Integrating in vitro and in silico techniques for evaluating the antioxidant activity of Chrysanthemum indicum L
Pharmacia,
Год журнала:
2025,
Номер
72, С. 1 - 14
Опубликована: Янв. 9, 2025
The
prevalence
of
degenerative
diseases
has
been
escalating
due
to
the
mounting
presence
oxidative
stress
conditions,
characterized
by
elevated
levels
reactive
oxygen
species
(ROS).
Oxidative
triggers
genetic
alterations,
structural
modifications,
and
progression
various
disorders,
including
cancer,
diabetes,
Parkinson’s
disease,
Alzheimer’s
rheumatoid
arthritis,
osteoporosis.
Consequently,
modulation
redox
homeostasis
attenuation
through
activity
antioxidant
compounds
play
crucial
roles.
Chrysanthemum
indicum
L.,
an
herb
with
rich
historical
usage
for
alleviating
diverse
discomforts
stress-related
ailments,
possesses
potential
properties.
Nevertheless,
precise
mechanism
underlying
its
efficacy
remains
poorly
understood.
In
this
study,
chrysanthemum
flower
ethanol
extract
was
investigated
assessing
ability
inhibit
2,2-dyphenyl-1-picrylhydrazyl
(DPPH)
radicals.
Additionally,
detected
present
in
flowers
were
computationally
docked
key
macromolecular
receptors
involved
(ROS)
production,
namely
Cytochrome
P450
(CP450)
NADPH
oxidase
(NO).
findings
revealed
that
ethanolic
displayed
inhibitory
against
radicals
at
a
concentration
1.35
µg/mL,
which
is
comparable
vitamin
C
(0.7
µg/mL).
Moreover,
bioactive
flowers,
3-hydroxyspirost-8-en-11-one,
ingenol
3-hexadecanoate,
Ergosta-5,22-dien-3-ol
acetate,
subjected
docking
simulations
CP450
NO
receptors.
binding-free
energies
(BFEs)
acquired
compound
3-Hydroxyspirost-8-en-11-one
determined
be
-12.7
kcal/mol
-12.4
receptors,
respectively.
Similarly,
Ingenol
3-hexadecanoate
exhibited
BFE
values
-9.5
-10.7
kcal/mol,
while
acetate
BFEs
-8.9
-8.1
contrast,
control
substances
5-fluorouracil
(FLU)
dextromethorphan
(DEX)
demonstrated
-4.9
-6.5
respectively,
each
receptor.
Collectively,
our
suggest
holds
promise
as
natural
source
preventing
diseases,
cancer
diabetes.
Язык: Английский
Phytochemical Analysis and Compound Profiling of Boerhavia elegans Stem Extract Using GC–MS
Analytical Science Advances,
Год журнала:
2025,
Номер
6(1)
Опубликована: Март 23, 2025
ABSTRACT
Natural
products
are
essential
in
drug
discovery
and
have
diverse
applications
biotechnology.
Naturally
derived
compounds
widely
recognized
as
lead
candidates
conventional
development.
Molecular
docking
has
become
a
valuable
tool
for
predicting
interactions
between
small
molecules
targets,
assisting
medicinal
chemists
designing
with
potential
pharmacological
effects.
This
study
aimed
to
investigate
the
phytoconstituents
of
methanol
extract
Boerhavia
elegans
stem
through
gas
chromatography–mass
spectrometry
(GC–MS)
analysis
conduct
molecular
studies
active
components
against
BCL2
receptor
protein.
Liquid–liquid
extractions
were
performed
using
n
‐hexane,
chloroform
butanol,
followed
by
GC–MS
extracts.
conducted
on
identified
22
extracts,
prominent
compounds,
including
hexadecenoic
acid,
octadec‐9‐enoic
various
benzene
dicarboxylic
acid
esters,
hexadecanoic
derivatives,
13‐docosenamide
stigmasterol.
revealed
that
γ‐sitosterol,
α‐sitosterol
campesterol
exhibited
lowest
binding
scores,
indicating
high
affinity.
These
findings
support
traditional
use
B.
treating
ailments,
results
provide
insights
into
mechanisms
action
compounds.
Язык: Английский
Nanomaterials As Sorbents in Solid-Phase Extraction for Bioactive Compounds
Separation and Purification Reviews,
Год журнала:
2024,
Номер
unknown, С. 1 - 21
Опубликована: Июнь 5, 2024
Nanomaterials
have
emerged
as
excellent
candidates
sorbents
for
solid
phase
extraction
(SPE)
techniques
owing
to
their
outstanding
properties
including
large
surface
area,
high
chemical/mechanical
stability
and
specific
affinity
toward
target
analytes.
Bioactive
compounds
occur
in
small
quantities
food
biological
matrices,
are
extensively
investigated
having
beneficial
effects
human
health,
such
antioxidant,
antimicrobial,
anti-inflammatory,
antitumor
activities,
mention
but
a
few.
metallic
nanoparticles,
magnetic
metal
oxides,
polymer-based
nanocomposites,
carbon-based
nanomaterials
been
integrated
different
sample
preparation
the
efficient
preconcentration
of
biocompounds.
This
review
focuses
on
analysis
bioactive
matrices
using
SPE
techniques.
A
general
vision
currently
being
developed
this
field
is
provided,
most
representative
examples
described.
Their
efficiency
sensitivity
comparatively
discussed.
Further,
challenges
future
prospects
highlighted.
Язык: Английский
Identification and Assessment of Therapeutic Phytoconstituents of Catharanthus roseus through GC-MS Analysis
Separations,
Год журнала:
2023,
Номер
10(6), С. 340 - 340
Опубликована: Июнь 1, 2023
The
leaves
of
Catharanthus
roseus
(L.)
G.
Don
contain
a
large
number
diverse
secondary
metabolites,
making
them
comparably
complex.
genus
has
received
increased
interest
from
scientists
in
recent
years
due
to
its
extensive
applications
several
domains,
including
the
pharmaceutical
sector,
where
precise
characterization
characteristics
is
required.
An
effective
inquiry
technique
needed
for
chemo-profiling
identify
metabolites
plant
samples.
main
goal
this
research
provide
supplementary
data
on
chemical
composition
twenty-five
different
accessions
C.
through
application
gas
chromatography-mass
spectrometry
(GC-MS).
study’s
findings
reveal
existence
vast
phytochemicals,
allowing
comparison
accessions.
Furthermore,
meticulous
statistical
analysis
using
principal
components
(PCA)
and
heatmap,
hierarchical
cluster
(HCA)
may
aid
providing
more
relevant
information
possible
investigation
their
further
scientific
studies.
Язык: Английский
The Cytotoxic and Anti-tumor Potential of Methanolic Extracts of Indian Marine Isolates in HCT116 Colorectal Cancer Cells
A. K. M. Shahjahan,
Sruthi Sekar,
Kumaran Kasinathan
и другие.
Anti-Cancer Agents in Medicinal Chemistry,
Год журнала:
2023,
Номер
23(17), С. 1974 - 1981
Опубликована: Авг. 11, 2023
The
marine
environment
is
a
rich
source
of
biodiversity,
with
several
its
inhabitants
producing
unique
and
physiologically
active
substances.
use
bacterial-derived
chemicals
over
traditional
pharmaceuticals
gaining
traction
due
to
their
larger
variety
targets
modes
action.
To
circumvent
the
drawbacks
current
therapy
options,
researchers
have
looked
microbes
for
novel
effective
anti-cancer
compounds.
In
this
study,
we
examine
one
India's
least-examined
coastal
areas
in
search
bacterial
sources
chemicals.Soil
sediments
from
Indian
south
coast
region
were
collected
isolated
using
standard
methods.
microorganisms
identified
16s
rRNA
sequencing,
cytotoxic
extracts
further
examined
GC-MS.
MTT,
clonogenic,
spheroid
tests
assessed
extract's
cytotoxicity
anti-tumor
efficacy.Our
results
indicated
that
isolates
potent
activity
Bacillus
drentensis
haikouensis
had
10
12
other
bioactive
an
IC50
30.08
109.4
μg/ml
HCT116
cell
line,
respectively,
strongly
inhibited
colony
formation.
cycle
analysis
extract
induced
death
as
by
subG0
peak.
We
also
showed
these
methanolic
toxicity
3D
model
indicating
strong
activity.
Furthermore,
performed
molecular
docking
compounds
present
VEGFR
nucleolin
found
ergostane
favorable
binding
energy
only
VEGFR.The
indicate
ME
B.
contains
exhibit
colorectal
cancer
cells.
Язык: Английский
Green synthesis of silver nanoparticles by Smyrnium cordifolium plant and its application for colorimetric detection of ammonia
Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Окт. 15, 2024
The
need
to
identify
ammonia
is
necessary
because
of
its
harmful
effects
on
the
environment
and
humans.
In
this
study,
a
colorimetric
method
was
also
developed
for
detection
using
silver
nanoparticles
(AgNPs)
synthesized
with
green
approach.
Biosynthesis
AgNPs
performed
by
nitrate
as
precursor
Smyrnium
cordifolium
extract
reducing
stabilizing
agent.
Plant
studied
FTIR
LC/Mass
techniques.
optimization
effective
parameters
carried
out
central
composite
design
according
concentration,
plant
volume,
pH,
temperature.
Biosynthetic
nano-silver
characterized
XRD,
EDS/EDX,
FE-SEM,
FTIR,
TGA,
DLS
methods.
validated
detection.
were
increased
in
20
mM
AgNO
Язык: Английский
Local Knowledge on Wild Edible and Economically Important Pteridophytes of India: A Review
Human Ecology,
Год журнала:
2023,
Номер
51(5), С. 937 - 977
Опубликована: Окт. 1, 2023
Язык: Английский
Unveiling Nature’s Treasures: Exploring Bioactive Compounds from Algae for Extraction, Refinement, and Diverse Applications
Опубликована: Янв. 1, 2024
Exploring
bioactive
compounds
from
algae
has
emerged
as
a
captivating
research
focus
in
recent
years.
Studies
have
demonstrated
that
harbor
reservoir
of
intrinsic
natural
substances
encompassing
pigments,
polyphenols,
terpenes,
polysaccharides,
vitamins,
and
other
entities,
each
bestowing
distinctive
physiological
properties
multiple
applications.
Pigments,
recognized
for
their
antioxidative
potency,
confer
cellular
protection
against
oxidative
stress
inflammation,
showcasing
potential
therapeutic
agents.
Compounds
like
polyphenols
flavonoids
gained
scientific
attention
due
to
contributions
treating
cardiovascular
health,
cognitive
well-being,
major
diseases.
Advancements
extraction
methodologies,
such
supercritical
fluid
enzyme-assisted
extraction,
been
harnessed
unlock
these
algae.
Subsequent
refinement
endeavors,
chromatographic
techniques
filtration,
ensure
the
attainment
high-purity
compounds.
These
myriad
applications
nutraceuticals,
pharmaceuticals,
food
cosmetics,
biofuels,
animal
feed,
agrochemicals,
environmental
remediation.
exhibit
promote
overall
propelling
advancements
medical
with
diverse
Despite
promising
effects,
challenges
persist,
necessitating
sustainable
practices
new
methods
refining
bioactives
evaluating
In
addition
our
existing
knowledge,
we
distinctly
emphasize
latest
purification,
methodologies.
Exploration
using
eco-friendly
and/or
cost-effective
innovative
purification
strategies
versatile
holds
key
harmonious
future
where
algal
harmonize
industry,
environment.
Язык: Английский
Steroidal Alkaloids from Food Waste of Tomato Processing Inhibit Neuroblastoma Cell Viability
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(23), С. 16915 - 16915
Опубликована: Ноя. 29, 2023
Nowadays,
there
is
considerable
attention
toward
the
use
of
food
waste
from
processing
as
possible
sources
compounds
with
health
properties,
such
anticancer
activity.
An
example
tomato
processing,
which
responsible
for
generating
a
remarkable
amount
(leaves,
peel,
seeds).
Therefore,
our
goal
was
to
evaluate
potential
property
extracts,
in
particular
"Datterino"
(DT)
and
"Piccadilly"
(PT),
study
their
phytochemical
composition.
Liquid
chromatography
tandem
mass
spectrometry
(LC/MS-MS)
results
showed
that
these
extracts
are
rich
alkaloids,
flavonoids,
fatty
acids,
lipids,
terpenes.
Furthermore,
activity
evaluated
vitro
by
MTT
assay.
In
particular,
percentage
cell
viability
assessed
olfactory
ensheathing
cells
(OECs),
glial
type
system,
SH-SY5Y,
neuroblastoma
line.
All
(aqueous
ethanolic)
did
not
lead
any
significant
change
on
OECs
when
compared
control.
Instead,
SH-SY5Y
we
observed
decrease
viability,
confirming
activity;
this
more
evident
ethanolic
extracts.
conclusion,
leaves
could
be
regarded
valuable
source
bioactive
compounds,
suitable
various
applications
food,
nutraceutical,
pharmaceutical
fields.
Язык: Английский