Antioxidant production promotes defense mechanism and different gene expression level in Zea mays under abiotic stress
Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Март 26, 2024
Abstract
The
growth
and
productivity
of
maize
are
severely
affected
by
soil
salinity.
crucial
determinants
for
the
future
performance
plants
productive
seed
germination
seedling
establishment;
however,
both
stages
liable
to
For
grain,
is
an
economically
significant
crop
sensitive
abiotic
stresses.
However,
little
known
about
defense
responses
salinity-induced
antioxidant
oxidative
stress
in
maize.
In
our
work,
commercially
available
variety
Raka-Poshi
was
grown
pots
30
days
under
greenhouse
conditions.
To
evaluate
salt-induced
oxidative/antioxidant
salt
0,
25,
50,
75,
100
150
mM
concentrations,
treatments
were
provided
using
sodium
chloride
(NaCl).
All
biochemical
indices
calculated
all
NaCl
while
drought
induced
up
50%
irrigation
water.
After
germination,
leaves
collected
measurement
lipid
peroxidase
or
malondialdehyde
(MDA),
glutathione
reductase
(GR),
guaiacol
(GPOD),
hydrogen
peroxide
(H
2
O
),
superoxide
dismutase
(SOD),
lipoxygenase
(LOX),
catalase
(CAT),
ascorbate
(APOD)
glutathione-
S
-transferase
(GST).
results
revealed
a
47%
reduction
have
shown
that
successive
increase
concentrations
caused
production.
With
concentration
stress,
lower
levels
H
,
SOD,
MDA
detected
leaves.
regarding
morphology
seedlings
indicated
root
length
shoot
applications
root-to-shoot
weights
found
be
increased
decreased
conditions
During
gene
expression
analysis
collectively
indicate
that,
conditions,
nine
mentioned
enzyme-related
genes
consistently
downregulated.
Язык: Английский
Genome wide identification of phenylalanine ammonia-lyase (PAL) gene family in Cucumis sativus (cucumber) against abiotic stress
BMC Genomic Data,
Год журнала:
2024,
Номер
25(1)
Опубликована: Авг. 26, 2024
Phenylalanine
ammonia
lyase
(PAL)
is
a
widely
studied
enzyme
in
plant
biology
due
to
its
role
connecting
primary
metabolism
secondary
phenylpropanoid
metabolism,
significantly
influencing
growth,
development,
and
stress
response.
Although
PAL
genes
have
been
extensively
various
species
but
their
exploration
cucumber
has
limited.
This
study
successfully
identified
11
CsPAL
Cucumis
sativus
(cucumber).
These
were
categorized
based
on
conserved
sequences
revealing
patterns
through
MEME
analysis
multiple
sequence
alignment.
Interestingly,
cis-elements
related
found
the
promoter
regions
of
genes,
indicating
involvement
responding
abiotic
stress.
Furthermore,
these
gene's
promoters
contained
components
associated
with
light,
development
hormone
responsiveness.
suggests
that
they
may
roles
developmental
processes.
MicroRNAs
as
key
regulators
for
playing
crucial
modulating
expression.
discovery
underscores
complex
regulatory
network
involved
plant's
response
conditions.
The
influence
microRNAs
further
highlights
complicated
mechanisms
plants
use
manage
Gene
expression
analyzed
using
RNA-seq
data.
significant
upregulation
CsPAL9
during
HT3h
(heat
3
h)
heightened
both
CsPAL7
under
HT6h
6
transcriptome
suggest
potential
cucumber's
tolerance
heat
comprehensive
investigation
aims
enhance
our
understanding
gene
family's
versatility,
offering
valuable
insights
advancements
genetics.
Язык: Английский
Advances in CRISPR/Cas9 technology: shaping the future of photosynthetic microorganisms for biofuel production
Functional Plant Biology,
Год журнала:
2025,
Номер
52(2)
Опубликована: Фев. 10, 2025
Use
of
fossil
fuels
causes
environmental
issues
due
to
its
inefficiency
and
imminent
depletion.
This
has
led
interest
in
identifying
alternative
renewable
energy
sources
such
as
biofuel
generation
from
photosynthetic
organisms.
A
wide
variety
prokaryotic
eukaryotic
microorganisms,
known
microalgae,
have
the
potential
be
economical
ecologically
sustainable
manufacture
biofuels
bio-hydrogen,
biodiesel,
bio-oils,
bio-syngas.
By
using
contemporary
bioengineering
techniques,
innate
algae
produce
biomass
superior
quality
may
enhanced.
In
algal
biotechnology,
directed
genome
modification
via
RNA-guided
endonucleases
is
a
new
approach.
CRISPR/Cas
systems
recently
been
frequently
used
modify
genetic
makeup
several
aquatic
freshwater
microalgae.
The
majority
research
Cas9-driven
Type
II
system,
one
two
classes
six
unique
kinds
CRISPR
systems,
specifically
target
desired
genes
algae,
knock
them
out
down,
or
both.
Using
technology
makeup,
microalgae
produced
more
increased
lipid
content.
review
highlights
attempts
made
so
far
modification,
discusses
prospects
for
developing
platform
large-scale
identifies
opportunities
challenges
development
distribution
CRISPR/Cas9
components.
Язык: Английский
Harnessing NMR technology for enhancing field crop improvement: applications, challenges, and future perspectives
Metabolomics,
Год журнала:
2025,
Номер
21(2)
Опубликована: Фев. 20, 2025
Язык: Английский
Target prediction of potential candidate miRNAs from Oryza sativa to silence the Pyricularia oryzae genome in rice blast
Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Сен. 18, 2024
Rice
(Oryza
sativa)
is
a
staple
food
for
billions
of
people
across
the
globe,
that
feeds
nearly
three-quarters
human
population
on
Earth,
particularly
in
Asian
countries.
yield
has
been
drastically
reduced
and
severely
affected
by
various
biotic
abiotic
stresses,
especially
pathogens.
Controlling
attack
such
pathogens
matter
immediate
concern
as
losses
rice
crops
could
deprive
millions
lives
nourishment
worldwide.
Pyricularia
oryzae
one
pathogen
considered
major
disease
because
its
worldwide
geographic
distribution.
P.
belongs
to
kingdom
fungi,
causes
blast
ultimately
adversely
affecting
crop.
Keeping
view
this
alarming
scenario,
present
study
was
designed
so
identifications
genome-encoded
miRNAs
Oryza
sativa
were
employed
target
silence
genome
oryzae.
This
accomplished
computational
analysis
algorithms
related
miRNA
prediction.
Four
prediction
i.e.,
psRNATarget,
RNA22,
miRanda,
RNAhybrid
utilized
investigation.
The
consensus
among
created
discover
six
from
O.
with
conservation
site
fully
evaluated
discovery
these
novel
paved
strong
way
toward
control
rice.
It
will
open
doors
further
research
field
gene
silencing
These
can
be
future
experimentation
create
constructs
regarding
crops.
In
future,
would
surely
helpful
development
resistant
varieties.
Язык: Английский
INFLUENCE OF VARIOUS WEED MANAGEMENT PRACTICES ON WEEDS AND YIELD COMPONENTS OF PEA (PISUM SATIVUM L.)
Biological and Clinical Sciences Research Journal,
Год журнала:
2024,
Номер
2024(1), С. 1357 - 1357
Опубликована: Дек. 8, 2024
A
field
experiment
was
conducted
throughout
the
winter
cropping
season
of
year
2018,
at
New
Developmental
Research
Farm
(NDRF),
University
Agriculture-Peshawar,
to
assess
efficiency
techniques
for
weed
control
on
suppression,
and
yield
components
edible
pea.
The
research
designed,
using
a
Randomized
Complete
Block
Design,
with
13
treatments,
which
were
repeated
3
times.
applied
treatments
comprised;
Mulches
(Eucalyptus
leaves,
biomass,
poplar
leaves),
herbicides
(S-metolachlor,
Pendimethalin,
Haloxyfop-p-methyl,
Quizalofop-p-ethyl),
hand-weeded
plots
(hand
weeding
20
DAS,
hand
40
60
two
times
30
DAS),
weed-free,
weedy
check(untreated).
Parameters
documented
included;
density
(m-²),
fresh
biomass
(kg
ha-¹),
seeds
pod-1,
hundred
seed
weight
(g),
CBR.
Based
our
outcomes,
check,
had
highest
(40.1
m-²),
(441.67
kg
whereas
weed-free
treatment
lowest
values
both
parameters.
Likewise,
in
terms
agronomic
parameters,
produced
(8.10
pod-1),
(53.33
g),
(4352
ha-¹).
While,
check
treatment,
exhibited
these
use
Pendimethalin
cost-benefit
ratio
(CBR),
while
mulching
These
findings
suggest
that
various
methods,
twice
weeding,
application
S-metolachlor
are
effective
controlling
weeds,
enhancing
yield-related
traits
Язык: Английский