Cognitive Neurodynamics, Год журнала: 2023, Номер 18(2), С. 581 - 595
Опубликована: Окт. 9, 2023
Язык: Английский
Materials Today Bio, Год журнала: 2022, Номер 18, С. 100522 - 100522
Опубликована: Дек. 21, 2022
Extracellular vesicles (EVs) are a collective term for nanoscale or microscale secreted by cells that play important biological roles. Mesenchymal stem class of with the potential self-healing and multidirectional differentiation. In recent years, numerous studies have shown EVs, especially those mesenchymal cells, can promote repair regeneration various tissues and, thus, significant in regenerative medicine. However, due to rapid clearance capacity circulatory system, EVs barely able act persistently at specific sites target tissues. Hydrogels good biocompatibility loose porous structural properties allow them serve as EV carriers, thereby prolonging retention certain areas slowing release EVs. When needed function sites, EV-loaded hydrogels stand an excellent approach. this review, we first introduce sources, roles, extraction characterization methods describe their current application status. We then review different types discuss factors influencing abilities carry summarize several strategies loading into characterizing hydrogels. Furthermore, applications tissue repair. This article concludes summary state research on outlook future directions, which hope will provide promising ideas researchers.
Язык: Английский
Процитировано
259
Nature reviews. Neuroscience, Год журнала: 2023, Номер 24(3), С. 134 - 152
Опубликована: Янв. 18, 2023
Язык: Английский
Процитировано
70
International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(15), С. 12046 - 12046
Опубликована: Июль 27, 2023
Although historically, the traditional bidimensional in vitro cell system has been widely used research, providing much fundamental information regarding cellular functions and signaling pathways as well nuclear activities, simplicity of this does not fully reflect heterogeneity complexity vivo systems. From arises need to use animals for experimental research testing. Nevertheless, animal experimentation presents various aspects complexity, such ethical issues, which led Russell Burch 1959 formulate 3R (Replacement, Reduction, Refinement) principle, underlying urgent introduce non-animal-based methods research. Considering this, three-dimensional (3D) models emerged scientific community a bridge between models, allowing achievement differentiation while avoiding The purpose review is provide general overview most common establish 3D culture discuss their promising applications. Three-dimensional cultures have employed study both organ physiology diseases; moreover, they represent valuable tool studying many cancer. Finally, possibility using drug screening regenerative medicine paves way development new therapeutic opportunities diseases.
Язык: Английский
Процитировано
50
European journal of medical research, Год журнала: 2024, Номер 29(1)
Опубликована: Июль 25, 2024
Stem cell-based therapies have emerged as a promising approach for treating various neurological disorders by harnessing the regenerative potential of stem cells to restore damaged neural tissue and circuitry. This comprehensive review provides an in-depth analysis current state cell applications in primary conditions, including Parkinson's disease (PD), Alzheimer's (AD), amyotrophic lateral sclerosis (ALS), multiple (MS), stroke, spinal cord injury (SCI), other related disorders. The begins with detailed introduction biology, discussing types, sources, mechanisms action therapies. It then critically examines preclinical evidence from animal models early human trials investigating safety, feasibility, efficacy different such embryonic (ESCs), mesenchymal (MSCs), (NSCs), induced pluripotent (iPSCs). While ESCs been studied extensively models, clinical primarily focused on adult MSCs NSCs, well iPSCs their derivatives. We assess research each type, highlighting limitations conditions. synthesizes key findings recent, high-quality studies condition, manufacturing, delivery methods, therapeutic outcomes. replace lost neurons directly reconstruct circuits is highlighted, emphasizes critical role paracrine immunomodulatory mediating effects most article also explores challenges associated translating into practice, issues sourcing, scalability, regulatory considerations. Furthermore, it discusses future directions opportunities advancing treatments, gene editing, biomaterials, personalized iPSC-derived therapies, novel strategies. concludes emphasizing transformative revolutionizing treatment while acknowledging need rigorous trials, standardized protocols, multidisciplinary collaboration realize full promise.
Язык: Английский
Процитировано
23
Journal of Neural Transmission, Год журнала: 2024, Номер 131(12), С. 1367 - 1387
Опубликована: Авг. 7, 2024
Millions of individuals around the world are afflicted with Parkinson's disease (PD), a prevalent and incapacitating neurodegenerative disorder. Dr. Reichmann, distinguished professor neurologist, has made substantial advancements in domain PD research, encompassing both fundamental scientific investigations practical applications. His research illuminated etiology treatment PD, as well function energy metabolism premotor symptoms. As precursor to number neurotransmitters neuromodulators that implicated pathophysiology he also investigated application tryptophan (Trp) derivatives disease. principal findings insights summarized synthesized this narrative review article, which emphasizes challenges implications for future research. This aims identify analyze key contributions Reichmann field ultimate goal informing directions domain. By examining Reichmann's work, study seeks provide comprehensive understanding his major how they can be applied advance diagnosis PD. paper explores potential intersection emerging avenues, such investigation Trp its metabolites, particularly kynurenines, could lead new therapeutic strategies managing disorders like
Язык: Английский
Процитировано
10
Burns & Trauma, Год журнала: 2024, Номер 12
Опубликована: Янв. 1, 2024
Abstract Extracellular vesicles (EVs) are heterogeneous membrane-like secreted by living cells that involved in many physiological and pathological processes act as intermediaries of intercellular communication molecular transfer. Recent studies have shown EVs from specific sources regulate tissue repair regeneration delivering proteins, lipids, nucleic acids to target signaling molecules. Nanotechnology breakthroughs facilitated the development exploration engineered for repair. Enhancements through gene editing, surface modification, content modification further improved their therapeutic efficacy. This review summarizes potential regeneration, mechanisms action, research progress regenerative medicine. highlights design logic typical examples explores prospects The aim this is provide new insights into applications, thereby expanding use
Язык: Английский
Процитировано
10
CNS Neuroscience & Therapeutics, Год журнала: 2024, Номер 30(3)
Опубликована: Март 1, 2024
Abstract Background With the widespread prevalence of neurodegenerative diseases (NDs) and high rates mortality disability, it is imminent to find accurate targets for intervention. There growing evidence that neuroimmunity pivotal in pathology NDs interventions targeting hold great promise. Exogenous or dislocated nucleic acids activate cytosolic DNA sensor cyclic GMP‐AMP synthase (cGAS), activating stimulator interferon genes (STING). The activated STING triggers innate immune responses then cGAS‐STING signaling pathway links abnormal acid sensing response. Recently, numerous studies have shown neuroinflammation regulated by plays an essential role NDs. Aims In this review, we summarized mechanism focused on inhibitors cGAS‐STING. Conclusion important pathogenesis Inhibiting may provide new measures treatment
Язык: Английский
Процитировано
9
Biomedicines, Год журнала: 2025, Номер 13(1), С. 84 - 84
Опубликована: Янв. 1, 2025
Despite the massive efforts of modern medicine to stop evolution Alzheimer’s disease (AD), it affects an increasing number people, changing individual lives and imposing itself as a burden on families health systems. Considering that vast majority conventional drug therapies did not lead expected results, this review will discuss newly developing alternative in effort or slow AD. Focused Ultrasound (FUS) its derived Transcranial Pulse Stimulation (TPS) are non-invasive therapeutic approaches. Singly applied technique change permeability blood–brain–barrier (BBB), FUS TPS have demonstrated benefits use treating AD animal human studies. Adipose-derived stem Cells (ADSCs), gene therapy, many other methods (diet, sleep pattern, physical exercise, nanoparticle delivery) also new potential treatments since multimodal approaches represent trend disorder research therapies.
Язык: Английский
Процитировано
0
Ageing and Neurodegenerative Diseases, Год журнала: 2025, Номер 5(1)
Опубликована: Янв. 26, 2025
With aging, neural stem cells (NSCs) undergo age-related changes, including metabolic abnormalities, disrupted protein homeostasis, mitochondrial dysfunction, reduced genetic stability, and more notably, a diminished capacity for proliferation differentiation. These changes may contribute to the development of neurodegenerative diseases such as Alzheimer’s disease (AD) Parkinson’s (PD). In these conditions, disease-specific pathological interact with alterations NSCs, resulting in impairment neurogenesis, which further leads cognitive, mood, motor decline. Based on potential therapeutics targeting neurogenesis cell-based therapies restore functions NSCs replace degenerated neurons, aiming ameliorate functional decline diseases. While therapies, cell transplantation secretome therapy, show great treatment diseases, challenges tumorigenesis, immune rejection, extraction or storage need be addressed. A better understanding age- disease-related underlying mechanism driving benefits drawbacks therapeutic approaches provide insights novel disease-modifying interventions future
Язык: Английский
Процитировано
0
Journal of Materials Science Materials in Medicine, Год журнала: 2024, Номер 35(1)
Опубликована: Июнь 20, 2024
Abstract Tissue engineering holds promise for developing therapeutic applications using viscous materials e.g. hydrogels. However, assessing the cytotoxicity of such with conventional assays can be challenging due to non-specific interactions. To address this, we optimized a live/dead staining method quantitative evaluation and compared it CCK8 assay. Our MicroDrop involved seeding droplets containing 5000 cells in 10 µl medium on 12-well plates. After allowing them adhere 4 h, various samples were applied measurements conducted fluorescence microscope immediately at daily intervals up 72 h. A sodium dodecyl sulfate (SDS) dilution series The findings revealed cell-type specific pattern mg/ml hyaluronic acid (HA), wherein MC3T3-E1 maintained 95% viability until while L929 experienced gradual decline 17%. 2 HA exhibited consistent above 90% across all time points cell lines. Similarly, fibrin demonstrated dilutions points, except undiluted showing decrease from 85% 20%. Gelatin-methacrylol sustained 70% both 5% 10% concentrations. comparison SDS between (MicroDrop) metabolic activity (CCK8) assay showed correlation coefficient 0.95. study validates feasibility established assay, providing researchers an efficient tool materials. Additionally, potential yield more precise data well-known Graphical
Язык: Английский
Процитировано
3