Regulation of metabolism, stress response, and sod1 activity by cytosolic thioredoxins in yeast depends on growth phase DOI Creative Commons
Cecilia Picazo, C. Alicia Padilla, Brian McDonagh

и другие.

Advances in Redox Research, Год журнала: 2023, Номер 9, С. 100081 - 100081

Опубликована: Сен. 20, 2023

Reactive Oxygen Species (ROS) can be harmful compounds that cause damage to macromolecules like lipids, proteins, and DNA when their levels exceed cellular defense mechanisms. Cells have protection ROS detoxification systems, including thioredoxin glutaredoxin counteract oxidative stress. The role of cytosolic system (cTRX) was investigated in different growth phases using a mutant strain lacking both TRX1 TRX2. showed defect survival during the non-dividing state or stationary phase. glutathione, an antioxidant, mutants were higher total reduced indicating increase response. also protein-bound suggesting compensatory mechanism counter balance Proteomic analysis revealed changes expression various proteins absence thioredoxins. Upregulated exponential mainly related stress response metabolism. Downregulated associated with glycerol metabolism, glycolysis, ATP synthesis. These indicated redox imbalance caused by Further focused on reversible oxidation cysteine residues proteins. Several identified cysteines susceptible oxidation, status affected Notably, 146 Superoxide Dismutase 1 (Sod1) more oxidized phase, while ribosomal seen only Overall, this study provides insights into growth, aging, maintaining balance, protecting against as well its impact SOD1 activity glutathionylation.

Язык: Английский

Beneficial and Detrimental Effects of Reactive Oxygen Species on Lifespan: A Comprehensive Review of Comparative and Experimental Studies DOI Creative Commons
Hazel J. Shields, Annika Traa, Jeremy M. Van Raamsdonk

и другие.

Frontiers in Cell and Developmental Biology, Год журнала: 2021, Номер 9

Опубликована: Фев. 11, 2021

Aging is the greatest risk factor for a multitude of diseases including cardiovascular disease, neurodegeneration and cancer. Despite decades research dedicated to understanding aging, mechanisms underlying aging process remain incompletely understood. The widely-accepted free radical theory (FRTA) proposes that accumulation oxidative damage caused by reactive oxygen species (ROS) one primary causes aging. To define relationship between ROS there have been two main approaches: comparative studies measure outcomes related across with different lifespans, experimental modulate levels within single using either genetic or pharmacologic approach. Comparative shown are inversely correlated lifespan. While these in general support FRTA, this type experiment can only demonstrate correlation, not causation. Experimental involving manipulation model organisms generally interventions increase tend decrease lifespan, while However, also multiple examples which opposite observed: increasing results extended longevity, decreasing shortened contradict predictions experiments performed very limited number species, all relatively short Overall, data suggest lifespan complex, both beneficial detrimental effects on longevity depending conditions. Accordingly, difficult generalize tree life.

Язык: Английский

Процитировано

369
Glutathione and glutathione-dependent enzymes: From biochemistry to gerontology and successful aging DOI
Domenico Lapenna

Ageing Research Reviews, Год журнала: 2023, Номер 92, С. 102066 - 102066

Опубликована: Сен. 7, 2023

Язык: Английский

Процитировано

78
Redox regulation in lifespan determination DOI Creative Commons
Christina Karagianni, Daphne Bazopoulou

Journal of Biological Chemistry, Год журнала: 2024, Номер 300(3), С. 105761 - 105761

Опубликована: Фев. 15, 2024

One of the major challenges that remain in fields aging and lifespan determination concerns precise roles reactive oxygen species (ROS) play these processes. ROS, including superoxide hydrogen peroxide, are constantly generated as byproducts aerobic metabolism, well response to endogenous exogenous cues. While ROS accumulation oxidative damage were long considered constitute some main causes age-associated decline, more recent studies reveal a signaling role process. In fact, spatiotemporal manner, can trigger beneficial cellular responses promote longevity healthy aging. this review, we discuss importance timing compartmentalization external internal perturbations organismal redox regulated pathways.

Язык: Английский

Процитировано

12
Redox proteomics reveal a role for peroxiredoxinylation in stress protection DOI

Gerhard Seisenbacher,

Zrinka Raguz Nakic, Eva Borràs

и другие.

Cell Reports, Год журнала: 2025, Номер 44(2), С. 115224 - 115224

Опубликована: Янв. 22, 2025

Язык: Английский

Процитировано

1
Peroxiredoxins couple metabolism and cell division in an ultradian cycle DOI
Prince Saforo Amponsah, Galal Yahya, Jannik Zimmermann

и другие.

Nature Chemical Biology, Год журнала: 2021, Номер 17(4), С. 477 - 484

Опубликована: Фев. 11, 2021

Язык: Английский

Процитировано

54
Fmp40 ampylase regulates cell survival upon oxidative stress by controlling Prx1 and Trx3 oxidation DOI Creative Commons
Suchismita Masanta, Aneta Więsyk, Chiranjit Panja

и другие.

Redox Biology, Год журнала: 2024, Номер 73, С. 103201 - 103201

Опубликована: Май 21, 2024

Reactive oxygen species (ROS), play important roles in cellular signaling, nonetheless are toxic at higher concentrations. Cells have many interconnected, overlapped or backup systems to neutralize ROS, but their regulatory mechanisms remain poorly understood. Here, we reveal an essential role for mitochondrial AMPylase Fmp40 from budding yeast regulating the redox states of 1-Cys peroxiredoxin Prx1, which is only protein shown H

Язык: Английский

Процитировано

7
Peroxiredoxin promotes longevity and H2O2-resistance in yeast through redox-modulation of protein kinase A DOI Creative Commons
Friederike Roger, Cecilia Picazo, Wolfgang Reiter

и другие.

eLife, Год журнала: 2020, Номер 9

Опубликована: Июль 14, 2020

Peroxiredoxins are H2O2 scavenging enzymes that also carry out signaling and chaperone functions. In yeast, the major cytosolic peroxiredoxin, Tsa1 is required for both promoting resistance to extending lifespan upon caloric restriction. We show here effects these functions not by H2O2, but repressing nutrient Ras-cAMP-PKA pathway at level of protein kinase A (PKA) enzyme. stimulates sulfenylation cysteines in PKA catalytic subunit a significant proportion subunits glutathionylated on two cysteine residues. Redox modification conserved Cys243 inhibits phosphorylation Thr241 activation loop enzyme activity, preventing can overcome sensitivity Tsa1-deficient cells. Results support model aging where pathways constitute hubs integrating information from multiple aging-related conduits, including peroxiredoxin-dependent response H2O2.

Язык: Английский

Процитировано

33
Overview of yeast environmental stress response pathways and the development of tolerant yeasts DOI Open Access

Nai-Xin Lin,

Yan Xu, Xiao‐Wei Yu

и другие.

Systems Microbiology and Biomanufacturing, Год журнала: 2021, Номер 2(2), С. 232 - 245

Опубликована: Сен. 15, 2021

Язык: Английский

Процитировано

33
Insights on the role of l-lactate as a signaling molecule in skin aging DOI Creative Commons
Salvatore Chirumbolo, Dario Bertossi, Pierre J. Magistretti

и другие.

Biogerontology, Год журнала: 2023, Номер 24(5), С. 709 - 726

Опубликована: Янв. 28, 2023

L-lactate is a catabolite from the anaerobic metabolism of glucose, which plays paramount role as signaling molecule in various steps cell survival. Its activity, master tuner many mechanisms underlying aging process, for example skin, still presumptive, however its crucial position complex cross-talk between mitochondria and process survival, should suggest that may be not simple waste product but fine regulator aging/survival machinery, probably via mito-hormesis. Actually, emerging evidence highlighting ROS are skin health, including wound repair, renewal aging. The ROS, superoxide anion, hydrogen peroxide, nitric oxide, play both beneficial detrimental roles depending upon their levels cellular microenvironment. Physiological essential cutaneous health repair process. Aberrant redox activity drives chronic disease elderly. On contrary, impaired modulation, due to enhanced generation and/or reduced antioxidant defense, suppresses healing promoting lymphatic/vascular endothelial apoptosis death. This review tries elucidate this issue.

Язык: Английский

Процитировано

12
Impact of Hydrogen Peroxide on Protein Synthesis in Yeast DOI Creative Commons
Cecilia Picazo, Mikael Molin

Antioxidants, Год журнала: 2021, Номер 10(6), С. 952 - 952

Опубликована: Июнь 12, 2021

Cells must be able to respond and adapt different stress conditions maintain normal function. A common response is the global inhibition of protein synthesis. Protein synthesis an expensive process consuming much cell's energy. Consequently, it tightly regulated conserve resources. One these oxidative stress, resulting from accumulation reactive oxygen species (ROS) mainly produced by mitochondria but also other intracellular sources. utilize a variety antioxidant systems protect against ROS, directing signaling adaptation responses at lower levels and/or detoxification as increase preclude damage. In this review, we focus on role hydrogen peroxide, H2O2, molecule regulating levels, including transcription various parts translation process, e.g., initiation, elongation, termination ribosome recycling.

Язык: Английский

Процитировано

26