Auxin Exposure Disrupts Feeding Behavior and Fatty Acid Metabolism in AdultDrosophila DOI Creative Commons
Sophie A. Fleck, Puja Biswas,

Emily D. DeWitt

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 16, 2023

ABSTRACT The ease of genetic manipulation in Drosophila melanogaster using the Gal4/UAS system has been beneficial addressing key biological questions. Current modifications this methodology to temporally induce transgene expression require temperature changes or exposure exogenous compounds, both which have shown detrimental effects on physiological processes. recently described auxin-inducible gene (AGES) utilizes plant hormone auxin and is proposed be least toxic compound for manipulation, with no obvious development survival one wild-type strain. Here we show that delays larval another widely-used fly strain, short- long-term adult induces observable physiology feeding behavior. We further reveal a dosage response upon exposure, recommended concentration AGES alters activity. Furthermore, fed male female flies exhibit significant decrease triglyceride levels display altered transcription fatty acid metabolism genes. Although disrupted, does not significantly impact fecundity progeny survival, suggesting may an ideal studying limited These results emphasize experiments temporal binary systems must carefully designed controlled avoid confounding misinterpretation results.

Language: Английский

Perspectives on the Drosophila melanogaster Model for Advances in Toxicological Science DOI Creative Commons
Matthew D. Rand, Jason M. Tennessen,

Trudy F. C. Mackay

et al.

Current Protocols, Journal Year: 2023, Volume and Issue: 3(8)

Published: Aug. 1, 2023

The use of Drosophila melanogaster for studies toxicology has grown considerably in the last decade. model long been appreciated as a versatile and powerful developmental biology genetics because its ease handling, short life cycle, low cost maintenance, molecular genetic accessibility, availability wide range publicly available strains data resources. These features, together with recent unique developments genomics metabolomics, make fly especially relevant timely development new approach methodologies movements toward precision toxicology. Here, we offer perspective on how flies can be leveraged to identify risk factors environmental exposures human health. First, review discuss fundamental toxicologic principles experimental design Drosophila. Next, describe quantitative systems approaches resolve architecture candidate pathways controlling susceptibility toxicants. Finally, summarize current state future promise emerging field metabolomics elaborating toxic mechanisms. © 2023 Authors. Current Protocols published by Wiley Periodicals LLC.

Language: Английский

Citations

25
Arsenic impairs Drosophila neural stem cell mitotic progression and sleep behavior in a tauopathy model DOI Creative Commons
Temitope H. Adebambo, Fernanda Medina‐Flores, Shirley Zhang

et al.

G3 Genes Genomes Genetics, Journal Year: 2025, Volume and Issue: unknown

Published: April 7, 2025

Abstract Despite established exposure limits, arsenic remains the most significant environmental risk factor detrimental to human health and is associated with carcinogenesis neurotoxicity. Arsenic compromises neurodevelopment, it peripheral neuropathy in adults. Exposure heavy metals, such as arsenic, may also increase of neurodegenerative disorders. Nevertheless, molecular mechanisms underlying arsenic-induced neurotoxicity remain poorly understood. Elucidating how contributes mitigate some risks chronic sublethal inform future interventions. In this study, we examine effects on Drosophila larval neurodevelopment adult neurologic function. Consistent prior work, identify developmental delays heightened mortality response arsenic. Within developing brain, a dose-dependent brain volume. This aberrant growth coupled impaired mitotic progression neural stem cells (NSCs), progenitors neurons glia central nervous system. Live imaging cycling NSCs reveals cell cycle upon treatment, leading genomic instability. adults, reduces function, locomotion. Finally, show selectively impairs circadian rhythms humanized tauopathy model. These findings reveal sex-specific genetic vulnerabilities exposure.

Language: Английский

Citations

0
Auxin exposure disrupts feeding behavior and fatty acid metabolism in adult Drosophila DOI Creative Commons
Sophie A. Fleck, Puja Biswas,

Emily D. DeWitt

et al.

eLife, Journal Year: 2024, Volume and Issue: 12

Published: Jan. 19, 2024

The ease of genetic manipulation in Drosophila melanogaster using the Gal4/UAS system has been beneficial addressing key biological questions. Current modifications this methodology to temporally induce transgene expression require temperature changes or exposure exogenous compounds, both which have shown detrimental effects on physiological processes. recently described auxin-inducible gene (AGES) utilizes plant hormone auxin and is proposed be least toxic compound for manipulation, with no obvious development survival one wild-type strain. Here, we show that delays larval another widely used fly strain, short- long-term adult induces observable physiology feeding behavior. We further reveal a dosage response upon exposure, recommended concentration AGES alters activity. Furthermore, auxin-fed male female flies exhibit significant decrease triglyceride levels display altered transcription fatty acid metabolism genes. Although disrupted, does not significantly impact fecundity progeny survival, suggesting may an ideal studying limited These results emphasize experiments temporal binary systems must carefully designed controlled avoid confounding misinterpretation results.

Language: Английский

Citations

1
Evaluation of Verbascum flower extracts as a natural source of pigments with potential health benefits DOI Creative Commons
Lucía Morote, Cristián Martinez Fajardo,

Eduardo Parreño

et al.

Food & Function, Journal Year: 2024, Volume and Issue: 15(11), P. 5921 - 5928

Published: Jan. 1, 2024

Crocins are bioactive glucosylated apocarotenoids that confer a yellow pigmentation. In addition to their coloring ability, crocins offer potential health benefits because of antioxidant and anti-inflammatory properties. These compounds present in the flowers fruits few plant species, including saffron, gardenia, Buddleja Verbascum species. Saffron extracts have been used for formulation functional foods. However, there is no evidence use other plants producing food industry. This study evaluated effect ground dry two with activity, as well fruit powder, from recently engineered tomato accumulate high levels crocins, ingredients during processing rice, wheat cous-cous maize noodles, providing Correlation analyses revealed increased activity three matrices was due presence which showed toxicity. Furthermore, vitro digestion were more bioaccessible rice than or inferring importance matrix bio accessibility. The obtained results commercial Verbascum's flowers, source natural pigments activities.

Language: Английский

Citations

1
Using AI to prevent the insect apocalypse: toward new environmental risk assessment procedures DOI Creative Commons
Lautaro Gándara, Amy Foreman, Justin Crocker

et al.

Current Opinion in Insect Science, Journal Year: 2024, Volume and Issue: 68, P. 101324 - 101324

Published: Dec. 27, 2024

Insect populations are declining globally, with multiple potential drivers identified. However, experimental data needed to understand their relative contributions. We highlight the sublethal effects of pesticides at field-relevant concentrations, often overlooked in standard environmental risk assessments (ERA), as significant contributors these declines. Behavior, an easily monitored high-level phenotype, reflects alterations various phenotypic levels. propose incorporating behavioral assays AI-based analytical methods into ERA protocols better assess safety molecules intended for large-scale field use. This approach aims safeguard food supplies and protect vital ecosystems future.

Language: Английский

Citations

1
Auxin exposure disrupts feeding behavior and fatty acid metabolism in adult Drosophila DOI Creative Commons
Sophie A. Fleck, Puja Biswas,

Emily D. DeWitt

et al.

eLife, Journal Year: 2023, Volume and Issue: 12

Published: Nov. 6, 2023

The ease of genetic manipulation in Drosophila melanogaster using the Gal4/UAS system has been beneficial addressing key biological questions. Current modifications this methodology to temporally induce transgene expression require temperature changes or exposure exogenous compounds, both which have shown detrimental effects on physiological processes. recently described auxin-inducible gene (AGES) utilizes plant hormone auxin and is proposed be least toxic compound for manipulation, with no obvious development survival one wild-type strain. Here, we show that delays larval another widely used fly strain, short- long-term adult induces observable physiology feeding behavior. We further reveal a dosage response upon exposure, recommended concentration AGES alters activity. Furthermore, auxin-fed male female flies exhibit significant decrease triglyceride levels display altered transcription fatty acid metabolism genes. Although disrupted, does not significantly impact fecundity progeny survival, suggesting may an ideal studying limited These results emphasize experiments temporal binary systems must carefully designed controlled avoid confounding misinterpretation results.

Language: Английский

Citations

2
Arsenic impairsDrosophilaneural stem cell mitotic progression and sleep behavior in a tauopathy model DOI Creative Commons
Temitope H. Adebambo,

M Flores,

Shirley Zhang

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 7, 2024

Abstract Despite established exposure limits, arsenic remains the most significant environmental risk factor detrimental to human health and is associated with carcinogenesis neurotoxicity. Arsenic compromises neurodevelopment, it peripheral neuropathy in adults. Exposure heavy metals, such as arsenic, may also increase of neurodegenerative disorders. Nevertheless, molecular mechanisms underlying arsenic- induced neurotoxicity remain poorly understood. Elucidating how contributes mitigate some risks chronic sublethal inform future interventions. In this study, we examine effects on Drosophila larval neurodevelopment adult neurologic function. Consistent prior work, identify developmental delays heightened mortality response arsenic. Within developing brain, a dose-dependent brain volume. This aberrant growth coupled impaired mitotic progression neural stem cells (NSCs), progenitors neurons glia central nervous system. Live imaging cycling NSCs reveals cell cycle upon treatment, leading genomic instability. adults, reduces function, locomotion. Finally, show selectively impairs circadian rhythms humanized tauopathy model. These findings reveal sex- specific genetic vulnerabilities exposure.

Language: Английский

Citations

0
Drosophila Toxicogenomics: genetic variation and sexual dimorphism in susceptibility to 4-Methylimidazole DOI Creative Commons
Karen Collins,

Elisabeth Howansky,

Sarah C. Macon-Foley

et al.

Human Genomics, Journal Year: 2024, Volume and Issue: 18(1)

Published: Nov. 4, 2024

Abstract Background 4-methylimidazole is a ubiquitous and potentially carcinogenic environmental toxicant. Genetic factors that contribute to variation in susceptibility its toxic effects are challenging assess human populations. We used the Drosophila melanogaster Reference Panel (DGRP), living library of natural genetic variation, identify genes with orthologs associated 4-methylimidazole. Results screened 204 DGRP lines for survival following 24-hour exposure found extensive survival, broad sense heritability 0.82; as well sexual dimorphism, cross-sex correlation 0.59. Genome-wide association analyses identified total 241 candidate molecular polymorphisms or near 273 unique survival. These had either sex-specific sex-antagonistic effects, most putative regulatory effects. generated interaction networks using these inputs computationally recruited known physical interactions. The network were significantly over-represented gene ontology terms involving all aspects development (including nervous system development) cellular organismal functions canonical signaling pathways, orthologs. Conclusions basis sensitivity acute attributable their on multiple developmental processes, including possible neurotoxicity. Given evolutionary conservation underlying insights may be applicable humans.

Language: Английский

Citations

0
Auxin Exposure Disrupts Feeding Behavior and Fatty Acid Metabolism in AdultDrosophila DOI Creative Commons
Sophie A. Fleck, Puja Biswas,

Emily D. DeWitt

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 16, 2023

ABSTRACT The ease of genetic manipulation in Drosophila melanogaster using the Gal4/UAS system has been beneficial addressing key biological questions. Current modifications this methodology to temporally induce transgene expression require temperature changes or exposure exogenous compounds, both which have shown detrimental effects on physiological processes. recently described auxin-inducible gene (AGES) utilizes plant hormone auxin and is proposed be least toxic compound for manipulation, with no obvious development survival one wild-type strain. Here we show that delays larval another widely-used fly strain, short- long-term adult induces observable physiology feeding behavior. We further reveal a dosage response upon exposure, recommended concentration AGES alters activity. Furthermore, fed male female flies exhibit significant decrease triglyceride levels display altered transcription fatty acid metabolism genes. Although disrupted, does not significantly impact fecundity progeny survival, suggesting may an ideal studying limited These results emphasize experiments temporal binary systems must carefully designed controlled avoid confounding misinterpretation results.

Language: Английский

Citations

1