Vaccines, Год журнала: 2024, Номер 12(8), С. 873 - 873
Опубликована: Авг. 1, 2024
mRNA vaccines are leading a medical revolution. technologies utilize the host's own cells as bio-factories to produce proteins that serve antigens. This revolutionary approach circumvents complicated processes involved in traditional vaccine production and empowers with ability respond emerging or mutated infectious diseases rapidly. Additionally, robust cellular immune response elicited by has shown significant promise cancer treatment. However, inherent instability of complexity tumor immunity have limited its broader application. Although emergence pseudouridine ionizable cationic lipid nanoparticles (LNPs) made clinical application possible, there remains substantial potential for further improvement immunogenicity delivered antigens preventive therapeutic effects technology. Here, we review latest advancements vaccines, including but not target selection delivery systems. offers multifaceted perspective on this rapidly evolving field.
Язык: Английский
Процитировано
7Enhancing TB Vaccine Efficacy: Current Progress on Vaccines, Adjuvants and Immunization Strategies
Vaccines, Год журнала: 2023, Номер 12(1), С. 38 - 38
Опубликована: Дек. 29, 2023
Tuberculosis (TB) remains a global infectious disease primarily transmitted via respiratory tract infection. Presently, vaccination stands as the primary method for TB prevention, predominantly reliant on Bacillus Calmette-Guérin (BCG) vaccine. Although it is effective in preventing disseminated diseases children, its impact adults limited. To broaden vaccine protection, efforts are underway to accelerate development of new vaccines. However, challenges arise due limited immunogenicity and safety these vaccines, necessitating adjuvants bolster their ability elicit robust immune response improved safer immunization. These function by augmenting cellular humoral immunity against
Язык: Английский
Процитировано
7Nanoparticles‐based double‐stranded
Reviews in Aquaculture, Год журнала: 2024, Номер 16(4), С. 1647 - 1673
Опубликована: Апрель 24, 2024
Abstract The review encapsulates the comprehensive exploration of RNA interference technology's application in shrimp aquaculture, covering molecular intricacies, production methods, and practical applications. emphasis is on exploring stability delivery gene‐specific double‐stranded RNA, particularly through nanoencapsulation. This because susceptibility dsRNA to degradation limited cellular penetration, emphasising need for effective mechanisms. presents an in‐depth nanoparticles encapsulation dsRNA, including virus‐like particles (VLPs), non‐viral based such as liposomes, chitosan, beta‐glucan. VLPs, derived from non‐infectious virus structures, exhibit biocompatibility natural functionality, making them suitable carriers dsRNA. chitosan its derivatives are explored their efficacy reducing viral infections shrimp. Beta‐glucan examined immunostimulant properties aquaculture. text emphasises use yeast‐based glucan encapsulating showcasing potential preventing diseases introduces cautionary considerations nanoparticle formulation, highlighting factors pH, organic solvents, metal ions, environmental ribonuclease that can impact during synthesis. In conclusion, discusses economic worthiness risk assessment technology While acknowledging successful applications other environments, it underscores regulatory approval dsRNA‐based products aquaculture industry. evaluation real‐world farms deemed necessary commercial utilisation, taking into account income, expenses, safety, considerations.
Язык: Английский
Процитировано
2Biocompatible smart micro/nanorobots for active gastrointestinal tract drug delivery
Expert Opinion on Drug Delivery, Год журнала: 2023, Номер 20(10), С. 1427 - 1441
Опубликована: Окт. 3, 2023
Oral delivery is the most commonly used route of drug administration owing to good patient compliance. However, gastrointestinal (GI) tract contains multiple physiological barriers that limit absorption efficiency conventional passive systems resulting in a low concentration reaching diseased sites. Micro/nanorobots can convert energy self-propulsive force, providing novel platform actively overcome GI for noninvasive and treatment.In this review, we first describe microenvironments different compartments tract. Afterward, applications micro/nanorobots active are highlighted discussed. Finally, summarize discuss challenges future prospects further clinical applications.Micro/nanorobots with ability autonomously propel themselves load, transport, release payloads on demand ideal carriers oral delivery. Although there many be addressed, have great potential introduce new era precision therapy.
Язык: Английский
Процитировано
4Oral immunization against foot-and-mouth disease virus using recombinant Saccharomyces cerevisiae with the improved expression of the codon-optimized VP1 fusion protein
Veterinary Microbiology, Год журнала: 2024, Номер 296, С. 110192 - 110192
Опубликована: Июль 18, 2024
VP1, a major immunogenic protein of foot-and-mouth disease virus (FMDV), facilitates viral attachment and entry into host cells. VP1 possesses critical epitope sequences responsible for inducing neutralizing antibodies but its expression using Saccharomyces cerevisiae has been hampered despite evidence that the presence does not negatively impact yeast's biology. In this study, we fused proteins to enhance S. cerevisiae. Among short P1 chimeras containing including VP3-VP1 VP2-VP1, fusion showed higher levels than VP2-VP1. We subsequently designed new proteins, which 20 amino acids N-terminal VP3 with VP1-Co1 (referred 20aaVP3-VP1-Co1) highest level. Lowering culture temperature from 30 ⁰C further enhanced production. The level 20aaVP3-VP1-Co1 was estimated be 7.7 mg/L, is comparable other heterologous produced our system. Oral administration cell expressing induced VP1-specific IgG IgA responses in mice. cerevisiae-expressed significant immune response FMDV structural protein, opens possibility an oral vaccine.
Язык: Английский
Процитировано
1Yeasts as Potential Probiotics
Опубликована: Янв. 1, 2024
Язык: Английский
Процитировано
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