International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
26(1), С. 270 - 270
Опубликована: Дек. 31, 2024
Hymenoptera
venom
allergy
(HVA)
is
a
potentially
life-threatening
condition,
making
accurate
diagnosis
crucial
for
identifying
significant
IgE
sensitizations
and
enabling
effective
immunotherapy.
In
this
review,
we
provide
detailed
overview
of
biomarkers
the
molecular
IgE-mediated
hypersensitivity
to
insect
venoms
in
clinical
practice,
present,
structured
manner,
their
importance
differentiating
genuine
versus
cross-sensitizations
using
different
diagnostic
procedures.
Updated
algorithms
are
provided,
along
with
advantages
limitations
approaches.
Geographical
variations
rare
species
may
pose
further
challenges
diagnosing
treating
HVA,
adding
complexity
HVA
management.
This
review
informs
readers
about
performing
tailored
diagnostics
based
on
allergen
subsequent
treatment
strategies.
Chemical Society Reviews,
Год журнала:
2020,
Номер
49(24), С. 9121 - 9153
Опубликована: Янв. 1, 2020
The
present
review
focuses
on
the
use
of
Metal–Organic
Frameworks,
(MOFs)
highlighting
most
recent
developments
in
biological
field
and
as
bio-sensors.
Current Issues in Molecular Biology,
Год журнала:
2023,
Номер
45(7), С. 5481 - 5493
Опубликована: Июнь 29, 2023
Diagnosis
of
allergic
diseases
is
a
complex,
multi-stage
process.
It
often
requires
the
use
various
diagnostic
tools.
The
in
vitro
diagnostics
(IVD),
which
includes
laboratory
tests,
one
stages
this
Standard
tests
include
measurement
serum
concentration
specific
immunoglobulin
E
(sIgE)
for
selected
allergens,
full
allergen
extracts
and/or
single
components
(molecules).
IgE
sIgE
to
called
molecular
allergy
diagnosis.
During
standard
process,
models
immunochemical
are
used,
enable
allergens
(one-parameter
singleplex)
or
many
different
(multi-parameter
multiplex)
test.
Currently,
there
test
kits
available,
validated
IVD,
differ
method
type
and
profile.
aim
manuscript
present
technical
aspects
related
modern
diagnostics,
especially
area
diagnostics.
Russian Journal of Biotherapy,
Год журнала:
2025,
Номер
24(1), С. 10 - 26
Опубликована: Апрель 15, 2025
Background
.
The
allergic
diseases
are
growing
everywhere.
exact
diagnosis
of
allergens
becomes
more
and
critical
because
it
significantly
affects
the
further
treatment
patient,
including
question
about
using
allergen-specific
immunotherapy.
There
several
laboratory
instrumental
tests
among
which
skin
most
available.
Despite
its
low
cost,
this
method
is
characterized
by
a
large
percentage
false-positive
and,
importantly,
false-negative
results.
In
addition,
that
can
be
detected
limited.
Aim.
To
conduct
comparative
analysis
effectiveness
diagnostic
accuracy
allergen
chips
from
different
manufacturers
to
evaluate
their
applicability
in
molecular
diseases.
Results
ImmunoCAP
ISAC
Allergy
Explorer
(ALEX)
gradually
occupying
niche
Both
methods
have
benefits
disadvantages.
high
cost
difficulties
supplying
foreign
materials
sharply
limit
widespread
use
allergy
profiles
Russia.
On
other
hand,
personalized
medicine
requires
doctors
patient-centered
care,
completed
existing
tools.
panels
for
various
regions
Russia
solve
problem.
Identifying
common
clinically
significant
molecules
each
region
will
enable
accurate
allergies
patient
treatment.
This
improve
medical
care
quality
increase
therapy
effectiveness.
Conclusion.
Developing
implementing
new
creating
optimal
important
steps
towards
improving
These
measures
help
clinicians
accurately
identify
relevant
develop
individual
plans
ultimately
leading
better
public
health
lower
economic
costs
treating
allergies.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(15), С. 8210 - 8210
Опубликована: Июль 27, 2024
IgE-mediated
wheat
allergy
can
take
on
various
forms,
including
childhood
food
to
wheat,
wheat-dependent
exercise-induced
anaphylaxis
in
young
adults,
baker’s
respiratory
allergy/asthma
workers
exposed
flour
inhalation,
and
contact
urticaria
that
is
caused
by
hydrolyzed
proteins
some
cosmetics,
sometimes
associated
with
a
allergy.
Singleplex
multiplex
immunoassays
detect
specific
IgE
antibodies
allergenic
molecular
biomarkers
such
as
omega-5
gliadin
Tri
19,
lipid
transfer
protein
14,
alpha-amylase
inhibitors.
The
fluorescence
enzyme
immunoassay
capsulated
cellulose
polymer
solid-phase
coupled
allergens
commonly
used
singleplex
assay.
Multiplex
methods
include
the
ELISA-based
macroarray
using
nano-bead
technology
microarray
polymer-coated
slides.
Another
promising
diagnostic
tool
basophil
activation
test
performed
other
types.
Detailed
comprehension
of
structural
immunological
features
numerous
significant
clinical
settings
imperative
for
advancing
allergies.
Expert Review of Clinical Immunology,
Год журнала:
2019,
Номер
15(8), С. 849 - 856
Опубликована: Июль 15, 2019
Introduction:
Food-dependent,
exercise-induced
anaphylaxis
(FDEIA)
is
a
rare
type
of
with
growing
incidence.
Although
the
precise
mechanism
by
which
patient
reacts
only
in
combination
culprit
food
and
cofactors
are
not
currently
understood,
many
advances
diagnosis
management
have
been
made
since
their
first
description.
Areas
covered:
A
literature
search
PubMed
was
performed
to
review
FDEIA.
Clinicians
should
high
level
suspicion
for
identification
foods
involved.
Component-resolved
more
accurate
provocation
tests
revolutionized
accuracy.
Management
easy
involves
educating
evict
exposure
that
elicit
anaphylaxis,
how
act
treat
if
reaction
occurs.
Expert
opinion:
FDEIA
misdiagnosed
authors
believe
there
patients
labelled
as
idiopathic
unnecessary
evictions
poor
quality
life
because
fear
an
imminent
reaction.
Due
recent
diagnostic
tools
use
monoclonal
antibodies
prophylaxis
persistent
cases,
can
better
prognosis
improving
families.
Clinical & Experimental Allergy,
Год журнала:
2020,
Номер
51(4), С. 604 - 613
Опубликована: Ноя. 11, 2020
The
measurement
of
specific
IgE
to
allergenic
extracts
and
molecules
in
patients
with
allergic
rhinitis
(AR)
is
crucial
for
a
precise
diagnosis
further
immunotherapy.
Companies
providing
vitro
diagnostic
methods
allergology
continuously
strive
the
optimization
modernization
such
methods.
A
new
generation
automated
allergy
tests
based
on
chemiluminescence
detection
paramagnetic
microparticles
now
available,
possible
advantages
sample
volume,
cost-effectiveness
avoidance
sample-related
interference.To
test
whether
sIgE
antibody
levels
obtained
singleplex
chemiluminescent
method
have
good
agreement
corresponding
results
"gold
standard"
test.We
tested
sera
from
368
AR
patients.
Specific
(kU/L)
comprehensive
panel
15
allergen
6
were
ImmunoCAP®
(Thermo
Fisher
Scientific
Inc,
Phadia
AB,
Uppsala,
Sweden)
NOVEOS™
(HYCOR®
Biomedical,
Garden
Grove,
CA,
USA).
We
evaluated
qualitative
quantitative
performance
NOVEOS
system
matching
outcome
ImmunoCAP
each
examined
allergens.In
relation
ImmunoCAP,
overall
sensitivity
specificity
90.8%
(95%
CI
=
88.6-92.7)
96.2%
93.9-97.8),
respectively.
These
values
higher
when
only
considered
(sensitivity
98.7%
[95%
96.4%-99.7%];
94.2%
88.4%-97.6%])
lower
87.6%
84.7%-90.2%];
97%
94.4%-98.6%]).
Spearman's
correlation
between
data
set
both
≥
0.1
kU/L
cut-off
was
0.84
(p
<
.001).The
presented
comparisons
testing
serum
antibodies
against
range
21
allergens.
This
novel
immunoassay
using
4
µl
per
appears
be
robust
reliable
can,
therefore,
used
as
an
aid
diagnosis.
