The Agro-industrial Byproduct Wheat Bran as an Inducer for Alkaline Protease (ALK-PR23) Production by Pschyrotolerant Lysinibacillus sphaericus Strain AA6 EMCCN3080
Waste and Biomass Valorization,
Год журнала:
2023,
Номер
15(4), С. 1943 - 1958
Опубликована: Окт. 11, 2023
Abstract
The
current
study
aims
to
exploit
the
zero-cost
inducer
wheat
bran
(WB),
an
agro-industrial
byproduct,
for
production
of
alkaline
protease
(ALK-PR23)
by
hyper
producer
psychrotolerant
Lysinibacillus
sphaericus
Strain
AA6
EMCCN3080
first
time
ever.
Incubation
temperature
(25
°C),
yeast
extract
concentration,
agitation
speed
(150
rpm),
and
aeration
ratio
[1
volume
(liquid):5
(Erlenmeyer
flask)]
provoked
ALK-PR23
production;
OVAT
inferences.
pH,
extract,
(NH
4
)
2
SO
levels
substantively
triggered
as
deduced
from
Plackett–Burman
design.
(3
days)
WB
[2%
(w/v)]
were
optimal
values
inducing
positive
significant
influence
on
conferred
steepest
ascent
experiments.
Yeast
(0.446%
w/v),
(0.339%
pH
(6.872)
prompted
(592.5
U/mL)
with
impressive
98-fold
enhancement;
Box-Behnken
design
ridge
path
implications.
laboratory
validation
model
achieved
100%
predicted
value.
Laboratory
data
would
present
eco-friendly,
cheap,
efficient
approach
towards
concurrent
recycling
massive
L.
.
Present
greatly
encourage
unveiling
biochemical
characteristics
in
prospective
studies.
Graphical
Язык: Английский
The fermentation optimization for alkaline protease production by Bacillus subtilis BS-QR-052
Biao Sun,
Kai Zou,
Yingqing Zhao
и другие.
Frontiers in Microbiology,
Год журнала:
2023,
Номер
14
Опубликована: Дек. 19, 2023
Introduction
Proteases
exhibit
a
wide
range
of
applications,
and
among
them,
alkaline
proteases
have
become
prominent
area
research
due
to
their
stability
in
highly
environments.
To
optimize
the
production
yield
activity
proteases,
researchers
are
continuously
exploring
different
fermentation
conditions
culture
medium
components.
Methods
In
this
paper,
protease
(EC
3.4.21.14)
by
Bacillus
subtilis
BS-QR-052
were
optimized,
effect
nutrition
was
investigated.
Based
on
single-variable
experiments,
Plackett–Burman
design
used
explore
significant
factors,
then
optimized
conditions,
as
well
interaction
between
these
evaluated
response
surface
methodology
through
Box–Behnken
design.
Results
discussion
The
results
showed
that
1.03%
corn
syrup
powder,
0.05%
MgSO
4
,
8.02%
inoculation
volume,
1:1.22
vvm
airflow
rate,
0.5%
starch,
MnSO
180
rpm
agitation
speed,
36°C
temperature,
8.0
initial
pH
96
h
incubation
time
predicted
be
optimal
conditions.
enzyme
estimated
approximately
1787.91
U/mL,
whereas
subsequent
experimental
validation
confirmed
it
reached
1780.03
while
500
L
scale-up
1798.33
U/mL.
This
study
for
B.
systematic
data
analysis,
increased
300.72%
its
original
level.
established
model
predicting
validated,
achieving
significantly
higher
levels
enzymatic
activity.
findings
provide
valuable
references
further
enhancing
protease,
thereby
holding
substantial
practical
significance
economic
benefits
industrial
applications.
Язык: Английский
Optimization of protease production using Bacillus velezensis through response surface methodology and investigating its applications as stain remover
Systems Microbiology and Biomanufacturing,
Год журнала:
2024,
Номер
4(4), С. 1313 - 1322
Опубликована: Июль 2, 2024
Язык: Английский
Molecular Characterization and Optimization of Alkaline Protease Production by Bacillus cereus LS23B
Asian Journal of Biotechnology and Bioresource Technology,
Год журнала:
2023,
Номер
9(4), С. 1 - 13
Опубликована: Ноя. 10, 2023
The
study
aimed
to
determine
the
conditions
leading
maximum
protease
production
using
submerged
fermentation
and
detecting
presence
of
genes
in
bacteria.
This
is
necessary
meet
increasing
demand
for
enzymes
industrial
market.
ability
bacterial
isolate
produce
was
evaluated
through
primary
screening.
After
that,
morphological
characterization,
biochemical
tests,
16S
rRNA
analysis
were
done
identify
strain.
To
confirm
gene-encoding
enzyme,
(npr)
gene
primer
amplified
a
polymerase
chain
reaction.
pH,
incubation
duration,
temperature,
carbon,
nitrogen
sources
studied.
Others
include
metal
ions,
substrate
concentration,
agitation
speed.
strain
has
100%
similarity
Bacillus
cereus
A9,
while
encoding
confirmed
with
positive
bands
951bp.
enzyme
optimally
produced
at
40
oC
pH
9
after
72
h
incubation.
Starch,
gelatin,
1
%
1mM
K+,
speed
160
rpm
fully
supported
production.
npr
confirmed.
Also,
optimization
reveals
that
LS23B
can
be
used
large-scale
production,
which
may
different
biotechnological
applications.
Язык: Английский
Production of Alkaline Serine Protease from Bacillus subtilis MTCC 8601
Research Journal of Pharmacy and Technology,
Год журнала:
2024,
Номер
unknown, С. 4161 - 4168
Опубликована: Сен. 24, 2024
Alkaline
serine
protease
is
a
proteolytic
enzyme
having
wide
array
of
industrial
applications.
These
proteases
have
high
enzymatic
activity
at
pH
ranging
between
8-12
and
temperature
38℃,
hence
finding
its
importance
in
industries
such
as
detergent
where
pHs
reach
10.7
due
to
the
presence
caustic
soda
other
alkaline
materials.
Production
from
Bacillus
subtilis
MTCC
8601
on
establishment
shake
flask
fermentation
protocol
has
been
carried
out
this
study.
Qualitative
analysis
using
Biuret
test
conducted,
quantification
crude
extract
was
Lowry’s
method
quantitative
which
found
be
0.847
mg/ml.
Further,
determined
sensitivity
Ninhydrin
amino
acid
detection
for
results
were
3.51
mg/ml
when
Bovine
serum
albumin
used
substrate
1.419
Gelatin
substrate,
unit
0.445
μg/
mol
min
0.180
each
respectively.
Язык: Английский