Perspectives on the Drosophila melanogaster Model for Advances in Toxicological Science
Current Protocols,
Год журнала:
2023,
Номер
3(8)
Опубликована: Авг. 1, 2023
The
use
of
Drosophila
melanogaster
for
studies
toxicology
has
grown
considerably
in
the
last
decade.
model
long
been
appreciated
as
a
versatile
and
powerful
developmental
biology
genetics
because
its
ease
handling,
short
life
cycle,
low
cost
maintenance,
molecular
genetic
accessibility,
availability
wide
range
publicly
available
strains
data
resources.
These
features,
together
with
recent
unique
developments
genomics
metabolomics,
make
fly
especially
relevant
timely
development
new
approach
methodologies
movements
toward
precision
toxicology.
Here,
we
offer
perspective
on
how
flies
can
be
leveraged
to
identify
risk
factors
environmental
exposures
human
health.
First,
review
discuss
fundamental
toxicologic
principles
experimental
design
Drosophila.
Next,
describe
quantitative
systems
approaches
resolve
architecture
candidate
pathways
controlling
susceptibility
toxicants.
Finally,
summarize
current
state
future
promise
emerging
field
metabolomics
elaborating
toxic
mechanisms.
©
2023
Authors.
Current
Protocols
published
by
Wiley
Periodicals
LLC.
Язык: Английский
Arsenic impairs Drosophila neural stem cell mitotic progression and sleep behavior in a tauopathy model
G3 Genes Genomes Genetics,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 7, 2025
Abstract
Despite
established
exposure
limits,
arsenic
remains
the
most
significant
environmental
risk
factor
detrimental
to
human
health
and
is
associated
with
carcinogenesis
neurotoxicity.
Arsenic
compromises
neurodevelopment,
it
peripheral
neuropathy
in
adults.
Exposure
heavy
metals,
such
as
arsenic,
may
also
increase
of
neurodegenerative
disorders.
Nevertheless,
molecular
mechanisms
underlying
arsenic-induced
neurotoxicity
remain
poorly
understood.
Elucidating
how
contributes
mitigate
some
risks
chronic
sublethal
inform
future
interventions.
In
this
study,
we
examine
effects
on
Drosophila
larval
neurodevelopment
adult
neurologic
function.
Consistent
prior
work,
identify
developmental
delays
heightened
mortality
response
arsenic.
Within
developing
brain,
a
dose-dependent
brain
volume.
This
aberrant
growth
coupled
impaired
mitotic
progression
neural
stem
cells
(NSCs),
progenitors
neurons
glia
central
nervous
system.
Live
imaging
cycling
NSCs
reveals
cell
cycle
upon
treatment,
leading
genomic
instability.
adults,
reduces
function,
locomotion.
Finally,
show
selectively
impairs
circadian
rhythms
humanized
tauopathy
model.
These
findings
reveal
sex-specific
genetic
vulnerabilities
exposure.
Язык: Английский
Auxin exposure disrupts feeding behavior and fatty acid metabolism in adult Drosophila
eLife,
Год журнала:
2024,
Номер
12
Опубликована: Янв. 19, 2024
The
ease
of
genetic
manipulation
in
Drosophila
melanogaster
using
the
Gal4/UAS
system
has
been
beneficial
addressing
key
biological
questions.
Current
modifications
this
methodology
to
temporally
induce
transgene
expression
require
temperature
changes
or
exposure
exogenous
compounds,
both
which
have
shown
detrimental
effects
on
physiological
processes.
recently
described
auxin-inducible
gene
(AGES)
utilizes
plant
hormone
auxin
and
is
proposed
be
least
toxic
compound
for
manipulation,
with
no
obvious
development
survival
one
wild-type
strain.
Here,
we
show
that
delays
larval
another
widely
used
fly
strain,
short-
long-term
adult
induces
observable
physiology
feeding
behavior.
We
further
reveal
a
dosage
response
upon
exposure,
recommended
concentration
AGES
alters
activity.
Furthermore,
auxin-fed
male
female
flies
exhibit
significant
decrease
triglyceride
levels
display
altered
transcription
fatty
acid
metabolism
genes.
Although
disrupted,
does
not
significantly
impact
fecundity
progeny
survival,
suggesting
may
an
ideal
studying
limited
These
results
emphasize
experiments
temporal
binary
systems
must
carefully
designed
controlled
avoid
confounding
misinterpretation
results.
Язык: Английский
Evaluation of Verbascum flower extracts as a natural source of pigments with potential health benefits
Food & Function,
Год журнала:
2024,
Номер
15(11), С. 5921 - 5928
Опубликована: Янв. 1, 2024
Crocins
are
bioactive
glucosylated
apocarotenoids
that
confer
a
yellow
pigmentation.
In
addition
to
their
coloring
ability,
crocins
offer
potential
health
benefits
because
of
antioxidant
and
anti-inflammatory
properties.
These
compounds
present
in
the
flowers
fruits
few
plant
species,
including
saffron,
gardenia,
Buddleja
Verbascum
species.
Saffron
extracts
have
been
used
for
formulation
functional
foods.
However,
there
is
no
evidence
use
other
plants
producing
food
industry.
This
study
evaluated
effect
ground
dry
two
with
activity,
as
well
fruit
powder,
from
recently
engineered
tomato
accumulate
high
levels
crocins,
ingredients
during
processing
rice,
wheat
cous-cous
maize
noodles,
providing
Correlation
analyses
revealed
increased
activity
three
matrices
was
due
presence
which
showed
toxicity.
Furthermore,
vitro
digestion
were
more
bioaccessible
rice
than
or
inferring
importance
matrix
bio
accessibility.
The
obtained
results
commercial
Verbascum's
flowers,
source
natural
pigments
activities.
Язык: Английский
Using AI to prevent the insect apocalypse: toward new environmental risk assessment procedures
Current Opinion in Insect Science,
Год журнала:
2024,
Номер
68, С. 101324 - 101324
Опубликована: Дек. 27, 2024
Insect
populations
are
declining
globally,
with
multiple
potential
drivers
identified.
However,
experimental
data
needed
to
understand
their
relative
contributions.
We
highlight
the
sublethal
effects
of
pesticides
at
field-relevant
concentrations,
often
overlooked
in
standard
environmental
risk
assessments
(ERA),
as
significant
contributors
these
declines.
Behavior,
an
easily
monitored
high-level
phenotype,
reflects
alterations
various
phenotypic
levels.
propose
incorporating
behavioral
assays
AI-based
analytical
methods
into
ERA
protocols
better
assess
safety
molecules
intended
for
large-scale
field
use.
This
approach
aims
safeguard
food
supplies
and
protect
vital
ecosystems
future.
Язык: Английский
Auxin exposure disrupts feeding behavior and fatty acid metabolism in adult Drosophila
eLife,
Год журнала:
2023,
Номер
12
Опубликована: Ноя. 6, 2023
The
ease
of
genetic
manipulation
in
Drosophila
melanogaster
using
the
Gal4/UAS
system
has
been
beneficial
addressing
key
biological
questions.
Current
modifications
this
methodology
to
temporally
induce
transgene
expression
require
temperature
changes
or
exposure
exogenous
compounds,
both
which
have
shown
detrimental
effects
on
physiological
processes.
recently
described
auxin-inducible
gene
(AGES)
utilizes
plant
hormone
auxin
and
is
proposed
be
least
toxic
compound
for
manipulation,
with
no
obvious
development
survival
one
wild-type
strain.
Here,
we
show
that
delays
larval
another
widely
used
fly
strain,
short-
long-term
adult
induces
observable
physiology
feeding
behavior.
We
further
reveal
a
dosage
response
upon
exposure,
recommended
concentration
AGES
alters
activity.
Furthermore,
auxin-fed
male
female
flies
exhibit
significant
decrease
triglyceride
levels
display
altered
transcription
fatty
acid
metabolism
genes.
Although
disrupted,
does
not
significantly
impact
fecundity
progeny
survival,
suggesting
may
an
ideal
studying
limited
These
results
emphasize
experiments
temporal
binary
systems
must
carefully
designed
controlled
avoid
confounding
misinterpretation
results.
Язык: Английский
Arsenic impairsDrosophilaneural stem cell mitotic progression and sleep behavior in a tauopathy model
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Авг. 7, 2024
Abstract
Despite
established
exposure
limits,
arsenic
remains
the
most
significant
environmental
risk
factor
detrimental
to
human
health
and
is
associated
with
carcinogenesis
neurotoxicity.
Arsenic
compromises
neurodevelopment,
it
peripheral
neuropathy
in
adults.
Exposure
heavy
metals,
such
as
arsenic,
may
also
increase
of
neurodegenerative
disorders.
Nevertheless,
molecular
mechanisms
underlying
arsenic-
induced
neurotoxicity
remain
poorly
understood.
Elucidating
how
contributes
mitigate
some
risks
chronic
sublethal
inform
future
interventions.
In
this
study,
we
examine
effects
on
Drosophila
larval
neurodevelopment
adult
neurologic
function.
Consistent
prior
work,
identify
developmental
delays
heightened
mortality
response
arsenic.
Within
developing
brain,
a
dose-dependent
brain
volume.
This
aberrant
growth
coupled
impaired
mitotic
progression
neural
stem
cells
(NSCs),
progenitors
neurons
glia
central
nervous
system.
Live
imaging
cycling
NSCs
reveals
cell
cycle
upon
treatment,
leading
genomic
instability.
adults,
reduces
function,
locomotion.
Finally,
show
selectively
impairs
circadian
rhythms
humanized
tauopathy
model.
These
findings
reveal
sex-
specific
genetic
vulnerabilities
exposure.
Язык: Английский
Drosophila Toxicogenomics: genetic variation and sexual dimorphism in susceptibility to 4-Methylimidazole
Human Genomics,
Год журнала:
2024,
Номер
18(1)
Опубликована: Ноя. 4, 2024
Abstract
Background
4-methylimidazole
is
a
ubiquitous
and
potentially
carcinogenic
environmental
toxicant.
Genetic
factors
that
contribute
to
variation
in
susceptibility
its
toxic
effects
are
challenging
assess
human
populations.
We
used
the
Drosophila
melanogaster
Reference
Panel
(DGRP),
living
library
of
natural
genetic
variation,
identify
genes
with
orthologs
associated
4-methylimidazole.
Results
screened
204
DGRP
lines
for
survival
following
24-hour
exposure
found
extensive
survival,
broad
sense
heritability
0.82;
as
well
sexual
dimorphism,
cross-sex
correlation
0.59.
Genome-wide
association
analyses
identified
total
241
candidate
molecular
polymorphisms
or
near
273
unique
survival.
These
had
either
sex-specific
sex-antagonistic
effects,
most
putative
regulatory
effects.
generated
interaction
networks
using
these
inputs
computationally
recruited
known
physical
interactions.
The
network
were
significantly
over-represented
gene
ontology
terms
involving
all
aspects
development
(including
nervous
system
development)
cellular
organismal
functions
canonical
signaling
pathways,
orthologs.
Conclusions
basis
sensitivity
acute
attributable
their
on
multiple
developmental
processes,
including
possible
neurotoxicity.
Given
evolutionary
conservation
underlying
insights
may
be
applicable
humans.
Язык: Английский
Auxin Exposure Disrupts Feeding Behavior and Fatty Acid Metabolism in AdultDrosophila
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Авг. 16, 2023
ABSTRACT
The
ease
of
genetic
manipulation
in
Drosophila
melanogaster
using
the
Gal4/UAS
system
has
been
beneficial
addressing
key
biological
questions.
Current
modifications
this
methodology
to
temporally
induce
transgene
expression
require
temperature
changes
or
exposure
exogenous
compounds,
both
which
have
shown
detrimental
effects
on
physiological
processes.
recently
described
auxin-inducible
gene
(AGES)
utilizes
plant
hormone
auxin
and
is
proposed
be
least
toxic
compound
for
manipulation,
with
no
obvious
development
survival
one
wild-type
strain.
Here
we
show
that
delays
larval
another
widely-used
fly
strain,
short-
long-term
adult
induces
observable
physiology
feeding
behavior.
We
further
reveal
a
dosage
response
upon
exposure,
recommended
concentration
AGES
alters
activity.
Furthermore,
fed
male
female
flies
exhibit
significant
decrease
triglyceride
levels
display
altered
transcription
fatty
acid
metabolism
genes.
Although
disrupted,
does
not
significantly
impact
fecundity
progeny
survival,
suggesting
may
an
ideal
studying
limited
These
results
emphasize
experiments
temporal
binary
systems
must
carefully
designed
controlled
avoid
confounding
misinterpretation
results.
Язык: Английский