Circular RNAs: history, metabolism, mechanisms of function, and regulatory roles at a glance DOI Open Access
Roqaye Karimi, Ehsan Javandoost, Nooshin Asadmasjedi

и другие.

Annals of Medicine and Surgery, Год журнала: 2024, Номер 87(1), С. 141 - 150

Опубликована: Дек. 19, 2024

Circular RNAs (circRNAs) are non-coding RNA (ncRNA) molecules that, due to their covalent ring structure and lack of free ends, have a very high intracellular stability compared linear counterparts. In general, circRNAs expressed in mammalian cells exhibit tissue/cell-specific expression patterns. Mounting evidence is indicative that regulate variety cellular processes by acting as miRNA sponges, transcriptional regulators, protein molecular scaffolds, protein/peptide translators. The emergence the biological functions has brought novel outlook our better understanding physiology disease pathogenesis. CircRNAs also been shown play critical role occurrence, development progression cancers. Their participation pathophysiology various diseases including cardiovascular diseases, diabetes neurological disorders important. Such characteristics led more studies investigating promising tools medicine targeted therapy.

Язык: Английский

Research trends and hotspots of circular RNA in cardiovascular disease: A bibliometric analysis DOI Creative Commons

Zehui Xu,

Chong Guan,

Ziji Cheng

и другие.

Non-coding RNA Research, Год журнала: 2024, Номер 9(3), С. 930 - 944

Опубликована: Апрель 17, 2024

From a global perspective, cardiovascular diseases (CVDs), the leading factor accounting for population mortality, and circRNAs, RNA molecules with stable closed-loop structures, have been proven to be closely related. The latent clinical value potential role of circRNAs in CVDs attracting increasing, active research interest, but bibliometric studies this field are still lacking. Thus, study, we conducted analysis by using software such as VOSviewer, CiteSpace, Microsoft Excel, R package determine current progress hotspots ultimately provide an overview development trends future frontiers field. In our based on search strategy, total 1206 publications published before July 31, 2023 were accessed from WOSCC database. According findings, there is notable increasing trend circRNA CVDs. China was found dominant country terms publication number, lack high-quality articles significant fault. A cluster co-cited references indicated that dilated cardiomyopathy, AMI, cardiac hypertrophy greatest objects concern. contrast, keywords high importance has ascribed MI, abdominal aortic aneurysm, cell proliferation, coronary artery diseases.

Язык: Английский

Процитировано

4
CircRNAs in Colorectal Cancer: Unveiling Their Roles and Exploring Therapeutic Potential DOI

Yihan Ding,

Xiaofei Song, Jingsong Chen

и другие.

Biochemical Genetics, Год журнала: 2025, Номер unknown

Опубликована: Март 3, 2025

Язык: Английский

Процитировано

0
Purifying circular RNA by ultrafiltration DOI Creative Commons

Karen Guillen-Cuevas,

Xiaoming Lu, Marc R. Birtwistle

и другие.

Separation and Purification Technology, Год журнала: 2025, Номер 366, С. 132809 - 132809

Опубликована: Апрель 4, 2025

Язык: Английский

Процитировано

0
From non‐coding RNAs to cancer regulators: The fascinating world of micropeptides DOI
Can Li, Dan Zhang, Jinxi Huang

и другие.

International Journal of Cancer, Год журнала: 2025, Номер unknown

Опубликована: Апрель 25, 2025

Abstract Micropeptides are commonly identified as peptides encoded by non‐coding RNAs (ncRNAs). In the short open reading frame (sORF) of ncRNAs, there is a base sequence encoding functional micropeptides, which great significance in biological field. Recently, micropeptides regulate diverse processes, including mitochondrial metabolism, calcium transport, mRNA splicing, signal transduction, myocyte fusion, and cellular senescence, regulating homeostasis internal environment cancer's incidence progression. Especially, study cancer about potential regulatory mechanism will be conducive to further understanding process initiation development. More more research shows have been confirmed play an essential role emergence multiple kinds cancers, Breast cancer, Colon Colorectal Glioma, Glioblastoma, Liver cancer. This review presents comprehensive synthesis latest advancements our roles cells, with particular focus on networks involving oncogenesis. The new mode action provides innovative ideas for diagnosis treatment. Moreover, we explored significant capacity diagnostic biomarkers targets anti‐cancer therapies clinical settings, highlighting their development micropeptide‐based tools therapeutics.

Язык: Английский

Процитировано

0
Emerging roles of exosomal circRNAs in non-small cell lung cancer DOI Creative Commons
Hong Yin, Jiaxiao Shi, Shaoling Li

и другие.

Journal of Translational Medicine, Год журнала: 2025, Номер 23(1)

Опубликована: Апрель 30, 2025

Despite the prevalence of non-small cell lung cancer (NSCLC) is high, limited early detection and management these tumors are restricted since there an absence reliable precise diagnostic biomarkers therapeutic targets. Exosomes transport functional molecules for facilitating intercellular communication, especially in tumor microenvironment, indicating their potential as Circular RNA (circRNA), a type non-coding possessing covalently closed loop structure, substantial abundance, tissue-specific expression patterns, stably enriched exosomes. In recent years, significant breakthroughs have been made research on exosomal circRNA NSCLC. This review briefly introduces biogenesis, characterizations, functions circRNAs exosomes, systematically describes biological mechanisms addition, this study summarizes role progression NSCLC discusses clinical significance targets

Язык: Английский

Процитировано

0
Three-dimensional in vitro models in head and neck cancer: current trends and applications DOI

Masoumeh Amiri,

Tayebeh Sadat Tabatabai,

Zahra Seifi

и другие.

Medical Oncology, Год журнала: 2025, Номер 42(6)

Опубликована: Май 5, 2025

Язык: Английский

Процитировано

0
Preliminary Evaluation of Plasma circ_0009910, circ_0027478, and miR-1236-3p as Diagnostic and Prognostic Biomarkers in Hepatocellular Carcinoma DOI Open Access

Mona Samy Awed,

Abeer Ibrahim,

Omnia Ezzat

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(10), С. 4842 - 4842

Опубликована: Май 19, 2025

Circular RNAs (circRNAs) are increasingly recognized as significant regulators in multiple cancers, such hepatocellular carcinoma (HCC), frequently affecting microRNA (miRNA) expression. The diagnostic and prognostic roles of circRNAs, specifically circ_0009910 circ_0027478, conjunction with miR-1236-3p, HCC, have not yet been fully investigated. In this pilot study, we assessed the expression levels circ_0009910, miR-1236-3p plasma samples from 100 patients diagnosed HCC 50 healthy controls through reverse transcriptase–quantitative polymerase chain reaction (RT-qPCR). performance was evaluated using receiver operating characteristic (ROC) curve analysis, correlations clinicopathological features were examined. Circ_0009910 circ_0027478 exhibited upregulation (p < 0.05), whereas demonstrated downregulation 0.05). accuracy (area under [AUC] = 0.90), effectively differentiating showing a correlation tumor size, metastasis, alpha-fetoprotein (AFP) Both positive features, an inverse correlation. Logistic regression validated capabilities these biomarkers. results indicate that AFP three, present promising profile for HCC. Additional validation larger cohorts is required to establish their clinical utility.

Язык: Английский

Процитировано

0
Circular RNAs, Noncoding RNAs, and N6-methyladenosine Involved in the Development of MAFLD DOI Creative Commons

Moeka Nakashima,

Naoko Suga,

Yuka Ikeda

и другие.

Non-Coding RNA, Год журнала: 2024, Номер 10(1), С. 11 - 11

Опубликована: Фев. 5, 2024

Noncoding RNAs (ncRNAs), including circular (circRNAs) and N6-methyladenosine (m6A), have been shown to play a critical role in the development of various diseases obesity metabolic disorder-associated fatty liver disease (MAFLD). Obesity is chronic caused by excessive fat accumulation body, which has recently become more prevalent foremost risk factor for MAFLD. Causes may involve interaction genetic, behavioral, social factors. m6A RNA methylation might add novel inspiration understanding MAFLD with post-transcriptional regulation gene expression. In particular, circRNAs, microRNAs (miRNAs), be implicated progression Interestingly, modification can modulate translation, degradation, other functions ncRNAs. miRNAs/circRNAs also modifications affecting writers, erasers, readers. turn, ncRNAs could expression regulators different ways. However, there limited evidence on how these interact affect promotion diseases. It seems that occur DNA, RNA, proteins associated several biological properties. This study provides mechanistic association diseases, especially Comprehension between contribute treatment tactics

Язык: Английский

Процитировано

3
circCUL3 drives malignant progression of cervical cancer by activating autophagy through sponge miR-223-3p upregulation of ATG7 DOI

Jiahui Qin,

Yan Chen, Xia Zhao

и другие.

Gene, Год журнала: 2024, Номер 925, С. 148572 - 148572

Опубликована: Май 15, 2024

Язык: Английский

Процитировано

2
Purifying circular RNA by ultrafiltration DOI Open Access

Karen Guillen-Cuevas,

Xiaoming Lu, Marc R. Birtwistle

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Дек. 7, 2024

Abstract Developing messenger RNA (mRNA) vaccines for COVID-19 renewed and intensified the interest in using mRNA disease prevention treatment. Despite their efficacy, linear molecules are short-lived human body, primarily due to enzymatic degradation at free ends. In contrast, circular (circRNA) exhibits enhanced stability resistance exonuclease degradation. However, this depends highly on purity. Unfortunately, vitro transcription/self-splicing reaction products contain a mixture of RNAs. Yet, practical methods purifying circRNA from solutions containing contaminants lacking. study, we explored feasibility ultrafiltration purify protein-encoding produced by self-splicing precursor (preRNA) during transcription (IVT). We measured sieving coefficients, separation metric, circRNA, RNA, nicked conformers polyethersulfone membranes with molecular weight cutoffs 30 300 kDa, analyzing performance as function permeate flux. also estimated critical fluxes determined suitable operating conditions purification. achieved purity 86% yield above 50%. By comparison, size-exclusion high-performance liquid chromatography (SE-HPLC), leading alterative technology, was 41% 45%. These findings highlight superior method research scale. Its scalability suggests that it could play role enabling large-scale manufacturing circRNA-based therapeutics.

Язык: Английский

Процитировано

1