Antioxidant production promotes defense mechanism and different gene expression level in Zea mays under abiotic stress
Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Март 26, 2024
Abstract
The
growth
and
productivity
of
maize
are
severely
affected
by
soil
salinity.
crucial
determinants
for
the
future
performance
plants
productive
seed
germination
seedling
establishment;
however,
both
stages
liable
to
For
grain,
is
an
economically
significant
crop
sensitive
abiotic
stresses.
However,
little
known
about
defense
responses
salinity-induced
antioxidant
oxidative
stress
in
maize.
In
our
work,
commercially
available
variety
Raka-Poshi
was
grown
pots
30
days
under
greenhouse
conditions.
To
evaluate
salt-induced
oxidative/antioxidant
salt
0,
25,
50,
75,
100
150
mM
concentrations,
treatments
were
provided
using
sodium
chloride
(NaCl).
All
biochemical
indices
calculated
all
NaCl
while
drought
induced
up
50%
irrigation
water.
After
germination,
leaves
collected
measurement
lipid
peroxidase
or
malondialdehyde
(MDA),
glutathione
reductase
(GR),
guaiacol
(GPOD),
hydrogen
peroxide
(H
2
O
),
superoxide
dismutase
(SOD),
lipoxygenase
(LOX),
catalase
(CAT),
ascorbate
(APOD)
glutathione-
S
-transferase
(GST).
results
revealed
a
47%
reduction
have
shown
that
successive
increase
concentrations
caused
production.
With
concentration
stress,
lower
levels
H
,
SOD,
MDA
detected
leaves.
regarding
morphology
seedlings
indicated
root
length
shoot
applications
root-to-shoot
weights
found
be
increased
decreased
conditions
During
gene
expression
analysis
collectively
indicate
that,
conditions,
nine
mentioned
enzyme-related
genes
consistently
downregulated.
Язык: Английский
DNA damage in inhabitants exposed to heavy metals near Hudiara drain, Lahore, Pakistan
Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Апрель 10, 2024
The
current
study
was
conducted
on
the
inhabitants
living
in
area
adjacent
to
Hudiara
drain
using
bore
water
and
vegetables
drain.
Toxic
heavy
metals
badly
affect
human
health
because
of
industrial
environmental
contamination.
Particularly
hundreds
millions
individuals
globally
have
faced
consequences
consuming
food
tainted
with
pollutants.
Concentrations
blood
were
elevated
drainings
Lahore
city,
Pakistan,
due
highly
polluted
effluents.
determined
effects
high
levels
(Cd,
Cu,
Zn,
Fe,
Pb,
Ni,
Hg,
Cr)
residents
draining
area,
including
serum
MDA,
8-Isoprostane,
8-hydroxyguanosine,
creatinine
levels.
An
absorption
spectrophotometer
used
determine
wate
water,
drinking
soil,
plants
beings
sampleas
ELISA
kits
assess
level
8-Isoprostane
plasma
level.
Waste
samples,
irrigation
Soil
Plants
samples
specimens
adult
different
weights
ages
collected
from
(Laloo
Mohanwal),
control
obtained
unpolluted
site
Sheiikhpura,
60
km
away
site.
damage
cell
membrane
DNA
structures,
increasing
creatinine,
8-Isoprostane.
contaminated
vegetables,
resulting
increased
Isoprostane,
8-hydroxy-2-guanosine
compared
group.
In
addition,.
This
also
investigated
metal
concentrations
meat
milk
buffaloes,
cows,
goats.
meat,
cow
showed
highest
Cd,
Fe
Mn
concentrations.
also,
cows
exhibited
Cu
results
highlight
species-specific
variations
accumulation,
emphasizing
need
for
targeted
monitoring
address
potential
risks.
significant
difference
between
two
groups
i.e.,
group
affected
group,
all
traits
respondents
(weight,
age,
values
8-hydroxyguaniosine,
level).
Pearson's
correlation
coefficient
calculated.
has
shown
that
or
not
significantly
correlated
so
it
is
independent
age.
proved
selected
villages
Laloo
Mohanwal,
excess
body
damages
increases
8-hydroxyguaniosine.
As
a
result,
National
international
cooperation
must
take
major
steps
exposure
metals.
Язык: Английский
Genome wide identification of phenylalanine ammonia-lyase (PAL) gene family in Cucumis sativus (cucumber) against abiotic stress
BMC Genomic Data,
Год журнала:
2024,
Номер
25(1)
Опубликована: Авг. 26, 2024
Phenylalanine
ammonia
lyase
(PAL)
is
a
widely
studied
enzyme
in
plant
biology
due
to
its
role
connecting
primary
metabolism
secondary
phenylpropanoid
metabolism,
significantly
influencing
growth,
development,
and
stress
response.
Although
PAL
genes
have
been
extensively
various
species
but
their
exploration
cucumber
has
limited.
This
study
successfully
identified
11
CsPAL
Cucumis
sativus
(cucumber).
These
were
categorized
based
on
conserved
sequences
revealing
patterns
through
MEME
analysis
multiple
sequence
alignment.
Interestingly,
cis-elements
related
found
the
promoter
regions
of
genes,
indicating
involvement
responding
abiotic
stress.
Furthermore,
these
gene's
promoters
contained
components
associated
with
light,
development
hormone
responsiveness.
suggests
that
they
may
roles
developmental
processes.
MicroRNAs
as
key
regulators
for
playing
crucial
modulating
expression.
discovery
underscores
complex
regulatory
network
involved
plant's
response
conditions.
The
influence
microRNAs
further
highlights
complicated
mechanisms
plants
use
manage
Gene
expression
analyzed
using
RNA-seq
data.
significant
upregulation
CsPAL9
during
HT3h
(heat
3
h)
heightened
both
CsPAL7
under
HT6h
6
transcriptome
suggest
potential
cucumber's
tolerance
heat
comprehensive
investigation
aims
enhance
our
understanding
gene
family's
versatility,
offering
valuable
insights
advancements
genetics.
Язык: Английский
Evaluation of the Molecular and Phenotypic Performance of Transgenic Rice Genotypes by Using RAPD Technology
D. M. Majeed,
Mohammed Hussein Noor Al-jana,
Eman Noaman Ismail
и другие.
IOP Conference Series Earth and Environmental Science,
Год журнала:
2025,
Номер
1449(1), С. 012071 - 012071
Опубликована: Фев. 1, 2025
Abstract
Knowledge
of
genetic
basis
stress
tolerance
could
be
helpful
for
a
successful
breeding
program.
So,
the
study
was
conducted
to
estimate
molecular
and
phenotypic
performance
several
genotype
transgenic
rice.
The
seeds
ten
genotypes
two
local
varieties
as
control
were
cultivated
selection
best
with
high
economic
yields
under
saline
conditions.
A
Split-Plot
Design
three
replications
used
conduct
experiment
number
three.
Yield
yield
related
traits
well
parameters
recorded.
G21
significant
in
terms
fertile
panicles
(11.48
panicle.
plant
−1
),
seed
weight
(20.85
g),
harvest
index
(45.21%)
G12
grain
per
(21.44
biological
(51.46
g).
Regarding
salinity
levels,
6
dS
level
excels
all
characteristics.
As
interaction
between
both
factors
(genotypes
×
levels),
excelled
levels
(3,
9
dS/m)
accounting
25.38,
21.14,
17.81
g
,
respectively.
results
also
revealed
existence
affinity
studied
conditions,
where
highest
value
reached
0.9048
G18
lowest
Jasmine
varieties.
Overall,
showed
field
salt
conditions
characters
future
selection.
Язык: Английский
Assessment of bioremediation potential of Calotropis procera and Nerium oleander for sustainable management of vehicular released metals in roadside soils
Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Апрель 18, 2024
Land
transportation
is
a
major
source
of
heavy
metal
contamination
along
the
roadside,
posing
significant
risks
to
human
health
through
inhalation,
oral
ingestion,
and
dermal
contact.
Therefore,
this
study
has
been
designed
determine
concentrations
vehicular
released
metals
(Cd,
Pb,
Ni,
Cu)
in
roadside
soil
leaves
two
commonly
growing
native
plant
species
(Calotropis
procera
Nerium
oleander).Two
busy
roads
i.e.,
Lahore-Okara
road
(N-5)
Okara-Faisalabad
(OFR)
Punjab,
Pakistan,
were
selected
for
study.
The
data
collected
from
five
sites
each
during
four
seasons.
Control
samples
~
50
m
away
road.
content
i.e.
lead
(Pb),
cadmium
(Cd)
nickel
(Ni)
copper
(Cu)
determined
by
using
Atomic
Absorption
Spectrophotometer
(AAS).
Significantly
high
amount
all
studied
observed
both
contrast
control
ones.
mean
concentration
ranged
as
Cd
(2.20-6.83
mg/kg),
Pb
(4.53-15.29
Ni
(29.78-101.26
Cu
(61.68-138.46
mg/kg)
(0.093-0.53
(4.31-16.34
(4.13-16.34
(2.98-32.74
mg/kg).
Among
roads,
higher
was
noted
N-5
Significant
temporal
variations
also
roads.
order
different
seasons
summer
>
autumn
spring
winter.
Furthermore,
accumulation
potential
Calotropis
than
that
oleander.
sustainable
management
contamination,
plantation
recommended
roadsides.
Язык: Английский
Target prediction of potential candidate miRNAs from Oryza sativa to silence the Pyricularia oryzae genome in rice blast
Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Сен. 18, 2024
Rice
(Oryza
sativa)
is
a
staple
food
for
billions
of
people
across
the
globe,
that
feeds
nearly
three-quarters
human
population
on
Earth,
particularly
in
Asian
countries.
yield
has
been
drastically
reduced
and
severely
affected
by
various
biotic
abiotic
stresses,
especially
pathogens.
Controlling
attack
such
pathogens
matter
immediate
concern
as
losses
rice
crops
could
deprive
millions
lives
nourishment
worldwide.
Pyricularia
oryzae
one
pathogen
considered
major
disease
because
its
worldwide
geographic
distribution.
P.
belongs
to
kingdom
fungi,
causes
blast
ultimately
adversely
affecting
crop.
Keeping
view
this
alarming
scenario,
present
study
was
designed
so
identifications
genome-encoded
miRNAs
Oryza
sativa
were
employed
target
silence
genome
oryzae.
This
accomplished
computational
analysis
algorithms
related
miRNA
prediction.
Four
prediction
i.e.,
psRNATarget,
RNA22,
miRanda,
RNAhybrid
utilized
investigation.
The
consensus
among
created
discover
six
from
O.
with
conservation
site
fully
evaluated
discovery
these
novel
paved
strong
way
toward
control
rice.
It
will
open
doors
further
research
field
gene
silencing
These
can
be
future
experimentation
create
constructs
regarding
crops.
In
future,
would
surely
helpful
development
resistant
varieties.
Язык: Английский