EAACIMolecular Allergology User's Guide 2.0

Pediatric Allergy and Immunology, Год журнала: 2023, Номер 34(S28)

Опубликована: Март 1, 2023

Abstract Since the discovery of immunoglobulin E (IgE) as a mediator allergic diseases in 1967, our knowledge about immunological mechanisms IgE‐mediated allergies has remarkably increased. In addition to understanding immune response and clinical symptoms, allergy diagnosis management depend strongly on precise identification elicitors reaction. past four decades, innovations bioscience technology have facilitated production well‐defined, highly pure molecules for component‐resolved (CRD), allowing personalized disease individual patients. The first edition “EAACI Molecular Allergology User's Guide” (MAUG) 2016 rapidly became key reference clinicians, scientists, interested readers with background allergology, immunology, biology, medicine. Nevertheless, field molecular allergology is moving fast, after 6 years, new EAACI Taskforce was established provide an updated document. Guide 2.0 summarizes state‐of‐the‐art information allergen molecules, their relevance, application diagnostic algorithms practice. It designed both, clinicians guiding health care professionals through overwhelming list different available testing. Further, it provides relevance allergenic gives overview basic test formats, tests measure exposure.

Язык: Английский

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Going over Fungal Allergy: Alternaria alternata and Its Allergens

Journal of Fungi, Год журнала: 2023, Номер 9(5), С. 582 - 582

Опубликована: Май 18, 2023

Fungal allergy is the third most frequent cause of respiratory pathologies and related to a poor prognosis asthma. The genera Alternaria Cladosporium are frequently associated with allergic diseases, being one highest prevalence sensitization. alternata an outdoor fungus whose spores disseminate in warm dry air, reaching peak levels temperate summers. can also be found damp insufficiently ventilated houses, causing what known as sick building syndrome. Thus, exposure fungal allergens occur outdoors indoors. However, not only but fragments contain detectable amounts may function aeroallergenic sources. Allergenic extracts hyphae still use for diagnosis treatment diseases variable standardised, they often random mixture allergenic ingredients casual impurities. has been difficult, knowledge about new stuck. number described Fungi remains almost constant while Plantae Animalia kingdoms. Given Alt 1 unique allergen eliciting symptoms, component-resolved strategies should applied diagnose allergy. To date, twelve A. accepted WHO/IUIS Allergen Nomenclature Subcommittee, many them enzymes: 4 (disulfide isomerase), 6 (enolase), 8 (mannitol de-hydrogenase), 10 (aldehyde dehydrogenase), 13 (glutathione-S-transferase) MnSOD (Mn superoxide dismutase), others have structural regulatory functions such 5 12, 3, 7. 9 unknown. Other four included other medical databases (e.g., Allergome): NTF2, TCTP, 70 kDa. Despite major allergen, allergens, enolase, or MnSOD, 14 suggested panel

Язык: Английский

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Understanding of lipid transfer protein sensitization patterns and its clinical significance in children

Allergy and Asthma Proceedings, Год журнала: 2024, Номер 45(2), С. 120 - 127

Опубликована: Март 1, 2024

Background: Lipid transfer proteins (LTP) are the most common food allergens in Mediterranean region. Objective: The study aimed to investigate co-sensitization patterns and cluster relationships between LTP allergen molecules across a broad range of allergen-specific sensitization patterns, clinical outcomes eastern children. Methods: Among 496 children evaluated for multiple sensitizations with multiplex testing, 105 (21%) 16 different were analyzed. Clinical reactivity was examined based on clear-cut history immunoglobulin E mediated symptoms (oral allergy syndrome [OAS], systemic reactions, anaphylaxis). Results: All included sensitive LTPs, but 56% pollen LTPs. number OAS 12 59, respectively, no cofactors reported. Pru p 3 (74%) Cor 8 (66%). Significant correlations observed heatmap other than Par j 2 Tri 14. Overall, associated increased age molecule positivity. Conclusion: In region, 21% and/or found have sensitization; however, almost half reported reactivity. hierarchical pathway highlights that distinct can act as primary sensitizers. is linked increasing numbers positivity age.

Язык: Английский

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Multiplex Assays in Allergy Diagnosis: Allergy Explorer 2 versus ImmunoCAP ISAC E112i

Diagnostics, Год журнала: 2024, Номер 14(10), С. 976 - 976

Опубликована: Май 8, 2024

ImmunoCAP ISAC E112i (ISAC) and Allergy Explorer 2 (ALEX2) detect specific immunoglobulin E (IgE) reactivity. Both multiplex assays contain molecular allergens ALEX2 additionally includes allergen extracts inhibitors that block the binding of IgE to cross-reacting carbohydrate determinants (CCDs). This study aimed compare performance by determining reactivity against and/or components using qualitative, semiquantitative, quantitative analyses all comparable in sera from 216 participants recruited South Tyrol/Italy. For extract sensitization ALEX2, analysis revealed negative corresponding 18.4% at least one positive component 81.6% cases. ISAC, results were 23.5% 76.5% cases, respectively. The CCD inhibitor eliminated CCD-positive signals detected 88.5% Based on values 0.3–14.9 ISU or kUA/L, there was good agreement between although showed higher than ISAC. addition allergen-extract tests resulted detection more sensitizations with alone. In range <15 may be effective detecting sensitizations.

Язык: Английский

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Natural history of sesame allergy in pediatric patients: Insight from a retrospective analysis

Pediatric Allergy and Immunology, Год журнала: 2025, Номер 36(1)

Опубликована: Янв. 1, 2025

Sesame allergy (SA) is a growing concern because of its association with severe reactions and the limited knowledge long-term outcomes.

Язык: Английский

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Fundamentals of laboratory diagnostics in allergology

Allergo Journal International, Год журнала: 2025, Номер unknown

Опубликована: Фев. 26, 2025

Язык: Английский

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Grundlagen der Labordiagnostik in der Allergologie

Allergo Journal, Год журнала: 2025, Номер 34(2), С. 16 - 29

Опубликована: Март 1, 2025

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ALEX versus ISAC multiplex array in analyzing food allergy in atopic children

Clinical and Molecular Allergy, Год журнала: 2022, Номер 20(1)

Опубликована: Авг. 27, 2022

ALEX multiplex array is a relatively new allergy test which analyses more than 120 allergen extracts and 170 molecular components. ISAC the most used studied to date, offering 112 In ten atopic children with multiple food allergies good agreement was observed between sIgE results for nearly all shared Presence of larger number allergens in could help clinicians improve personalized dietary advice. However positive sensitizations unknown clinical relevance were found by ALEX, potentially increasing complexity. Pediatric allergists should be aware this, especially young (severe) eczema who have not introduced sorts yet.

Язык: Английский

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Evolution Toward Chip-Based Arrays in the Laboratory Diagnosis of Human Allergic Disease

The Journal of Allergy and Clinical Immunology In Practice, Год журнала: 2023, Номер 11(10), С. 2991 - 2999

Опубликована: Авг. 18, 2023

Язык: Английский

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Prevalence of nut allergen sensitization among children in central Poland

Advances in Dermatology and Allergology, Год журнала: 2023, Номер 40(1), С. 40 - 46

Опубликована: Янв. 1, 2023

The diagnostics of plant-derived food allergy may be challenging. However, the recognition sensitization patterns in defined populations, especially children, is clinically relevant as it enables use secondary prophylaxis to prevent life-threatening complications.To investigate rates and nut allergens children from central Poland.The retrospective assessment concerned data 598 diagnosed a single centre due suspected allergy. analysis included results component-based multiparametric assay Allergy Explorer2 (ALEX2).The particular varied among patients, depending on their age type. any was found 67% whereas hazelnut peanut most common (56% 55% all respectively). Hazelnut predominant every age, its prevalence increased with while detected more than half individuals groups, except for teenagers (44% cases). Among molecules Cor 1.04 prevalent (74% sensitized children), - Ara h 1 (65% patients). simultaneous hazelnut, walnut (two or them) almost entire group.Component-based differentiation between primary cross-reactive detects co-sensitization. clinical relevance latter observation remarkable co-sensitization increases risk reactions even trace contamination.

Язык: Английский

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