,
GPD1,
CYB2.
A
93
g/L
with
yield
0.84
g/g
achieved
using
carbon
source.
To
increase
utilization
reduce
acetic
synthesis,
PHO13
ALD6
were
also
deleted
strain.
Finally,
CDC19
pyruvate
kinase
overexpressed,
resulting
in
0.75
g
/g
sugars
consumed,
when
substrate
synthetic
hydrolysate
medium,
containing
hexoses,
pentoses
inhibitors
such
acetate
furfural.
Notably,
modeling
provided
leads
understanding
influence
oxygen
production.
High
xylose,
at
oxygen-limitation
could
be
explained
by
reduced
oxidative
phosphorylation
pathway.
On
contrast,
higher
levels
beneficial
likely
ATP
concentrations
are
tolerating
therein.
work
highlights
potential
S.
industrial
Biotechnology for Biofuels and Bioproducts,
Год журнала:
2024,
Номер
17(1)
Опубликована: Май 6, 2024
Abstract
Background
Lignocellulosic
biomass
as
feedstock
has
a
huge
potential
for
biochemical
production.
Still,
efficient
utilization
of
hydrolysates
derived
from
lignocellulose
is
challenged
by
their
complex
and
heterogeneous
composition
the
presence
inhibitory
compounds,
such
furan
aldehydes.
Using
microbial
consortia
where
two
specialized
microbes
complement
each
other
could
serve
approach
to
improve
efficiency
lignocellulosic
upgrading.
Results
This
study
describes
simultaneous
inhibitor
detoxification
production
lactic
acid
wax
esters
synthetic
hydrolysate
defined
coculture
engineered
Saccharomyces
cerevisiae
Acinetobacter
baylyi
ADP1.
A.
ADP1
showed
bioconversion
aldehydes
present
in
hydrolysate,
namely
furfural
5-hydroxymethylfurfural,
did
not
compete
substrates
with
S.
,
highlighting
its
partner.
Furthermore,
remaining
carbon
sources
byproducts
were
directed
ester
The
productivity
was
improved
approximately
1.5-fold
(to
0.41
±
0.08
g/L/h)
ADP1,
compared
monoculture
.
Conclusion
yeast
bacterium
shown
consumption
hydrolysate.
high
capacity
ability
produce
high-value
products
demonstrates
strain
be
candidate
increase
economics
fermentations.
