bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Янв. 25, 2023
Abstract
Cells
are
exposed
to
a
wide
variety
of
internal
and
external
stresses.
Whereas
many
studies
have
focused
on
cellular
responses
acute
severe
stresses,
little
is
known
about
how
systems
adapt
sublethal
chronic
Using
mammalian
cells
in
culture,
we
discovered
that
they
mild
stresses
up
two
weeks,
notably
proteotoxic
such
as
heat,
by
increasing
their
size
translation,
thereby
scaling
the
amount
total
protein.
These
adaptations
render
them
more
resilient
persistent
subsequent
We
demonstrate
Hsf1,
well
for
its
role
stress
responses,
required
cell
increase,
molecular
chaperone
Hsp90
essential
coupling
increase
augmented
translation.
term
this
translational
reprogramming
“rewiring
response”,
propose
protective
process
adaptation
contributes
get
older,
failure
promotes
aging.
Frontiers in Oncology,
Год журнала:
2023,
Номер
13
Опубликована: Окт. 24, 2023
The
prevalence
of
gastric
cancer
has
markedly
declined,
but
due
to
the
high
mortality
rates
associated
with
cancer,
it
is
still
a
serious
disease.
preferred
classification
according
Lauren
into
either
intestinal
type,
which
glandular
growth
pattern,
or
diffuse
does
not
have
structures.
Both
types
been
classified
as
adenocarcinomas,
latter
type
based
on
periodic
acid–Schiff
(PAS)
positivity
presumed
reflect
mucin.
However,
presence
mucin
in
contrast
neuroendocrine/enterochromaffin-like
(ECL)
cell
markers,
confirmed
by
immunohistochemistry
and
situ
hybridization.
ECL
cells
are
probably
prone
becoming
cancerous
because
they
do
express
E-cadherin.
Gastric
unique
that
bacterium,
Helicobacter
pylori
,
thought
be
its
main
cause.
H.
predisposes
infected
individuals
only
after
having
caused
oxyntic
atrophy
leading
hypoacidity
hypergastrinemia.
No
single
factor
convincingly
proved
carcinogenic.
It
probable
gastrin
pathogenetic
for
autoimmune
gastritis,
long-term
prolonged
inhibition
acid
secretion.
Hypergastrinemia
induces
hyperplasia,
develops
neuroendocrine
tumors
(NETs)
then
carcinomas
rodents,
sequence
also
described
humans.
During
carcinogenesis,
tumor
lose
specific
traits,
requiring
sensitive
methods
used
recognize
their
origin.
occurrence
may
hopefully
prevented
eradication
at
young
age,
reduced
use
inhibitors
secretion
antagonist
those
previous
infection
gastritis.
Eukaryotic
phytoplankton,
also
known
as
algae,
form
the
basis
of
marine
food
webs
and
drive
carbon
sequestration.
Algae
must
regulate
their
motility
gravitational
sinking
to
balance
access
light
at
surface
nutrients
in
deeper
layers.
However,
regulation
remains
largely
unknown,
especially
motile
species.
Here,
we
quantify
velocities
according
Stokes’
law
diverse
clades
unicellular
microalgae
reveal
cell
size,
density,
nutrient
dependency
velocities.
We
identify
a
algal
species,
Tetraselmis
sp.,
that
sinks
faster
when
starved
due
photosynthesis-driven
accumulation
carbohydrates
loss
intracellular
water,
both
which
increase
density.
Moreover,
is
connected
proliferation
can
respond
multiple
nutrients.
Overall,
our
work
elucidates
how
size
density
environmental
conditions
vertical
migration
algae.
Journal of Biophotonics,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 20, 2025
ABSTRACT
The
lack
of
high‐throughput,
label‐free,
and
intelligent
recognition
models
for
assessing
cell
death
hinders
the
broad
application
analysis
in
chemotherapy
lung
cancer.
We
propose
an
quantitative
detection
technique
deaths.
Using
high‐throughput
phase
imaging
flow
cytometry
to
capture
numerous
label‐free
images
employing
convolutional
neural
networks
(CNN)
characterize
heterogeneity
death.
revealed
through
morphology
features
achieved
interpretability
CNN
using
clustering.
Finally,
classification
reliability
was
validated
by
extracting
from
classified
cells.
This
method,
compared
with
biochemical
methods,
showed
a
correlation
0.92
0.91
autophagy
(Pearson
Cosine
Similarity),
average
error
12.52%
apoptosis
detection.
Our
approach
has
potential
become
valuable
tool
studying
mechanisms
offers
new
perspective
cancer
treatment.
Free-Living
Amoebae
(FLA)
are
widely
distributed
protozoa
in
both
nature
and
artificial
environments
such
as
drinking
water.
Besides
the
ability
of
all
FLA
to
transport
various
pathogenic
microorganisms,
certain
species,
Acanthamoeba
spp.
or
Balamuthia
mandrillaris,
have
intrinsic
abilities
cause
severe
cerebral
infections.
Previous
work
has
shown
an
enrichment
cysts
biofilm
developed
upper
levels
Drinking
Water
Storage
Towers
(DWST),
suggesting
that
differences
densities
may
play
a
role
their
unequal
distribution
water
column.
To
evaluate
this
hypothesis,
model
column
was
created
for
study
used
analyze
vertical
castellanii,
Vermamoeba
vermiformis
mandrillaris
from
0
23
weeks.
Interestingly,
our
data
showed
A.
castellanii
V.
were
enriched
during
aging.
However,
B.
equally
entire
study.
These
results
that,
besides
level
variation
DWST,
some
can
become
less
dense
aging,
which
contributes
water,
thus
levels.
Cells
are
exposed
to
a
wide
variety
of
internal
and
external
stresses.
Although
many
studies
have
focused
on
cellular
responses
acute
severe
stresses,
little
is
known
about
how
systems
adapt
sublethal
chronic
Using
mammalian
cells
in
culture,
we
discovered
that
they
mild
stresses
up
two
weeks,
notably
proteotoxic
such
as
heat,
by
increasing
their
size
translation,
thereby
scaling
the
amount
total
protein.
These
adaptations
render
them
more
resilient
persistent
subsequent
We
demonstrate
Hsf1,
well
for
its
role
stress
responses,
required
cell
increase,
molecular
chaperone
Hsp90
essential
coupling
increase
augmented
translation.
term
this
translational
reprogramming
'rewiring
response',
propose
protective
process
adaptation
contributes
get
older,
failure
promotes
aging.
Advanced NanoBiomed Research,
Год журнала:
2023,
Номер
3(7)
Опубликована: Апрель 15, 2023
Polyethylene
glycol
(PEG)
is
used
in
many
applications,
and
the
clinical
field,
it
one
of
main
components
latest
Covid
vaccines.
Its
wide
use
justified
by
a
relatively
safe
profile
few
known
side
effects.
However,
little
about
biophysical
effects
PEG
on
cells
such
as,
cell
stiffness,
dry
mass,
total
mass.
Herein,
exploiting
digital
holographic
microscope,
an
inertial
picobalance,
nanoindenter,
these
properties
are
characterized
rat
embryonic
fibroblast
exposed
to
different
molecular
weights
PEG.
Immediately
after
first
minutes
exposure
cells,
reduction
mass
can
be
observed
as
well
rapid
fluctuation
weight.
Cell
stiffness
decreases
significantly
48
h,
while
no
important
morphological
changes
observed.
Here,
revealed
how
rapidly
finely
regulated,
highlighting
maintenance
density
primary
importance
cellular
activities.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Окт. 30, 2023
Abstract
Cyanobacteria
are
photosynthetic
organisms
that
play
important
roles
in
carbon
cycling
as
well
promising
bioproduction
chassis.
Here,
we
isolate
two
novel
cyanobacteria,
UTEX
3221
and
3222,
from
a
unique
marine
environment
with
naturally
elevated
CO₂.
We
describe
complete
genome
sequences
for
both
isolates
and,
focusing
on
3222
due
to
its
planktonic
growth
liquid,
characterize
biotechnologically-relevant
biomass
characteristics.
outpaces
other
fast-growing
model
strains
solid
medium.
It
can
double
every
2.35
hours
liquid
medium
grows
high
density
(>31g/L
dry
weight)
batch
culture,
nearly
of
Synechococcus
sp.
PCC
11901,
whose
high-density
was
recently
reported.
In
addition,
sinks
readily,
settling
more
quickly
than
strains,
suggesting
improved
de-watering
biomass.
This
behavior
be
explained
part
by
larger
cell
volume.
These
traits
may
make
compelling
choice
Overall,
find
bio-prospecting
environments
CO₂
uncover
CO₂-metabolizing
Cells
are
exposed
to
a
wide
variety
of
internal
and
external
stresses.
Although
many
studies
have
focused
on
cellular
responses
acute
severe
stresses,
little
is
known
about
how
systems
adapt
sublethal
chronic
Using
mammalian
cells
in
culture,
we
discovered
that
they
mild
stresses
up
two
weeks,
notably
proteotoxic
such
as
heat,
by
increasing
their
size
translation,
thereby
scaling
the
amount
total
protein.
These
adaptations
render
them
more
resilient
persistent
subsequent
We
demonstrate
Hsf1,
well
for
its
role
stress
responses,
required
cell
increase,
molecular
chaperone
Hsp90
essential
coupling
increase
augmented
translation.
term
this
translational
reprogramming
‘rewiring
response’,
propose
protective
process
adaptation
contributes
get
older,
failure
promotes
aging.