The central role of gastrin in gastric cancer

Frontiers in Oncology, Год журнала: 2023, Номер 13

Опубликована: Окт. 24, 2023

The prevalence of gastric cancer has markedly declined, but due to the high mortality rates associated with cancer, it is still a serious disease. preferred classification according Lauren into either intestinal type, which glandular growth pattern, or diffuse does not have structures. Both types been classified as adenocarcinomas, latter type based on periodic acid–Schiff (PAS) positivity presumed reflect mucin. However, presence mucin in contrast neuroendocrine/enterochromaffin-like (ECL) cell markers, confirmed by immunohistochemistry and situ hybridization. ECL cells are probably prone becoming cancerous because they do express E-cadherin. Gastric unique that bacterium, Helicobacter pylori , thought be its main cause. H. predisposes infected individuals only after having caused oxyntic atrophy leading hypoacidity hypergastrinemia. No single factor convincingly proved carcinogenic. It probable gastrin pathogenetic for autoimmune gastritis, long-term prolonged inhibition acid secretion. Hypergastrinemia induces hyperplasia, develops neuroendocrine tumors (NETs) then carcinomas rodents, sequence also described humans. During carcinogenesis, tumor lose specific traits, requiring sensitive methods used recognize their origin. occurrence may hopefully prevented eradication at young age, reduced use inhibitors secretion antagonist those previous infection gastritis.

Язык: Английский

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Cell size, density, and nutrient dependency of unicellular algal gravitational sinking velocities

Science Advances, Год журнала: 2024, Номер 10(27)

Опубликована: Июль 5, 2024

Eukaryotic phytoplankton, also known as algae, form the basis of marine food webs and drive carbon sequestration. Algae must regulate their motility gravitational sinking to balance access light at surface nutrients in deeper layers. However, regulation remains largely unknown, especially motile species. Here, we quantify velocities according Stokes’ law diverse clades unicellular microalgae reveal cell size, density, nutrient dependency velocities. We identify a algal species, Tetraselmis sp., that sinks faster when starved due photosynthesis-driven accumulation carbohydrates loss intracellular water, both which increase density. Moreover, is connected proliferation can respond multiple nutrients. Overall, our work elucidates how size density environmental conditions vertical migration algae.

Язык: Английский

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Plasma membrane folding enables constant surface area-to-volume ratio in growing mammalian cells

Current Biology, Год журнала: 2025, Номер unknown

Опубликована: Март 1, 2025

Язык: Английский

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Label‐Free and Intelligent Cell Death Recognition Toward Lung Cancer Chemotherapy

Journal of Biophotonics, Год журнала: 2025, Номер unknown

Опубликована: Май 20, 2025

ABSTRACT The lack of high‐throughput, label‐free, and intelligent recognition models for assessing cell death hinders the broad application analysis in chemotherapy lung cancer. We propose an quantitative detection technique deaths. Using high‐throughput phase imaging flow cytometry to capture numerous label‐free images employing convolutional neural networks (CNN) characterize heterogeneity death. revealed through morphology features achieved interpretability CNN using clustering. Finally, classification reliability was validated by extracting from classified cells. This method, compared with biochemical methods, showed a correlation 0.92 0.91 autophagy (Pearson Cosine Similarity), average error 12.52% apoptosis detection. Our approach has potential become valuable tool studying mechanisms offers new perspective cancer treatment.

Язык: Английский

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High-throughput single-cell density measurements enable dynamic profiling of immune cell and drug response from patient samples

Nature Biomedical Engineering, Год журнала: 2025, Номер unknown

Опубликована: Май 20, 2025

Язык: Английский

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Influence of the Age of Free-Living Amoeba Cysts on Their Vertical Distribution in a Water Column

Опубликована: Янв. 17, 2024

Free-Living Amoebae (FLA) are widely distributed protozoa in both nature and artificial environments such as drinking water. Besides the ability of all FLA to transport various pathogenic microorganisms, certain species, Acanthamoeba spp. or Balamuthia mandrillaris, have intrinsic abilities cause severe cerebral infections. Previous work has shown an enrichment cysts biofilm developed upper levels Drinking Water Storage Towers (DWST), suggesting that differences densities may play a role their unequal distribution water column. To evaluate this hypothesis, model column was created for study used analyze vertical castellanii, Vermamoeba vermiformis mandrillaris from 0 23 weeks. Interestingly, our data showed A. castellanii V. were enriched during aging. However, B. equally entire study. These results that, besides level variation DWST, some can become less dense aging, which contributes water, thus levels.

Язык: Английский

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Hsf1 and the molecular chaperone Hsp90 support a ‘rewiring stress response’ leading to an adaptive cell size increase in chronic stress

eLife, Год журнала: 2023, Номер 12

Опубликована: Июнь 2, 2023

Cells are exposed to a wide variety of internal and external stresses. Although many studies have focused on cellular responses acute severe stresses, little is known about how systems adapt sublethal chronic Using mammalian cells in culture, we discovered that they mild stresses up two weeks, notably proteotoxic such as heat, by increasing their size translation, thereby scaling the amount total protein. These adaptations render them more resilient persistent subsequent We demonstrate Hsf1, well for its role stress responses, required cell increase, molecular chaperone Hsp90 essential coupling increase augmented translation. term this translational reprogramming 'rewiring response', propose protective process adaptation contributes get older, failure promotes aging.

Язык: Английский

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Fluctuations of Dry and Total Mass of Cells Exposed to Different Molecular Weights of Polyethylene Glycol

Advanced NanoBiomed Research, Год журнала: 2023, Номер 3(7)

Опубликована: Апрель 15, 2023

Polyethylene glycol (PEG) is used in many applications, and the clinical field, it one of main components latest Covid vaccines. Its wide use justified by a relatively safe profile few known side effects. However, little about biophysical effects PEG on cells such as, cell stiffness, dry mass, total mass. Herein, exploiting digital holographic microscope, an inertial picobalance, nanoindenter, these properties are characterized rat embryonic fibroblast exposed to different molecular weights PEG. Immediately after first minutes exposure cells, reduction mass can be observed as well rapid fluctuation weight. Cell stiffness decreases significantly 48 h, while no important morphological changes observed. Here, revealed how rapidly finely regulated, highlighting maintenance density primary importance cellular activities.

Язык: Английский

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Cyanobacteria newly isolated from marine volcanic seeps display rapid sinking and robust, high density growth

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Окт. 30, 2023

Abstract Cyanobacteria are photosynthetic organisms that play important roles in carbon cycling as well promising bioproduction chassis. Here, we isolate two novel cyanobacteria, UTEX 3221 and 3222, from a unique marine environment with naturally elevated CO₂. We describe complete genome sequences for both isolates and, focusing on 3222 due to its planktonic growth liquid, characterize biotechnologically-relevant biomass characteristics. outpaces other fast-growing model strains solid medium. It can double every 2.35 hours liquid medium grows high density (>31g/L dry weight) batch culture, nearly of Synechococcus sp. PCC 11901, whose high-density was recently reported. In addition, sinks readily, settling more quickly than strains, suggesting improved de-watering biomass. This behavior be explained part by larger cell volume. These traits may make compelling choice Overall, find bio-prospecting environments CO₂ uncover CO₂-metabolizing

Язык: Английский

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Hsf1 and the molecular chaperone Hsp90 support a ‘rewiring stress response’ leading to an adaptive cell size increase in chronic stress

eLife, Год журнала: 2023, Номер 12

Опубликована: Дек. 7, 2023

Cells are exposed to a wide variety of internal and external stresses. Although many studies have focused on cellular responses acute severe stresses, little is known about how systems adapt sublethal chronic Using mammalian cells in culture, we discovered that they mild stresses up two weeks, notably proteotoxic such as heat, by increasing their size translation, thereby scaling the amount total protein. These adaptations render them more resilient persistent subsequent We demonstrate Hsf1, well for its role stress responses, required cell increase, molecular chaperone Hsp90 essential coupling increase augmented translation. term this translational reprogramming ‘rewiring response’, propose protective process adaptation contributes get older, failure promotes aging.

Язык: Английский

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