AoPrdx2 Regulates Oxidative Stress, Reactive Oxygen Species, Trap Formation, and Secondary Metabolism in Arthrobotrys oligospora DOI Creative Commons
Na Zhao, Meichen Zhu, Qianqian Liu

et al.

Journal of Fungi, Journal Year: 2024, Volume and Issue: 10(2), P. 110 - 110

Published: Jan. 28, 2024

Prdx2 is a peroxiredoxin (Prx) family protein that protects cells from attack via reactive oxygen species (ROS), and it has an important role in improving the resistance scavenging capacity of ROS fungi. Arthrobotrys oligospora widespread nematode-trapping fungus can produce three-dimensional nets to capture kill nematodes. In this study, AoPrdx2, homologous Prx5, was investigated A. gene disruption, phenotypic analysis, metabolomics. The deletion Aoprdx2 resulted increase number mycelial septa reduction nuclei spore yield. Meanwhile, absence increased sensitivity oxidative stresses, whereas ∆Aoprdx2 mutant strain higher levels than wild-type (WT) strain. particular, inactivation severely influenced trap formation pathogenicity; traps produced by remarkably reduced rings less WT addition, abundance metabolites significantly downregulated compared with These results indicate AoPrdx2 plays indispensable ROS, morphogenesis, secondary metabolism.

Language: Английский

Beneficial and Detrimental Effects of Reactive Oxygen Species on Lifespan: A Comprehensive Review of Comparative and Experimental Studies DOI Creative Commons
Hazel J. Shields, Annika Traa, Jeremy M. Van Raamsdonk

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2021, Volume and Issue: 9

Published: Feb. 11, 2021

Aging is the greatest risk factor for a multitude of diseases including cardiovascular disease, neurodegeneration and cancer. Despite decades research dedicated to understanding aging, mechanisms underlying aging process remain incompletely understood. The widely-accepted free radical theory (FRTA) proposes that accumulation oxidative damage caused by reactive oxygen species (ROS) one primary causes aging. To define relationship between ROS there have been two main approaches: comparative studies measure outcomes related across with different lifespans, experimental modulate levels within single using either genetic or pharmacologic approach. Comparative shown are inversely correlated lifespan. While these in general support FRTA, this type experiment can only demonstrate correlation, not causation. Experimental involving manipulation model organisms generally interventions increase tend decrease lifespan, while However, also multiple examples which opposite observed: increasing results extended longevity, decreasing shortened contradict predictions experiments performed very limited number species, all relatively short Overall, data suggest lifespan complex, both beneficial detrimental effects on longevity depending conditions. Accordingly, difficult generalize tree life.

Language: Английский

Citations

369
Glutathione and glutathione-dependent enzymes: From biochemistry to gerontology and successful aging DOI
Domenico Lapenna

Ageing Research Reviews, Journal Year: 2023, Volume and Issue: 92, P. 102066 - 102066

Published: Sept. 7, 2023

Language: Английский

Citations

78
Redox regulation in lifespan determination DOI Creative Commons
Christina Karagianni, Daphne Bazopoulou

Journal of Biological Chemistry, Journal Year: 2024, Volume and Issue: 300(3), P. 105761 - 105761

Published: Feb. 15, 2024

One of the major challenges that remain in fields aging and lifespan determination concerns precise roles reactive oxygen species (ROS) play these processes. ROS, including superoxide hydrogen peroxide, are constantly generated as byproducts aerobic metabolism, well response to endogenous exogenous cues. While ROS accumulation oxidative damage were long considered constitute some main causes age-associated decline, more recent studies reveal a signaling role process. In fact, spatiotemporal manner, can trigger beneficial cellular responses promote longevity healthy aging. this review, we discuss importance timing compartmentalization external internal perturbations organismal redox regulated pathways.

Language: Английский

Citations

12
Redox proteomics reveal a role for peroxiredoxinylation in stress protection DOI

Gerhard Seisenbacher,

Zrinka Raguz Nakic, Eva Borràs

et al.

Cell Reports, Journal Year: 2025, Volume and Issue: 44(2), P. 115224 - 115224

Published: Jan. 22, 2025

Language: Английский

Citations

1
Peroxiredoxins couple metabolism and cell division in an ultradian cycle DOI
Prince Saforo Amponsah, Galal Yahya, Jannik Zimmermann

et al.

Nature Chemical Biology, Journal Year: 2021, Volume and Issue: 17(4), P. 477 - 484

Published: Feb. 11, 2021

Language: Английский

Citations

54
Fmp40 ampylase regulates cell survival upon oxidative stress by controlling Prx1 and Trx3 oxidation DOI Creative Commons
Suchismita Masanta, Aneta Więsyk, Chiranjit Panja

et al.

Redox Biology, Journal Year: 2024, Volume and Issue: 73, P. 103201 - 103201

Published: May 21, 2024

Reactive oxygen species (ROS), play important roles in cellular signaling, nonetheless are toxic at higher concentrations. Cells have many interconnected, overlapped or backup systems to neutralize ROS, but their regulatory mechanisms remain poorly understood. Here, we reveal an essential role for mitochondrial AMPylase Fmp40 from budding yeast regulating the redox states of 1-Cys peroxiredoxin Prx1, which is only protein shown H

Language: Английский

Citations

7
Peroxiredoxin promotes longevity and H2O2-resistance in yeast through redox-modulation of protein kinase A DOI Creative Commons
Friederike Roger, Cecilia Picazo, Wolfgang Reiter

et al.

eLife, Journal Year: 2020, Volume and Issue: 9

Published: July 14, 2020

Peroxiredoxins are H2O2 scavenging enzymes that also carry out signaling and chaperone functions. In yeast, the major cytosolic peroxiredoxin, Tsa1 is required for both promoting resistance to extending lifespan upon caloric restriction. We show here effects these functions not by H2O2, but repressing nutrient Ras-cAMP-PKA pathway at level of protein kinase A (PKA) enzyme. stimulates sulfenylation cysteines in PKA catalytic subunit a significant proportion subunits glutathionylated on two cysteine residues. Redox modification conserved Cys243 inhibits phosphorylation Thr241 activation loop enzyme activity, preventing can overcome sensitivity Tsa1-deficient cells. Results support model aging where pathways constitute hubs integrating information from multiple aging-related conduits, including peroxiredoxin-dependent response H2O2.

Language: Английский

Citations

33
Overview of yeast environmental stress response pathways and the development of tolerant yeasts DOI Open Access

Nai-Xin Lin,

Yan Xu, Xiao‐Wei Yu

et al.

Systems Microbiology and Biomanufacturing, Journal Year: 2021, Volume and Issue: 2(2), P. 232 - 245

Published: Sept. 15, 2021

Language: Английский

Citations

32
Insights on the role of l-lactate as a signaling molecule in skin aging DOI Creative Commons
Salvatore Chirumbolo, Dario Bertossi, Pierre J. Magistretti

et al.

Biogerontology, Journal Year: 2023, Volume and Issue: 24(5), P. 709 - 726

Published: Jan. 28, 2023

L-lactate is a catabolite from the anaerobic metabolism of glucose, which plays paramount role as signaling molecule in various steps cell survival. Its activity, master tuner many mechanisms underlying aging process, for example skin, still presumptive, however its crucial position complex cross-talk between mitochondria and process survival, should suggest that may be not simple waste product but fine regulator aging/survival machinery, probably via mito-hormesis. Actually, emerging evidence highlighting ROS are skin health, including wound repair, renewal aging. The ROS, superoxide anion, hydrogen peroxide, nitric oxide, play both beneficial detrimental roles depending upon their levels cellular microenvironment. Physiological essential cutaneous health repair process. Aberrant redox activity drives chronic disease elderly. On contrary, impaired modulation, due to enhanced generation and/or reduced antioxidant defense, suppresses healing promoting lymphatic/vascular endothelial apoptosis death. This review tries elucidate this issue.

Language: Английский

Citations

12
Impact of Hydrogen Peroxide on Protein Synthesis in Yeast DOI Creative Commons
Cecilia Picazo, Mikael Molin

Antioxidants, Journal Year: 2021, Volume and Issue: 10(6), P. 952 - 952

Published: June 12, 2021

Cells must be able to respond and adapt different stress conditions maintain normal function. A common response is the global inhibition of protein synthesis. Protein synthesis an expensive process consuming much cell's energy. Consequently, it tightly regulated conserve resources. One these oxidative stress, resulting from accumulation reactive oxygen species (ROS) mainly produced by mitochondria but also other intracellular sources. utilize a variety antioxidant systems protect against ROS, directing signaling adaptation responses at lower levels and/or detoxification as increase preclude damage. In this review, we focus on role hydrogen peroxide, H2O2, molecule regulating levels, including transcription various parts translation process, e.g., initiation, elongation, termination ribosome recycling.

Language: Английский

Citations

26