Biomarkers for the Molecular Diagnosis of IgE-Mediated Hymenoptera Venom Allergy in Clinical Practice DOI Open Access
Florin‐Dan Popescu, Mariana Vieru, Darío Antolín‐Amérigo

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 26(1), P. 270 - 270

Published: Dec. 31, 2024

Hymenoptera venom allergy (HVA) is a potentially life-threatening condition, making accurate diagnosis crucial for identifying significant IgE sensitizations and enabling effective immunotherapy. In this review, we provide detailed overview of biomarkers the molecular IgE-mediated hypersensitivity to insect venoms in clinical practice, present, structured manner, their importance differentiating genuine versus cross-sensitizations using different diagnostic procedures. Updated algorithms are provided, along with advantages limitations approaches. Geographical variations rare species may pose further challenges diagnosing treating HVA, adding complexity HVA management. This review informs readers about performing tailored diagnostics based on allergen subsequent treatment strategies.

Language: Английский

Metal–organic frameworks: a future toolbox for biomedicine? DOI
Ricardo F. Mendes, Flávio Figueira, José P. Leite

et al.

Chemical Society Reviews, Journal Year: 2020, Volume and Issue: 49(24), P. 9121 - 9153

Published: Jan. 1, 2020

The present review focuses on the use of Metal–Organic Frameworks, (MOFs) highlighting most recent developments in biological field and as bio-sensors.

Language: Английский

Citations

185
Selected Technical Aspects of Molecular Allergy Diagnostics DOI Creative Commons
Kinga Lis, Zbigniew Bartuzi

Current Issues in Molecular Biology, Journal Year: 2023, Volume and Issue: 45(7), P. 5481 - 5493

Published: June 29, 2023

Diagnosis of allergic diseases is a complex, multi-stage process. It often requires the use various diagnostic tools. The in vitro diagnostics (IVD), which includes laboratory tests, one stages this Standard tests include measurement serum concentration specific immunoglobulin E (sIgE) for selected allergens, full allergen extracts and/or single components (molecules). IgE sIgE to called molecular allergy diagnosis. During standard process, models immunochemical are used, enable allergens (one-parameter singleplex) or many different (multi-parameter multiplex) test. Currently, there test kits available, validated IVD, differ method type and profile. aim manuscript present technical aspects related modern diagnostics, especially area diagnostics.

Language: Английский

Citations

14
Allergic bronchopulmonary aspergillosis: A multidisciplinary review DOI Creative Commons

Amytis Roboubi,

Camille Audousset, Émilie Fréalle

et al.

Journal de Mycologie Médicale, Journal Year: 2023, Volume and Issue: 33(3), P. 101392 - 101392

Published: May 4, 2023

Language: Английский

Citations

13
Allergen chip in molecular diagnostic: from cohort analysis to personalized patient care DOI
Ksenja Riabova, Evgenii Kozlov, Daria Trifonova

et al.

Russian Journal of Biotherapy, Journal Year: 2025, Volume and Issue: 24(1), P. 10 - 26

Published: April 15, 2025

Background . The allergic diseases are growing everywhere. exact diagnosis of allergens becomes more and critical because it significantly affects the further treatment patient, including question about using allergen-specific immunotherapy. There several laboratory instrumental tests among which skin most available. Despite its low cost, this method is characterized by a large percentage false-positive and, importantly, false-negative results. In addition, that can be detected limited. Aim. To conduct comparative analysis effectiveness diagnostic accuracy allergen chips from different manufacturers to evaluate their applicability in molecular diseases. Results ImmunoCAP ISAC Allergy Explorer (ALEX) gradually occupying niche Both methods have benefits disadvantages. high cost difficulties supplying foreign materials sharply limit widespread use allergy profiles Russia. On other hand, personalized medicine requires doctors patient-centered care, completed existing tools. panels for various regions Russia solve problem. Identifying common clinically significant molecules each region will enable accurate allergies patient treatment. This improve medical care quality increase therapy effectiveness. Conclusion. Developing implementing new creating optimal important steps towards improving These measures help clinicians accurately identify relevant develop individual plans ultimately leading better public health lower economic costs treating allergies.

Language: Английский

Citations

0
Validation of a commercial allergen microarray platform for specific immunoglobulin E detection of respiratory and plant food allergens DOI
Paola Leonor Quan, Marina Sabaté Brescó,

Carmen D’Amelio

et al.

Annals of Allergy Asthma & Immunology, Journal Year: 2021, Volume and Issue: 128(3), P. 283 - 290.e4

Published: Dec. 1, 2021

Language: Английский

Citations

22
The use of microarray and other multiplex technologies in the diagnosis of allergy DOI

Behnam Keshavarz,

Thomas A.E. Platts‐Mills, Jeffrey M. Wilson

et al.

Annals of Allergy Asthma & Immunology, Journal Year: 2021, Volume and Issue: 127(1), P. 10 - 18

Published: Jan. 14, 2021

Language: Английский

Citations

19
Allergenic Biomarkers in the Molecular Diagnosis of IgE-Mediated Wheat Allergy DOI Open Access

Mariana Preda,

Florin‐Dan Popescu, Εmilia Vassilopoulou

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(15), P. 8210 - 8210

Published: July 27, 2024

IgE-mediated wheat allergy can take on various forms, including childhood food to wheat, wheat-dependent exercise-induced anaphylaxis in young adults, baker’s respiratory allergy/asthma workers exposed flour inhalation, and contact urticaria that is caused by hydrolyzed proteins some cosmetics, sometimes associated with a allergy. Singleplex multiplex immunoassays detect specific IgE antibodies allergenic molecular biomarkers such as omega-5 gliadin Tri 19, lipid transfer protein 14, alpha-amylase inhibitors. The fluorescence enzyme immunoassay capsulated cellulose polymer solid-phase coupled allergens commonly used singleplex assay. Multiplex methods include the ELISA-based macroarray using nano-bead technology microarray polymer-coated slides. Another promising diagnostic tool basophil activation test performed other types. Detailed comprehension of structural immunological features numerous significant clinical settings imperative for advancing allergies.

Language: Английский

Citations

2
Diagnosis and prevention of food-dependent exercise-induced anaphylaxis DOI

Filipe Benito-Garcia,

Ignacio J. Ansotegui, Mário Morais‐Almeida

et al.

Expert Review of Clinical Immunology, Journal Year: 2019, Volume and Issue: 15(8), P. 849 - 856

Published: July 15, 2019

Introduction: Food-dependent, exercise-induced anaphylaxis (FDEIA) is a rare type of with growing incidence. Although the precise mechanism by which patient reacts only in combination culprit food and cofactors are not currently understood, many advances diagnosis management have been made since their first description. Areas covered: A literature search PubMed was performed to review FDEIA. Clinicians should high level suspicion for identification foods involved. Component-resolved more accurate provocation tests revolutionized accuracy. Management easy involves educating evict exposure that elicit anaphylaxis, how act treat if reaction occurs. Expert opinion: FDEIA misdiagnosed authors believe there patients labelled as idiopathic unnecessary evictions poor quality life because fear an imminent reaction. Due recent diagnostic tools use monoclonal antibodies prophylaxis persistent cases, can better prognosis improving families.

Language: Английский

Citations

22
Validation study of a new chemiluminescent singleplex IgE assay in a set of Italian allergic rhinitis patients DOI Creative Commons
Ekaterina Potapova,

Daniel Bauersachs,

Valeria Villella

et al.

Clinical & Experimental Allergy, Journal Year: 2020, Volume and Issue: 51(4), P. 604 - 613

Published: Nov. 11, 2020

The measurement of specific IgE to allergenic extracts and molecules in patients with allergic rhinitis (AR) is crucial for a precise diagnosis further immunotherapy. Companies providing vitro diagnostic methods allergology continuously strive the optimization modernization such methods. A new generation automated allergy tests based on chemiluminescence detection paramagnetic microparticles now available, possible advantages sample volume, cost-effectiveness avoidance sample-related interference.To test whether sIgE antibody levels obtained singleplex chemiluminescent method have good agreement corresponding results "gold standard" test.We tested sera from 368 AR patients. Specific (kU/L) comprehensive panel 15 allergen 6 were ImmunoCAP® (Thermo Fisher Scientific Inc, Phadia AB, Uppsala, Sweden) NOVEOS™ (HYCOR® Biomedical, Garden Grove, CA, USA). We evaluated qualitative quantitative performance NOVEOS system matching outcome ImmunoCAP each examined allergens.In relation ImmunoCAP, overall sensitivity specificity 90.8% (95% CI = 88.6-92.7) 96.2% 93.9-97.8), respectively. These values higher when only considered (sensitivity 98.7% [95% 96.4%-99.7%]; 94.2% 88.4%-97.6%]) lower 87.6% 84.7%-90.2%]; 97% 94.4%-98.6%]). Spearman's correlation between data set both ≥ 0.1 kU/L cut-off was 0.84 (p < .001).The presented comparisons testing serum antibodies against range 21 allergens. This novel immunoassay using 4 µl per appears be robust reliable can, therefore, used as an aid diagnosis.

Language: Английский

Citations

17
Highly sensitive optoelectrical biosensor for multiplex allergy diagnosis DOI

Salvador Mas,

Ahmed Badran,

M.J. Juárez

et al.

Biosensors and Bioelectronics, Journal Year: 2020, Volume and Issue: 166, P. 112438 - 112438

Published: July 15, 2020

Language: Английский

Citations

16