Conversion of (poly)phenolic compounds in food fermentations by lactic acid bacteria: Novel insights into metabolic pathways and functional metabolites

Current Research in Food Science, Год журнала: 2023, Номер 6, С. 100448 - 100448

Опубликована: Янв. 1, 2023

Lactobacillaceae are among the major fermentation organisms in most food fermentations but metabolic pathways for conversion of (poly)phenolic compounds by lactobacilli have been elucidated only past two decades. Hydroxycinnamic and hydroxybenzoic acids metabolized separate enzymes which include multiple esterases, decarboxylases hydroxycinnamic acid reductases. Glycosides phenolic including flavonoids glycosidases, some dedicated to glycosides plant phytochemicals rather than oligosaccharides. Metabolism often differs from metabolism vitro, likely reflecting diversity unknown stimuli that induce expression genes. Current knowledge will facilitate strategies achieve improved quality targeted compounds.

Язык: Английский

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The honeybee microbiota and its impact on health and disease

Nature Reviews Microbiology, Год журнала: 2023, Номер 22(3), С. 122 - 137

Опубликована: Дек. 4, 2023

Язык: Английский

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Host-derived organic acids enable gut colonization of the honey bee symbiont Snodgrassella alvi

Nature Microbiology, Год журнала: 2024, Номер 9(2), С. 477 - 489

Опубликована: Янв. 15, 2024

Diverse bacteria can colonize the animal gut using dietary nutrients or by engaging in microbial crossfeeding interactions. Less is known about role of host-derived enabling bacterial colonization. Here we examined metabolic interactions within evolutionary ancient symbiosis between honey bee (Apis mellifera) and core microbiota member Snodgrassella alvi. This betaproteobacterium incapable metabolizing saccharides, yet colonizes presence a sugar-only diet. Using comparative metabolomics,

Язык: Английский

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Genomic insights into the phylogeny and biomass-degrading enzymes of rumen ciliates

The ISME Journal, Год журнала: 2022, Номер 16(12), С. 2775 - 2787

Опубликована: Авг. 19, 2022

Язык: Английский

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Distinct Roles of Honeybee Gut Bacteria on Host Metabolism and Neurological Processes

Microbiology Spectrum, Год журнала: 2022, Номер 10(2)

Опубликована: Март 10, 2022

The honeybee possesses a limited number of bacterial phylotypes that play essential roles in host metabolism, hormonal signaling, and feeding behavior. However, the contribution individual gut members shaping brain profiles remains unclear. By generating gnotobiotic bees which were mono-colonized by single bacterium, we revealed different species regulated specific modules metabolites hemolymph. Circulating involved carbohydrate glycerophospholipid metabolism pathways mostly Gilliamella, while Lactobacillus Firm4 Firm5 mainly altered amino acid pathways. We then analyzed transcriptomes with these three bacteria. These showed distinctive gene expression profiles, genes related to olfactory functions labor division upregulated Lactobacillus. Interestingly, differentially spliced brains largely overlapped those unresponsive social stimuli. enriched neural development synaptic transmission. bacteria neurotransmitter levels brain. In particular, dopamine serotonin, show inhibitory effects on sensory sensitivity bees, downregulated bacteria-colonized bees. proboscis extension response normal microbiota is for taste-related behavior honeybees, suggesting potential interactions among host's physiology. Our findings provide fundamental insights into diverse likely contribute neurological processes. IMPORTANCE simple host-restricted community contributes metabolic health its host, symbionts remain elusive. found colonization bee regulates distinct circulating carbohydrate, acid, colonized display transcriptomic crucial behaviors labor. Alternative splicing disordered also mediated. presence promotes sucrose major neurotransmitters being demonstrate how affect behaviors, highlighting gut-brain connections important neurobiological physiological states.

Язык: Английский

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Impact of intraspecific variation in insect microbiomes on host phenotype and evolution

The ISME Journal, Год журнала: 2023, Номер 17(11), С. 1798 - 1807

Опубликована: Сен. 2, 2023

Abstract Microbes can be an important source of phenotypic plasticity in insects. Insect physiology, behaviour, and ecology are influenced by individual variation the microbial communities held within insect gut, reproductive organs, bacteriome, other tissues. It is becoming increasingly clear how microbiome for fitness, expansion into novel ecological niches, environments. These investigations have garnered heightened interest recently, yet a comprehensive understanding intraspecific assembly function these insect-associated shape insects still lacking. Most research focuses on core associated with species ignores variation. We argue that among driver evolution, we provide examples showing such influence fitness health insects, invasions, their persistence new environments, responses to global environmental changes.

Язык: Английский

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Starter Culture Development and Innovation for Novel Fermented Foods

Annual Review of Food Science and Technology, Год журнала: 2023, Номер 15(1), С. 211 - 239

Опубликована: Дек. 6, 2023

Interest in fermented foods is increasing because are promising solutions for more secure food systems with an increased proportion of minimally processed plant and a smaller environmental footprint. These developments also pertain to novel which no traditional template exists, raising the question how develop starter cultures such fermentations. This review establishes framework that integrates scientific knowledge selection suitable cultures. Safety considerations, use organisms fermentations, link phylogeny metabolic properties provide criteria culture selection. Such approaches can select microbial strains have health benefits. A science-based approach development substantially advance their value through systems, products health-promoting microbes, provision improve human health.

Язык: Английский

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A longitudinal field study of commercial honey bees shows that non-native probiotics do not rescue antibiotic treatment, and are generally not beneficial

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Янв. 23, 2024

Abstract Probiotics are widely used in agriculture including commercial beekeeping, but there is little evidence supporting their effectiveness. Antibiotic treatments can greatly distort the gut microbiome, reducing its protective abilities and facilitating growth of antibiotic resistant pathogens. Commercial beekeepers regularly apply antibiotics to combat bacterial infections, often followed by an application non-native probiotics advertised ease impact antibiotic-induced dysbiosis. We tested whether affect microbiome or disease prevalence, rescue negative effects induced found no difference markers probiotic recovery associated with treatment. A colony-level oxytetracycline tylosin produced immediate decrease size, over longer-term, very different persistent dysbiotic on composition membership hindgut microbiome. Our results demonstrate lack effect rescue, detail duration character states resulting from antibiotics, highlight importance for honeybee health.

Язык: Английский

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Species divergence in gut-restricted bacteria of social bees

Proceedings of the National Academy of Sciences, Год журнала: 2022, Номер 119(18)

Опубликована: Апрель 25, 2022

Host-associated microbiomes, particularly gut often harbor related but distinct microbial lineages, how this diversity arises and is maintained not well understood. A prerequisite for lineage diversification reproductive isolation imposed by barriers to gene flow. In host-associated microbes, genetic recombination can be disrupted confinement different hosts, example following host speciation, or niche partitioning within the same host. Taking advantage of simple microbiome social bees, we explore two groups gut-associated bacteria, Gilliamella Snodgrassella, which have evolved 80 million y with honey bees bumble bees. Our analyses sequenced genomes show that these lineages diversified into discrete populations limited Divergence has occurred between symbionts species and, in some cases, symbiont a single individual. Populations acquired genes adapt specific hosts ecological niches; example, differ markedly abilities degrade dietary polysaccharides use resulting sugar components. Using engineered fluorescent bacteria vivo, localize hindgut regions, corresponding differences their spatially concentrated nitrogenous wastes hosts. findings bee diversify due also spatial individual leading

Язык: Английский

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Social Interaction is Unnecessary for Hindgut Microbiome Transmission in Honey Bees: The Effect of Diet and Social Exposure on Tissue-Specific Microbiome Assembly

Microbial Ecology, Год журнала: 2022, Номер 85(4), С. 1498 - 1513

Опубликована: Май 2, 2022

Abstract Honey bees are a model for host–microbial interactions with experimental designs evolving towards conventionalized worker bees. Research on gut microbiome transmission and assembly has examined only fraction of factors associated the colony hive environment. Here, we studied effects diet social isolation tissue-specific bacterial fungal colonization midgut two key hindgut regions. We found that both treatment significantly influenced early explaining similar proportions variation. In agreement previous work, interaction older workers was unnecessary core transmission. Exposure to natural eclosion fresh stored pollen resulted in communities were taxonomically structurally equivalent those produced setting. Stressed diets no or autoclaved decreased abundance diversity, atypical structure variation functionally important bacteria. Without exposure active environment, strain diversity keystone ileum species Gilliamella apicola markedly reduced. These changes larger microbiotas suggesting extended environment plays an antibiotic role establishment. conclude is facultative horizontal 5 6 readily acquired from built diet. Our findings contribute novel insights into influencing maintenance honey bee microbiota facilitate future designs.

Язык: Английский

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